CASP-like protein 1D2 (AtCASPL1D2, At3g06390) is a small tetraspan (four-transmembrane) plasma-membrane protein of the CASP-like (CASPL) subfamily of the MARVEL-related Casparian strip membrane domain protein family (CASPL1 clade). It is one of four CASPLs (with CASPL1B1, CASPL1B2, CASPL1D1) that interact with the aquaporin PIP2;1, is expressed in suberized endodermal cells, and together with CASPL1D1 has a slight, redundant negative role in root endodermal suberization. Its experimentally determined location is the plasma membrane. No specific molecular function has been characterized; it is inferred to act as a membrane scaffold.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005886 plasma membrane | IEA GO_REF:0000120 | ACCEPT | Summary: Correct, well-supported plasma-membrane localization for this multi-pass CASP-like protein. Reason: UniProt annotates a multi-pass cell-membrane protein and the localization is independently confirmed experimentally (IDA, PMID:24920445). Supporting Evidence: file:ARATH/CASPL1D2/CASPL1D2-uniprot.txt SUBCELLULAR LOCATION: Cell membrane |
| GO:0003674 molecular_function | ND GO_REF:0000015 | ACCEPT | Summary: Root-level placeholder reflecting that no specific molecular function has been characterized. Reason: No catalytic or transport activity is known for this CASP-like scaffold protein; the ND root annotation accurately reflects the absence of molecular-function data. Supporting Evidence: file:ARATH/CASPL1D2/CASPL1D2-goa.tsv molecular_function |
| GO:0005634 nucleus | ISM GO_REF:0000122 | REMOVE | Summary: Incorrect nucleus localization for a four-transmembrane plasma-membrane protein; an automated sequence-model artifact. Reason: This CASPL is a multi-pass (four-transmembrane) integral plasma-membrane protein; the nucleus call is an automated sequence-model (ISM) inference that contradicts the UniProt topology and the experimental plasma-membrane localization, and should be removed. Supporting Evidence: file:ARATH/CASPL1D2/CASPL1D2-uniprot.txt SUBCELLULAR LOCATION: Cell membrane file:ARATH/CASPL1D2/CASPL1D2-uniprot.txt Multi-pass membrane |
| GO:0005886 plasma membrane | IDA PMID:24920445 Functional and evolutionary analysis of the CASPARIAN STRIP ... | ACCEPT | Summary: Experimentally determined plasma-membrane localization; the core, well-grounded annotation. Reason: Direct assay in the family-defining study localizes this CASPL to the plasma membrane, consistent with the UniProt multi-pass topology. Supporting Evidence: file:ARATH/CASPL1D2/CASPL1D2-uniprot.txt SUBCELLULAR LOCATION: Cell membrane |
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Download this section (compressed HTML)Q: Is the CASPL1D1/CASPL1D2 negative effect on suberization direct, and does CASPL1D2 modulate PIP2;1 in the suberizing endodermis?
Experiment: Suberin and water-transport phenotyping of caspl1d1/caspl1d2 mutants with cell-type resolution; test CASPL1D2-PIP2;1 functional interaction.
Type: targeted physiology and biochemistry
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