WRKY70 is an Arabidopsis group III WRKY DNA-binding transcription factor that acts in the nucleus to integrate salicylic-acid, jasmonic-acid, defense, and senescence transcriptional programs. Its core role is sequence-specific transcriptional regulation of target genes: it generally promotes SA-associated defense outputs, represses subsets of JA/ethylene-responsive defense genes, and cooperates with related WRKY factors as a negative regulator of developmental leaf senescence. Its many roles in pathogen, osmotic, stomatal, hormone, and metabolite responses are downstream, context-specific outputs of this transcriptional regulator rather than separate molecular functions.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0009759 indole glucosinolate biosynthetic process | IMP PMID:16623907 WRKY70 modulates the selection of signaling pathways in plan... | KEEP AS NON CORE | Summary: WRKY70 affects defense metabolite outputs as part of broader SA/JA defense transcriptional reprogramming. Reason: Indole glucosinolate, camalexin, and related metabolite changes are downstream defense outputs of WRKY70 regulation, not the core molecular function of the transcription factor. |
| GO:0009864 induced systemic resistance, jasmonic acid mediated signaling pathway | IMP PMID:16623907 WRKY70 modulates the selection of signaling pathways in plan... | MODIFY | Summary: The cited WRKY70 work supports modulation of JA-mediated defense signaling rather than a dedicated induced-systemic-resistance pathway. Reason: Use regulation of jasmonic acid mediated signaling pathway to capture the supported WRKY70 role in JA/SA pathway choice. Proposed replacements: regulation of jasmonic acid mediated signaling pathway |
| GO:0010120 camalexin biosynthetic process | IMP PMID:16623907 WRKY70 modulates the selection of signaling pathways in plan... | KEEP AS NON CORE | Summary: WRKY70 affects defense metabolite outputs as part of broader SA/JA defense transcriptional reprogramming. Reason: Indole glucosinolate, camalexin, and related metabolite changes are downstream defense outputs of WRKY70 regulation, not the core molecular function of the transcription factor. |
| GO:0009751 response to salicylic acid | IEP PMID:22268143 WRKY54 and WRKY70 co-operate as negative regulators of leaf ... | ACCEPT | Summary: WRKY70 is induced by SA and promotes SA-associated defense transcriptional outputs. Reason: SA-mediated transcriptional regulation is a central experimentally supported WRKY70 role. Supporting Evidence: PMID:22268143 RT-qPCR analysis of WRKY expression in wild-type and SA-deficient mutants suggests a common but not exclusive role for SA in induction of WRKY30, 53, 54, and 70 during senescence. |
| GO:0009753 response to jasmonic acid | IMP PMID:16623907 WRKY70 modulates the selection of signaling pathways in plan... | ACCEPT | Summary: WRKY70 modulates jasmonic-acid signaling, including repression of subsets of JA-responsive defense genes. Reason: Regulation of JA-mediated signaling is central to WRKY70 function as an SA/JA pathway-choice transcriptional node. |
| GO:0031347 regulation of defense response | IMP PMID:17096590 A genomic approach to identify regulatory nodes in the trans... | KEEP AS NON CORE | Summary: WRKY70 modulates pathogen defense outcomes through transcriptional control of hormone-responsive defense programs. Reason: The pathogen-response phenotype is supported, but it is a downstream biological output of WRKY70 transcriptional regulation rather than the core molecular function. |
| GO:0042742 defense response to bacterium | IMP PMID:16623907 WRKY70 modulates the selection of signaling pathways in plan... | KEEP AS NON CORE | Summary: WRKY70 modulates pathogen defense outcomes through transcriptional control of hormone-responsive defense programs. Reason: The pathogen-response phenotype is supported, but it is a downstream biological output of WRKY70 transcriptional regulation rather than the core molecular function. |
| GO:0045892 negative regulation of DNA-templated transcription | IMP PMID:16623907 WRKY70 modulates the selection of signaling pathways in plan... | ACCEPT | Summary: WRKY70 represses subsets of JA-responsive and other target genes as part of defense pathway crosstalk. Reason: Negative transcriptional regulation is directly supported for WRKY70 and is part of its core transcription-factor role. |
| GO:0050832 defense response to fungus | IMP PMID:16925600 Expression profiling and mutant analysis reveals complex reg... | KEEP AS NON CORE | Summary: WRKY70 modulates pathogen defense outcomes through transcriptional control of hormone-responsive defense programs. Reason: The pathogen-response phenotype is supported, but it is a downstream biological output of WRKY70 transcriptional regulation rather than the core molecular function. |
| GO:0071456 cellular response to hypoxia | HEP PMID:31519798 Integrative Analysis from the Epigenome to Translatome Uncov... | KEEP AS NON CORE | Summary: WRKY70 participates in stress or hormone-response contexts through transcriptional regulation. Reason: These stress, stomatal, brassinosteroid, ethylene, ROS, osmotic, and water-deprivation annotations are context-specific regulatory outputs rather than the core WRKY70 molecular function. |
| GO:1900056 negative regulation of leaf senescence | IGI PMID:22268143 WRKY54 and WRKY70 co-operate as negative regulators of leaf ... | ACCEPT | Summary: WRKY70 cooperates with WRKY54 as a negative regulator of developmental leaf senescence. Reason: Negative regulation of leaf senescence is a well-supported biological role of WRKY70. Supporting Evidence: PMID:22268143 WRKY54 and WRKY70 co-operate as negative regulators of leaf senescence in Arabidopsis thaliana. |
| GO:0009862 systemic acquired resistance, salicylic acid mediated signaling pathway | IMP PMID:16623907 WRKY70 modulates the selection of signaling pathways in plan... | KEEP AS NON CORE | Summary: WRKY70 promotes SA-associated systemic defense transcriptional outputs, but SAR is a downstream pathway outcome. Reason: The annotation is biologically consistent with WRKY70 defense crosstalk, but the core function is nuclear transcriptional regulation of SA/JA and senescence programs rather than SAR itself. |
| GO:0000976 transcription cis-regulatory region binding | IPI PMID:30356219 Transcriptional regulation of nitrogen-associated metabolism... | ACCEPT | Summary: WRKY70 binds transcriptional cis-regulatory regions in target promoters. Reason: Cis-regulatory binding is a direct molecular function of WRKY70 as a WRKY transcription factor. Supporting Evidence: PMID:24104863 YGACTTTT as WRKY70 binding site |
| GO:0003700 DNA-binding transcription factor activity | IEA GO_REF:0000002 | ACCEPT | Summary: WRKY70 is a DNA-binding WRKY transcription factor regulating defense and senescence gene expression programs. Reason: DNA-binding transcription factor activity is the core molecular function of WRKY70. Supporting Evidence: file:ARATH/WRKY70/WRKY70-deep-research-falcon.md WRKY70 encodes a **sequence-specific transcription factor** |
| GO:0003700 DNA-binding transcription factor activity | ISS PMID:10785665 The WRKY superfamily of plant transcription factors. | ACCEPT | Summary: WRKY70 is a DNA-binding WRKY transcription factor regulating defense and senescence gene expression programs. Reason: DNA-binding transcription factor activity is the core molecular function of WRKY70. |
| GO:0003700 DNA-binding transcription factor activity | ISS PMID:11118137 Arabidopsis transcription factors: genome-wide comparative a... | ACCEPT | Summary: WRKY70 is a DNA-binding WRKY transcription factor regulating defense and senescence gene expression programs. Reason: DNA-binding transcription factor activity is the core molecular function of WRKY70. |
| GO:0003700 DNA-binding transcription factor activity | IMP PMID:17096590 A genomic approach to identify regulatory nodes in the trans... | ACCEPT | Summary: WRKY70 is a DNA-binding WRKY transcription factor regulating defense and senescence gene expression programs. Reason: DNA-binding transcription factor activity is the core molecular function of WRKY70. Supporting Evidence: PMID:17096590 This large family of 74 transcription factors has been implicated in various defense responses |
| GO:0003700 DNA-binding transcription factor activity | IDA PMID:24104863 Identification of a novel type of WRKY transcription factor ... | ACCEPT | Summary: WRKY70 is a DNA-binding WRKY transcription factor regulating defense and senescence gene expression programs. Reason: DNA-binding transcription factor activity is the core molecular function of WRKY70. |
| GO:0005515 protein binding | IPI PMID:22268143 WRKY54 and WRKY70 co-operate as negative regulators of leaf ... | MARK AS OVER ANNOTATED | Summary: WRKY70 interacts with transcriptional and signaling regulators, but generic protein binding does not describe its functional role. Reason: The informative molecular function is DNA-binding transcription-factor activity and cis-regulatory binding; protein binding alone is too generic. |
| GO:0005515 protein binding | IPI PMID:26269953 Posttranslational Modifications of the Master Transcriptiona... | MARK AS OVER ANNOTATED | Summary: WRKY70 interacts with transcriptional and signaling regulators, but generic protein binding does not describe its functional role. Reason: The informative molecular function is DNA-binding transcription-factor activity and cis-regulatory binding; protein binding alone is too generic. |
| GO:0005515 protein binding | IPI PMID:28576847 Arabidopsis WRKY46, WRKY54, and WRKY70 Transcription Factors... | MARK AS OVER ANNOTATED | Summary: WRKY70 interacts with transcriptional and signaling regulators, but generic protein binding does not describe its functional role. Reason: The informative molecular function is DNA-binding transcription-factor activity and cis-regulatory binding; protein binding alone is too generic. |
| GO:0043565 sequence-specific DNA binding | IEA GO_REF:0000002 | ACCEPT | Summary: WRKY70 binds specific WRKY cis-elements in promoters of target genes. Reason: Sequence-specific DNA binding is a direct supported molecular function of WRKY70. |
| GO:0043565 sequence-specific DNA binding | IDA PMID:24104863 Identification of a novel type of WRKY transcription factor ... | ACCEPT | Summary: WRKY70 binds specific WRKY cis-elements in promoters of target genes. Reason: Sequence-specific DNA binding is a direct supported molecular function of WRKY70. |
| GO:0000302 response to reactive oxygen species | IEP PMID:22268143 WRKY54 and WRKY70 co-operate as negative regulators of leaf ... | KEEP AS NON CORE | Summary: WRKY70 participates in stress or hormone-response contexts through transcriptional regulation. Reason: These stress, stomatal, brassinosteroid, ethylene, ROS, osmotic, and water-deprivation annotations are context-specific regulatory outputs rather than the core WRKY70 molecular function. |
| GO:0002229 defense response to oomycetes | IMP PMID:17313163 Arabidopsis WRKY70 is required for full RPP4-mediated diseas... | KEEP AS NON CORE | Summary: WRKY70 modulates pathogen defense outcomes through transcriptional control of hormone-responsive defense programs. Reason: The pathogen-response phenotype is supported, but it is a downstream biological output of WRKY70 transcriptional regulation rather than the core molecular function. |
| GO:0006355 regulation of DNA-templated transcription | IEA GO_REF:0000002 | ACCEPT | Summary: WRKY70 regulates target-gene transcription in defense, hormone, nitrogen, and senescence contexts. Reason: Regulation of DNA-templated transcription is core to WRKY70 function, although the molecular function is better captured by DNA-binding transcription factor activity. |
| GO:0006355 regulation of DNA-templated transcription | IMP PMID:17313163 Arabidopsis WRKY70 is required for full RPP4-mediated diseas... | ACCEPT | Summary: WRKY70 regulates target-gene transcription in defense, hormone, nitrogen, and senescence contexts. Reason: Regulation of DNA-templated transcription is core to WRKY70 function, although the molecular function is better captured by DNA-binding transcription factor activity. |
| GO:0006970 response to osmotic stress | IEP PMID:23815736 Defense-related transcription factors WRKY70 and WRKY54 modu... | KEEP AS NON CORE | Summary: WRKY70 participates in stress or hormone-response contexts through transcriptional regulation. Reason: These stress, stomatal, brassinosteroid, ethylene, ROS, osmotic, and water-deprivation annotations are context-specific regulatory outputs rather than the core WRKY70 molecular function. |
| GO:0009617 response to bacterium | IEP PMID:14742872 The WRKY70 transcription factor: a node of convergence for j... | KEEP AS NON CORE | Summary: The cited expression evidence places WRKY70 in bacterial-response contexts, while stronger mutant evidence separately supports bacterial defense roles. Reason: Response to bacterium is a supported expression/pathogen-response output, but WRKY70's core function is transcriptional regulation and this IEP row should not be upgraded to a defense-response term. |
| GO:0009617 response to bacterium | IEP PMID:17965588 Epigenetic control of a transcription factor at the cross se... | KEEP AS NON CORE | Summary: The cited expression evidence places WRKY70 in bacterial-response contexts, while stronger mutant evidence separately supports bacterial defense roles. Reason: Response to bacterium is a supported expression/pathogen-response output, but WRKY70's core function is transcriptional regulation and this IEP row should not be upgraded to a defense-response term. |
| GO:0009617 response to bacterium | IEP PMID:22325892 Arabidopsis WRKY46 coordinates with WRKY70 and WRKY53 in bas... | KEEP AS NON CORE | Summary: The cited expression evidence places WRKY70 in bacterial-response contexts, while stronger mutant evidence separately supports bacterial defense roles. Reason: Response to bacterium is a supported expression/pathogen-response output, but WRKY70's core function is transcriptional regulation and this IEP row should not be upgraded to a defense-response term. |
| GO:0009617 response to bacterium | IEP PMID:26433201 Transcription factors WRKY70 and WRKY11 served as regulators... | KEEP AS NON CORE | Summary: The cited expression evidence places WRKY70 in bacterial-response contexts, while stronger mutant evidence separately supports bacterial defense roles. Reason: Response to bacterium is a supported expression/pathogen-response output, but WRKY70's core function is transcriptional regulation and this IEP row should not be upgraded to a defense-response term. |
| GO:0009625 response to insect | IEP PMID:25339349 Density-dependent interference of aphids with caterpillar-in... | KEEP AS NON CORE | Summary: WRKY70 is implicated in insect-response contexts, but this is a specialized output. Reason: Insect response is not the central WRKY70 role; it is a context-specific downstream defense phenotype. |
| GO:0009682 induced systemic resistance | IMP PMID:26433201 Transcription factors WRKY70 and WRKY11 served as regulators... | KEEP AS NON CORE | Summary: WRKY70 modulates pathogen defense outcomes through transcriptional control of hormone-responsive defense programs. Reason: The pathogen-response phenotype is supported, but it is a downstream biological output of WRKY70 transcriptional regulation rather than the core molecular function. |
| GO:0009751 response to salicylic acid | IEP PMID:14742872 The WRKY70 transcription factor: a node of convergence for j... | ACCEPT | Summary: WRKY70 is induced by SA and promotes SA-associated defense transcriptional outputs. Reason: SA-mediated transcriptional regulation is a central experimentally supported WRKY70 role. Supporting Evidence: PMID:14742872 We suggest that WRKY70 acts as an activator of SA-induced genes and a repressor of JA-responsive genes |
| GO:0009751 response to salicylic acid | IEP PMID:22325892 Arabidopsis WRKY46 coordinates with WRKY70 and WRKY53 in bas... | ACCEPT | Summary: WRKY70 is induced by SA and promotes SA-associated defense transcriptional outputs. Reason: SA-mediated transcriptional regulation is a central experimentally supported WRKY70 role. |
| GO:0009751 response to salicylic acid | IEP PMID:26433201 Transcription factors WRKY70 and WRKY11 served as regulators... | ACCEPT | Summary: WRKY70 is induced by SA and promotes SA-associated defense transcriptional outputs. Reason: SA-mediated transcriptional regulation is a central experimentally supported WRKY70 role. |
| GO:0009753 response to jasmonic acid | IEP PMID:14742872 The WRKY70 transcription factor: a node of convergence for j... | ACCEPT | Summary: WRKY70 modulates jasmonic-acid signaling, including repression of subsets of JA-responsive defense genes. Reason: Regulation of JA-mediated signaling is central to WRKY70 function as an SA/JA pathway-choice transcriptional node. Supporting Evidence: PMID:14742872 We suggest that WRKY70 acts as an activator of SA-induced genes and a repressor of JA-responsive genes |
| GO:0010104 regulation of ethylene-activated signaling pathway | IMP PMID:28837631 WRKY70 and its homolog WRKY54 negatively modulate the cell w... | KEEP AS NON CORE | Summary: WRKY70 participates in stress or hormone-response contexts through transcriptional regulation. Reason: These stress, stomatal, brassinosteroid, ethylene, ROS, osmotic, and water-deprivation annotations are context-specific regulatory outputs rather than the core WRKY70 molecular function. |
| GO:0010150 leaf senescence | IEP PMID:17310369 The WRKY70 transcription factor of Arabidopsis influences bo... | MODIFY | Summary: WRKY70 affects leaf senescence primarily as a negative regulator rather than merely as a participant in senescence. Reason: Use the regulatory term to capture the direction of WRKY70 action in leaf senescence. Proposed replacements: negative regulation of leaf senescence |
| GO:0010150 leaf senescence | IMP PMID:17310369 The WRKY70 transcription factor of Arabidopsis influences bo... | MODIFY | Summary: WRKY70 affects leaf senescence primarily as a negative regulator rather than merely as a participant in senescence. Reason: Use the regulatory term to capture the direction of WRKY70 action in leaf senescence. Proposed replacements: negative regulation of leaf senescence |
| GO:0031347 regulation of defense response | IMP PMID:14742872 The WRKY70 transcription factor: a node of convergence for j... | KEEP AS NON CORE | Summary: WRKY70 modulates pathogen defense outcomes through transcriptional control of hormone-responsive defense programs. Reason: The pathogen-response phenotype is supported, but it is a downstream biological output of WRKY70 transcriptional regulation rather than the core molecular function. |
| GO:0042742 defense response to bacterium | IMP PMID:22325892 Arabidopsis WRKY46 coordinates with WRKY70 and WRKY53 in bas... | KEEP AS NON CORE | Summary: WRKY70 modulates pathogen defense outcomes through transcriptional control of hormone-responsive defense programs. Reason: The pathogen-response phenotype is supported, but it is a downstream biological output of WRKY70 transcriptional regulation rather than the core molecular function. |
| GO:0042742 defense response to bacterium | IMP PMID:26433201 Transcription factors WRKY70 and WRKY11 served as regulators... | KEEP AS NON CORE | Summary: WRKY70 modulates pathogen defense outcomes through transcriptional control of hormone-responsive defense programs. Reason: The pathogen-response phenotype is supported, but it is a downstream biological output of WRKY70 transcriptional regulation rather than the core molecular function. |
| GO:0042742 defense response to bacterium | IMP PMID:28837631 WRKY70 and its homolog WRKY54 negatively modulate the cell w... | KEEP AS NON CORE | Summary: WRKY70 modulates pathogen defense outcomes through transcriptional control of hormone-responsive defense programs. Reason: The pathogen-response phenotype is supported, but it is a downstream biological output of WRKY70 transcriptional regulation rather than the core molecular function. |
| GO:0047484 regulation of response to osmotic stress | IMP PMID:23815736 Defense-related transcription factors WRKY70 and WRKY54 modu... | KEEP AS NON CORE | Summary: WRKY70 participates in stress or hormone-response contexts through transcriptional regulation. Reason: These stress, stomatal, brassinosteroid, ethylene, ROS, osmotic, and water-deprivation annotations are context-specific regulatory outputs rather than the core WRKY70 molecular function. |
| GO:0050832 defense response to fungus | IMP PMID:16623907 WRKY70 modulates the selection of signaling pathways in plan... | KEEP AS NON CORE | Summary: WRKY70 modulates pathogen defense outcomes through transcriptional control of hormone-responsive defense programs. Reason: The pathogen-response phenotype is supported, but it is a downstream biological output of WRKY70 transcriptional regulation rather than the core molecular function. |
| GO:0050832 defense response to fungus | IMP PMID:28837631 WRKY70 and its homolog WRKY54 negatively modulate the cell w... | KEEP AS NON CORE | Summary: WRKY70 modulates pathogen defense outcomes through transcriptional control of hormone-responsive defense programs. Reason: The pathogen-response phenotype is supported, but it is a downstream biological output of WRKY70 transcriptional regulation rather than the core molecular function. |
| GO:0080151 positive regulation of salicylic acid mediated signaling pathway | IMP PMID:14742872 The WRKY70 transcription factor: a node of convergence for j... | ACCEPT | Summary: WRKY70 is induced by SA and promotes SA-associated defense transcriptional outputs. Reason: SA-mediated transcriptional regulation is a central experimentally supported WRKY70 role. Supporting Evidence: PMID:14742872 We suggest that WRKY70 acts as an activator of SA-induced genes and a repressor of JA-responsive genes |
| GO:0080187 floral organ senescence | IEP PMID:17310369 The WRKY70 transcription factor of Arabidopsis influences bo... | KEEP AS NON CORE | Summary: WRKY70 accumulates in floral senescence contexts, but the central developmental role is leaf-senescence regulation. Reason: Floral organ senescence is a context-specific developmental expression/output annotation rather than the core WRKY70 function. |
| GO:0090333 regulation of stomatal closure | IMP PMID:23815736 Defense-related transcription factors WRKY70 and WRKY54 modu... | KEEP AS NON CORE | Summary: WRKY70 participates in stress or hormone-response contexts through transcriptional regulation. Reason: These stress, stomatal, brassinosteroid, ethylene, ROS, osmotic, and water-deprivation annotations are context-specific regulatory outputs rather than the core WRKY70 molecular function. |
| GO:1900457 regulation of brassinosteroid mediated signaling pathway | IMP PMID:28576847 Arabidopsis WRKY46, WRKY54, and WRKY70 Transcription Factors... | KEEP AS NON CORE | Summary: WRKY70 participates in stress or hormone-response contexts through transcriptional regulation. Reason: These stress, stomatal, brassinosteroid, ethylene, ROS, osmotic, and water-deprivation annotations are context-specific regulatory outputs rather than the core WRKY70 molecular function. |
| GO:2000022 regulation of jasmonic acid mediated signaling pathway | IMP PMID:14742872 The WRKY70 transcription factor: a node of convergence for j... | ACCEPT | Summary: WRKY70 modulates jasmonic-acid signaling, including repression of subsets of JA-responsive defense genes. Reason: Regulation of JA-mediated signaling is central to WRKY70 function as an SA/JA pathway-choice transcriptional node. Supporting Evidence: PMID:14742872 We suggest that WRKY70 acts as an activator of SA-induced genes and a repressor of JA-responsive genes |
| GO:2000022 regulation of jasmonic acid mediated signaling pathway | IMP PMID:22325892 Arabidopsis WRKY46 coordinates with WRKY70 and WRKY53 in bas... | ACCEPT | Summary: WRKY70 modulates jasmonic-acid signaling, including repression of subsets of JA-responsive defense genes. Reason: Regulation of JA-mediated signaling is central to WRKY70 function as an SA/JA pathway-choice transcriptional node. |
| GO:2000022 regulation of jasmonic acid mediated signaling pathway | IMP PMID:28837631 WRKY70 and its homolog WRKY54 negatively modulate the cell w... | ACCEPT | Summary: WRKY70 modulates jasmonic-acid signaling, including repression of subsets of JA-responsive defense genes. Reason: Regulation of JA-mediated signaling is central to WRKY70 function as an SA/JA pathway-choice transcriptional node. Supporting Evidence: PMID:28837631 up-regulated expression of both SA and JA/ethylene (ET) responsive defense related genes |
| GO:2000031 regulation of salicylic acid mediated signaling pathway | IDA PMID:26433201 Transcription factors WRKY70 and WRKY11 served as regulators... | ACCEPT | Summary: WRKY70 is induced by SA and promotes SA-associated defense transcriptional outputs. Reason: SA-mediated transcriptional regulation is a central experimentally supported WRKY70 role. |
| GO:2000031 regulation of salicylic acid mediated signaling pathway | IMP PMID:28837631 WRKY70 and its homolog WRKY54 negatively modulate the cell w... | ACCEPT | Summary: WRKY70 is induced by SA and promotes SA-associated defense transcriptional outputs. Reason: SA-mediated transcriptional regulation is a central experimentally supported WRKY70 role. Supporting Evidence: PMID:28837631 up-regulated expression of both SA and JA/ethylene (ET) responsive defense related genes |
| GO:2000070 regulation of response to water deprivation | IMP PMID:28576847 Arabidopsis WRKY46, WRKY54, and WRKY70 Transcription Factors... | KEEP AS NON CORE | Summary: WRKY70 participates in stress or hormone-response contexts through transcriptional regulation. Reason: These stress, stomatal, brassinosteroid, ethylene, ROS, osmotic, and water-deprivation annotations are context-specific regulatory outputs rather than the core WRKY70 molecular function. |
| GO:0005634 nucleus | IEA GO_REF:0000044 | ACCEPT | Summary: WRKY70 functions in the nucleus as a DNA-binding transcription factor. Reason: Nuclear localization is expected and supported for WRKY70 transcriptional regulation. |
| GO:0005634 nucleus | ISM GO_REF:0000122 | ACCEPT | Summary: WRKY70 functions in the nucleus as a DNA-binding transcription factor. Reason: Nuclear localization is expected and supported for WRKY70 transcriptional regulation. |
| GO:0005634 nucleus | IDA PMID:17310369 The WRKY70 transcription factor of Arabidopsis influences bo... | ACCEPT | Summary: WRKY70 functions in the nucleus as a DNA-binding transcription factor. Reason: Nuclear localization is expected and supported for WRKY70 transcriptional regulation. |
| GO:0005634 nucleus | IDA PMID:26433201 Transcription factors WRKY70 and WRKY11 served as regulators... | ACCEPT | Summary: WRKY70 functions in the nucleus as a DNA-binding transcription factor. Reason: Nuclear localization is expected and supported for WRKY70 transcriptional regulation. |
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Download this section (compressed HTML)Q: Should WRKY70 defense-output annotations be narrowed upstream to regulatory terms that capture its transcription-factor role and direction of regulation?
Q: Can direct AtWRKY70 chromatin-binding datasets distinguish primary targets from downstream defense and senescence phenotypes?
Q: Is the ICS1/SID2 de-repression seen in wrky54 wrky70 a direct effect of WRKY70 at the ICS1 promoter or an indirect one, given that in vitro DAP-seq detects no WRKY70 peak near ICS1 (or PR1) while several other WRKY proteins bind the ICS1 promoter?
Experiment: Perform WRKY70 ChIP-seq or CUT&RUN under SA, JA, and senescence conditions and pair it with rapid WRKY70 perturbation RNA-seq to distinguish direct targets from downstream outputs.
Experiment: Test WRKY70 DNA-binding and activation/repression activity on WT-box, W-box, PR, JA-responsive, and senescence-associated promoters using mutant binding motifs and WRKY70 DNA-binding-domain variants.
Experiment: Test in vivo WRKY70 occupancy at the ICS1/SID2 and PR1 promoter W-box/WT-box regions by ChIP-qPCR (tagged WRKY70 complementing wrky70) under mock, SA and pathogen treatment, including NPR1 sumoylation-site mutant backgrounds, to decide whether the WRKY70 -| SA-biosynthesis feedback is a direct promoter interaction.
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