id: A0A2U1PS28
gene_symbol: A0A2U1PS28
taxon:
  id: NCBITaxon:35608
  label: Artemisia annua
status: COMPLETE
description: >-
  The plant EF4 protein is assigned to a chloroplastic GUF1/cpLEPA subfamily, supporting chloroplast localization
  by family transfer.
source_documents:
  - genes/ARTAN/A0A2U1PS28/A0A2U1PS28-uniprot.txt
  - genes/ARTAN/A0A2U1PS28/A0A2U1PS28-goa.tsv
  - publications/PMID_23166764.md
  - genes/ARTAN/A0A2U1PS28/A0A2U1PS28-hypotheses/prediction-organellar-localization/openscientist.md
predictions:
  - source_method: ProtNLM2
    source_version: UniProt 2024_06 pilot
    predicted_term:
      id: GO:0009507
      label: chloroplast
    predicted_term_type: GO_CC
    review:
      assessment: COR
      confidence_score: 2
      summary: >-
        The 661-residue target has the EF4/LepA domain architecture and is assigned to the chloroplastic
        GUF1 subfamily PTHR43512:SF4 in the cached sequence record. Arabidopsis cpLEPA was experimentally
        characterized as a chloroplast translation factor (PMID:23166764), providing biological grounding
        for transfer of this localization within the plant subfamily. This is an inference from subfamily
        placement, not a targeting assay on Artemisia. The chloroplast term is absent from the cached
        annotations; the mitochondrial UniRule assignments are a conflicting automated inference rather
        than decisive localization evidence.
      supported_by:
        - reference_id: file:ARTAN/A0A2U1PS28/A0A2U1PS28-uniprot.txt
          supporting_text: >-
            ID   A0A2U1PS28_ARTAN        Unreviewed;       661 AA. ... DR   InterPro; IPR006297; EF-4.
            ... DR   PANTHER; PTHR43512:SF4; TRANSLATION FACTOR GUF1 HOMOLOG, CHLOROPLASTIC; 1. ... FT   DOMAIN          86..245
            ... FT                   /note="Tr-type G"
        - reference_id: PMID:23166764
          supporting_text: >-
            promotes the efficiency of chloroplast protein synthesis
        - reference_id: file:ARTAN/A0A2U1PS28/A0A2U1PS28-hypotheses/prediction-organellar-localization/openscientist.md
          supporting_text: >-
            the ProtNLM2 chloroplast prediction is the better-supported localization
references:
  - id: file:ARTAN/A0A2U1PS28/A0A2U1PS28-uniprot.txt
    title: A0A2U1PS28-uniprot.txt
  - id: PMID:23166764
    title: CpLEPA is critical for chloroplast protein synthesis under suboptimal conditions in
      Arabidopsis thaliana.
  - id: file:ARTAN/A0A2U1PS28/A0A2U1PS28-hypotheses/prediction-organellar-localization/openscientist.md
    title: openscientist.md