erg8 (AFUA_5G10680) encodes the fungal GHMP-type phosphomevalonate kinase that phosphorylates (R)-5-phosphomevalonate with ATP to form (R)-5-diphosphomevalonate. This is the second kinase step of isopentenyl diphosphate formation from mevalonate and supplies prenyl diphosphate precursors for ergosterol biosynthesis in Aspergillus fumigatus.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0004631 phosphomevalonate kinase activity | IBA GO_REF:0000033 | ACCEPT | Summary: IBA from PTN001274235, the fungal/plant GHMP phosphomevalonate kinase node seeded by Arabidopsis PMK and S. cerevisiae ERG8. Q4WV38 is in fungal PTHR31814:SF2, and the same phosphomevalonate kinase reaction is curated on the A. fumigatus Swiss-Prot record, so this is the core molecular function. Supporting Evidence: file:interpro/panther/PTHR31814/PTHR31814-paint.tsv PTHR31814 PTN001274235 GO:0004631 F IBD false file:ASPFU/erg8/erg8-uniprot.txt Reaction=(R)-5-phosphomevalonate + ATP = (R)-5-diphosphomevalonate + |
| GO:0004631 phosphomevalonate kinase activity | IEA GO_REF:0000120 | ACCEPT | Summary: Combined automated annotation from the yeast ERG8 ortholog, Erg8 InterPro signature, RHEA:16341, and EC:2.7.4.2. These all match the curated Q4WV38 phosphomevalonate kinase reaction. Supporting Evidence: file:ASPFU/erg8/erg8-uniprot.txt Reaction=(R)-5-phosphomevalonate + ATP = (R)-5-diphosphomevalonate + file:ASPFU/erg8/erg8-uniprot.txt DR InterPro; IPR035102; Phosphomevalonate_kinase. |
| GO:0005524 ATP binding | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: InterPro2GO from the GHMP kinase N-terminal domain. ATP is the phosphoryl donor in the Erg8 phosphomevalonate kinase reaction, so the binding term is correct but subsidiary to the kinase molecular function. Reason: ATP binding is substrate binding implied by the accepted phosphomevalonate kinase activity. Supporting Evidence: file:ASPFU/erg8/erg8-uniprot.txt DR InterPro; IPR006204; GHMP_kinase_N_dom. file:ASPFU/erg8/erg8-uniprot.txt Reaction=(R)-5-phosphomevalonate + ATP = (R)-5-diphosphomevalonate + |
| GO:0005777 peroxisome | IBA GO_REF:0000033 | REMOVE | Summary: IBA from PTN000781523, a plant peroxisomal PMK node seeded only by Arabidopsis AT1G31910. The PTHR31814 family review already marks this peroxisome node as needing pruning for the fungal PMK subfamily. Reason: PTN000781523 propagates a plant-lineage compartment to fungal Erg8 proteins; this is a compartment and taxon mismatch, not evidence that A. fumigatus erg8 acts in peroxisomes. Propagation Review Root cause: PROPAGATION BAD Failure modes: COMPARTMENT OR COMPLEX MISMATCH LINEAGE OR TAXON MISMATCH Sources checked: PANTHER:PTN000781523 · PTN000781523 SUPPORTS SOURCE BUT NOT TARGET The Arabidopsis-seeded peroxisome node should not propagate to fungal PTHR31814:SF2 Erg8 proteins. Supporting Evidence: file:interpro/panther/PTHR31814/PTHR31814-paint.tsv PTHR31814 PTN000781523 GO:0005777 C IBD false file:interpro/panther/PTHR31814/PTHR31814-review.yaml Peroxisome is removed by both the S. cerevisiae ERG8 and S. pombe erg8 reviews |
| GO:0006696 ergosterol biosynthetic process | IBA GO_REF:0000033 | ACCEPT | Summary: IBA from fungal node PTN001274236, seeded by yeast ERG8. A. fumigatus Erg8 produces the mevalonate-pathway intermediate that is converted to isopentenyl diphosphate and farnesyl diphosphate, a direct precursor route for fungal ergosterol. Supporting Evidence: file:interpro/panther/PTHR31814/PTHR31814-paint.tsv PTHR31814 PTN001274236 GO:0006696 P IBD false file:ASPFU/erg8/erg8-uniprot.txt Phosphomevalonate kinase; part of the second module of ergosterol biosynthesis pathway |
| GO:0006696 ergosterol biosynthetic process | IEA GO_REF:0000107 | ACCEPT | Summary: Ensembl Compara transfer from yeast ERG8 to the A. fumigatus erg8 ortholog. The transferred ergosterol-biosynthesis process is consistent with the curated PMK reaction and fungal PAINT node. Supporting Evidence: file:interpro/panther/PTHR31814/PTHR31814-paint.tsv PTHR31814 PTN001274236 GO:0006696 P IBD false file:ASPFU/erg8/erg8-uniprot.txt Phosphomevalonate kinase; part of the second module of ergosterol biosynthesis pathway |
| GO:0019287 isopentenyl diphosphate biosynthetic process, mevalonate pathway | IBA GO_REF:0000033 | ACCEPT | Summary: IBA from PTN001274235. The phosphomevalonate kinase reaction is step 2/3 in the UniProt-curated isopentenyl diphosphate biosynthetic route from mevalonate, so this is a direct core biological process for Erg8. Supporting Evidence: file:interpro/panther/PTHR31814/PTHR31814-paint.tsv PTHR31814 PTN001274235 GO:0019287 P IBD false file:ASPFU/erg8/erg8-uniprot.txt via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: |
| GO:0019287 isopentenyl diphosphate biosynthetic process, mevalonate pathway | IEA GO_REF:0000120 | ACCEPT | Summary: Combined automated annotation from the yeast ortholog and UniPathway UPA00057. This matches the Q4WV38 pathway statement that Erg8 catalyzes the second step of IPP biosynthesis from mevalonate. Supporting Evidence: file:ASPFU/erg8/erg8-uniprot.txt via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: file:ASPFU/erg8/erg8-uniprot.txt Reaction=(R)-5-phosphomevalonate + ATP = (R)-5-diphosphomevalonate + |
Loading supporting content…
Download this section (compressed HTML)Loading supporting content…
Download this section (compressed HTML)Loading supporting content…
Download this section (compressed HTML)