DRS1 is an Arabidopsis WDR76/CMR1-related WD40 protein required for normal water retention during drought and for the ABA-dependent reduction of leaf water loss. Two independent drs1 insertion alleles support this role. Its DWD-containing repeat architecture suggests protein-complex interactions, while the biochemical partners linking it to drought physiology and the specificity of any chromatin or DNA binding remain unresolved.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0000792 heterochromatin | IBA GO_REF:0000033 | UNDECIDED | Summary: Human WDR76 associates with heterochromatin-related proteins, but this precise chromatin compartment is not directly resolved for DRS1. The Arabidopsis drought experiments establish a phenotype without chromatin localization. The IBA is a considered family inference, not contradicted by the plant phenotype, yet the specific compartment remains uncertain. Supporting Evidence: PMID:27248496 WDR76 co-localizes to puncta with the heterochromatin proteins CBX1 and CBX5 PMID:20118918 An independent T-DNA allele (Salk_149366C) that we designate as drs1-2 exhibited the same phenotypes in relative water content following drought and ABA-insensitive water transpiration |
| GO:0003677 DNA binding | IBA GO_REF:0000033 | ACCEPT | Summary: The WDR76/CMR1 phylogenetic assignment supports broad DNA binding and is compatible with the conserved DNA-associated repeat family. No target-specific evidence demonstrates loss of broad DNA interaction. Retaining this IBA does not imply recognition of damaged DNA or a particular modified base. Supporting Evidence: PMID:27248496 WDR76 co-localizes to puncta with the heterochromatin proteins CBX1 and CBX5 PMID:20118918 Drs1 is a WD-40 repeat family protein containing a DWD (DDB1 binding WD40) motif |
| GO:0005634 nucleus | IBA GO_REF:0000033 | ACCEPT | Summary: Nuclear localization is a reasonable conserved inference for the WDR76/CMR1 branch, consistent with the independently studied nuclear/chromatin-associated human homolog. The plant drought phenotype neither proves localization nor refutes this broad compartment. Supporting Evidence: PMID:27248496 WDR76 co-localizes to puncta with the heterochromatin proteins CBX1 and CBX5 PMID:20118918 Drs1 is a WD-40 repeat family protein containing a DWD (DDB1 binding WD40) motif |
| GO:0005634 nucleus | ISM GO_REF:0000122 | ACCEPT | Summary: The nuclear localization inference agrees with the WDR76-family context. It is retained without claiming target-specific microscopy or a precise heterochromatin compartment. Supporting Evidence: PMID:27248496 WDR76 co-localizes to puncta with the heterochromatin proteins CBX1 and CBX5 PMID:20118918 Drs1 is a WD-40 repeat family protein containing a DWD (DDB1 binding WD40) motif |
| GO:0009414 response to water deprivation | IMP PMID:20118918 Rational association of genes with traits using a genome-sca... | ACCEPT | Summary: This IMP is directly supported by reduced water retention in two independent DRS1 insertion alleles. The primary paper explicitly names AT1G80710, so the evidence is target-specific and not merely a network-prediction assertion. Supporting Evidence: PMID:20118918 An independent T-DNA allele (Salk_149366C) that we designate as drs1-2 exhibited the same phenotypes in relative water content following drought and ABA-insensitive water transpiration |
| GO:0009737 response to abscisic acid | IMP PMID:20118918 Rational association of genes with traits using a genome-sca... | ACCEPT | Summary: The primary experiment directly shows ABA-insensitive water transpiration in drs1 leaves, with confirmation by a second allele. Retain the ABA response while avoiding an unsupported receptor or general germination mechanism. Supporting Evidence: PMID:20118918 The mutant was insensitive to ABA on water loss whereas the wild type lost significantly less water in the presence of 10 ΞΌM ABA PMID:20118918 An independent T-DNA allele (Salk_149366C) that we designate as drs1-2 exhibited the same phenotypes in relative water content following drought and ABA-insensitive water transpiration |
| GO:0080008 Cul4-RING E3 ubiquitin ligase complex | ISS PMID:18223036 Characterization of Arabidopsis and rice DWD proteins and th... | UNDECIDED | Summary: The DWD motif supports a plausible DDB1-CUL4 substrate-receptor relationship, but the target paper does not demonstrate DRS1 association with a CUL4 complex or identify its substrate. PMID:18223036 tests selected DWD proteins and PRL1; its existence does not establish that DRS1 itself was one of the experimentally confirmed binders. The ISS hypothesis remains plausible but unresolved. Supporting Evidence: PMID:20118918 Drs1 is a WD-40 repeat family protein containing a DWD (DDB1 binding WD40) motif |
| GO:2000001 regulation of DNA damage checkpoint | IBA GO_REF:0000033 | UNDECIDED | Summary: Recruitment of a WDR76 homolog to DNA damage does not by itself establish regulation of the DNA damage checkpoint in Arabidopsis. The DRS1 study does not assay checkpoint signaling, and no target-specific loss of the ancestral role is demonstrated. The precise IBA process remains uncertain. Supporting Evidence: PMID:27248496 WDR76 co-localizes to puncta with the heterochromatin proteins CBX1 and CBX5 PMID:20118918 An independent T-DNA allele (Salk_149366C) that we designate as drs1-2 exhibited the same phenotypes in relative water content following drought and ABA-insensitive water transpiration |
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