id: Q04720 gene_symbol: ermJ product_type: PROTEIN status: DRAFT taxon: id: NCBITaxon:1392 label: Bacillus anthracis description: ermJ encodes an Erm-family 23S rRNA adenine-N6 methyltransferase in Bacillus anthracis. UniProt/CARD identify this entry as a macrolide-lincosamide-streptogramin resistance determinant; methylation of 23S rRNA alters the macrolide binding site on the large ribosomal subunit. UniProt names the protein 'rRNA adenine N-6-methyltransferase' (accession Q04720). reference_proteome_assessments: - reference_proteomes: - UP000000427 - UP000000594 status: NO_SUPPORTED_COUNTERPART reviewed_on: '2026-10-19' reason: Retain Q04720 as the primary Swiss-Prot accession. Both exact-taxon reference proteomes were searched. The only hit is RsmA Q81W00 at 27.30% global identity, a distinct ribosomal methyltransferase. Related methyltransferase ancestry does not establish an ErmJ resistance counterpart; no supported alternative was identified. evidence: - type: PROTEOME_SEARCH description: MMseqs2 searched 10,161 sequences across UP000000427, UP000000594 at E <= 1e-5 and sensitivity 7.5, returning 1 hits. Every returned sequence was globally aligned using BLOSUM62 with gap-open -10 and extension -0.5. Scores and sequences for all 1 returned candidates are retained, with 1 detailed alignment files. The heuristic search does not establish genomic absence or unique locus identity. supported_by: - reference_id: file:BACAN/ermJ/ermJ-record-evidence.yaml supporting_text: 'target_sequence_count: 10161' - reference_id: file:BACAN/ermJ/ermJ-record-evidence.yaml supporting_text: 'candidate_count: 1' - type: PROTEOME_MEMBERSHIP description: The primary accession is outside every searched exact-taxon reference proteome. supported_by: - reference_id: file:BACAN/ermJ/ermJ-record-evidence.yaml supporting_text: 'primary_in_any_reference_proteome: false' - type: RECORD_HISTORY description: 'Sampled UniSave observations: 07-JAN-2015, entry version 65.: no Proteomes cross-reference; 11-DEC-2019, entry version 75.: no Proteomes cross-reference; 02-OCT-2024, entry version 84.: no Proteomes cross-reference. These dated observations do not establish uninterrupted membership/absence, the exact change date, or UniProt''s motivation.' supported_by: - reference_id: file:BACAN/ermJ/ermJ-record-evidence.yaml supporting_text: |- entry_date: 07-JAN-2015, entry version 65. proteomes: [] - reference_id: file:BACAN/ermJ/ermJ-record-evidence.yaml supporting_text: |- entry_date: 11-DEC-2019, entry version 75. proteomes: [] - reference_id: file:BACAN/ermJ/ermJ-record-evidence.yaml supporting_text: |- entry_date: 02-OCT-2024, entry version 84. proteomes: [] existing_annotations: - term: id: GO:0000154 label: rRNA modification evidence_type: IEA original_reference_id: GO_REF:0000002 qualifier: involved_in review: summary: The rRNA modification/methylation process is consistent with an Erm-family 23S rRNA methyltransferase. action: ACCEPT reason: Erm enzymes confer resistance by methylating 23S rRNA; this process annotation is biologically appropriate. supported_by: - reference_id: file:genes/BACAN/ermJ/ermJ-uniprot.txt record_section: ["CC:FUNCTION"] supporting_text: 'CC -!- FUNCTION: Involved in erythromycin resistance.' - term: id: GO:0000179 label: rRNA (adenine-N6,N6-)-dimethyltransferase activity evidence_type: IEA original_reference_id: GO_REF:0000120 qualifier: enables review: summary: The existing dimethyltransferase term is retained as the more specific molecular function for this Erm-family enzyme. action: ACCEPT reason: GO:0000179 is a child of the broader rRNA adenine-N6 methyltransferase activity and captures the N6,N6-dimethylation activity expected for Erm-mediated macrolide-lincosamide-streptogramin resistance. supported_by: - reference_id: file:genes/BACAN/ermJ/ermJ-uniprot.txt record_section: ["CC:FUNCTION"] supporting_text: 'CC -!- FUNCTION: Involved in erythromycin resistance.' - term: id: GO:0031167 label: rRNA methylation evidence_type: IEA original_reference_id: GO_REF:0000118 qualifier: involved_in review: summary: The rRNA modification/methylation process is consistent with an Erm-family 23S rRNA methyltransferase. action: ACCEPT reason: Erm enzymes confer resistance by methylating 23S rRNA; this process annotation is biologically appropriate. supported_by: - reference_id: file:genes/BACAN/ermJ/ermJ-uniprot.txt record_section: ["CC:FUNCTION"] supporting_text: 'CC -!- FUNCTION: Involved in erythromycin resistance.' - term: id: GO:0046677 label: response to antibiotic evidence_type: RCA original_reference_id: file:projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml qualifier: involved_in review: summary: 'NEW process annotation: ermJ is an AMR determinant involved in antibiotic response/resistance.' action: NEW reason: CARD/ARO identity places this gene in an antimicrobial-resistance determinant family; response to antibiotic is the appropriate high-level GO biological process for the resistance role. additional_reference_ids: - file:genes/BACAN/ermJ/ermJ-uniprot.txt supported_by: - reference_id: file:genes/BACAN/ermJ/ermJ-uniprot.txt record_section: ["DR:CARD"] supporting_text: DR CARD; ARO:3000495; ErmD; ARO:0001001; antibiotic target alteration. references: - id: file:BACAN/ermJ/ermJ-record-evidence.yaml title: Compact archived record and sequence evidence for Q04720 - id: GO_REF:0000002 title: Gene Ontology annotation through association of InterPro records with GO terms findings: [] - id: GO_REF:0000118 title: TreeGrafter-generated GO annotations findings: [] - id: GO_REF:0000120 title: Combined Automated Annotation using Multiple IEA Methods findings: [] - id: file:genes/BACAN/ermJ/ermJ-uniprot.txt title: UniProt flat file for ermJ (Q04720) findings: - statement: UniProt/CARD identity and protein naming for ermJ. record_section: ["CC:FUNCTION"] supporting_text: 'CC -!- FUNCTION: Involved in erythromycin resistance.' - id: file:projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml title: Curated ARO to GO mapping set for AMR gene families findings: - statement: Manual ARO-to-GO mappings used to identify focused AMR annotation gains. supporting_text: 'mapping_set_title: ARO to GO mapping (AMR gene families and resistance mechanisms)' - id: file:projects/ANTIMICROBIAL_RESISTANCE/data/candidate_new_annotations.tsv title: Annotation-gain candidates from ARO to GO mappings findings: - statement: Candidate GO annotations projected from the ARO-to-GO mapping set. supporting_text: Candidate GO annotations that UniProtKB entries with a CARD cross-reference would gain from the core_functions: - description: ermJ methylates adenine N6 in 23S rRNA, modifying the antibiotic target site in the large ribosomal subunit and contributing to macrolide/lincosamide/streptogramin resistance. molecular_function: id: GO:0000179 label: rRNA (adenine-N6,N6-)-dimethyltransferase activity directly_involved_in: - id: GO:0046677 label: response to antibiotic supported_by: - reference_id: file:genes/BACAN/ermJ/ermJ-uniprot.txt record_section: ["CC:FUNCTION"] supporting_text: 'CC -!- FUNCTION: Involved in erythromycin resistance.' suggested_questions: - question: Is the ARO-derived rRNA (adenine-N6-)-methyltransferase activity annotation sufficiently specific for ermJ, or is a narrower substrate/site-specific GO term warranted? experts: [] suggested_experiments: - description: Biochemically assay purified ermJ against representative antibiotic substrates for the inferred AMR family and measure loss of drug activity or target modification. experiment_type: in vitro enzyme assay