MYC2/JIN1/rd22BP1 is an Arabidopsis nuclear basic helix-loop-helix transcription factor that binds cis-regulatory DNA motifs and controls jasmonate-responsive transcription. JAZ repressors restrain MYC2 activity until jasmonate signaling promotes their degradation. MYC2 coordinates defense, secondary metabolism and growth, including insect resistance, tryptophan-derived and flavonoid metabolism, and root development. Its activity is also modulated by ABA and ethylene signaling partners. MYC2 forms homotetramers that enhance DNA binding and transcriptional regulation.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0009867 jasmonic acid mediated signaling pathway | IC PMID:17637675 The JAZ family of repressors is the missing link in jasmonat... | NEW | Summary: MYC2 functions as a JAZ-regulated transcriptional effector of jasmonate signaling. Reason: MYC2 is a transcriptional effector within the COI1-JAZ jasmonate pathway, not merely a downstream incidental response. Primary studies establish JAZ regulation of MYC2 and MYC2-dependent jasmonate transcription. Supporting Evidence: PMID:17637675 Moreover, JAI3 negatively regulates the key transcriptional activator of jasmonate responses, MYC2. PMID:21321051 MYC2, MYC3, and MYC4 were all capable of inducing expression of JAZ::GUS reporter constructs following transfection of carrot protoplasts. |
| GO:0006568 L-tryptophan metabolic process | IEP PMID:17616737 MYC2 differentially modulates diverse jasmonate-dependent fu... | KEEP AS NON CORE | Summary: MYC2 negatively regulates tryptophan and indole-glucosinolate metabolism during jasmonate signaling. Reason: PMID:17616737 reports transcriptional and functional regulation of this metabolic branch by MYC2, with negative rather than positive regulation. Retain as a non-core biological process mediated by transcriptional control; this does not imply MYC2 is a biosynthetic enzyme. Supporting Evidence: PMID:17616737 We found that MYC2 negatively regulates Trp and Trp-derived secondary metabolism such as indole glucosinolate biosynthesis during JA signaling. |
| GO:0009759 indole glucosinolate biosynthetic process | IEP PMID:17616737 MYC2 differentially modulates diverse jasmonate-dependent fu... | KEEP AS NON CORE | Summary: MYC2 negatively regulates tryptophan and indole-glucosinolate metabolism during jasmonate signaling. Reason: PMID:17616737 reports transcriptional and functional regulation of this metabolic branch by MYC2, with negative rather than positive regulation. Retain as a non-core biological process mediated by transcriptional control; this does not imply MYC2 is a biosynthetic enzyme. Supporting Evidence: PMID:17616737 We found that MYC2 negatively regulates Trp and Trp-derived secondary metabolism such as indole glucosinolate biosynthesis during JA signaling. |
| GO:0009759 indole glucosinolate biosynthetic process | IMP PMID:17616737 MYC2 differentially modulates diverse jasmonate-dependent fu... | KEEP AS NON CORE | Summary: MYC2 negatively regulates tryptophan and indole-glucosinolate metabolism during jasmonate signaling. Reason: PMID:17616737 reports transcriptional and functional regulation of this metabolic branch by MYC2, with negative rather than positive regulation. Retain as a non-core biological process mediated by transcriptional control; this does not imply MYC2 is a biosynthetic enzyme. Supporting Evidence: PMID:17616737 We found that MYC2 negatively regulates Trp and Trp-derived secondary metabolism such as indole glucosinolate biosynthesis during JA signaling. |
| GO:0009269 response to desiccation | IEP PMID:9368419 Role of arabidopsis MYC and MYB homologs in drought- and abs... | KEEP AS NON CORE | Summary: MYC2/rd22BP1 binds a defined cis-regulatory recognition site in the rd22 promoter. Reason: The original study directly measured induction of rd22BP1/MYC2 transcripts by dehydration and ABA. These are supported stress/hormone responses outside the central jasmonate signaling role. Supporting Evidence: PMID:9368419 RNA gel blot analysis revealed that transcription of the rd22BP1 gene is induced by dehydration stress and ABA treatment, and its induction precedes that of rd22. |
| GO:0009611 response to wounding | IEP PMID:17675405 A downstream mediator in the growth repression limb of the j... | KEEP AS NON CORE | Summary: IEP from the Yan et al. microarray screen for jasmonate-dependent wound-inducible transcripts; the curator's evidence that MYC2 (At1g32640) is among them is not in the cached text. Reason: The cached text of PMID:17675405 never names MYC2 or At1g32640; the MYC2-specific result is presumably in a table or supplement that is not available in the cache, so the curator IEP is deferred to rather than overruled. It is consistent with the independently supported wound response of MYC2 (TAS row, PMID:9680973, where the jin1/myc2 mutant blocks JA-responsive transcription in a wound-signalling study). Kept as non-core for consistency with that row; an expression response is a downstream output, not the core transcription-factor function. Supporting Evidence: PMID:9680973 JA activation via this pathway was blocked in the A. thaliana JA-insensitive mutants jin1, jin4 and coi1 |
| GO:0009611 response to wounding | TAS PMID:9680973 Reversible protein phosphorylation regulates jasmonic acid-d... | KEEP AS NON CORE | Summary: The jin1 mutant disrupts jasmonate-responsive transcription in a wound-signaling study. Reason: This is a well-supported downstream process, but the core function is transcription factor activity in jasmonate signaling. Supporting Evidence: PMID:9680973 JA activation via this pathway was blocked in the A. thaliana JA-insensitive mutants jin1, jin4 and coi1 |
| GO:0009737 response to abscisic acid | IEP PMID:9368419 Role of arabidopsis MYC and MYB homologs in drought- and abs... | KEEP AS NON CORE | Summary: ABA treatment induces rd22BP1/MYC2 transcription before induction of the ABA-responsive rd22 gene. Reason: The original study directly measured induction of rd22BP1/MYC2 transcripts by dehydration and ABA. These are supported stress/hormone responses outside the central jasmonate signaling role. Supporting Evidence: PMID:9368419 RNA gel blot analysis revealed that transcription of the rd22BP1 gene is induced by dehydration stress and ABA treatment, and its induction precedes that of rd22. |
| GO:0009753 response to jasmonic acid | IMP PMID:21242320 The bHLH transcription factor MYC3 interacts with the Jasmon... | MODIFY | Summary: MYC2 is a JAZ-regulated transcriptional effector in jasmonate signaling. Reason: The original PMID:21242320 abstract explicitly describes MYC2 interaction with JAZ repressors and mediation of jasmonate responses, although the new experiments foreground MYC3. The independently verified JAZ-regulation and transcription assays cited here establish MYC2 as a pathway component. Replace the broad response term with jasmonic acid mediated signaling pathway; this is a specificity judgment, not a claim that the MYC3-focused abstract exposes every MYC2 experiment. Proposed replacements: jasmonic acid mediated signaling pathway Supporting Evidence: PMID:21242320 The bHLH transcription factor MYC2 was found to interact with JAZs and mediate JA responses including JA-inhibitory root growth. PMID:17637675 Moreover, JAI3 negatively regulates the key transcriptional activator of jasmonate responses, MYC2. PMID:21321051 MYC2, MYC3, and MYC4 were all capable of inducing expression of JAZ::GUS reporter constructs following transfection of carrot protoplasts. |
| GO:0009963 positive regulation of flavonoid biosynthetic process | IEP PMID:17616737 MYC2 differentially modulates diverse jasmonate-dependent fu... | KEEP AS NON CORE | Summary: MYC2 promotes jasmonate-regulated flavonoid biosynthesis. Reason: The original transcriptional analysis and functional comparisons support positive regulation of flavonoid biosynthesis. Retain the regulatory process as non-core context for the transcription factor. Supporting Evidence: PMID:17616737 We also provided evidence that MYC2 is a positive regulator of enzymes and regulators involved in JA-mediated flavonoid biosynthesis. |
| GO:0010374 stomatal complex development | IGI PMID:29496884 Jasmonate Negatively Regulates Stomatal Development in Arabi... | KEEP AS NON CORE | Summary: MYC2/3/4 redundantly mediate jasmonate-dependent inhibition of stomatal development. Reason: MYC2/3/4 redundantly mediate jasmonate-dependent inhibition of stomatal development. Retain as a supported context-specific process controlled by the transcription factor. Supporting Evidence: PMID:29496884 three MYC transcription factors involved in jasmonate signaling, MYC2, MYC3, and MYC4, were found to redundantly modulate jasmonate-inhibited stomatal development. |
| GO:0045893 positive regulation of DNA-templated transcription | IDA PMID:21321051 Characterization of JAZ-interacting bHLH transcription facto... | ACCEPT | Summary: MYC2 directly activates a JAZ promoter reporter in transfected protoplasts. Reason: Transcriptional regulation is a core biological role of MYC2 as a bHLH transcription factor. Supporting Evidence: PMID:21321051 MYC2, MYC3, and MYC4 were all capable of inducing expression of JAZ::GUS reporter constructs following transfection of carrot protoplasts. |
| GO:0045893 positive regulation of DNA-templated transcription | IDA PMID:9368419 Role of arabidopsis MYC and MYB homologs in drought- and abs... | ACCEPT | Summary: MYC2/rd22BP1 binds a defined cis-regulatory recognition site in the rd22 promoter. Reason: Transcriptional regulation is a core biological role of MYC2 as a bHLH transcription factor. Supporting Evidence: PMID:9368419 we demonstrated that both the rd22BP1 and ATMYB2 proteins activate transcription of the rd22 promoter fused to the beta-glucuronidase reporter gene. |
| GO:0106167 extracellular ATP signaling | IMP PMID:30630869 Extracellular ATP Shapes a Defense-Related Transcriptome Bot... | KEEP AS NON CORE | Summary: MYC transcription factors contribute to transcriptional responses downstream of extracellular ATP perception. Reason: MYC transcription factors contribute to transcriptional responses downstream of extracellular ATP perception. Retain as a supported context-specific process controlled by the transcription factor. Supporting Evidence: PMID:30630869 We further tested the ATP-induced genes in knockout mutants of transcription factors, demonstrating that MYCs acting downstream of the jasmonate receptor complex and calmodulin-binding transcription activators are nuclear transducers of P2K1-mediated extracellular ATP signaling. |
| GO:2000068 regulation of defense response to insect | IMP PMID:17616737 MYC2 differentially modulates diverse jasmonate-dependent fu... | KEEP AS NON CORE | Summary: MYC2 promotes jasmonate-mediated insect resistance. Reason: The study reports increased MYC2-dependent resistance to Helicoverpa armigera. This defense phenotype is a supported non-core output of transcriptional regulation. Supporting Evidence: PMID:17616737 MYC2 positively regulates JA-mediated resistance to insect pests, such as Helicoverpa armigera |
| GO:2000652 regulation of secondary cell wall biogenesis | IMP PMID:30242037 Blue Light Regulates Secondary Cell Wall Thickening via MYC2... | KEEP AS NON CORE | Summary: MYC2 and MYC4 directly bind the NST1 promoter in the secondary-wall regulatory network. Reason: MYC2 and MYC4 directly bind the NST1 promoter in the secondary-wall regulatory network. Retain as a supported context-specific process controlled by the transcription factor. Supporting Evidence: PMID:30242037 The activation is caused by direct binding of MYC2/MYC4 to the NST1 promoter. |
| GO:0000976 transcription cis-regulatory region binding | IPI PMID:25352272 Promoter-based integration in plant defense regulation. | ACCEPT | Summary: MYC2 is a nuclear bHLH transcription factor that binds cis-regulatory DNA elements in target promoters. Reason: The source is a systematic promoter-interaction study whose accessible text does not identify the MYC2-specific promoter pair. The general assertion of MYC2 cis-regulatory DNA binding is independently established by the rd22 and NST1 studies, so ACCEPT is retained while deferring to the original curator for the network experiment. Supporting Evidence: PMID:9368419 The rd22BP1 protein binds specifically to the first MYC recognition site in the 67-bp fragment. PMID:30242037 The activation is caused by direct binding of MYC2/MYC4 to the NST1 promoter. |
| GO:0000976 transcription cis-regulatory region binding | IPI PMID:25533953 An Arabidopsis gene regulatory network for secondary cell wa... | ACCEPT | Summary: MYC2 is a nuclear bHLH transcription factor that binds cis-regulatory DNA elements in target promoters. Reason: The source is a systematic promoter-interaction study whose accessible text does not identify the MYC2-specific promoter pair. The general assertion of MYC2 cis-regulatory DNA binding is independently established by the rd22 and NST1 studies, so ACCEPT is retained while deferring to the original curator for the network experiment. Supporting Evidence: PMID:9368419 The rd22BP1 protein binds specifically to the first MYC recognition site in the 67-bp fragment. PMID:30242037 The activation is caused by direct binding of MYC2/MYC4 to the NST1 promoter. |
| GO:0000976 transcription cis-regulatory region binding | IPI PMID:27923776 Establishment of Expression in the SHORTROOT-SCARECROW Trans... | ACCEPT | Summary: MYC2 is a nuclear bHLH transcription factor that binds cis-regulatory DNA elements in target promoters. Reason: The source is a systematic promoter-interaction study whose accessible text does not identify the MYC2-specific promoter pair. The general assertion of MYC2 cis-regulatory DNA binding is independently established by the rd22 and NST1 studies, so ACCEPT is retained while deferring to the original curator for the network experiment. Supporting Evidence: PMID:9368419 The rd22BP1 protein binds specifically to the first MYC recognition site in the 67-bp fragment. PMID:30242037 The activation is caused by direct binding of MYC2/MYC4 to the NST1 promoter. |
| GO:0000976 transcription cis-regulatory region binding | IPI PMID:30242037 Blue Light Regulates Secondary Cell Wall Thickening via MYC2... | ACCEPT | Summary: MYC2 and MYC4 directly bind the NST1 promoter in the secondary-wall regulatory network. Reason: transcription cis-regulatory region binding is a direct molecular function of MYC2 and is central to its transcription factor role. Supporting Evidence: PMID:30242037 The activation is caused by direct binding of MYC2/MYC4 to the NST1 promoter. |
| GO:0003677 DNA binding | IDA PMID:28514654 Crystal Structure of Tetrameric Arabidopsis MYC2 Reveals the... | MODIFY | Summary: MYC2 binds G-box DNA as a tetramer in the crystal structure. Reason: The cited study demonstrates MYC2 binding to a defined promoter motif (G-box DNA in PMID:28514654; the rd22 MYC recognition site in PMID:9368419). The proposed specific DNA-binding term captures the assayed function better than undifferentiated DNA binding. Proposed replacements: transcription cis-regulatory region binding sequence-specific DNA binding Supporting Evidence: PMID:28514654 Here, we report the 2.7 Å crystal structure of the MYC2 bHLH domain complexed with G-box DNA, showing a cis-tetrameric structure. |
| GO:0003677 DNA binding | IDA PMID:9368419 Role of arabidopsis MYC and MYB homologs in drought- and abs... | MODIFY | Summary: MYC2/rd22BP1 binds a defined cis-regulatory recognition site in the rd22 promoter. Reason: The cited study demonstrates MYC2 binding to a defined promoter motif (G-box DNA in PMID:28514654; the rd22 MYC recognition site in PMID:9368419). The proposed specific DNA-binding term captures the assayed function better than undifferentiated DNA binding. Proposed replacements: transcription cis-regulatory region binding sequence-specific DNA binding Supporting Evidence: PMID:9368419 The rd22BP1 protein binds specifically to the first MYC recognition site in the 67-bp fragment. |
| GO:0003700 DNA-binding transcription factor activity | IEA GO_REF:0000002 | ACCEPT | Summary: MYC2 is a nuclear bHLH transcription factor that binds cis-regulatory DNA elements in target promoters. Reason: The domain/family-based inference is independently corroborated by MYC2 DNA-binding and promoter-transactivation experiments in PMID:9368419 and PMID:21321051. Retain the transcription-factor assertion. |
| GO:0003700 DNA-binding transcription factor activity | ISS PMID:11118137 Arabidopsis transcription factors: genome-wide comparative a... | ACCEPT | Summary: MYC2 is a nuclear bHLH transcription factor that binds cis-regulatory DNA elements in target promoters. Reason: The domain/family-based inference is independently corroborated by MYC2 DNA-binding and promoter-transactivation experiments in PMID:9368419 and PMID:21321051. Retain the transcription-factor assertion. |
| GO:0003700 DNA-binding transcription factor activity | ISS PMID:12679534 The basic helix-loop-helix transcription factor family in pl... | ACCEPT | Summary: MYC2 is a nuclear bHLH transcription factor that binds cis-regulatory DNA elements in target promoters. Reason: The domain/family-based inference is independently corroborated by MYC2 DNA-binding and promoter-transactivation experiments in PMID:9368419 and PMID:21321051. Retain the transcription-factor assertion. |
| GO:0005515 protein binding | IPI PMID:17637675 The JAZ family of repressors is the missing link in jasmonat... | REMOVE | Summary: JAZ/JAI3 repression regulates MYC2 within the core jasmonate pathway. Reason: JAZ/JAI3 repression regulates MYC2 within the core jasmonate pathway. Remove the uninformative generic protein-binding annotation while retaining this interaction evidence. This does not reject the interaction, and interaction alone does not establish a distinct molecular activity beyond the supported functions reviewed elsewhere. Supporting Evidence: PMID:17637675 Moreover, JAI3 negatively regulates the key transcriptional activator of jasmonate responses, MYC2. |
| GO:0005515 protein binding | IPI PMID:18547396 A critical role of two positively charged amino acids in the... | REMOVE | Summary: JAZ1 and JAZ9 interact with MYC2 even when residues required for COI1 recognition are mutated. Reason: JAZ1 and JAZ9 interact with MYC2 even when residues required for COI1 recognition are mutated. Remove the uninformative generic protein-binding annotation while retaining this interaction evidence. This does not reject the interaction, and interaction alone does not establish a distinct molecular activity beyond the supported functions reviewed elsewhere. Supporting Evidence: PMID:18547396 Mutations of these two residues did not affect the ability of JAZ1 and JAZ9 to interact with the transcription factor AtMYC2. |
| GO:0005515 protein binding | IPI PMID:19309455 The ZIM domain mediates homo- and heteromeric interactions b... | REMOVE | Summary: Pull-down and yeast two-hybrid assays establish JAZ-MYC2 binding. Reason: Pull-down and yeast two-hybrid assays establish JAZ-MYC2 binding. Remove the uninformative generic protein-binding annotation while retaining this interaction evidence. This does not reject the interaction, and interaction alone does not establish a distinct molecular activity beyond the supported functions reviewed elsewhere. Supporting Evidence: PMID:19309455 we show that interaction with MYC2 is a common feature of the JAZ family, as most JAZ proteins can bind MYC2 in pull-down and yeast two-hybrid assays. |
| GO:0005515 protein binding | IPI PMID:21737749 Derepression of ethylene-stabilized transcription factors (E... | REMOVE | Summary: Interaction assay from a study of JAZ regulation of EIN3/EIL1 and MYC2; the MYC2-specific pair (likely a JAZ protein) is not exposed in the extracted text. Reason: Handled consistently with the other MYC2 protein-binding rows. Remove the uninformative generic protein-binding annotation while retaining this interaction evidence. This does not reject the interaction, and interaction alone does not establish a distinct molecular activity beyond the supported functions reviewed elsewhere. The interaction is not disputed; the informative MYC2 functions (DNA-binding transcription factor activity, JAZ-repressed transcription) are captured by other annotations. |
| GO:0005515 protein binding | IPI PMID:23169619 Transcription factor-dependent nuclear localization of a tra... | REMOVE | Summary: MYC2 interaction contributes to nuclear targeting of JAZ repressors. Reason: MYC2 interaction contributes to nuclear targeting of JAZ repressors. Remove the uninformative generic protein-binding annotation while retaining this interaction evidence. This does not reject the interaction, and interaction alone does not establish a distinct molecular activity beyond the supported functions reviewed elsewhere. Supporting Evidence: PMID:23169619 physical interaction with MYC2 appears to play an active role in the nuclear targeting of JAZ1 and JAZ9, and the nuclear localization of JAZ9 was compromised in myc2 mutant plants. |
| GO:0005515 protein binding | IPI PMID:24399301 Interaction between MYC2 and ETHYLENE INSENSITIVE3 modulates... | REMOVE | Summary: MYC2 binds EIN3 and represses its transcriptional activity, mediating JA/ET antagonism. Reason: MYC2 binds EIN3 and represses its transcriptional activity, mediating JA/ET antagonism. Remove the uninformative generic protein-binding annotation while retaining this interaction evidence. This does not reject the interaction, and interaction alone does not establish a distinct molecular activity beyond the supported functions reviewed elsewhere. Supporting Evidence: PMID:24399301 MYC2 interacts with EIN3 to attenuate the transcriptional activity of EIN3 and repress ET-enhanced apical hook curvature. |
| GO:0005515 protein binding | IPI PMID:26258305 Structural basis of JAZ repression of MYC transcription fact... | REMOVE | Summary: The study directly maps a JAZ9-interacting region in MYC2 as well as MYC3/MYC4; MYC3-focused structural results do not imply MYC2 was untested. Reason: The study directly maps a JAZ9-interacting region in MYC2 as well as MYC3/MYC4; MYC3-focused structural results do not imply MYC2 was untested. Remove the uninformative generic protein-binding annotation while retaining this interaction evidence. This does not reject the interaction, and interaction alone does not establish a distinct molecular activity beyond the supported functions reviewed elsewhere. Supporting Evidence: PMID:26258305 A conserved ~200 amino acid (aa 55-259, aa 44-234 and aa 55-253 in MYC2, MYC3 and MYC4, respectively) region within the N-termini of all three proteins that encompasses the previously defined JAZ-interacting domain (JID)13,14 and the transcription activation domain (TAD)13,15 was sufficient to interact with JAZ9 |
| GO:0005515 protein binding | IPI PMID:27357749 An ABA-increased interaction of the PYL6 ABA receptor with M... | REMOVE | Summary: PYL6-MYC2 interaction is supported by two in-planta assays. Reason: PYL6-MYC2 interaction is supported by two in-planta assays. Remove the uninformative generic protein-binding annotation while retaining this interaction evidence. This does not reject the interaction, and interaction alone does not establish a distinct molecular activity beyond the supported functions reviewed elsewhere. Supporting Evidence: PMID:27357749 PYL6 and MYC2 interact in planta based on bimolecular fluorescence complementation and co-immunoprecipitation of the proteins. |
| GO:0005515 protein binding | IPI PMID:28650476 CrY2H-seq: a massively multiplexed assay for deep-coverage i... | REMOVE | Summary: High-throughput CrY2H-seq interactome screen including MYC2 interaction pairs. Reason: Handled consistently with the other MYC2 protein-binding rows. Remove the uninformative generic protein-binding annotation while retaining this interaction evidence. This does not reject the interaction, and interaction alone does not establish a distinct molecular activity beyond the supported functions reviewed elsewhere. A screen-level binary interaction adds no specific molecular-function information. |
| GO:0005515 protein binding | IPI PMID:32490347 A point-to-point protein-protein interaction assay reveals t... | REMOVE | Summary: Hormone-signaling yeast two-hybrid interaction map in which MYC2 appears among jasmonate-pathway baits/preys. Reason: Handled consistently with the other MYC2 protein-binding rows. Remove the uninformative generic protein-binding annotation while retaining this interaction evidence. This does not reject the interaction, and interaction alone does not establish a distinct molecular activity beyond the supported functions reviewed elsewhere. |
| GO:0005515 protein binding | IPI PMID:32612234 Extensive signal integration by the phytohormone protein net... | REMOVE | Summary: Large-scale hormone-signaling protein interaction network including MYC2. Reason: Handled consistently with the other MYC2 protein-binding rows. Remove the uninformative generic protein-binding annotation while retaining this interaction evidence. This does not reject the interaction, and interaction alone does not establish a distinct molecular activity beyond the supported functions reviewed elsewhere. |
| GO:0005515 protein binding | IPI PMID:39489485 Jasmonate induces translation of the Arabidopsis transfer RN... | REMOVE | Summary: YL1 physically interacts with and activates MYC2. Reason: YL1 physically interacts with and activates MYC2. Remove the uninformative generic protein-binding annotation while retaining this interaction evidence. This does not reject the interaction, and interaction alone does not establish a distinct molecular activity beyond the supported functions reviewed elsewhere. Supporting Evidence: PMID:39489485 Once translated, YL1 acts as an activator of the MYC2 transcription factor through direct interaction |
| GO:0019900 kinase binding | IPI PMID:24890857 Inverse modulation of the energy sensor Snf1-related protein... | KEEP AS NON CORE | Summary: MYC2 interacts with the SnRK1 kinase AKIN10, which phosphorylation-dependently promotes proteasomal turnover of MYC2. Reason: The abstract reports a novel molecular interaction between AKIN10 and AtMYC2 and AKIN10-kinase-dependent MYC2 modification; the curator's IPI (full text) is retained. MYC2 is the kinase substrate, so kinase binding records a regulatory input on MYC2 stability rather than a MYC2 activity; non-core. Supporting Evidence: PMID:24890857 Biochemical analyses showed that AKIN10 negatively modulates AtMYC2 protein accumulation via proteasome activity upon AKIN10 kinase activity-dependent protein modification. |
| GO:0043565 sequence-specific DNA binding | IDA PMID:25490915 Virulence factors of geminivirus interact with MYC2 to subve... | ACCEPT | Summary: MYC2 is a nuclear bHLH transcription factor that binds cis-regulatory DNA elements in target promoters. Reason: The recovered full text directly demonstrates MYC2 binding to the TPS10 G-box-like 2 element by electrophoretic mobility shift assay, with mutant-element and competition controls, and in-vivo ChIP supports promoter occupancy. Supporting Evidence: PMID:25490915 His-MYC2 can bind to G-box-like 2 (CACATG) but not to G-box-like 1 (CAAGTG) nor to a mutant of G-box-like 2 (TTCAAA) |
| GO:0043565 sequence-specific DNA binding | IPI PMID:25490915 Virulence factors of geminivirus interact with MYC2 to subve... | ACCEPT | Summary: MYC2 is a nuclear bHLH transcription factor that binds cis-regulatory DNA elements in target promoters. Reason: The recovered full text directly demonstrates MYC2 binding to the TPS10 G-box-like 2 element by electrophoretic mobility shift assay, with mutant-element and competition controls, and in-vivo ChIP supports promoter occupancy. Supporting Evidence: PMID:25490915 His-MYC2 can bind to G-box-like 2 (CACATG) but not to G-box-like 1 (CAAGTG) nor to a mutant of G-box-like 2 (TTCAAA) |
| GO:0046983 protein dimerization activity | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: protein dimerization activity is compatible with bHLH transcription factor oligomerization behavior. Reason: The bHLH-domain inference supports protein association involved in DNA binding. PMID:28514654 demonstrates that full-length MYC2 forms stable tetramers; retain generic dimerization as a non-core broad association descriptor while capturing the directly established tetrameric mechanism separately. |
| GO:0006355 regulation of DNA-templated transcription | IEA GO_REF:0000002 | ACCEPT | Summary: MYC2 regulates transcriptional output of jasmonate-responsive and stress/metabolism genes. Reason: The domain/family-based inference is independently corroborated by MYC2 DNA-binding and promoter-transactivation experiments in PMID:9368419 and PMID:21321051. Retain the transcription-factor assertion. |
| GO:0010468 regulation of gene expression | IEA GO_REF:0000117 | MODIFY | Summary: MYC2 regulates gene expression, but the ARBA term is broader than the DNA-templated transcriptional regulation supported for this nuclear bHLH transcription factor. Reason: The domain/family-based inference is independently corroborated by MYC2 DNA-binding and promoter-transactivation experiments in PMID:9368419 and PMID:21321051. Refine generic regulation of gene expression to transcriptional regulation. Proposed replacements: regulation of DNA-templated transcription |
| GO:0048364 root development | IMP PMID:17616737 MYC2 differentially modulates diverse jasmonate-dependent fu... | KEEP AS NON CORE | Summary: MYC2 participates in hormonal control of root elongation. Reason: The original study demonstrates MYC2-dependent sensitivity of root elongation to auxin transport inhibitors, supporting a non-core developmental process. Supporting Evidence: PMID:17616737 MYC2 is required for the inhibition of root elongation by auxin transport inhibitors. |
| GO:0051289 protein homotetramerization | IDA PMID:28514654 Crystal Structure of Tetrameric Arabidopsis MYC2 Reveals the... | ACCEPT | Summary: MYC2 homotetramerization enhances DNA binding and transcriptional regulation. Reason: Tetramerization is a directly demonstrated molecular mechanism of MYC2 transcription factor activity, rather than an incidental association. The study measures solution and DNA-bound tetramers and tests their functional importance. Supporting Evidence: PMID:28514654 Biochemical assays confirmed that full-length MYC2 forms a stable homo-tetramer both in solution and in DNA-bound states, whereas MYC3 forms only a homodimer. |
| GO:0005634 nucleus | IEA GO_REF:0000044 | ACCEPT | Summary: MYC2 acts in the nucleus. Reason: Nuclear localization is directly demonstrated by Venus-MYC2 imaging in PMID:27357749, consistent with promoter binding and transcriptional regulation. For source-specific localization rows whose accessible abstract omits imaging, retain the biologically established location and defer to the original curator. |
| GO:0005634 nucleus | IEA GO_REF:0000117 | ACCEPT | Summary: MYC2 acts in the nucleus. Reason: Nuclear localization is directly demonstrated by Venus-MYC2 imaging in PMID:27357749, consistent with promoter binding and transcriptional regulation. For source-specific localization rows whose accessible abstract omits imaging, retain the biologically established location and defer to the original curator. |
| GO:0005634 nucleus | ISM GO_REF:0000122 | ACCEPT | Summary: MYC2 acts in the nucleus. Reason: Nuclear localization is directly demonstrated by Venus-MYC2 imaging in PMID:27357749, consistent with promoter binding and transcriptional regulation. For source-specific localization rows whose accessible abstract omits imaging, retain the biologically established location and defer to the original curator. |
| GO:0005634 nucleus | IDA PMID:21242320 The bHLH transcription factor MYC3 interacts with the Jasmon... | ACCEPT | Summary: MYC2 acts in the nucleus. Reason: Nuclear localization is directly demonstrated by Venus-MYC2 imaging in PMID:27357749, consistent with promoter binding and transcriptional regulation. For source-specific localization rows whose accessible abstract omits imaging, retain the biologically established location and defer to the original curator. Supporting Evidence: PMID:27357749 Venus-MYC2 was targeted only to the nucleus |
| GO:0005634 nucleus | IDA PMID:24890857 Inverse modulation of the energy sensor Snf1-related protein... | ACCEPT | Summary: MYC2 acts in the nucleus. Reason: Nuclear localization is directly demonstrated by Venus-MYC2 imaging in PMID:27357749, consistent with promoter binding and transcriptional regulation. For source-specific localization rows whose accessible abstract omits imaging, retain the biologically established location and defer to the original curator. Supporting Evidence: PMID:27357749 Venus-MYC2 was targeted only to the nucleus |
| GO:0005634 nucleus | IDA PMID:25490915 Virulence factors of geminivirus interact with MYC2 to subve... | ACCEPT | Summary: MYC2 acts in the nucleus. Reason: The recovered full text directly reports nuclear localization of Arabidopsis MYC2 and its Nicotiana counterpart in Supplemental Figure 8. Supporting Evidence: PMID:25490915 At-MYC2 and Nb-MYC2 specifically localized in the nucleus. |
| GO:0005634 nucleus | EXP PMID:27357749 An ABA-increased interaction of the PYL6 ABA receptor with M... | ACCEPT | Summary: MYC2 acts in the nucleus. Reason: Nuclear localization is directly demonstrated by Venus-MYC2 imaging in PMID:27357749, consistent with promoter binding and transcriptional regulation. For source-specific localization rows whose accessible abstract omits imaging, retain the biologically established location and defer to the original curator. Supporting Evidence: PMID:27357749 Venus-MYC2 was targeted only to the nucleus |
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Download this section (compressed HTML)Q: Which MYC2 protein interactions should be represented as specific transcriptional co-regulator or repressor-complex biology rather than generic protein binding?
Q: Which downstream jasmonate phenotypes warrant direct MYC2 process annotations versus remaining non-core transcriptional outputs?
Experiment: Combine native MYC2 ChIP-seq with rapid JA treatment and myc2 loss-of-function RNA-seq to separate direct targets from indirect downstream outputs.
Hypothesis: MYC2 direct binding sites define the subset of jasmonate-response annotations that should be curated as direct regulatory targets.
Type: ChIP-seq perturbation
Experiment: Quantitatively map MYC2 complexes before and after jasmonate and test partner-specific mutants for target-gene and phenotype effects.
Hypothesis: JAZ, MED25, kinase, and photoreceptor interactions modulate distinct MYC2 target classes.
Type: interaction mapping
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