IAA12 (BODENLOS, BDL; At1g04550) is an Aux/IAA transcriptional repressor of auxin-responsive gene expression. It is a short-lived nuclear protein with an N-terminal domain I carrying an EAR (LxLxL) motif that recruits the TOPLESS co-repressor, a domain II degron that binds the TIR1/AFB auxin receptors in an auxin-dependent manner, and a C-terminal PB1 domain (domains III/IV) through which it heterodimerizes with AUXIN RESPONSE FACTOR proteins, principally the activator ARF5/MONOPTEROS. At low auxin, IAA12 binds MP and recruits TPL to repress MP target genes; auxin promotes IAA12 binding to SCF(TIR1/AFB), ubiquitination and proteasomal degradation, relieving this repressive input to MP-dependent transcription. The bdl gain-of-function mutation in domain II stabilizes IAA12 and blocks MP-dependent specification of the hypophysis and embryonic root meristem, and causes apical-basal patterning and vascular defects; these are suppressed by tpl-1.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0000976 transcription cis-regulatory region binding | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: Regulatory-DNA association is retained with yeast one-hybrid and phylogenetic scope limits. Reason: Publisher Supplementary Table 2 of PMID:25533953 contains the exact IAA12/AT1G04550โAT5G12870 (MYB46 promoter) positive (Sheet1 row445; reproduced in IAA12-source-analysis). Table5 confirms the AUX-IAA clone, and the Methods report repeated screens and sequence confirmation. This is heterologous yeast one-hybrid evidence for regulatory-DNA association, not a demonstrated endogenous MYB46 regulatory function or purified-protein DNA-contact mechanism. The previous explanation that an ARF must tether IAA12 in this assay was not demonstrated and is withdrawn. Absence of a recognized sequence-specific DNA-binding domain does not by itself negate the positive assay. For IAA12, retain the PAINT inherited-node judgment as non-core; the reviewed IAA12 experiment is a family experimental anchor, not a new direct assay on IAA12. No target-specific loss or defective ancestral-node placement has been established. Propagation Review Root cause: NO FAILURE NON CORE Sources checked: PANTHER:PTN001272225 SUPPORTS TRANSFER PAINT ancestral assertion retained; lack of a conventional DNA-binding domain alone is not evidence of lineage-specific loss. Native DNA-contact mechanism remains unresolved. Supporting Evidence: PMID:25533953 All interacting transcription factors were assembled into a cell wall interaction library and the screen was repeated to confirm the results and each clone was sequenced to reconfirm identity. file:ARATH/IAA12/IAA12-source-analysis/README.md PMID:25533953 publisher Supplementary Table 2, Sheet1, row 445 contains transcription factor AT1G04550 (IAA12), promoter AT5G12870 (MYB46), expression fields `ppp, x`, and one reporter activated. |
| GO:0000976 transcription cis-regulatory region binding | IPI PMID:25533953 An Arabidopsis gene regulatory network for secondary cell wa... | KEEP AS NON CORE | Summary: Regulatory-DNA association is retained with yeast one-hybrid and phylogenetic scope limits. Reason: Publisher Supplementary Table 2 of PMID:25533953 contains the exact IAA12/AT1G04550โAT5G12870 (MYB46 promoter) positive (Sheet1 row445; reproduced in IAA12-source-analysis). Table5 confirms the AUX-IAA clone, and the Methods report repeated screens and sequence confirmation. This is heterologous yeast one-hybrid evidence for regulatory-DNA association, not a demonstrated endogenous MYB46 regulatory function or purified-protein DNA-contact mechanism. The previous explanation that an ARF must tether IAA12 in this assay was not demonstrated and is withdrawn. Absence of a recognized sequence-specific DNA-binding domain does not by itself negate the positive assay. Supporting Evidence: PMID:25533953 All interacting transcription factors were assembled into a cell wall interaction library and the screen was repeated to confirm the results and each clone was sequenced to reconfirm identity. file:ARATH/IAA12/IAA12-source-analysis/README.md PMID:25533953 publisher Supplementary Table 2, Sheet1, row 445 contains transcription factor AT1G04550 (IAA12), promoter AT5G12870 (MYB46), expression fields `ppp, x`, and one reporter activated. |
| GO:0003700 DNA-binding transcription factor activity | ISS PMID:11118137 Arabidopsis transcription factors: genome-wide comparative a... | MODIFY | Summary: Refine the historical transcription-factor classification to corepressor activity. Reason: PMID:11118137 is a genome-wide regulator classification, not a IAA12-specific DNA-binding/transactivation assay. The specific functional evidence supports repression through ARF association. Refine to transcription corepressor activity. The separately retained promoter-association term does not by itself establish an autonomous DNA-binding transcription-factor activity. Proposed replacements: transcription corepressor activity Supporting Evidence: PMID:18258861 TPL can repress transcription in vivo and is required for IAA12/BDL repressive activity |
| GO:0005515 protein binding | IPI PMID:12101120 The Arabidopsis BODENLOS gene encodes an auxin response prot... | MODIFY | Summary: Refine the independently demonstrated ARF interaction to transcription-factor binding. Reason: This row preserves the original PMID:12101120 and partner UniProtKB:P93024. Independent primary PMID:12101120 directly establishes this same IAA12โARF partnership, so the function is more informative as DNA-binding transcription factor binding. The partner-specific interaction is not generalized to an obligatory native stoichiometry, every tissue, or all Aux/IAA/ARF pairs. Proposed replacements: DNA-binding transcription factor binding Supporting Evidence: PMID:12101120 BODENLOS and MONOPTEROS interact in the yeast two-hybrid assay |
| GO:0005515 protein binding | IPI PMID:21734647 The auxin signalling network translates dynamic input into r... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:O24407 interaction record. Reason: PMID:21734647 is the systematic Aux/IAAโARF yeast two-hybrid network, with selected BiFC validations. This row identifies UniProtKB:O24407. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:21734647 In this network, the vast majority of the Aux/IAAs interact with all the ARF activators |
| GO:0005515 protein binding | IPI PMID:21734647 The auxin signalling network translates dynamic input into r... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:O24408 interaction record. Reason: PMID:21734647 is the systematic Aux/IAAโARF yeast two-hybrid network, with selected BiFC validations. This row identifies UniProtKB:O24408. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:21734647 In this network, the vast majority of the Aux/IAAs interact with all the ARF activators |
| GO:0005515 protein binding | IPI PMID:21734647 The auxin signalling network translates dynamic input into r... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:O24409 interaction record. Reason: PMID:21734647 is the systematic Aux/IAAโARF yeast two-hybrid network, with selected BiFC validations. This row identifies UniProtKB:O24409. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:21734647 In this network, the vast majority of the Aux/IAAs interact with all the ARF activators |
| GO:0005515 protein binding | IPI PMID:21734647 The auxin signalling network translates dynamic input into r... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:O24410 interaction record. Reason: PMID:21734647 is the systematic Aux/IAAโARF yeast two-hybrid network, with selected BiFC validations. This row identifies UniProtKB:O24410. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:21734647 In this network, the vast majority of the Aux/IAAs interact with all the ARF activators |
| GO:0005515 protein binding | IPI PMID:21734647 The auxin signalling network translates dynamic input into r... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:P33077 interaction record. Reason: PMID:21734647 is the systematic Aux/IAAโARF yeast two-hybrid network, with selected BiFC validations. This row identifies UniProtKB:P33077. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:21734647 In this network, the vast majority of the Aux/IAAs interact with all the ARF activators |
| GO:0005515 protein binding | IPI PMID:21734647 The auxin signalling network translates dynamic input into r... | MODIFY | Summary: Refine the independently demonstrated ARF interaction to transcription-factor binding. Reason: This row preserves the original PMID:21734647 and partner UniProtKB:P93024. Independent primary PMID:12101120 directly establishes this same IAA12โARF partnership, so the function is more informative as DNA-binding transcription factor binding. The partner-specific interaction is not generalized to an obligatory native stoichiometry, every tissue, or all Aux/IAA/ARF pairs. Proposed replacements: DNA-binding transcription factor binding Supporting Evidence: PMID:12101120 BODENLOS and MONOPTEROS interact in the yeast two-hybrid assay |
| GO:0005515 protein binding | IPI PMID:21734647 The auxin signalling network translates dynamic input into r... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:P93830 interaction record. Reason: PMID:21734647 is the systematic Aux/IAAโARF yeast two-hybrid network, with selected BiFC validations. This row identifies UniProtKB:P93830. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:21734647 In this network, the vast majority of the Aux/IAAs interact with all the ARF activators |
| GO:0005515 protein binding | IPI PMID:21734647 The auxin signalling network translates dynamic input into r... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:Q38822 interaction record. Reason: PMID:21734647 is the systematic Aux/IAAโARF yeast two-hybrid network, with selected BiFC validations. This row identifies UniProtKB:Q38822. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:21734647 In this network, the vast majority of the Aux/IAAs interact with all the ARF activators |
| GO:0005515 protein binding | IPI PMID:21734647 The auxin signalling network translates dynamic input into r... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:Q38825 interaction record. Reason: PMID:21734647 is the systematic Aux/IAAโARF yeast two-hybrid network, with selected BiFC validations. This row identifies UniProtKB:Q38825. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:21734647 In this network, the vast majority of the Aux/IAAs interact with all the ARF activators |
| GO:0005515 protein binding | IPI PMID:21734647 The auxin signalling network translates dynamic input into r... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:Q38826 interaction record. Reason: PMID:21734647 is the systematic Aux/IAAโARF yeast two-hybrid network, with selected BiFC validations. This row identifies UniProtKB:Q38826. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:21734647 In this network, the vast majority of the Aux/IAAs interact with all the ARF activators |
| GO:0005515 protein binding | IPI PMID:21734647 The auxin signalling network translates dynamic input into r... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:Q38831 interaction record. Reason: PMID:21734647 is the systematic Aux/IAAโARF yeast two-hybrid network, with selected BiFC validations. This row identifies UniProtKB:Q38831. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:21734647 In this network, the vast majority of the Aux/IAAs interact with all the ARF activators |
| GO:0005515 protein binding | IPI PMID:21734647 The auxin signalling network translates dynamic input into r... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:Q38832 interaction record. Reason: PMID:21734647 is the systematic Aux/IAAโARF yeast two-hybrid network, with selected BiFC validations. This row identifies UniProtKB:Q38832. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:21734647 In this network, the vast majority of the Aux/IAAs interact with all the ARF activators |
| GO:0005515 protein binding | IPI PMID:21734647 The auxin signalling network translates dynamic input into r... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:Q8H174 interaction record. Reason: PMID:21734647 is the systematic Aux/IAAโARF yeast two-hybrid network, with selected BiFC validations. This row identifies UniProtKB:Q8H174. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:21734647 In this network, the vast majority of the Aux/IAAs interact with all the ARF activators |
| GO:0005515 protein binding | IPI PMID:21734647 The auxin signalling network translates dynamic input into r... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:Q9XFM0 interaction record. Reason: PMID:21734647 is the systematic Aux/IAAโARF yeast two-hybrid network, with selected BiFC validations. This row identifies UniProtKB:Q9XFM0. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:21734647 In this network, the vast majority of the Aux/IAAs interact with all the ARF activators |
| GO:0005515 protein binding | IPI PMID:22096563 Large-scale protein-protein interaction analysis in Arabidop... | MODIFY | Summary: Refine the independently demonstrated ARF interaction to transcription-factor binding. Reason: This row preserves the original PMID:22096563 and partner UniProtKB:P93024. Independent primary PMID:12101120 directly establishes this same IAA12โARF partnership, so the function is more informative as DNA-binding transcription factor binding. The partner-specific interaction is not generalized to an obligatory native stoichiometry, every tissue, or all Aux/IAA/ARF pairs. Proposed replacements: DNA-binding transcription factor binding Supporting Evidence: PMID:12101120 BODENLOS and MONOPTEROS interact in the yeast two-hybrid assay |
| GO:0005515 protein binding | IPI PMID:22096563 Large-scale protein-protein interaction analysis in Arabidop... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:Q9C5W9 interaction record. Reason: PMID:22096563 is the Arabidopsis mesophyll protoplast split-luciferase study using ARF C-terminal domains. This row identifies UniProtKB:Q9C5W9. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:22096563 we further applied the SFLC assay to testing 132 binary PPIs among 8 auxin response factors (ARFs) and 12 Aux/IAA proteins |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:A0A2H1ZEF6 interaction record. Reason: PMID:32612234 is the phytohormone binary-interaction network; cached abstract describes the screen but not each pair-specific assay. This row identifies UniProtKB:A0A2H1ZEF6. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:O22179 interaction record. Reason: PMID:32612234 is the phytohormone binary-interaction network; cached abstract describes the screen but not each pair-specific assay. This row identifies UniProtKB:O22179. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:O24407 interaction record. Reason: PMID:32612234 is the phytohormone binary-interaction network; cached abstract describes the screen but not each pair-specific assay. This row identifies UniProtKB:O24407. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:O24408 interaction record. Reason: PMID:32612234 is the phytohormone binary-interaction network; cached abstract describes the screen but not each pair-specific assay. This row identifies UniProtKB:O24408. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:O24409 interaction record. Reason: PMID:32612234 is the phytohormone binary-interaction network; cached abstract describes the screen but not each pair-specific assay. This row identifies UniProtKB:O24409. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:O24410 interaction record. Reason: PMID:32612234 is the phytohormone binary-interaction network; cached abstract describes the screen but not each pair-specific assay. This row identifies UniProtKB:O24410. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:P33077 interaction record. Reason: PMID:32612234 is the phytohormone binary-interaction network; cached abstract describes the screen but not each pair-specific assay. This row identifies UniProtKB:P33077. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:P49677 interaction record. Reason: PMID:32612234 is the phytohormone binary-interaction network; cached abstract describes the screen but not each pair-specific assay. This row identifies UniProtKB:P49677. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:P49678 interaction record. Reason: PMID:32612234 is the phytohormone binary-interaction network; cached abstract describes the screen but not each pair-specific assay. This row identifies UniProtKB:P49678. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:P93830 interaction record. Reason: PMID:32612234 is the phytohormone binary-interaction network; cached abstract describes the screen but not each pair-specific assay. This row identifies UniProtKB:P93830. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:Q38822 interaction record. Reason: PMID:32612234 is the phytohormone binary-interaction network; cached abstract describes the screen but not each pair-specific assay. This row identifies UniProtKB:Q38822. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:Q38824 interaction record. Reason: PMID:32612234 is the phytohormone binary-interaction network; cached abstract describes the screen but not each pair-specific assay. This row identifies UniProtKB:Q38824. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:Q38825 interaction record. Reason: PMID:32612234 is the phytohormone binary-interaction network; cached abstract describes the screen but not each pair-specific assay. This row identifies UniProtKB:Q38825. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:Q38826 interaction record. Reason: PMID:32612234 is the phytohormone binary-interaction network; cached abstract describes the screen but not each pair-specific assay. This row identifies UniProtKB:Q38826. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:Q38828 interaction record. Reason: PMID:32612234 is the phytohormone binary-interaction network; cached abstract describes the screen but not each pair-specific assay. This row identifies UniProtKB:Q38828. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:Q38831 interaction record. Reason: PMID:32612234 is the phytohormone binary-interaction network; cached abstract describes the screen but not each pair-specific assay. This row identifies UniProtKB:Q38831. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:Q38832 interaction record. Reason: PMID:32612234 is the phytohormone binary-interaction network; cached abstract describes the screen but not each pair-specific assay. This row identifies UniProtKB:Q38832. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:Q67XT7 interaction record. Reason: PMID:32612234 is the phytohormone binary-interaction network; cached abstract describes the screen but not each pair-specific assay. This row identifies UniProtKB:Q67XT7. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:Q8H174 interaction record. Reason: PMID:32612234 is the phytohormone binary-interaction network; cached abstract describes the screen but not each pair-specific assay. This row identifies UniProtKB:Q8H174. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:Q8L9Y3 interaction record. Reason: PMID:32612234 is the phytohormone binary-interaction network; cached abstract describes the screen but not each pair-specific assay. This row identifies UniProtKB:Q8L9Y3. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:Q8LAL2 interaction record. Reason: PMID:32612234 is the phytohormone binary-interaction network; cached abstract describes the screen but not each pair-specific assay. This row identifies UniProtKB:Q8LAL2. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:Q9C5W9 interaction record. Reason: PMID:32612234 is the phytohormone binary-interaction network; cached abstract describes the screen but not each pair-specific assay. This row identifies UniProtKB:Q9C5W9. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:Q9FEE2 interaction record. Reason: PMID:32612234 is the phytohormone binary-interaction network; cached abstract describes the screen but not each pair-specific assay. This row identifies UniProtKB:Q9FEE2. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:Q9FJJ3 interaction record. Reason: PMID:32612234 is the phytohormone binary-interaction network; cached abstract describes the screen but not each pair-specific assay. This row identifies UniProtKB:Q9FJJ3. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:Q9FWY7 interaction record. Reason: PMID:32612234 is the phytohormone binary-interaction network; cached abstract describes the screen but not each pair-specific assay. This row identifies UniProtKB:Q9FWY7. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:Q9XFM0 interaction record. Reason: PMID:32612234 is the phytohormone binary-interaction network; cached abstract describes the screen but not each pair-specific assay. This row identifies UniProtKB:Q9XFM0. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Policy removal of bare protein binding; retain the IAA12โUniProtKB:Q9ZSY8 interaction record. Reason: PMID:32612234 is the phytohormone binary-interaction network; cached abstract describes the screen but not each pair-specific assay. This row identifies UniProtKB:Q9ZSY8. The measured interaction is not declared false. Bare GO:0005515 does not specify the regulatory function, so it is removed under the repository protein-binding exception. A physical interaction alone does not establish an endogenous obligatory complex, a partner-specific physiological output, or a new biological process. Pair provenance remains in this reason and the unchanged source fields. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0005634 nucleus | IBA GO_REF:0000033 | ACCEPT | Summary: Retain nuclear localization with the actual assay or inference scope. Reason: Nuclear assignment is consistent with the curated ancestral/domain inference and the demonstrated TPL-dependent repression of ARF5-controlled transcription. This support is functional/localization inference rather than a new microscopy claim. No target-specific cytoplasmic-only localization is known from the inspected sources; retain the nucleus term. Supporting Evidence: PMID:18258861 TPL can repress transcription in vivo and is required for IAA12/BDL repressive activity PMID:12101120 BODENLOS and MONOPTEROS interact in the yeast two-hybrid assay |
| GO:0005634 nucleus | IEA GO_REF:0000120 | ACCEPT | Summary: Retain nuclear localization with the actual assay or inference scope. Reason: Nuclear assignment is consistent with the curated ancestral/domain inference and the demonstrated TPL-dependent repression of ARF5-controlled transcription. This support is functional/localization inference rather than a new microscopy claim. No target-specific cytoplasmic-only localization is known from the inspected sources; retain the nucleus term. Supporting Evidence: PMID:18258861 TPL can repress transcription in vivo and is required for IAA12/BDL repressive activity PMID:12101120 BODENLOS and MONOPTEROS interact in the yeast two-hybrid assay |
| GO:0005634 nucleus | ISM GO_REF:0000122 | ACCEPT | Summary: Retain nuclear localization with the actual assay or inference scope. Reason: Nuclear assignment is consistent with the curated ancestral/domain inference and the demonstrated TPL-dependent repression of ARF5-controlled transcription. This support is functional/localization inference rather than a new microscopy claim. No target-specific cytoplasmic-only localization is known from the inspected sources; retain the nucleus term. Supporting Evidence: PMID:18258861 TPL can repress transcription in vivo and is required for IAA12/BDL repressive activity PMID:12101120 BODENLOS and MONOPTEROS interact in the yeast two-hybrid assay |
| GO:0006355 regulation of DNA-templated transcription | IBA GO_REF:0000033 | MODIFY | Summary: Refine transcriptional regulation to the demonstrated repressive direction. Reason: IAA12 functions as an Aux/IAA repressor of ARF-dependent transcription; negative regulation is the supported specific process. For IAA12 and IAA17 it is already represented elsewhere in this review; refinement does not manufacture new coverage. IAA19 assignment combines its direct ARF7 interaction, dominant repressor phenotypes and feedback experiments, rather than treating auxin-induced expression alone as participation. Propagation Review Root cause: TERM SCOPING PROBLEM Failure modes: GRANULARITY MISMATCH Sources checked: PANTHER:PTN001272225 SUPPORTS TRANSFER The inherited broad claim is retained as true; target-specific repression/signaling evidence supports a narrower term. No ancestral-node error, loss or donor circularity is asserted. Proposed replacements: negative regulation of DNA-templated transcription Supporting Evidence: PMID:18258861 TPL can repress transcription in vivo and is required for IAA12/BDL repressive activity |
| GO:0006355 regulation of DNA-templated transcription | IEA GO_REF:0000002 | MODIFY | Summary: Refine transcriptional regulation to the demonstrated repressive direction. Reason: IAA12 functions as an Aux/IAA repressor of ARF-dependent transcription; negative regulation is the supported specific process. For IAA12 and IAA17 it is already represented elsewhere in this review; refinement does not manufacture new coverage. IAA19 assignment combines its direct ARF7 interaction, dominant repressor phenotypes and feedback experiments, rather than treating auxin-induced expression alone as participation. Proposed replacements: negative regulation of DNA-templated transcription Supporting Evidence: PMID:18258861 TPL can repress transcription in vivo and is required for IAA12/BDL repressive activity |
| GO:0009880 embryonic pattern specification | IMP PMID:10068632 The auxin-insensitive bodenlos mutation affects primary root... | KEEP AS NON CORE | Summary: Retain the native developmental role with dominant-repressor and tissue context. Reason: PMID:10068632 supports the Arabidopsis embryonic root/vascular-patterning context. Stabilized bdl is a gain-of-function repressor, not a null; its phenotype must not be read as the normal protein positively driving every developmental output. The interaction/repression mechanism places IAA12 in transcriptional control of this process, while the developmental term is peripheral to its core molecular role. No transfer of Arabidopsis root anatomy to liverworts is implied. Supporting Evidence: PMID:10068632 BDL is involved in auxin-mediated processes of apical-basal patterning in the Arabidopsis embryo |
| GO:0010051 xylem and phloem pattern formation | IMP PMID:18258861 TOPLESS mediates auxin-dependent transcriptional repression ... | KEEP AS NON CORE | Summary: Retain the native developmental role with dominant-repressor and tissue context. Reason: PMID:18258861 supports the Arabidopsis embryonic root/vascular-patterning context. Stabilized bdl is a gain-of-function repressor, not a null; its phenotype must not be read as the normal protein positively driving every developmental output. The interaction/repression mechanism places IAA12 in transcriptional control of this process, while the developmental term is peripheral to its core molecular role. No transfer of Arabidopsis root anatomy to liverworts is implied. Supporting Evidence: PMID:18258861 TPL can repress transcription in vivo and is required for IAA12/BDL repressive activity PMID:18258861 tpl-1 can suppress the patterning defects of the bdl-1 mutant |
| GO:0042802 identical protein binding | IPI PMID:12101120 The Arabidopsis BODENLOS gene encodes an auxin response prot... | KEEP AS NON CORE | Summary: Retain experimentally annotated self-association without requiring a universal homodimer. Reason: PMID:12101120 supplies the original IAA12 self-interaction assertion. Aux/IAA PB1-mediated oligomerization is compatible with this observation, but identical-protein binding is not the main transcriptional activity and does not establish an obligatory native stoichiometry. For network sources, retain the curated pair-level assertion with heterologous assay limits; no unmeasured in-planta self-complex is inferred. Supporting Evidence: PMID:12101120 BODENLOS and MONOPTEROS interact in the yeast two-hybrid assay |
| GO:0042802 identical protein binding | IPI PMID:21734647 The auxin signalling network translates dynamic input into r... | KEEP AS NON CORE | Summary: Retain experimentally annotated self-association without requiring a universal homodimer. Reason: PMID:21734647 supplies the original IAA12 self-interaction assertion. Aux/IAA PB1-mediated oligomerization is compatible with this observation, but identical-protein binding is not the main transcriptional activity and does not establish an obligatory native stoichiometry. For network sources, retain the curated pair-level assertion with heterologous assay limits; no unmeasured in-planta self-complex is inferred. Supporting Evidence: PMID:21734647 In this network, the vast majority of the Aux/IAAs interact with all the ARF activators |
| GO:0042802 identical protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | KEEP AS NON CORE | Summary: Retain experimentally annotated self-association without requiring a universal homodimer. Reason: PMID:32612234 supplies the original IAA12 self-interaction assertion. Aux/IAA PB1-mediated oligomerization is compatible with this observation, but identical-protein binding is not the main transcriptional activity and does not establish an obligatory native stoichiometry. For network sources, retain the curated pair-level assertion with heterologous assay limits; no unmeasured in-planta self-complex is inferred. Supporting Evidence: PMID:32612234 a systems-level map of the Arabidopsis phytohormone signalling network, consisting of more than 2,000 binary protein-protein interactions |
| GO:0045892 negative regulation of DNA-templated transcription | IDA PMID:18258861 TOPLESS mediates auxin-dependent transcriptional repression ... | ACCEPT | Summary: IAA12 participates directly in transcriptional repression through TPL recruitment. Reason: The original primary identifies the EAR-dependent IAA12โTPL interaction and requires TPL for IAA12 repression. This is direct regulatory participation, not an inference from IAA12 degradation or gene expression alone. Supporting Evidence: PMID:18258861 TPL can repress transcription in vivo and is required for IAA12/BDL repressive activity |
| GO:0048364 root development | IMP PMID:18258861 TOPLESS mediates auxin-dependent transcriptional repression ... | KEEP AS NON CORE | Summary: Retain the native developmental role with dominant-repressor and tissue context. Reason: PMID:18258861 supports the Arabidopsis embryonic root/vascular-patterning context. Stabilized bdl is a gain-of-function repressor, not a null; its phenotype must not be read as the normal protein positively driving every developmental output. The interaction/repression mechanism places IAA12 in transcriptional control of this process, while the developmental term is peripheral to its core molecular role. No transfer of Arabidopsis root anatomy to liverworts is implied. Supporting Evidence: PMID:18258861 TPL can repress transcription in vivo and is required for IAA12/BDL repressive activity PMID:18258861 tpl-1 can suppress the patterning defects of the bdl-1 mutant |
| GO:0009734 auxin-activated signaling pathway | IMP PMID:15992545 Plant development is regulated by a family of auxin receptor... | NEW | Summary: NEW annotation. IAA12/BDL is an Aux/IAA co-receptor/substrate of the TIR1/AFB auxin receptors: all TIR1/AFB proteins bind BDL, BDL is stabilized when the receptors are lost, and stabilized bdl blocks MP-dependent auxin responses. IAA12 does part of the work of the pathway (its degron completes the auxin-binding co-receptor and its degradation is the signalling step), so this is participation rather than mere requirement. Reason: Aux/IAA proteins are integral components of the nuclear auxin signalling pathway; the existing GOA set lacks this process for IAA12. This is an inherited NEW proposal, preserved from the baseline rather than added in this audit. IAA12 performs the repressive signaling step and binds the substrate-recognition receptor; being degraded does not itself justify a protein-catabolic-process annotation. Supporting Evidence: PMID:15992545 all TIR1/AFB proteins interact with BDL, and BDL is stabilized in triple mutant plants PMID:22466420 IAA12 interacted specifically with TIR1 and AFB2 at 100 ฮผM IAA PMID:22466420 TIR1 and the Aux/IAA are both necessary and sufficient for auxin binding and act as auxin co-receptors |
| GO:0031625 ubiquitin protein ligase binding | IPI PMID:15992545 Plant development is regulated by a family of auxin receptor... | NEW | Summary: NEW annotation. All four TIR1/AFB F-box auxin receptors bind BDL/IAA12, and IAA12 binds TIR1 and AFB2 in an auxin-dependent manner in yeast; BDL is stabilized in receptor mutants. IAA12 binding SCF(TIR1/AFB) as its ubiquitination substrate is captured by ubiquitin protein ligase binding (as for IAA17). Reason: Informative replacement for the receptor interaction, which is absent from GOA for IAA12. This is an inherited NEW proposal, preserved from the baseline rather than added in this audit. IAA12 performs the repressive signaling step and binds the substrate-recognition receptor; being degraded does not itself justify a protein-catabolic-process annotation. Supporting Evidence: PMID:15992545 all TIR1/AFB proteins interact with BDL, and BDL is stabilized in triple mutant plants PMID:22466420 IAA12 interacted specifically with TIR1 and AFB2 at 100 ฮผM IAA |
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Download this section (compressed HTML)Q: Should Aux/IAA proteins carry contributes_to auxin receptor activity / auxin binding, given that the TIR1/AFB-Aux/IAA pair forms the high-affinity auxin co-receptor (Calderon Villalobos et al. 2012)?
Q: What determines the preference of BDL/IAA12 for MP/ARF5 over other activator ARFs in the embryo, and how much is due to co-expression versus PB1 affinity?
Q: Does Aux/IAA regulatory-DNA association observed in yeast one-hybrid assays involve direct sequence-specific DNA contacts or an unidentified bridging component in that assay, and what is its native significance?
Experiment: Measure IAA12 degron affinity for each TIR1/AFB receptor in purified co-receptor assays across auxin concentrations.
Hypothesis: IAA12 is a relatively low-affinity co-receptor substrate, requiring high auxin levels for degradation, which shapes MP activity in the embryo.
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