knt

UniProt ID: P05058
Organism: Bacillus sp
Review Status: DRAFT
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Gene Description

knt encodes an aminoglycoside nucleotidyltransferase in Bacillus sp. UniProt/CARD identify this entry as an aminoglycoside-modifying resistance enzyme that inactivates aminoglycoside antibiotics by adenylylation/nucleotidyl transfer. UniProt names the protein 'Aminoglycoside nucleotidyltransferase (4')' (accession P05058).

Existing Annotations Review

GO Term Evidence Action Reason
GO:0016779 nucleotidyltransferase activity
IEA
GO_REF:0000002 		MODIFY
Summary: The existing MF term is directionally correct but less informative than the family-specific AMR activity aminoglycoside nucleotidyltransferase activity.
Reason: Replace the broad or over-specific electronic term 'nucleotidyltransferase activity' with aminoglycoside nucleotidyltransferase activity based on UniProt/CARD determinant identity and the curated ARO->GO mapping.
Supporting Evidence:
file:genes/BACSP/knt/knt-uniprot.txt
CC -!- FUNCTION: Inactivates aminoglycoside antibiotics such as kanamycin by CC catalyzing the transfer of a nucleotidyl group from nucleoside CC triphosphates such as (d)ATP to the 4'-hydroxyl group of the
GO:0046677 response to antibiotic
IEA
GO_REF:0000002 		ACCEPT
Summary: The gene product is an antimicrobial-resistance determinant, so the antibiotic-response annotation is retained.
Reason: The CARD/ARO determinant identity supports an antibiotic-resistance biological role.
Supporting Evidence:
file:genes/BACSP/knt/knt-uniprot.txt
CC -!- FUNCTION: Inactivates aminoglycoside antibiotics such as kanamycin by CC catalyzing the transfer of a nucleotidyl group from nucleoside CC triphosphates such as (d)ATP to the 4'-hydroxyl group of the
GO:0034068 aminoglycoside nucleotidyltransferase activity
RCA
file:projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml 		NEW
Summary: NEW candidate annotation from the curated ARO->GO mapping: aminoglycoside nucleotidyltransferase activity.
Reason: The UniProt record cross-references a CARD/ARO AMR determinant, and the curated ARO->GO mapping projects the family-specific molecular function aminoglycoside nucleotidyltransferase activity. This focused draft accepts the candidate as a curator lead for this AMR determinant.
Supporting Evidence:
file:projects/ANTIMICROBIAL_RESISTANCE/data/candidate_new_annotations.tsv
P05058 ARO:3002623 ARO:3000218 narrower RO:0002327 GO:0034068 aminoglycoside nucleotidyltransferase activity
file:genes/BACSP/knt/knt-uniprot.txt
CC -!- FUNCTION: Inactivates aminoglycoside antibiotics such as kanamycin by CC catalyzing the transfer of a nucleotidyl group from nucleoside CC triphosphates such as (d)ATP to the 4'-hydroxyl group of the

Core Functions

knt covalently modifies aminoglycoside antibiotics by nucleotidyl transfer, reducing drug activity and contributing to antibiotic resistance.

Molecular Function:
aminoglycoside nucleotidyltransferase activity
Directly Involved In:
response to antibiotic
Supporting Evidence:
  • file:genes/BACSP/knt/knt-uniprot.txt
    CC -!- FUNCTION: Inactivates aminoglycoside antibiotics such as kanamycin by CC catalyzing the transfer of a nucleotidyl group from nucleoside CC triphosphates such as (d)ATP to the 4'-hydroxyl group of the

References

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Suggested Questions for Experts

Q: Is the ARO-derived aminoglycoside nucleotidyltransferase activity annotation sufficiently specific for knt, or is a narrower substrate/site-specific GO term warranted?

Suggested Experiments

Experiment: Biochemically assay purified knt against representative antibiotic substrates for the inferred AMR family and measure loss of drug activity or target modification.

Type: in vitro enzyme assay

📚 Additional Documentation

Notes

(knt-notes.md)

Notes: knt

Focused AMR batch review. UniProt accession: P05058. Source organism: Bacillus sp.

  • UniProt/CARD provenance: DR CARD; ARO:3002623; ANT(4')-Ia; ARO:0001004; antibiotic inactivation.
  • ARO-to-GO candidate: GO:0034068 (aminoglycoside nucleotidyltransferase activity) from projects/ANTIMICROBIAL_RESISTANCE/data/candidate_new_annotations.tsv and projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml.
  • This is a DRAFT focused review intended to cover the AMR annotation gap; it has not had a gene-specific deep-research pass.

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