id: F4JLB7 gene_symbol: RIC7 product_type: PROTEIN status: COMPLETE taxon: id: NCBITaxon:3702 label: Arabidopsis thaliana description: >- F4JLB7 is a 450-residue leucine-rich-repeat receptor-like protein (RLP) encoded by the Arabidopsis thaliana At4g28560 locus. It has an N-terminal signal peptide, an extracellular LRR solenoid, and a C-terminal hydrophobic segment with almost no cytoplasmic tail. That segment could be a single-pass anchor or a GPI-attachment signal. The protein has no protein kinase domain and no CRIB motif. In the PANTHER PTHR48004 tree it is sister to the clade containing TOO MANY MOUTHS (TMM) and RLP29, within a family of noncatalytic cell-surface LRR receptor-like proteins that includes CLAVATA2. Like its relatives it is expected at the plasma membrane and to act in receptor-mediated signaling, but its ligand, receptor-kinase partners and biological role are unknown. Despite sharing the TAIR symbol RIC7, it is not the CRIB-motif ROP effector RIC7, which is encoded by the adjacent locus At4g28556. existing_annotations: - term: id: GO:0005886 label: plasma membrane evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: is_active_in review: summary: >- PAINT places plasma-membrane localization at the root node of PTHR48004 (PTN008274137), with CLV2, TMM, PII-2 and AT4G28560 as seeds. F4JLB7 is inside that family, and its signal peptide, extracellular LRR region and C-terminal hydrophobic anchor fit a cell-surface protein. action: ACCEPT reason: >- Accept the phylogenetic inference. F4JLB7 sits within the family clade that inherited the term (PANTHER 19.0 places it sister to TMM/RLP29), and its own architecture is that of a secretory-pathway, cell-surface LRR protein. One of the four seeds is AT4G28560 itself. That seed may come from the apical-plasma-membrane IDA from PMID:11752391, which this review removes as a wrong-gene attribution. The node is still well grounded by the CLV2, TMM and PII-2 seeds, so the IBA does not depend on the disputed row. supported_by: &id001 - reference_id: file:ARATH/F4JLB7/F4JLB7-uniprot.txt record_section: ["DR:InterPro", "FT:SIGNAL", SQ] supporting_text: |- DR InterPro; IPR001611; Leu-rich_rpt. DR InterPro; IPR032675; LRR_dom_sf. ... FT SIGNAL 1..22 FT /evidence="ECO:0000256|SAM:SignalP" ... GRALYSSARK VLPLIYFPAT LLALYISITQ - reference_id: file:ARATH/F4JLB7/F4JLB7-bioinformatics/RESULTS-rereview.md supporting_text: >- F4JLB7, like TMM, ends in a hydrophobic segment with essentially no cytoplasmic tail. - reference_id: file:ARATH/F4JLB7/F4JLB7-bioinformatics/RESULTS-rereview.md supporting_text: >- In the PANTHER 19.0 tree F4JLB7 is sister to the clade containing TMM (TOO MANY MOUTHS, Q9SSD1) and RLP29 (Q9SJH6), both in subfamily SF42 (node PTN008481374). - term: id: GO:0038023 label: signaling receptor activity evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: enables review: summary: >- PAINT propagated signaling receptor activity from CLV2 at the PTHR48004 root node. F4JLB7 has the noncatalytic extracellular-LRR receptor-like architecture shared by CLV2 and TMM. action: ACCEPT reason: >- Accept the curated node placement. The PAINT curator put this broad MF at the family root, not only on the CLV2 clade, and F4JLB7 descends from that node. Nothing specific to the target argues for loss: the LRR solenoid is intact and the protein is anchored at the cell surface. The curator did not propagate CLV2- or TMM-specific terms such as coreceptor activity, CLE or EPF peptide recognition, or stomatal and meristem processes, and this review treats that omission as deliberate. F4JLB7 is in its own subfamily (SF38), not in SF42 with TMM or SF9 with CLV2. The term is kept at this general level, and the ligand and partner kinases are unresolved. supported_by: - reference_id: file:ARATH/F4JLB7/F4JLB7-bioinformatics/RESULTS-rereview.md supporting_text: >- The nearest experimentally characterised relative is therefore TMM, a paralog in a different subfamily. - reference_id: file:ARATH/F4JLB7/F4JLB7-uniprot.txt record_section: ["DR:InterPro"] supporting_text: |- DR InterPro; IPR001611; Leu-rich_rpt. DR InterPro; IPR032675; LRR_dom_sf. - term: id: GO:0016020 label: membrane evidence_type: IEA original_reference_id: GO_REF:0000044 qualifier: located_in review: summary: >- Membrane localization is correct but general. The UniProt subcellular location behind this mapping is an ARBA prediction ("Single-pass type I membrane protein"). action: MODIFY reason: >- The term is true but less specific than the evidence allows. The signal peptide, extracellular LRR region, C-terminal hydrophobic anchor and the PAINT IBA all point to the plasma membrane, a term the gene already carries. Refine to plasma membrane. Whether the C-terminal segment is a transmembrane anchor or a GPI-attachment signal is unresolved, so no anchoring-specific term is proposed. proposed_replacement_terms: - id: GO:0005886 label: plasma membrane supported_by: *id001 - term: id: GO:0016020 label: membrane evidence_type: TAS original_reference_id: PMID:12068095 qualifier: located_in review: summary: >- This TAIR TAS row cites the Borner et al. 2002 genome-wide prediction of GPI-anchored proteins. The authors predicted that such proteins are targeted to the plasma membrane and cell wall. action: MODIFY reason: >- Membrane is true but general. The cited study frames GPI anchoring as targeting proteins to the plasma membrane, which matches the PAINT IBA and the target architecture. Only the abstract is cached, so this review cannot confirm whether At4g28560 is in the predicted GPI-anchored set. The localization is supported independently, so refine to plasma membrane. proposed_replacement_terms: - id: GO:0005886 label: plasma membrane supported_by: - reference_id: PMID:12068095 supporting_text: >- Glycosylphosphatidylinositol (GPI) anchoring of proteins provides a potential mechanism for targeting to the plant plasma membrane and cell wall. - reference_id: file:ARATH/F4JLB7/F4JLB7-bioinformatics/RESULTS-rereview.md supporting_text: >- This is compatible with either a single-pass type I anchor or a GPI-attachment signal; the analysis cannot distinguish the two. - term: id: GO:0016020 label: membrane evidence_type: TAS original_reference_id: PMID:12805588 qualifier: located_in review: summary: >- This TAIR TAS row cites the Borner et al. 2003 proteomic identification of GPI-anchored proteins. That study recovered four receptor-like proteins from PI-PLC-sensitive fractions. action: MODIFY reason: >- Membrane is true but general. The cached abstract does not name the four receptor-like proteins, so whether F4JLB7 was among the proteomically confirmed GPI-anchored proteins is not verified here. The supported specific localization is the plasma membrane, which the gene already carries by IBA. proposed_replacement_terms: - id: GO:0005886 label: plasma membrane supported_by: - reference_id: PMID:12805588 supporting_text: >- 30 GAPs were identified, including six beta-1,3 glucanases, five phytocyanins, four fasciclin-like arabinogalactan proteins, four receptor-like proteins - reference_id: file:ARATH/F4JLB7/F4JLB7-uniprot.txt record_section: ["FT:SIGNAL"] supporting_text: |- FT SIGNAL 1..22 FT /evidence="ECO:0000256|SAM:SignalP" - term: id: GO:0009507 label: chloroplast evidence_type: ISM original_reference_id: GO_REF:0000122 qualifier: located_in review: summary: >- The chloroplast assignment is a computational AtSubP prediction. It conflicts with the N-terminal signal peptide (residues 1-22), the extracellular-type LRR region, the hydrophobic C terminus and the curated plasma-membrane IBA of the deposited F4JLB7 sequence. action: REMOVE reason: >- This is an unreviewed electronic localization prediction, and it is contradicted by the protein's own architecture. An N-terminal signal peptide directs proteins into the secretory pathway, not to plastids, and the LRR receptor-like organization matches the plasma-membrane localization accepted from the PAINT IBA in this review. No experiment places F4JLB7 in chloroplasts. The RIC7 localization literature concerns the adjacent CRIB locus AT4G28556 and cannot be used for this protein in either direction. Removing an electronic prediction that is contradicted on sequence grounds follows the project's guidance for IEA/ISM inferences. supported_by: *id001 - term: id: GO:0005515 label: protein binding evidence_type: IPI original_reference_id: PMID:11752391 qualifier: enables review: summary: >- The published RIC7 cloning primers in PMID:11752391 match the adjacent AT4G28556 CRIB gene, supporting probable wrong-gene attribution of ROP binding to F4JLB7. action: REMOVE reason: >- This experimental row was assigned to the wrong gene product. A reproducible sequence analysis maps all published RIC7 cloning and RT-PCR primers from this study (and two later RIC7 studies) to the adjacent, oppositely oriented locus AT4G28556, which encodes the 216-residue CRIB protein Q1G3K8. None of the primers matches the AT4G28560 transcript that encodes F4JLB7. Both loci carry the TAIR symbol RIC7, which explains the attribution. The paper's ROP-binding assay concerns a CRIB-motif protein, and the CRIB motif is required for binding GTP-bound Rop1. F4JLB7 has no CRIB motif, only LRRs and a signal peptide, so it cannot be the ROP effector assayed. The interaction partner in the GOA row (AT3G51300, ROP1) fits the CRIB protein Q1G3K8. This removal rests on positive sequence evidence about which locus was assayed, not on the abstract's gene focus. The function itself is not disputed and belongs on Q1G3K8. supported_by: - reference_id: file:ARATH/Q1G3K8/Q1G3K8-bioinformatics/RESULTS.md supporting_text: >- None of the six primers has an exact 3′ match of 14 or more bases in the AT4G28560 transcript NM_118998.1. - reference_id: file:ARATH/Q1G3K8/Q1G3K8-bioinformatics/RESULTS.md supporting_text: >- The comparison strongly assigns these experimental targets to the CRIB locus. - reference_id: PMID:11752391 supporting_text: >- contain a CRIB (for Cdc42/Rac-interactive binding) motif required for their specific interaction with GTP-bound Rop1. additional_reference_ids: - file:projects/PROTNLM_EVALUATION/F4JLB7-RIC7-locus-identity.md source_defects: - defect_type: WRONG_GENE_PRODUCT resource: TAIR slot: DB_Object_ID requested_change: REPOINT asserted_value: UniProtKB:F4JLB7 corrected_value: UniProtKB:Q1G3K8 corrected_label: RIC7 CRIB-motif protein encoded by AT4G28556 cause: SYMBOL_COLLISION confidence: PROBABLE explanation: >- This PMID:11752391 (Wu et al. 2001, RIC7) annotation is attached to F4JLB7 (AT4G28560), an LRR protein without a CRIB motif. The paper's RIC7 is a CRIB-motif ROP effector. All published RIC7 cloning and RT-PCR primers from this and two later RIC7 studies map to the adjacent, oppositely oriented locus AT4G28556 (UniProtKB:Q1G3K8) and to none of the AT4G28560 transcript. Both loci carry the TAIR symbol RIC7. Please check the cloned sequence and, if it confirms this, move the annotation to AT4G28556 / Q1G3K8. supported_by: - reference_id: file:ARATH/Q1G3K8/Q1G3K8-bioinformatics/RESULTS.md supporting_text: >- None of the six primers has an exact 3′ match of 14 or more bases in the AT4G28560 transcript NM_118998.1. - reference_id: PMID:11752391 supporting_text: >- contain a CRIB (for Cdc42/Rac-interactive binding) motif required for their specific interaction with GTP-bound Rop1. - term: id: GO:0009860 label: pollen tube growth evidence_type: IMP original_reference_id: PMID:11752391 qualifier: acts_upstream_of_or_within review: summary: >- The published RIC7 cloning primers in PMID:11752391 match the adjacent AT4G28556 CRIB gene, supporting probable wrong-gene attribution of the pollen-tube overexpression phenotype to F4JLB7. action: REMOVE reason: >- This experimental row was assigned to the wrong gene product. A reproducible sequence analysis maps all published RIC7 cloning and RT-PCR primers from this study (and two later RIC7 studies) to the adjacent, oppositely oriented locus AT4G28556, which encodes the 216-residue CRIB protein Q1G3K8. None of the primers matches the AT4G28560 transcript that encodes F4JLB7. Both loci carry the TAIR symbol RIC7, which explains the attribution. The pollen-tube phenotype comes from overexpressing the cloned RIC7 construct in tobacco pollen, and the primers identify that construct as the CRIB locus. This removal rests on positive sequence evidence about which locus was assayed, not on the abstract's gene focus. The function itself is not disputed and belongs on Q1G3K8. supported_by: - reference_id: file:ARATH/Q1G3K8/Q1G3K8-bioinformatics/RESULTS.md supporting_text: >- None of the six primers has an exact 3′ match of 14 or more bases in the AT4G28560 transcript NM_118998.1. - reference_id: file:ARATH/Q1G3K8/Q1G3K8-bioinformatics/RESULTS.md supporting_text: >- The comparison strongly assigns these experimental targets to the CRIB locus. - reference_id: PMID:11752391 supporting_text: >- contain a CRIB (for Cdc42/Rac-interactive binding) motif required for their specific interaction with GTP-bound Rop1. additional_reference_ids: - file:projects/PROTNLM_EVALUATION/F4JLB7-RIC7-locus-identity.md source_defects: - defect_type: WRONG_GENE_PRODUCT resource: TAIR slot: DB_Object_ID requested_change: REPOINT asserted_value: UniProtKB:F4JLB7 corrected_value: UniProtKB:Q1G3K8 corrected_label: RIC7 CRIB-motif protein encoded by AT4G28556 cause: SYMBOL_COLLISION confidence: PROBABLE explanation: >- This PMID:11752391 (Wu et al. 2001, RIC7) annotation is attached to F4JLB7 (AT4G28560), an LRR protein without a CRIB motif. The paper's RIC7 is a CRIB-motif ROP effector. All published RIC7 cloning and RT-PCR primers from this and two later RIC7 studies map to the adjacent, oppositely oriented locus AT4G28556 (UniProtKB:Q1G3K8) and to none of the AT4G28560 transcript. Both loci carry the TAIR symbol RIC7. Please check the cloned sequence and, if it confirms this, move the annotation to AT4G28556 / Q1G3K8. supported_by: - reference_id: file:ARATH/Q1G3K8/Q1G3K8-bioinformatics/RESULTS.md supporting_text: >- None of the six primers has an exact 3′ match of 14 or more bases in the AT4G28560 transcript NM_118998.1. - reference_id: PMID:11752391 supporting_text: >- contain a CRIB (for Cdc42/Rac-interactive binding) motif required for their specific interaction with GTP-bound Rop1. - term: id: GO:0016324 label: apical plasma membrane evidence_type: IDA original_reference_id: PMID:11752391 qualifier: located_in review: summary: >- The published RIC7 cloning primers in PMID:11752391 match the adjacent AT4G28556 CRIB gene, supporting probable wrong-gene attribution of apical plasma-membrane localization to F4JLB7. action: REMOVE reason: >- This experimental row was assigned to the wrong gene product. A reproducible sequence analysis maps all published RIC7 cloning and RT-PCR primers from this study (and two later RIC7 studies) to the adjacent, oppositely oriented locus AT4G28556, which encodes the 216-residue CRIB protein Q1G3K8. None of the primers matches the AT4G28560 transcript that encodes F4JLB7. Both loci carry the TAIR symbol RIC7, which explains the attribution. The GFP-RIC7 apical localization was imaged with the construct that the primers identify as the CRIB locus. Plasma-membrane association of F4JLB7 is supported separately by the IBA row, but this experiment is not evidence for it. This removal rests on positive sequence evidence about which locus was assayed, not on the abstract's gene focus. The function itself is not disputed and belongs on Q1G3K8. supported_by: - reference_id: file:ARATH/Q1G3K8/Q1G3K8-bioinformatics/RESULTS.md supporting_text: >- None of the six primers has an exact 3′ match of 14 or more bases in the AT4G28560 transcript NM_118998.1. - reference_id: file:ARATH/Q1G3K8/Q1G3K8-bioinformatics/RESULTS.md supporting_text: >- The comparison strongly assigns these experimental targets to the CRIB locus. - reference_id: PMID:11752391 supporting_text: >- contain a CRIB (for Cdc42/Rac-interactive binding) motif required for their specific interaction with GTP-bound Rop1. additional_reference_ids: - file:projects/PROTNLM_EVALUATION/F4JLB7-RIC7-locus-identity.md source_defects: - defect_type: WRONG_GENE_PRODUCT resource: TAIR slot: DB_Object_ID requested_change: REPOINT asserted_value: UniProtKB:F4JLB7 corrected_value: UniProtKB:Q1G3K8 corrected_label: RIC7 CRIB-motif protein encoded by AT4G28556 cause: SYMBOL_COLLISION confidence: PROBABLE explanation: >- This PMID:11752391 (Wu et al. 2001, RIC7) annotation is attached to F4JLB7 (AT4G28560), an LRR protein without a CRIB motif. The paper's RIC7 is a CRIB-motif ROP effector. All published RIC7 cloning and RT-PCR primers from this and two later RIC7 studies map to the adjacent, oppositely oriented locus AT4G28556 (UniProtKB:Q1G3K8) and to none of the AT4G28560 transcript. Both loci carry the TAIR symbol RIC7. Please check the cloned sequence and, if it confirms this, move the annotation to AT4G28556 / Q1G3K8. supported_by: - reference_id: file:ARATH/Q1G3K8/Q1G3K8-bioinformatics/RESULTS.md supporting_text: >- None of the six primers has an exact 3′ match of 14 or more bases in the AT4G28560 transcript NM_118998.1. - reference_id: PMID:11752391 supporting_text: >- contain a CRIB (for Cdc42/Rac-interactive binding) motif required for their specific interaction with GTP-bound Rop1. - term: id: GO:0007165 label: signal transduction evidence_type: IC original_reference_id: PMID:11751054 qualifier: acts_upstream_of_or_within review: summary: >- TIGR made this IC inference in 2003 from a general LRR review, with GO:0005515 as its basis. Broad participation in signal transduction follows from the signaling receptor activity that PAINT assigns to the family. action: ACCEPT reason: >- Accept at this broad level. A signaling receptor takes part in signal transduction by definition, and the IBA receptor-activity row supports that for F4JLB7. The IC's GO:0005515 basis cannot carry the inference: the only protein-binding row for this accession is the ROP1 IPI, which is removed here as a wrong-gene attribution. No specific pathway is known for F4JLB7. This row does not support the ROP2-RIC7-Exo70B1 guard-cell pathway, which belongs to the CRIB protein Q1G3K8, or the stomatal ERECTA/TMM pathway of its paralog TMM. supported_by: - reference_id: file:ARATH/F4JLB7/F4JLB7-bioinformatics/RESULTS-rereview.md supporting_text: >- TMM-specific functions (EPF-peptide recognition with ERECTA-family receptors in stomatal patterning) are not transferable to F4JLB7 on this basis. - reference_id: file:ARATH/F4JLB7/F4JLB7-uniprot.txt record_section: ["DR:InterPro"] supporting_text: |- DR InterPro; IPR001611; Leu-rich_rpt. DR InterPro; IPR032675; LRR_dom_sf. references: - id: GO_REF:0000033 title: Annotation inferences using phylogenetic trees findings: [] - id: GO_REF:0000044 title: >- Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt findings: [] - id: GO_REF:0000122 title: AtSubP analysis findings: [] - id: PMID:11751054 title: The leucine-rich repeat as a protein recognition motif. findings: - statement: Provides general context for leucine-rich repeats as protein recognition modules. - id: PMID:11752391 title: >- A genome-wide analysis of Arabidopsis Rop-interactive CRIB motif-containing proteins that act as Rop GTPase targets. findings: - statement: >- Identifies CRIB-containing Arabidopsis RIC effectors of Rop GTPases. The published RIC7 cloning primers map to AT4G28556, supporting probable misassignment of this experimental evidence to the distinct F4JLB7 LRR protein. - id: PMID:12068095 title: >- Prediction of glycosylphosphatidylinositol-anchored proteins in Arabidopsis. A genomic analysis. findings: - statement: >- Examines Arabidopsis GPI-anchored proteins. The available abstract does not establish whether the exact deposited F4JLB7 product was experimentally tested. - id: PMID:12805588 title: >- Identification of glycosylphosphatidylinositol-anchored proteins in Arabidopsis. A proteomic and genomic analysis. findings: - statement: >- Examines Arabidopsis GPI-anchored proteins. The available abstract does not establish whether the exact deposited F4JLB7 product was experimentally tested. - id: file:ARATH/F4JLB7/F4JLB7-uniprot.txt title: F4JLB7-uniprot.txt - id: file:ARATH/F4JLB7/F4JLB7-hypotheses/prediction-kinase-activity/openscientist.md title: 'OpenScientist investigation: F4JLB7 kinase activity' reference_review: relevance: HIGH correctness: VERIFIED review_notes: >- Re-read the focused kinase-activity adjudication; it directly supports rejecting the ProtNLM2 kinase prediction for this LRR-only receptor-like protein. The 2026-10-20 full re-review was done independently of the ProtNLM2 predictions and does not use this file as support; it is kept for provenance. - id: file:ARATH/Q1G3K8/Q1G3K8-bioinformatics/RESULTS.md title: RIC7 sequence identity and experimental primer mapping reference_review: relevance: HIGH correctness: VERIFIED review_notes: >- Re-read the sequence-identity and primer-mapping report; it directly supports treating published RIC7 assays as targeting AT4G28556/Q1G3K8 rather than AT4G28560/F4JLB7. - id: file:projects/PROTNLM_EVALUATION/F4JLB7-RIC7-locus-identity.md title: F4JLB7 and RIC7 locus identity investigation - id: file:ARATH/F4JLB7/F4JLB7-bioinformatics/RESULTS-rereview.md title: F4JLB7 re-review analyses (PANTHER placement, C-terminal anchor, CRIB scan) findings: - statement: >- In the PANTHER 19.0 tree, F4JLB7 (SF38) is sister to the TMM/RLP29 clade (SF42). It ends in a C-terminal hydrophobic anchor with no cytoplasmic tail, as TMM does, and it has no CRIB motif. A pattern that detects the Q1G3K8 positive control finds none. - id: file:ARATH/F4JLB7/F4JLB7-deep-research-falcon.md title: F4JLB7 deep research (falcon) reference_review: relevance: NONE correctness: MISCITED review_notes: >- The falcon report describes the CRIB-motif ROP2 effector RIC7 (guard-cell stomatal movement, Exo70B1 binding) and asserts that F4JLB7 contains a CRIB domain. That is false for this LRR sequence (see RESULTS-rereview.md). The report concerns the AT4G28556/Q1G3K8 protein, which shares the symbol, and is not used as support for F4JLB7. core_functions: - description: >- Noncatalytic cell-surface LRR receptor-like protein. It is predicted, from PAINT inference at the PTHR48004 family root and from its signal peptide, extracellular LRR solenoid and C-terminal anchor, to act as a signaling receptor at the plasma membrane. Its ligand, partner receptor kinases and biological process are unknown. It lies in its own subfamily, sister to the TMM/RLP29 clade. molecular_function: id: GO:0038023 label: signaling receptor activity locations: - id: GO:0005886 label: plasma membrane supported_by: *id001 suggested_questions: - question: >- Published RIC7 primers match AT4G28556/Q1G3K8 rather than AT4G28560/F4JLB7. At which stage of source curation or AGI-to-UniProt export were the three PMID:11752391 experimental annotations associated with F4JLB7, and what source-level correction preserves the original assay provenance? experts: - TAIR curators - question: >- Is the C-terminal hydrophobic segment of F4JLB7 (residues ~431-450, with no cytoplasmic tail) a transmembrane anchor or a GPI-attachment signal? Was At4g28560 among the four receptor-like proteins recovered from PI-PLC-sensitive fractions by Borner et al. (2003)? experts: - TAIR curators - plant GPI-anchored protein researchers - question: >- Should the UniProt SubName "ROP-interactive CRIB motif-containing protein 7" and the RIC7 gene name on F4JLB7 be revised? F4JLB7 has no CRIB motif and the CRIB-motif RIC7 is encoded by At4g28556 (Q1G3K8). experts: - UniProt curators - TAIR curators suggested_experiments: - description: >- Express a fluorescent-protein fusion of F4JLB7 placed after the signal peptide in Arabidopsis. Test plasma-membrane localization, and test release by phosphatidylinositol-specific phospholipase C to distinguish GPI anchoring from a transmembrane anchor. experiment_type: subcellular localization and PI-PLC sensitivity assay - description: >- Use co-immunoprecipitation or proximity labelling to look for receptor-kinase partners of F4JLB7. Test LRR-RLKs that partner TMM and CLV2 (ERECTA family, CORYNE/CRN). Phenotype at4g28560 loss-of-function lines for stomatal patterning and meristem traits, where its paralogs act. experiment_type: protein interaction and genetic analysis