EBF2 (EIN3-BINDING F-BOX PROTEIN 2, At5g25350) is a leucine-rich-repeat F-box protein, paralogous to EBF1, that acts as the substrate-recognition subunit of an SCF (SKP1-CUL1-F-box) E3 ubiquitin ligase. SCF(EBF2) binds the ethylene-responsive transcription factors EIN3 and EIL1 and directs their ubiquitination and 26S proteasome-dependent degradation, so negatively regulating ethylene signalling. ebf2 mutants are ethylene-hypersensitive and ebf1 ebf2 double mutants show constitutive ethylene responses and severe growth arrest. Unlike EBF1, EBF2 is transcriptionally induced by ethylene through direct binding of EIN3 to its promoter, forming a negative feedback loop; EBF2 acts mainly in the later phase of the ethylene response and in growth recovery after ethylene removal. Ethylene lowers EBF2 levels by promoting EBF2 protein turnover and by EIN2-directed translational repression mediated by the EBF2 mRNA 3' UTR. EBF2 is nuclear.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005515 protein binding | IPI PMID:14675532 Plant responses to ethylene gas are mediated by SCF(EBF1/EBF... | REMOVE | Summary: The interactor is recorded as SRK2A/SnRK2.4 (At1g10940, P43291), whose synonyms include "ASK1". The EBF papers study binding to the SKP1-like protein ASK1 (SKP1A, At1g75950) during SCF assembly; the SnRK2 kinase is not mentioned in the abstracts, so the interactor identifier may reflect confusion between the two "ASK1" names. Reason: Protein binding is uninformative regardless of partner; removal does not mean an interaction is false. The likely intended SKP1A interaction is covered by the SCF complex and adaptor activity annotations; interactor identity should be checked by the source curators. Supporting Evidence: PMID:14675533 we identify two Arabidopsis F box proteins called EBF1 and EBF2 that interact physically with EIN3/EIL transcription factors |
| GO:0005515 protein binding | IPI PMID:14675532 Plant responses to ethylene gas are mediated by SCF(EBF1/EBF... | MODIFY | Summary: EBF2 binds its ubiquitination substrate EIN3 (PMID:14675532); substrate recognition is the core function of this F-box protein. Reason: Protein binding is uninformative; the substrate-recruiting activity of an SCF F-box subunit is ubiquitin-like ligase-substrate adaptor activity. Proposed replacements: ubiquitin-like ligase-substrate adaptor activity Supporting Evidence: PMID:14675533 we identify two Arabidopsis F box proteins called EBF1 and EBF2 that interact physically with EIN3/EIL transcription factors |
| GO:0005515 protein binding | IPI PMID:14675532 Plant responses to ethylene gas are mediated by SCF(EBF1/EBF... | MODIFY | Summary: EBF2 binds its ubiquitination substrate EIN3 (PMID:14675532); substrate recognition is the core function of this F-box protein. Reason: Protein binding is uninformative; the substrate-recruiting activity of an SCF F-box subunit is ubiquitin-like ligase-substrate adaptor activity. Proposed replacements: ubiquitin-like ligase-substrate adaptor activity Supporting Evidence: PMID:14675533 we identify two Arabidopsis F box proteins called EBF1 and EBF2 that interact physically with EIN3/EIL transcription factors |
| GO:0005515 protein binding | IPI PMID:14675532 Plant responses to ethylene gas are mediated by SCF(EBF1/EBF... | REMOVE | Summary: The interactor is recorded as SRK2A/SnRK2.4 (At1g10940, P43291), whose synonyms include "ASK1". The EBF papers study binding to the SKP1-like protein ASK1 (SKP1A, At1g75950) during SCF assembly; the SnRK2 kinase is not mentioned in the abstracts, so the interactor identifier may reflect confusion between the two "ASK1" names. Reason: Protein binding is uninformative regardless of partner; removal does not mean an interaction is false. The likely intended SKP1A interaction is covered by the SCF complex and adaptor activity annotations; interactor identity should be checked by the source curators. Supporting Evidence: PMID:14675533 we identify two Arabidopsis F box proteins called EBF1 and EBF2 that interact physically with EIN3/EIL transcription factors |
| GO:0005515 protein binding | IPI PMID:14675533 EIN3-dependent regulation of plant ethylene hormone signalin... | MODIFY | Summary: EBF2 binds its ubiquitination substrate EIL1 (PMID:14675533); substrate recognition is the core function of this F-box protein. Reason: Protein binding is uninformative; the substrate-recruiting activity of an SCF F-box subunit is ubiquitin-like ligase-substrate adaptor activity. Proposed replacements: ubiquitin-like ligase-substrate adaptor activity Supporting Evidence: PMID:14675533 we identify two Arabidopsis F box proteins called EBF1 and EBF2 that interact physically with EIN3/EIL transcription factors |
| GO:0005515 protein binding | IPI PMID:14675533 EIN3-dependent regulation of plant ethylene hormone signalin... | MODIFY | Summary: EBF2 binds its ubiquitination substrate EIN3 (PMID:14675533); substrate recognition is the core function of this F-box protein. Reason: Protein binding is uninformative; the substrate-recruiting activity of an SCF F-box subunit is ubiquitin-like ligase-substrate adaptor activity. Proposed replacements: ubiquitin-like ligase-substrate adaptor activity Supporting Evidence: PMID:14675533 we identify two Arabidopsis F box proteins called EBF1 and EBF2 that interact physically with EIN3/EIL transcription factors |
| GO:0005515 protein binding | IPI PMID:14675533 EIN3-dependent regulation of plant ethylene hormone signalin... | REMOVE | Summary: The interactor is recorded as SRK2A/SnRK2.4 (At1g10940, P43291), whose synonyms include "ASK1". The EBF papers study binding to the SKP1-like protein ASK1 (SKP1A, At1g75950) during SCF assembly; the SnRK2 kinase is not mentioned in the abstracts, so the interactor identifier may reflect confusion between the two "ASK1" names. Reason: Protein binding is uninformative regardless of partner; removal does not mean an interaction is false. The likely intended SKP1A interaction is covered by the SCF complex and adaptor activity annotations; interactor identity should be checked by the source curators. Supporting Evidence: PMID:14675533 we identify two Arabidopsis F box proteins called EBF1 and EBF2 that interact physically with EIN3/EIL transcription factors |
| GO:0005515 protein binding | IPI PMID:23166809 A comprehensive analysis of interaction and localization of ... | MODIFY | Summary: EBF2 interacts with the SCF core subunit ASK2 (SKP1B, At5g42190) in a large-scale ASK-F-box interaction study; this is how the F-box protein is assembled into SCF(EBF2). Reason: Protein binding is uninformative; core-subunit binding together with substrate recruitment constitutes F-box adaptor activity (complex membership is captured by GO:0019005). Proposed replacements: ubiquitin-like ligase-substrate adaptor activity Supporting Evidence: PMID:15090654 two F-box proteins, EBF1 and -2, that work coordinately in SCF complexes to repress ethylene action |
| GO:0005634 nucleus | IDA PMID:14675533 EIN3-dependent regulation of plant ethylene hormone signalin... | ACCEPT | Summary: EBF2 is a nuclear protein, consistent with targeting the nuclear transcription factors EIN3/EIL1. Reason: Supported by localization data and function. Supporting Evidence: PMID:14675533 we identify two Arabidopsis F box proteins called EBF1 and EBF2 that interact physically with EIN3/EIL transcription factors |
| GO:0005634 nucleus | IEA GO_REF:0000120 | ACCEPT | Summary: EBF2 is a nuclear protein, consistent with targeting the nuclear transcription factors EIN3/EIL1. Reason: Supported by localization data and function. |
| GO:0005634 nucleus | TAS PMID:14675532 Plant responses to ethylene gas are mediated by SCF(EBF1/EBF... | ACCEPT | Summary: EBF2 is a nuclear protein, consistent with targeting the nuclear transcription factors EIN3/EIL1. Reason: Supported by localization data and function. Supporting Evidence: PMID:14675533 we identify two Arabidopsis F box proteins called EBF1 and EBF2 that interact physically with EIN3/EIL transcription factors |
| GO:0006511 ubiquitin-dependent protein catabolic process | IEA GO_REF:0000117 | ACCEPT | Summary: SCF(EBF1/EBF2) directs ubiquitin/proteasome-dependent degradation of EIN3. Reason: Correct process for this F-box protein. Supporting Evidence: PMID:14675532 In the absence of ethylene, EIN3 is quickly degraded through a ubiquitin/proteasome pathway mediated by two F box proteins, EBF1 and EBF2. |
| GO:0006511 ubiquitin-dependent protein catabolic process | TAS PMID:14675532 Plant responses to ethylene gas are mediated by SCF(EBF1/EBF... | ACCEPT | Summary: SCF(EBF1/EBF2) directs ubiquitin/proteasome-dependent degradation of EIN3. Reason: Correct process for this F-box protein. Supporting Evidence: PMID:14675532 In the absence of ethylene, EIN3 is quickly degraded through a ubiquitin/proteasome pathway mediated by two F box proteins, EBF1 and EBF2. |
| GO:0009507 chloroplast | ISM GO_REF:0000122 | REMOVE | Summary: AtSubP sequence-based chloroplast prediction. EBF2 is a nuclear SCF F-box protein with no transit peptide-dependent function described. Reason: Contradicted by nuclear localization and the SCF/EIN3 mechanism; unsupported prediction. |
| GO:0009723 response to ethylene | IEA GO_REF:0000117 | ACCEPT | Summary: ebf2 mutants are hypersensitive to ethylene; EBF2 overexpression confers insensitivity. Reason: Direct genetic evidence. |
| GO:0009723 response to ethylene | IMP PMID:14675532 Plant responses to ethylene gas are mediated by SCF(EBF1/EBF... | ACCEPT | Summary: ebf2 mutants are hypersensitive to ethylene; EBF2 overexpression confers insensitivity. Reason: Direct genetic evidence. Supporting Evidence: PMID:14675532 Plants containing mutations in either gene show enhanced ethylene response by stabilizing EIN3 |
| GO:0010105 negative regulation of ethylene-activated signaling pathway | IEA GO_REF:0000117 | ACCEPT | Summary: SCF(EBF2) negatively regulates ethylene signalling by degrading EIN3/EIL1, particularly in the late phase and in recovery after ethylene removal; its EIN3-dependent induction forms a negative feedback loop. Reason: Core biological role. Supporting Evidence: PMID:17307926 EBF2 plays a more prominent role during the latter stages of the response and the resumption of growth following ethylene removal |
| GO:0010105 negative regulation of ethylene-activated signaling pathway | TAS PMID:14675532 Plant responses to ethylene gas are mediated by SCF(EBF1/EBF... | ACCEPT | Summary: SCF(EBF2) negatively regulates ethylene signalling by degrading EIN3/EIL1, particularly in the late phase and in recovery after ethylene removal; its EIN3-dependent induction forms a negative feedback loop. Reason: Core biological role. Supporting Evidence: PMID:17307926 EBF2 plays a more prominent role during the latter stages of the response and the resumption of growth following ethylene removal PMID:18466304 EIN3 can bind and activate the EBF2 promoter, indicating that EIN3 modulates EBF2 gene expression in planta |
| GO:0016567 protein ubiquitination | IEA GO_REF:0000041 | ACCEPT | Summary: UniPathway mapping to protein ubiquitination; SCF(EBF2) ubiquitinates EIN3/EIL1. Reason: Correct for an SCF substrate adaptor subunit. Supporting Evidence: PMID:15090654 two F-box proteins, EBF1 and -2, that work coordinately in SCF complexes to repress ethylene action |
| GO:0019005 SCF ubiquitin ligase complex | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference for the LRR F-box clade; EBF2 (At5g25350) is itself a seed. Correct. Reason: EBF2 works in SCF complexes. Supporting Evidence: PMID:15090654 two F-box proteins, EBF1 and -2, that work coordinately in SCF complexes to repress ethylene action |
| GO:0019005 SCF ubiquitin ligase complex | IPI PMID:14675533 EIN3-dependent regulation of plant ethylene hormone signalin... | ACCEPT | Summary: SCF complex membership of EBF2 is well supported. The WITH field names At1g10940 (SRK2A/SnRK2.4), which is probably a confusion with the SKP1-like ASK1 (At1g75950). Reason: The term is correct for EBF2; only the WITH identifier is doubtful and should be checked by the source curators. Supporting Evidence: PMID:15090654 two F-box proteins, EBF1 and -2, that work coordinately in SCF complexes to repress ethylene action |
| GO:0019005 SCF ubiquitin ligase complex | IPI PMID:14675533 EIN3-dependent regulation of plant ethylene hormone signalin... | ACCEPT | Summary: EBF2 associates with CUL1 (At4g02570), placing it in an SCF E3 ligase. Reason: Correct complex membership. Supporting Evidence: PMID:15090654 two F-box proteins, EBF1 and -2, that work coordinately in SCF complexes to repress ethylene action |
| GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT inference; SCF(EBF2) directs proteasomal degradation of EIN3/EIL1. Reason: Correct, specific process. Supporting Evidence: PMID:14675532 In the absence of ethylene, EIN3 is quickly degraded through a ubiquitin/proteasome pathway mediated by two F box proteins, EBF1 and EBF2. PMID:17307926 we show that EIN3 and EIL1 are the main targets of EBF1/2 |
| GO:1990756 ubiquitin-like ligase-substrate adaptor activity | IDA PMID:15090654 Arabidopsis EIN3-binding F-box 1 and 2 form ubiquitin-protei... | NEW | Summary: Proposed informative MF summarizing the protein-binding rows: EBF2 bridges SCF core subunits and the substrates EIN3/EIL1. Reason: Standard MF for a substrate-recruiting F-box subunit, supported by direct interaction and degradation data. Supporting Evidence: PMID:15090654 EBF1 and -2 interact directly with ethylene insensitive 3 (EIN3) PMID:15090654 two F-box proteins, EBF1 and -2, that work coordinately in SCF complexes to repress ethylene action |
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