TPL

Summary: Negative regulation of transcription by RNA polymerase II, inferred for the TPL/TPR clade.

Reason: TPL represses target genes as a Groucho/Tup1-type co-repressor, acting through Mediator and histone deacetylation.

Summary: Transcription corepressor activity inferred for the TPL/TPR family.

Reason: Core function: TPL is recruited by EAR-motif repressors (Aux/IAAs, NINJA, many TFs) and represses their targets.

Summary: TPL interaction with the DNA-binding transcription factor WUS (Protein WUSCHEL (AtWUS) (Plant growth activator 6)), reported by this study. TPL is recruited by EAR-motif or other repression-domain-containing transcription factors to repress their target genes.

Reason: Generic protein binding is uninformative. Direct binding of a DNA-binding transcription factor by the co-repressor is captured by DNA-binding transcription factor binding.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor WUS (Protein WUSCHEL (AtWUS) (Plant growth activator 6)), reported by this study. TPL is recruited by EAR-motif or other repression-domain-containing transcription factors to repress their target genes.

Reason: Generic protein binding is uninformative. Direct binding of a DNA-binding transcription factor by the co-repressor is captured by DNA-binding transcription factor binding.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with AFP2 (Ninja-family protein AFP2), reported by this study. This partner is a non-DNA-binding repressor or adaptor (e.g. NINJA, AFP, JAZ, KIX, microProtein or TWA1) that bridges TPL to transcription factors.

Reason: The full paper explicitly reports Y2H interaction of AFP proteins with TPL (Supplementary Fig. 4e), separately from its NINJA-JAZ result. AFP2/AFP3 are non-DNA-binding transcriptional adaptors, so refine bare binding to transcription coregulator binding. The quoted group-level result supports AFP-TPL interaction; the exact partner accession is retained from the curator-read experimental annotation and is not claimed to occur in the quoted sentence.

Proposed replacements: transcription coregulator binding

Summary: TPL interaction with AFP3 (Ninja-family protein AFP3), reported by this study. This partner is a non-DNA-binding repressor or adaptor (e.g. NINJA, AFP, JAZ, KIX, microProtein or TWA1) that bridges TPL to transcription factors.

Reason: The full paper explicitly reports Y2H interaction of AFP proteins with TPL (Supplementary Fig. 4e), separately from its NINJA-JAZ result. AFP2/AFP3 are non-DNA-binding transcriptional adaptors, so refine bare binding to transcription coregulator binding. The quoted group-level result supports AFP-TPL interaction; the exact partner accession is retained from the curator-read experimental annotation and is not claimed to occur in the quoted sentence.

Proposed replacements: transcription coregulator binding

Summary: TPL interaction with AFPH2 (AFP homolog 2 (Novel interactor of JAZ)), reported by this study. This partner is a non-DNA-binding repressor or adaptor (e.g. NINJA, AFP, JAZ, KIX, microProtein or TWA1) that bridges TPL to transcription factors.

Reason: Generic protein binding is uninformative. Binding of a non-DNA-binding transcriptional co-regulator/adaptor is captured by transcription coregulator binding.

Proposed replacements: transcription coregulator binding

Summary: TPL interaction with AFPH2 (AFP homolog 2 (Novel interactor of JAZ)), reported by this study. This partner is a non-DNA-binding repressor or adaptor (e.g. NINJA, AFP, JAZ, KIX, microProtein or TWA1) that bridges TPL to transcription factors.

Reason: Generic protein binding is uninformative. Binding of a non-DNA-binding transcriptional co-regulator/adaptor is captured by transcription coregulator binding.

Proposed replacements: transcription coregulator binding

Summary: TPL interaction with the DNA-binding transcription factor ERF10 (Ethylene-responsive transcription factor 10), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor ERF10 (Ethylene-responsive transcription factor 10) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the Aux/IAA protein IAA10, reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the Aux/IAA protein IAA10 interaction and its independently recognizable regulatory partner class support transcription corepressor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: transcription corepressor binding

Summary: TPL interaction with the Aux/IAA protein IAA3, reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the Aux/IAA protein IAA3 interaction and its independently recognizable regulatory partner class support transcription corepressor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: transcription corepressor binding

Summary: TPL interaction with the DNA-binding transcription factor WIP3 (Zinc finger protein WIP3 (WIP-domain protein 3) (AtWIP3)), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor WIP3 (Zinc finger protein WIP3 (WIP-domain protein 3) (AtWIP3)) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with At1g12120 (uncharacterized protein), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012). The screen alone does not determine its transcriptional consequence.

Reason: Remove the uninformative bare protein-binding assertion under project policy; the measured screen interaction remains documented. The available source does not establish a more informative functional interaction for this pair, and removal is not a claim that binding is false.

Summary: TPL interaction with the DNA-binding transcription factor RAV1 (AP2/ERF and B3 domain-containing transcription factor RAV1), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor RAV1 (AP2/ERF and B3 domain-containing transcription factor RAV1) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with TIFY11A (Protein TIFY 11A (Jasmonate ZIM domain-containing protein 5)), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported TIFY11A (Protein TIFY 11A (Jasmonate ZIM domain-containing protein 5)) interaction and its independently recognizable regulatory partner class support transcription coregulator binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: transcription coregulator binding

Summary: TPL interaction with the DNA-binding transcription factor RVE7 (Protein REVEILLE 7), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor RVE7 (Protein REVEILLE 7) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor TEM1 (AP2/ERF and B3 domain-containing transcription repressor TEM1), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor TEM1 (AP2/ERF and B3 domain-containing transcription repressor TEM1) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor ZAT10 (Zinc finger protein ZAT10 (Salt-tolerance zinc finger)), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor ZAT10 (Zinc finger protein ZAT10 (Salt-tolerance zinc finger)) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor ERF12 (Ethylene-responsive transcription factor 12), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor ERF12 (Ethylene-responsive transcription factor 12) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor ERF11 (Ethylene-responsive transcription factor 11), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor ERF11 (Ethylene-responsive transcription factor 11) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor WOX4 (WUSCHEL-related homeobox 4), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor WOX4 (WUSCHEL-related homeobox 4) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor ERF3 (Ethylene-responsive transcription factor 3), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor ERF3 (Ethylene-responsive transcription factor 3) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor AT1G50680 (transcription factor), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor AT1G50680 (transcription factor) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor AT1G51120 (transcription factor), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor AT1G51120 (transcription factor) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor ERF8 (Ethylene-responsive transcription factor 8), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor ERF8 (Ethylene-responsive transcription factor 8) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor TCP3 (Transcription factor TCP3), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor TCP3 (Transcription factor TCP3) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor ZFP4 (Zinc finger protein 4), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor ZFP4 (Zinc finger protein 4) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor HHO2 (Transcription factor HHO2), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor HHO2 (Transcription factor HHO2) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with TIFY11B (Protein TIFY 11B (Jasmonate ZIM domain-containing protein 6)), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported TIFY11B (Protein TIFY 11B (Jasmonate ZIM domain-containing protein 6)) interaction and its independently recognizable regulatory partner class support transcription coregulator binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: transcription coregulator binding

Summary: TPL interaction with SUVH3 (Histone-lysine N-methyltransferase, H3 lysine-9 specific SUVH3), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012). The screen alone does not determine its transcriptional consequence.

Reason: The full interactome paper identifies SUVH3/SDG19 among chromatin regulators associated with TPL/TPRs. This supports transcription coregulator binding as the informative partner class; it does not assign the partner's chromatin chemistry to TPL or prove a native regulatory outcome for this screen pair. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: transcription coregulator binding

Summary: TPL interaction with the DNA-binding transcription factor ARF17 (Auxin response factor 17), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor ARF17 (Auxin response factor 17) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with At1g80610 (uncharacterized protein), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012). The screen alone does not determine its transcriptional consequence.

Reason: Remove the uninformative bare protein-binding assertion under project policy; the measured screen interaction remains documented. The available source does not establish a more informative functional interaction for this pair, and removal is not a claim that binding is false.

Summary: TPL interaction with the DNA-binding transcription factor MYB7 (Transcription factor MYB7), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor MYB7 (Transcription factor MYB7) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor BZIP23 (Basic leucine zipper 23 (AtbZIP23) (bZIP protein 23)), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor BZIP23 (Basic leucine zipper 23 (AtbZIP23) (bZIP protein 23)) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with MSI4 (WD-40 repeat-containing protein MSI4), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012). The screen alone does not determine its transcriptional consequence.

Reason: The full interactome paper identifies MSI4/FVE among chromatin regulators associated with TPL/TPRs. This supports transcription coregulator binding as the informative partner class; it does not assign the partner's chromatin chemistry to TPL or prove a native regulatory outcome for this screen pair. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: transcription coregulator binding

Summary: TPL interaction with the DNA-binding transcription factor RAP2-7 (Ethylene-responsive transcription factor RAP2-7), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor RAP2-7 (Ethylene-responsive transcription factor RAP2-7) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor ARF70 (B3 domain-containing protein At2g31720), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor ARF70 (B3 domain-containing protein At2g31720) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the Aux/IAA protein IAA13, reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the Aux/IAA protein IAA13 interaction and its independently recognizable regulatory partner class support transcription corepressor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: transcription corepressor binding

Summary: TPL interaction with the DNA-binding transcription factor ASR3 (Trihelix transcription factor ASR3 (Protein ARABIDOPSIS SH4-RELATED3)), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor ASR3 (Trihelix transcription factor ASR3 (Protein ARABIDOPSIS SH4-RELATED3)) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor ARF31 (B3 domain-containing protein At2g36080), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor ARF31 (B3 domain-containing protein At2g36080) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor AS1 (Transcription factor AS1), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor AS1 (Transcription factor AS1) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor ZFP10 (Zinc finger protein 10 (AtZFP10)), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor ZFP10 (Zinc finger protein 10 (AtZFP10)) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor At2g38300 (Myb-like HTH transcriptional regulator), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor At2g38300 (Myb-like HTH transcriptional regulator) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor ZFP8 (Zinc finger protein 8), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor ZFP8 (Zinc finger protein 8) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with RMA (At2g44410) (RING membrane-anchor E3 ubiquitin ligase), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012). The screen alone does not determine its transcriptional consequence.

Reason: Remove the uninformative bare protein-binding assertion under project policy; the measured screen interaction remains documented. The available source does not establish a more informative functional interaction for this pair, and removal is not a claim that binding is false.

Summary: TPL interaction with the DNA-binding transcription factor SOC1 (MADS-box protein SOC1), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor SOC1 (MADS-box protein SOC1) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor SPL1 (SQUAMOSA promoter-binding-like protein 1), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor SPL1 (SQUAMOSA promoter-binding-like protein 1) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor LBD41 (LOB domain-containing protein 41 (ASYMMETRIC LEAVES 2-like protein 38)), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor LBD41 (LOB domain-containing protein 41 (ASYMMETRIC LEAVES 2-like protein 38)) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the Aux/IAA protein IAA16, reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the Aux/IAA protein IAA16 interaction and its independently recognizable regulatory partner class support transcription corepressor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: transcription corepressor binding

Summary: TPL interaction with the DNA-binding transcription factor NAC050 (NAC domain containing protein 50 (ANAC050)), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor NAC050 (NAC domain containing protein 50 (ANAC050)) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor NAC052 (NAC domain containing protein 52), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor NAC052 (NAC domain containing protein 52) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor ARF32 (B3 domain-containing protein At3g11580), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor ARF32 (B3 domain-containing protein At3g11580) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor TCP4 (Transcription factor TCP4 (Protein MATERNAL EFFECT EMBRYO ARREST 35)), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor TCP4 (Transcription factor TCP4 (Protein MATERNAL EFFECT EMBRYO ARREST 35)) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor ERF4 (Ethylene-responsive transcription factor 4), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor ERF4 (Ethylene-responsive transcription factor 4) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the Aux/IAA protein IAA19, reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the Aux/IAA protein IAA19 interaction and its independently recognizable regulatory partner class support transcription corepressor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: transcription corepressor binding

Summary: TPL interaction with the Aux/IAA protein IAA26, reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the Aux/IAA protein IAA26 interaction and its independently recognizable regulatory partner class support transcription corepressor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: transcription corepressor binding

Summary: TPL interaction with the DNA-binding transcription factor At3g17900 (heat-inducible transcription repressor), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor At3g17900 (heat-inducible transcription repressor) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor ERF7 (Ethylene-responsive transcription factor 7), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor ERF7 (Ethylene-responsive transcription factor 7) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the Aux/IAA protein IAA2, reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the Aux/IAA protein IAA2 interaction and its independently recognizable regulatory partner class support transcription corepressor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: transcription corepressor binding

Summary: TPL interaction with VIL1 (VIN3-like protein 1 (Protein VERNALIZATION 5)), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012). The screen alone does not determine its transcriptional consequence.

Reason: The full interactome paper identifies VRN5/VIL1 among chromatin regulators associated with TPL/TPRs. This supports transcription coregulator binding as the informative partner class; it does not assign the partner's chromatin chemistry to TPL or prove a native regulatory outcome for this screen pair. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: transcription coregulator binding

Summary: TPL interaction with AFP3 (Ninja-family protein AFP3), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported AFP3 (Ninja-family protein AFP3) interaction and its independently recognizable regulatory partner class support transcription coregulator binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: transcription coregulator binding

Summary: TPL interaction with the DNA-binding transcription factor MYB77 (Transcription factor MYB77), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor MYB77 (Transcription factor MYB77) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with At3g52250 (uncharacterized protein), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012). The screen alone does not determine its transcriptional consequence.

Reason: Remove the uninformative bare protein-binding assertion under project policy; the measured screen interaction remains documented. The available source does not establish a more informative functional interaction for this pair, and removal is not a claim that binding is false.

Summary: TPL interaction with the DNA-binding transcription factor NGA2 (B3 domain-containing transcription factor NGA2 (Protein NGATHA 2)), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor NGA2 (B3 domain-containing transcription factor NGA2 (Protein NGATHA 2)) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor MYB3R2 (Transcription factor MYB3R-2), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor MYB3R2 (Transcription factor MYB3R-2) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor HSFB2B (Heat stress transcription factor B-2b), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor HSFB2B (Heat stress transcription factor B-2b) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the Aux/IAA protein IAA1, reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the Aux/IAA protein IAA1 interaction and its independently recognizable regulatory partner class support transcription corepressor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: transcription corepressor binding

Summary: TPL interaction with the DNA-binding transcription factor HAT1 (Homeobox-leucine zipper protein HAT1), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor HAT1 (Homeobox-leucine zipper protein HAT1) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor TCP2 (Transcription factor TCP2), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor TCP2 (Transcription factor TCP2) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor ARF9 (Auxin response factor 9), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor ARF9 (Auxin response factor 9) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor NAC074 (NAC domain transcription factor), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor NAC074 (NAC domain transcription factor) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the Aux/IAA protein IAA11, reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the Aux/IAA protein IAA11 interaction and its independently recognizable regulatory partner class support transcription corepressor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: transcription corepressor binding

Summary: TPL interaction with KIX9 (KIX domain adaptor protein), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported KIX9 (KIX domain adaptor protein) interaction and its independently recognizable regulatory partner class support transcription coregulator binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: transcription coregulator binding

Summary: TPL interaction with ARI1 (Probable E3 ubiquitin-protein ligase ARI1), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012). The screen alone does not determine its transcriptional consequence.

Reason: Remove the uninformative bare protein-binding assertion under project policy; the measured screen interaction remains documented. The available source does not establish a more informative functional interaction for this pair, and removal is not a claim that binding is false.

Summary: TPL interaction with the DNA-binding transcription factor MYB32 (Transcription factor MYB32 (Myb-related protein 32) (AtMYB32)), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor MYB32 (Transcription factor MYB32 (Myb-related protein 32) (AtMYB32)) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor At4g35610 (C2H2 zinc finger protein), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor At4g35610 (C2H2 zinc finger protein) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor AP2 (Floral homeotic protein APETALA 2), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor AP2 (Floral homeotic protein APETALA 2) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor HSFB1 (Heat stress transcription factor B-1), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor HSFB1 (Heat stress transcription factor B-1) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor MYB73 (Transcription factor MYB73 (Myb-related protein 73) (AtMYB73)), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor MYB73 (Transcription factor MYB73 (Myb-related protein 73) (AtMYB73)) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor HAT22 (Homeobox-leucine zipper protein HAT22), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor HAT22 (Homeobox-leucine zipper protein HAT22) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor ZAT6 (Zinc finger protein ZAT6 (COLD INDUCED ZINC FINGER PROTEIN 2)), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor ZAT6 (Zinc finger protein ZAT6 (COLD INDUCED ZINC FINGER PROTEIN 2)) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor AT5G06250 (transcription factor), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor AT5G06250 (transcription factor) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with EMF1 (Protein EMBRYONIC FLOWER 1), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012). The screen alone does not determine its transcriptional consequence.

Reason: The full interactome paper identifies EMF1 among chromatin regulators associated with TPL/TPRs. This supports transcription coregulator binding as the informative partner class; it does not assign the partner's chromatin chemistry to TPL or prove a native regulatory outcome for this screen pair. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: transcription coregulator binding

Summary: TPL interaction with the DNA-binding transcription factor AGL15 (Agamous-like MADS-box protein AGL15), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor AGL15 (Agamous-like MADS-box protein AGL15) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor KAN1 (Transcription repressor KAN1 (Protein KANADI 1)), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor KAN1 (Transcription repressor KAN1 (Protein KANADI 1)) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor RVE1 (Protein REVEILLE 1), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor RVE1 (Protein REVEILLE 1) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor BZIP9 (Basic leucine zipper 9), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor BZIP9 (Basic leucine zipper 9) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the Aux/IAA protein IAA28, reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the Aux/IAA protein IAA28 interaction and its independently recognizable regulatory partner class support transcription corepressor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: transcription corepressor binding

Summary: TPL interaction with the Aux/IAA protein IAA4, reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the Aux/IAA protein IAA4 interaction and its independently recognizable regulatory partner class support transcription corepressor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: transcription corepressor binding

Summary: TPL interaction with the DNA-binding transcription factor GATA16 (GATA transcription factor 16), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor GATA16 (GATA transcription factor 16) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with At5g55040 (uncharacterized bromodomain protein), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012). The screen alone does not determine its transcriptional consequence.

Reason: Remove the uninformative bare protein-binding assertion under project policy; the measured screen interaction remains documented. The available source does not establish a more informative functional interaction for this pair, and removal is not a claim that binding is false.

Summary: TPL interaction with At5g57340 (uncharacterized protein), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012). The screen alone does not determine its transcriptional consequence.

Reason: Remove the uninformative bare protein-binding assertion under project policy; the measured screen interaction remains documented. The available source does not establish a more informative functional interaction for this pair, and removal is not a claim that binding is false.

Summary: TPL interaction with the DNA-binding transcription factor WOX2 (WUSCHEL-related homeobox 2), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor WOX2 (WUSCHEL-related homeobox 2) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor TOE2 (AP2-like ethylene-responsive transcription factor TOE2), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor TOE2 (AP2-like ethylene-responsive transcription factor TOE2) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor ARF2 (Auxin response factor 2), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor ARF2 (Auxin response factor 2) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with the DNA-binding transcription factor LBD37 (LOB domain-containing protein 37 (ASYMMETRIC LEAVES 2-like protein 39)), reported by the high-throughput yeast two-hybrid TPL/TPR interactome (Causier et al. 2012).

Reason: The reported the DNA-binding transcription factor LBD37 (LOB domain-containing protein 37 (ASYMMETRIC LEAVES 2-like protein 39)) interaction and its independently recognizable regulatory partner class support DNA-binding transcription factor binding. PMID:22065421 uses yeast two-hybrid screening and transcription-factor arrays; this refinement does not claim native target-gene repression, a specific repression motif, or an obligatory complex for every screen hit. The recovered full article supports this interaction-screen framework; its general statement that most partners are transcription factors is not used as evidence that every partner binds DNA. Exact annotated pairs are retained from the curated experimental record; the text extraction does not reproduce every supplemental pair table. Partner role, rather than the screen method, determines the replacement term.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with MIP1A (B-box domain protein 30 (AtBBX30) (Microprotein 1A)), reported by this study. This partner is a non-DNA-binding repressor or adaptor (e.g. NINJA, AFP, JAZ, KIX, microProtein or TWA1) that bridges TPL to transcription factors.

Reason: Generic protein binding is uninformative. Binding of a non-DNA-binding transcriptional co-regulator/adaptor is captured by transcription coregulator binding.

Proposed replacements: transcription coregulator binding

Summary: TPL is part of a repressor complex with HISTONE DEACETYLASE19 (and the adaptor MIF2/KNU) at the WUS locus.

Reason: Replace generic protein binding with histone deacetylase binding; TPL-HDA19 association is a recurring mechanism of TPL-mediated repression.

Proposed replacements: histone deacetylase binding

Summary: TPL interaction with the DNA-binding transcription factor SRG1/ZAT8 (At3g46080) (C2H2 zinc-finger transcription factor), reported by this study. TPL is recruited by EAR-motif or other repression-domain-containing transcription factors to repress their target genes.

Reason: Generic protein binding is uninformative. Direct binding of a DNA-binding transcription factor by the co-repressor is captured by DNA-binding transcription factor binding.

Proposed replacements: DNA-binding transcription factor binding

Summary: TPL interaction with NPR3 (Regulatory protein NPR3 (BTB/POZ domain-containing protein NPR3)), reported by this study. No functional consequence of this interaction for TPL-mediated repression has been established.

Reason: Generic protein binding is uninformative; no more specific function can be assigned from this interaction evidence alone. Removal does not imply the interaction is false.

Summary: TPL CRA-domain helix 8 directly binds the Mediator middle-module subunit MED21; this interaction is required for full repression.

Reason: Replace generic protein binding with mediator complex binding, which captures the mechanistic basis of TPL repression. The direct interaction and repression experiments combine an engineered Arabidopsis auxin circuit in yeast with plant assays; necessity for full repression is bounded to those constructs and conditions, not every endogenous TPL target.

Proposed replacements: mediator complex binding

Summary: TPL CRA-domain helix 8 directly binds the Mediator middle-module subunit MED10B; this interaction is required for full repression.

Reason: Replace generic protein binding with mediator complex binding, which captures the mechanistic basis of TPL repression. The direct interaction and repression experiments combine an engineered Arabidopsis auxin circuit in yeast with plant assays; necessity for full repression is bounded to those constructs and conditions, not every endogenous TPL target.

Proposed replacements: mediator complex binding

Summary: TPL associates with thermosensory coregulator TWA1 in the temperature-dependent JAM–TWA1–TPL/TPR repression system (PMID:38750356).

Reason: The study identifies a thermosensory transcriptional coregulator that assembles with JAM factors and TPL/TPRs; transcription coregulator binding captures this measured role without treating TWA1 as a DNA-binding factor.

Proposed replacements: transcription coregulator binding

Summary: Nuclear localization of TPL shown experimentally (UniProt curation).

Reason: Consistent with TPL co-repressor function.

Summary: Nuclear localization of TPL shown experimentally (UniProt curation).

Reason: Consistent with TPL co-repressor function.

Summary: Nuclear localization of TPL shown experimentally (UniProt curation).

Reason: Consistent with TPL co-repressor function.

Summary: Nuclear activity inferred for the TPL/TPR clade.

Reason: TPL is nuclear.

Summary: Nuclear localization of TPL shown experimentally.

Reason: TPL acts in the nucleus as a transcriptional co-repressor.

Summary: Nuclear localization of TPL shown experimentally.

Reason: TPL acts in the nucleus as a transcriptional co-repressor.

Summary: Nuclear localization from ARBA/UniProt subcellular mapping.

Reason: Consistent with experimental data.

Summary: Computational cytoplasmic localization remains unresolved; nuclear predominance is not evidence of absence from cytoplasm.

Reason: The AtSubP computational cytoplasm assignment cannot be independently resolved from the target evidence inspected. Strong nuclear localization supports the nuclear claims but does not exclude an additional cytoplasmic pool. No inaccessible experimental paper is invoked for this prediction-only row, and no contamination or exclusive-nucleus claim is inferred.

Summary: Regulation of DNA-templated transcription via the Topless family InterPro entry.

Reason: TPL is a demonstrated transcriptional corepressor; refine the true regulation parent to the negative direction already supported by direct repression assays and the existing negative-regulation annotations.

Proposed replacements: negative regulation of DNA-templated transcription

Summary: TPL binds the Aux/IAA IAA12/BDL and mediates auxin-dependent transcriptional repression during embryogenesis.

Reason: TPL is the co-repressor of the nuclear auxin pathway; involvement in auxin response is well supported (the IPI with IAA12 underpins it).

Summary: Jasmonic acid mediated signaling pathway, inferred for the TPL clade.

Reason: TPL is recruited via NINJA to JAZ repressors and negatively regulates jasmonate responses.

Summary: TPL loss/dominant-negative mutants show enhanced jasmonate responses; TPL is recruited to JAZ repressors via NINJA.

Reason: TPL is the co-repressor in JA signalling as well as auxin signalling.

Summary: tpl-1 suppresses the vascular patterning defects of bdl, placing TPL-mediated repression upstream of xylem/phloem pattern formation.

Reason: Developmental output of TPL-mediated repression of ARF5/MP via IAA12.

Summary: Primary shoot apical meristem specification, inferred for the TPL clade.

Reason: Supported by tpl-1 apical-to-root transformation; developmental output.

Summary: tpl-1 (dominant negative) transforms the embryonic shoot pole into a second root pole.

Reason: Developmental consequence of TPL co-repressor activity (repression of root-promoting genes such as PLT1/PLT2 in the apical embryo).

Summary: TPL self-interaction detected in the NINJA study (TPL/TPR oligomerization).

Reason: TPL self-association is a measured biochemical property of the corepressor, supported by structural and interaction evidence. Retain it as a core assembly property. PMID:34075876 shows that impaired multimerization has limited effects on repression in its engineered assays, so self-association is not described as universally required for every repressive output.

Summary: TPL self-interaction from the Arabidopsis Interactome-1 binary map.

Reason: TPL self-association is a measured biochemical property of the corepressor, supported by structural and interaction evidence. Retain it as a core assembly property. PMID:34075876 shows that impaired multimerization has limited effects on repression in its engineered assays, so self-association is not described as universally required for every repressive output.

Summary: TPL self-interaction (multimerization) examined in the Mediator study; multimer formation had minimal influence on repression strength.

Reason: TPL self-association is a measured biochemical property of the corepressor, supported by structural and interaction evidence. Retain it as a core assembly property. PMID:34075876 shows that impaired multimerization has limited effects on repression in its engineered assays, so self-association is not described as universally required for every repressive output.

Summary: TPL homodimerization detected in interaction assays.

Reason: TPL self-association is a measured biochemical property of the corepressor, supported by structural and interaction evidence. Retain it as a core assembly property. PMID:34075876 shows that impaired multimerization has limited effects on repression in its engineered assays, so self-association is not described as universally required for every repressive output.

Summary: TPL is required for the repressive activity of IAA12/BDL in vivo and acts as a transcriptional co-repressor of ARF5/MP-dependent transcription.

Reason: Direct evidence of the core repressive role.

Summary: Negative regulation of DNA-templated transcription via ARBA.

Reason: Correct; TPL represses transcription.