DREB2A is a sequence-specific DNA-binding transcription factor of the AP2/ERF superfamily (ERF/DREB subfamily) in Arabidopsis thaliana. Through its single AP2/ERF DNA-binding domain it recognizes the dehydration-responsive element/C-repeat (DRE/CRT) cis-element (core motif A/GCCGAC, with a preference for ACCGAC) in the promoters of stress-inducible genes and activates their transcription via an acidic C-terminal transactivation domain. It is a nuclear protein and a central regulator of the ABA-independent branch of drought-, high-salinity- and heat-stress-responsive gene expression. DREB2A activity is tightly controlled post-translationally: a central negative regulatory domain (NRD, containing a PEST sequence) targets the protein for ubiquitination by the RING E3 ligases DRIP1 and DRIP2 and degradation by the 26S proteasome under non-stress conditions, while stress (notably heat) stabilizes the protein in the nucleus. A constitutively active form lacking the NRD (DREB2A-CA) confers strong drought and heat tolerance when overexpressed. Under heat stress DREB2A induces the heat-stress regulatory cascade, including the heat-shock transcription factor gene HsfA3, and cooperates with a DPB3-1/NF-YA2/NF-YB3 trimer to selectively activate heat-stress target genes.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0003677 DNA binding | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: Electronic InterPro2GO annotation for the AP2/ERF DNA-binding domain. DREB2A is a bona fide DNA-binding protein, so this is correct, but it is a general parent of the more informative experimentally supported DNA-binding transcription factor activity / DRE-element binding. Reason: Correct but general; subsumed by the specific sequence-specific DNA-binding transcription factor activity and DRE/CRT cis-regulatory region binding annotations. Supporting Evidence: file:ARATH/DREB2A/DREB2A-uniprot.txt DNA_BIND 78..135 |
| GO:0003700 DNA-binding transcription factor activity | IEA GO_REF:0000002 | ACCEPT | Summary: Electronic InterPro2GO annotation of AP2/ERF transcription factor activity, consistent with the experimentally demonstrated function. Reason: DREB2A is an experimentally validated DNA-binding transcription factor; this electronic annotation is corroborated by IDA (PMID:9707537) and ISS annotations to the same term. Supporting Evidence: PMID:9707537 Both the DREB1A and DREB2A proteins specifically bound to the DRE sequence in vitro and activated the transcription of the b-glucuronidase reporter gene driven by the DRE sequence in Arabidopsis leaf protoplasts. |
| GO:0005634 nucleus | IEA GO_REF:0000044 | ACCEPT | Summary: Electronic subcellular-location mapping placing DREB2A in the nucleus, consistent with multiple IDA observations and its role as a transcription factor. Reason: Nuclear localization is experimentally confirmed (GFP-DREB2A in nucleus, N-terminal NLS); the electronic annotation is correct. Supporting Evidence: PMID:16617101 Synthetic green fluorescent protein gave a strong signal in the nucleus under unstressed control conditions when fused to constitutive active DREB2A |
| GO:0006355 regulation of DNA-templated transcription | IEA GO_REF:0000002 | MODIFY | Summary: Electronic InterPro2GO annotation of transcriptional regulation. Correct but general; experimental data specifically support positive regulation of transcription. Reason: Too general: DREB2A is a demonstrated transcriptional activator (reporter transactivation in protoplasts; mapped C-terminal transactivation domain), so the specific term positive regulation of DNA-templated transcription (GO:0045893), already supported on this gene by IDA (PMID:25490919) and IBA, is the appropriate replacement. Proposed replacements: positive regulation of DNA-templated transcription Supporting Evidence: PMID:9707537 activated the transcription of the b-glucuronidase reporter gene driven by the DRE sequence in Arabidopsis leaf protoplasts |
| GO:0005515 protein binding | IPI PMID:18552202 Arabidopsis DREB2A-interacting proteins function as RING E3 ... | MODIFY | Summary: IPI interaction with DRIP1 (Q9M9Y4) / DRIP2 (Q94AY3), RING E3 ubiquitin ligases that ubiquitinate DREB2A and target it for proteasomal degradation. A real, important interaction, but "protein binding" is an uninformative molecular-function term. Reason: Under the GO:0005515 policy the generic term is replaced by the informative MF the paper supports: DRIP1 and DRIP2 are shown to be E3 ubiquitin ligases that bind and ubiquitinate DREB2A, so DREB2A enables ubiquitin protein ligase binding (GO:0031625). The interaction is real and biologically central (post-translational control of DREB2A); it is non-core because it describes how DREB2A is regulated rather than what it does. Proposed replacements: ubiquitin protein ligase binding Supporting Evidence: PMID:18552202 DRIP1 and DRIP2 act as novel negative regulators in drought-responsive gene expression by targeting DREB2A to 26S proteasome proteolysis. PMID:18552202 An in vitro ubiquitination assay showed that they function as E3 ubiquitin ligases and are capable of mediating DREB2A ubiquitination. |
| GO:0005515 protein binding | IPI PMID:19548978 Unequally redundant RCD1 and SRO1 mediate stress and develop... | MODIFY | Summary: IPI interaction with RCD1 (Q8RY59), a hub protein mediating stress and developmental responses. Real interaction but uninformative MF term. Reason: Under the GO:0005515 policy the generic term is replaced by the informative MF. The partner RCD1 is a transcription-factor-binding hub that acts as a coregulator of DREB2A; the same DREB2A-RCD1 interaction carries an EXP transcription coregulator binding (GO:0001221) annotation from PMID:27881680, so the replacement matches the existing curated term rather than inventing a new function. Proposed replacements: transcription coregulator binding Supporting Evidence: PMID:19548978 Unequally redundant RCD1 and SRO1 mediate stress and developmental responses and interact with transcription factors. |
| GO:0005515 protein binding | IPI PMID:21536906 The Arabidopsis thaliana Med25 mediator subunit integrates e... | MODIFY | Summary: IPI interaction with the Mediator subunit MED25 (Q7XYY2-1), consistent with DREB2A recruiting the Mediator complex during transcriptional activation. Real interaction but uninformative MF term. Reason: Under the GO:0005515 policy the generic term is replaced by the informative MF the paper supports. MED25 is a subunit of Mediator, a transcriptional coregulator, and the paper maps the DREB2A region essential for MED25 binding and shows that disrupting it changes DREB2A activator output. Transcription coregulator binding (GO:0001221) captures this. Proposed replacements: transcription coregulator binding Supporting Evidence: PMID:21536906 The Arabidopsis thaliana Med25 mediator subunit integrates environmental cues to control plant development. PMID:21536906 When the interaction between the RD of DREB2A and Mediator is disrupted, either by removal of the RD of DREB2A or by deletion of Med25, then DREB2A functions as an activator |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: IPI interactions from a large-scale phytohormone protein interaction network (DOG1 A0SVK0 and IMPA6 Q9FWY7). Real interactions but uninformative MF term. Reason: Removed under the GO:0005515 policy because the term carries no functional information, not because the interactions are doubted. These are high-throughput interactome hits (DOG1, IMPA6) and the screen does not establish a more informative molecular function for DREB2A, so no replacement is proposed. Supporting Evidence: PMID:32612234 Extensive signal integration by the phytohormone protein network. |
| GO:0005515 protein binding | IPI PMID:22447446 Interactions between DNA, transcriptional regulator Dreb2a a... | MODIFY | Summary: IPI interaction with MED25 (Q7XYY2) characterized structurally (DREB2A disordered region undergoing conformational changes on DNA/MED25 binding; PDB 5OAP). Real interaction but uninformative MF term. Reason: Under the GO:0005515 policy the generic term is replaced by the informative MF the paper supports: direct, biophysically characterized binding of DREB2A to the MED25 subunit of the Mediator coregulatory complex, i.e. transcription coregulator binding (GO:0001221). Proposed replacements: transcription coregulator binding Supporting Evidence: PMID:22447446 Interactions between DNA, transcriptional regulator Dreb2a and the Med25 mediator subunit from Arabidopsis thaliana involve conformational changes. PMID:22447446 Mediator is a multiprotein coregulatory complex that conveys signals from DNA-bound transcriptional regulators to the RNA polymerase II transcription machinery in eukaryotes. |
| GO:0005515 protein binding | IPI PMID:27881680 Structures and Short Linear Motif of Disordered Transcriptio... | MODIFY | Summary: IPI interaction with RCD1 (Q8RY59) via a short linear motif in the DREB2A disordered region binding the RCD1 RST domain. Real interaction but uninformative MF term. Reason: Under the GO:0005515 policy the generic term is replaced by the informative MF. GOA already carries the EXP transcription coregulator binding (GO:0001221) annotation from this same paper for the same DREB2A-RCD1 interaction (reviewed below), so that is the replacement. Proposed replacements: transcription coregulator binding Supporting Evidence: PMID:27881680 Structures and Short Linear Motif of Disordered Transcription Factor Regions Provide Clues to the Interactome of the Cellular Hub Protein Radical-induced Cell Death1. |
| GO:0005515 protein binding | IPI PMID:25490919 Arabidopsis DPB3-1, a DREB2A interactor, specifically enhanc... | MODIFY | Summary: IPI interaction with DPB3-1/NF-YC10 (Q9LN09), the heat-stress-specific coactivator. Real interaction but uninformative MF term. Reason: Under the GO:0005515 policy the generic term is replaced by the informative MF the paper supports. DPB3-1 is identified as a coactivator of DREB2A, and the DPB3-1/NF-YA2/NF-YB3 trimer enhances DREB2A-driven HsfA3 promoter transactivation, so transcription coactivator binding (GO:0001223) is the appropriate replacement. Proposed replacements: transcription coactivator binding Supporting Evidence: PMID:25490919 we identified DNA POLYMERASE II SUBUNIT B3-1 (DPB3-1) PMID:25490919 as a potential coactivator of DREB2A that specifically functions under heat stress |
| GO:0005634 nucleus | IDA PMID:25490919 Arabidopsis DPB3-1, a DREB2A interactor, specifically enhanc... | ACCEPT | Summary: Direct (IDA) nuclear localization of DREB2A, where it interacts with DPB3-1 during heat stress. Core localization. Reason: Experimentally confirmed nuclear localization, consistent with TF function. Supporting Evidence: PMID:25490919 NF-YB3 and DPB3-1 form a trimer with NF-YA2 in the nucleus file:ARATH/DREB2A/DREB2A-deep-research-falcon.md DREB2A functions primarily in the **nucleus** |
| GO:0009408 response to heat | IDA PMID:25490919 Arabidopsis DPB3-1, a DREB2A interactor, specifically enhanc... | ACCEPT | Summary: DREB2A functions in the heat-stress response, cooperating with the DPB3-1/NF-YA2/NF-YB3 trimer to enhance heat-stress-inducible target gene expression (e.g. HsfA3). Core biological process. Reason: Heat-stress role is well established by gain- and loss-of-function and complex-formation data. Supporting Evidence: PMID:25490919 the identified trimer enhances heat stress-inducible gene expression during heat stress responses in cooperation with DREB2A |
| GO:0045893 positive regulation of DNA-templated transcription | IDA PMID:25490919 Arabidopsis DPB3-1, a DREB2A interactor, specifically enhanc... | ACCEPT | Summary: DREB2A positively regulates transcription of stress-inducible target genes; in the heat-stress complex it synergistically activates the HsfA3 promoter. Core function as a transcriptional activator. Reason: DREB2A is a transcriptional activator (UniProt keyword Activator); direct transactivation demonstrated. Supporting Evidence: PMID:25490919 The identified trimer comprising NF-YA2, NF-YB3, and DPB3-1 enhanced HsfA3 promoter transactivation by DREB2A |
| GO:0000976 transcription cis-regulatory region binding | IPI PMID:22037706 Enhanced Y1H assays for Arabidopsis. | ACCEPT | Summary: Enhanced yeast-one-hybrid assay detecting DREB2A binding to target promoter fragments. Supports the sequence-specific DRE/CRT cis-regulatory region binding function. Core molecular activity. Reason: DREB2A binds the DRE/CRT cis-element in promoters; this Y1H-based promoter-binding annotation is consistent with the well-established DNA-binding function. Supporting Evidence: PMID:9707537 Both the DREB1A and DREB2A proteins specifically bound to the DRE sequence in vitro file:ARATH/DREB2A/DREB2A-deep-research-falcon.md DREB2A recognizes **DRE/CRT** elements sharing an **A/GCCGAC core** |
| GO:0000976 transcription cis-regulatory region binding | IPI PMID:25352272 Promoter-based integration in plant defense regulation. | KEEP AS NON CORE | Summary: Promoter cis-regulatory binding detected in a plant-defense regulatory network screen. Supports DRE/CRT cis-element binding activity, though the defense context is a large-scale network node rather than an established in planta DREB2A regulon. Reason: Supports the cis-regulatory binding molecular activity, but the specific defense-network context is peripheral to DREB2A's core abiotic-stress role; redundant with the core GO:0000976 annotation. Supporting Evidence: PMID:25352272 Promoter-based integration in plant defense regulation. |
| GO:0000976 transcription cis-regulatory region binding | IPI PMID:25533953 An Arabidopsis gene regulatory network for secondary cell wa... | KEEP AS NON CORE | Summary: Promoter binding detected in a secondary-cell-wall gene regulatory network screen. Supports cis-regulatory binding activity but in a context peripheral to DREB2A's established abiotic-stress function. Reason: Y1H-network node; consistent with the cis-element binding activity but the secondary-cell-wall context is not an established DREB2A regulon. Supporting Evidence: PMID:25533953 An Arabidopsis gene regulatory network for secondary cell wall synthesis. |
| GO:0000976 transcription cis-regulatory region binding | IPI PMID:27650334 Transcriptional Regulation of Arabidopsis Polycomb Repressiv... | KEEP AS NON CORE | Summary: Promoter binding detected in a study of transcriptional regulation of Polycomb Repressive Complex 2 genes. Supports cis-regulatory binding activity in a peripheral context. Reason: Network-screen binding; consistent with cis-element binding activity but peripheral to DREB2A's core stress role. Supporting Evidence: PMID:27650334 Transcriptional Regulation of Arabidopsis Polycomb Repressive Complex 2 Coordinates Cell-Type Proliferation and Differentiation. |
| GO:0000976 transcription cis-regulatory region binding | IPI PMID:30356219 Transcriptional regulation of nitrogen-associated metabolism... | KEEP AS NON CORE | Summary: Promoter binding detected in a nitrogen-associated metabolism/growth gene regulatory network screen. Supports cis-regulatory binding activity in a peripheral context. Reason: Network-screen binding; consistent with cis-element binding activity but the nitrogen-metabolism context is not an established DREB2A regulon. Supporting Evidence: PMID:30356219 Transcriptional regulation of nitrogen-associated metabolism and growth. |
| GO:0000976 transcription cis-regulatory region binding | IPI PMID:31806676 A PXY-Mediated Transcriptional Network Integrates Signaling ... | KEEP AS NON CORE | Summary: Promoter binding detected in a PXY-mediated vascular-development transcriptional network screen. Supports cis-regulatory binding activity in a peripheral context. Reason: Network-screen binding; consistent with cis-element binding activity but the vascular-development context is not an established DREB2A regulon. Supporting Evidence: PMID:31806676 A PXY-Mediated Transcriptional Network Integrates Signaling Mechanisms to Control Vascular Development in Arabidopsis. |
| GO:0071456 cellular response to hypoxia | HEP PMID:31519798 Integrative Analysis from the Epigenome to Translatome Uncov... | KEEP AS NON CORE | Summary: High-throughput expression/epigenome study in which heat-stress transcripts (a category that includes DREB2A) are progressively upregulated during hypoxia. This is a transcript-level expression association (HEP), not evidence that DREB2A function is required for the hypoxia response. Reason: HEP inference from a high-throughput expression pattern; a transcript association rather than a demonstrated DREB2A function in hypoxia. Retain as non-core rather than remove, as the evidence is a legitimate (if weak) expression-based inference. Supporting Evidence: PMID:31519798 Hypoxia promoted a progressive upregulation of heat stress transcripts |
| GO:0005634 nucleus | ISM GO_REF:0000122 | ACCEPT | Summary: Sequence-based (AtSubP) prediction of nuclear localization, consistent with the multiple experimental IDA observations. Reason: Predicted nuclear localization, corroborated by multiple experimental nuclear IDA annotations. Supporting Evidence: PMID:16617101 Synthetic green fluorescent protein gave a strong signal in the nucleus under unstressed control conditions when fused to constitutive active DREB2A |
| GO:0005634 nucleus | IDA PMID:21443605 A subcellular localization compendium of hydrogen peroxide-i... | ACCEPT | Summary: Direct nuclear localization reported in a subcellular-localization compendium of hydrogen-peroxide-induced proteins. Consistent with TF function. Reason: Experimentally observed nuclear localization, corroborating the core nuclear annotation. Supporting Evidence: PMID:21443605 A subcellular localization compendium of hydrogen peroxide-induced proteins. |
| GO:0005634 nucleus | IDA PMID:18552202 Arabidopsis DREB2A-interacting proteins function as RING E3 ... | ACCEPT | Summary: Direct (IDA) nuclear localization of DREB2A, where it interacts with the RING E3 ligases DRIP1/DRIP2. Core localization. Reason: Experimentally confirmed nuclear localization. Supporting Evidence: PMID:18552202 C3HC4 RING domainβcontaining proteins that interact with the DREB2A protein in the nucleus |
| GO:0010224 response to UV-B | IEP PMID:18266923 Identification of a novel cis-regulatory element for UV-B-in... | KEEP AS NON CORE | Summary: Annotation that DREB2A is a UV-B-responsive gene. The originally cited paper (PMID:18266923) characterizes a novel UV-B cis-element (UVBox) on the ANAC13 promoter and does not itself mention DREB2A, so it is miscited for this annotation; however, DREB2A's UV-B responsiveness is independently supported (HY5-dependent UV-B induction, PMID:14739338). Reason: Correct but peripheral: DREB2A is a UV-B-induced transcript, not an effector that drives the UV-B response; its core role is ABA-independent drought/heat transcriptional regulation. The original GOA reference (PMID:18266923) is miscited (it does not mention DREB2A), so support is re-anchored to PMID:14739338, which documents HY5-dependent UV-B induction of DREB2A. Kept as non-core (consistent with the parallel GO:0010224 IGI annotation) rather than removed, since the underlying UV-B-responsiveness is genuine. Supporting Evidence: PMID:14739338 loss of HY5 impairs the UV-B-responsive expression of several genes |
| GO:0010224 response to UV-B | IGI PMID:14739338 Genome-wide analysis of gene expression reveals function of ... | KEEP AS NON CORE | Summary: DREB2A (At5g05410) is a UV-B-induced transcript whose induction is impaired in the hy5 mutant. This documents DREB2A as a downstream UV-B-responsive gene (genetic dependence on HY5), not that DREB2A function drives the UV-B response. Reason: Expression-readout / genetic-interaction association; DREB2A is a UV-B target gene rather than an effector of the response. Non-core. Supporting Evidence: PMID:14739338 loss of HY5 impairs the UV-B-responsive expression of several genes |
| GO:0010286 heat acclimation | IEP PMID:16807682 Gene expression profiles during heat acclimation in Arabidop... | KEEP AS NON CORE | Summary: Transcript-level induction of DREB2-subfamily genes during heat acclimation in suspension cultures, suggesting cross-talk between heat and dehydration signaling. Expression-based association consistent with the established heat role but not a functional knockout result here. Reason: IEP expression association; biologically consistent with DREB2A's heat role but not the strongest evidence. Retain as non-core. Supporting Evidence: PMID:16807682 of DREB2 (dehydration responsive element-binding factor 2) subfamily genes and |
| GO:0042542 response to hydrogen peroxide | IEP PMID:17030801 Dual function of an Arabidopsis transcription factor DREB2A ... | KEEP AS NON CORE | Summary: The cited reference is the dual drought/heat-function paper; hydrogen peroxide response is not its focus and is not established as a DREB2A function there. Treated as a peripheral expression-based association. Reason: IEP expression-based association lacking strong functional support for an H2O2-specific role; retain as non-core rather than remove. Supporting Evidence: PMID:17030801 Dual function of an Arabidopsis transcription factor DREB2A in water-stress-responsive and heat-stress-responsive gene expression. |
| GO:0009408 response to heat | IMP PMID:17030801 Dual function of an Arabidopsis transcription factor DREB2A ... | ACCEPT | Summary: Loss- and gain-of-function evidence that DREB2A functions in the heat-stress response: DREB2A-CA overexpressors gain thermotolerance and dreb2a knockouts lose it. Core biological process. Reason: Strong genetic (IMP) evidence for a DREB2A role in heat-stress response. Supporting Evidence: PMID:17030801 Thermotolerance was significantly increased in plants overexpressing DREB2A CA and decreased in DREB2A knockout plants. Collectively, these results indicate that DREB2A functions in both water and HS-stress responses. file:ARATH/DREB2A/DREB2A-deep-research-falcon.md DREB2A is also strongly supported as a regulator of heat-responsive gene expression and thermotolerance |
| GO:0003700 DNA-binding transcription factor activity | IDA PMID:9707537 Two transcription factors, DREB1 and DREB2, with an EREBP/AP... | MODIFY | Summary: Direct demonstration that DREB2A binds the DRE sequence and activates DRE-driven reporter transcription. Core molecular function. Reason: DREB2A is an experimentally validated DNA-binding transcription factor, and this paper shows it specifically as an activator (DRE-driven reporter transactivation in leaf protoplasts), consistent with the acidic C-terminal transactivation domain mapped in PMID:16617101. The more specific child term DNA-binding transcription activator activity, RNA polymerase II-specific (GO:0001228) is therefore the better representation of what was shown, and is the MF used in core_functions. Proposed replacements: DNA-binding transcription activator activity, RNA polymerase II-specific Supporting Evidence: PMID:9707537 Both the DREB1A and DREB2A proteins specifically bound to the DRE sequence in vitro and activated the transcription of the b-glucuronidase reporter gene driven by the DRE sequence in Arabidopsis leaf protoplasts. file:ARATH/DREB2A/DREB2A-deep-research-falcon.md DREB2A is a **sequence-specific DNA-binding transcription factor**. |
| GO:0005634 nucleus | IDA PMID:16617101 Functional analysis of an Arabidopsis transcription factor, ... | ACCEPT | Summary: Direct nuclear localization of GFP-DREB2A in Arabidopsis; the protein carries an N-terminal NLS. Core localization. Reason: Experimentally confirmed nuclear localization. Supporting Evidence: PMID:16617101 Synthetic green fluorescent protein gave a strong signal in the nucleus under unstressed control conditions when fused to constitutive active DREB2A |
| GO:0009414 response to water deprivation | IMP PMID:16617101 Functional analysis of an Arabidopsis transcription factor, ... | ACCEPT | Summary: Functional analysis showing DREB2A (via DREB2A-CA gain-of-function) regulates many water-stress-inducible genes and confers drought tolerance. Core biological process. Reason: Well-established role of DREB2A in the drought/water-deprivation response. Supporting Evidence: PMID:16617101 Overexpression of constitutive active DREB2A resulted in significant drought stress tolerance but only slight freezing tolerance in transgenic Arabidopsis plants. file:ARATH/DREB2A/DREB2A-deep-research-falcon.md A primary, experimentally supported role of DREB2A is the **regulation of dehydration/drought-induced gene expression**. |
| GO:0003700 DNA-binding transcription factor activity | ISS PMID:11118137 Arabidopsis transcription factors: genome-wide comparative a... | ACCEPT | Summary: Sequence-similarity-based assignment of DNA-binding transcription factor activity from a genome-wide comparative analysis of Arabidopsis transcription factors. Corroborates the experimentally supported function. Reason: Consistent with the IDA-supported DNA-binding transcription factor activity; correct core molecular function. Supporting Evidence: PMID:9707537 Both the DREB1A and DREB2A proteins specifically bound to the DRE sequence in vitro |
| GO:0009414 response to water deprivation | TAS PMID:10809011 Organization and expression of two Arabidopsis DREB2 genes e... | ACCEPT | Summary: Author-stated role of DREB2 genes as DRE-binding proteins involved in dehydration- and high-salinity-responsive gene expression. Core biological process. Reason: Consistent with the strong IMP evidence for DREB2A in drought/water deprivation response. Supporting Evidence: PMID:10809011 genes are induced by dehydration and high-salt stress. |
| GO:0003700 DNA-binding transcription factor activity | IBA GO_REF:0000033 | ACCEPT | Summary: Phylogenetic (PAINT) inference of DNA-binding transcription factor activity from the DREB subfamily node PTN001261703. DREB2A itself is among the experimentally characterized descendants (AT5G05410 in WITH/FROM), and its own IDA annotation to this term (PMID:9707537) confirms it. Reason: Core molecular function of DREB2A, directly demonstrated by DRE binding and reporter transactivation; the IBA adds that the activity is inherited across the DREB clade. Supporting Evidence: PMID:9707537 Both the DREB1A and DREB2A proteins specifically bound to the DRE sequence in vitro and activated the transcription of the b-glucuronidase reporter gene driven by the DRE sequence in Arabidopsis leaf protoplasts. |
| GO:0000976 transcription cis-regulatory region binding | IBA GO_REF:0000033 | ACCEPT | Summary: Phylogenetic inference of cis-regulatory region binding from the DREB subfamily node PTN001261703. DREB2A binds the DRE/CRT element in stress-inducible promoters, with a preference for ACCGAC. Reason: Consistent with the core molecular function used in core_functions and with the accepted IPI annotation to the same term (PMID:22037706); the target appears among the IBD seeds, marking experimental grounding on DREB2A itself. Supporting Evidence: PMID:16617101 We found that the DREB2A protein could recognize not only A/GCCGACNT, but also A/GCCGACNA/G/C, and prefers ACCGAC to GCCGAC. |
| GO:0005634 nucleus | IBA GO_REF:0000033 | ACCEPT | Summary: Phylogenetic inference that DREB2A acts in the nucleus, consistent with multiple IDA observations of nuclear GFP-DREB2A. Reason: Nuclear localization is experimentally confirmed and is where DREB2A exerts its transcription factor activity. Supporting Evidence: PMID:16617101 Synthetic green fluorescent protein gave a strong signal in the nucleus under unstressed control conditions when fused to constitutive active DREB2A |
| GO:0045893 positive regulation of DNA-templated transcription | IBA GO_REF:0000033 | ACCEPT | Summary: Phylogenetic inference that DREB2A positively regulates transcription. DREB2A carries a C-terminal activation domain and activates DRE-driven target genes. Reason: Matches the IDA annotation to the same term (PMID:25490919) and the mapped transactivation domain. Supporting Evidence: PMID:16617101 DREB2A domain analysis using Arabidopsis protoplasts identified a transcriptional activation domain between residues 254 and 335 |
| GO:0010286 heat acclimation | IBA GO_REF:0000033 | ACCEPT | Summary: Phylogenetic inference of heat acclimation from the DREB2 clade node PTN007858567. DREB2A contributes to acquired thermotolerance by activating heat-stress genes (e.g. HsfA3); dreb2a knockouts lose thermotolerance. Reason: Correct and core: gain- and loss-of-function thermotolerance data on DREB2A itself (PMID:17030801) support it, and DREB2A is one of the IBD seeds. Heat acclimation (synonym thermotolerance) is the more specific child of response to heat (GO:0009408) and describes exactly what the overexpression and knockout phenotypes measure, so it is included in the heat-stress core function. The parallel IEP row (PMID:16807682) stays non-core only because its evidence is expression-level. Supporting Evidence: PMID:17030801 Thermotolerance was significantly increased in plants overexpressing DREB2A CA and decreased in DREB2A knockout plants. |
| GO:0001221 transcription coregulator binding | EXP PMID:27881680 Structures and Short Linear Motif of Disordered Transcriptio... | KEEP AS NON CORE | Summary: DisProt-sourced experimental annotation. A short linear motif in the disordered C-terminal region of DREB2A binds the RST domain of the hub protein RCD1, measured by ITC and NMR; the DREB2A peptide folds into an alpha-helix on binding. Reason: The direct binding is well supported. It is non-core because RCD1 acts mainly as a negative regulator of DREB2A stability rather than as a co-activator in DREB2A's transcriptional output. Supporting Evidence: PMID:27881680 NMR spectroscopy further demonstrated that DREB2A underwent coupled folding and binding with Ξ±-helix formation upon interaction with RCD1 |
| GO:0005515 protein binding | IPI PMID:34473923 Quantification of Conformational Entropy Unravels Effect of ... | REMOVE | Summary: IPI interaction with RCD1 (Q8RY59): a thermodynamic and NMR study of the DREB2A RST-binding motif and its disordered flanking context. Real interaction but uninformative MF term. Reason: Removed under the GO:0005515 policy because the term carries no functional information, not because the interaction is doubted; the RCD1 interaction is real and is captured more informatively by the EXP transcription coregulator binding row (GO:0001221, PMID:27881680). Supporting Evidence: PMID:34473923 inclusion of the disordered context of the DREB2A RST-binding motif induces structuring of the binding motif |
Loading supporting contentβ¦
Download this section (compressed HTML)Q: What is the precise stress-dependent post-translational modification (e.g. phosphorylation of the PEST/NRD region) that stabilizes DREB2A and blocks DRIP1/DRIP2-mediated degradation under drought versus heat stress?
Q: What determines the dehydration- versus heat-specific target-gene selectivity of DREB2A, given that the DPB3-1/NF-Y trimer accounts for only the heat-specific subset?
Experiment: Genome-wide DAP-seq or ChIP-seq with stabilized DREB2A (or DREB2A-CA) under drought and heat conditions to define the direct, condition-specific DRE/CRT regulon and distinguish core targets from large-scale Y1H network hits.
Experiment: Phosphoproteomic mapping of the NRD/PEST region under heat and drought stress combined with phospho-site mutants to test which modifications control DRIP1/DRIP2 recognition and DREB2A stability.
Loading supporting contentβ¦
Download this section (compressed HTML)Loading supporting contentβ¦
Download this section (compressed HTML)Loading supporting contentβ¦
Download this section (compressed HTML)Loading supporting contentβ¦
Download this section (compressed HTML)