DnaD is a DNA-binding and DNA-remodeling protein that helps assemble the Bacillus subtilis replication-initiation machinery. It recognizes a single-stranded DnaD recognition element at oriC and links DnaA-dependent origin assembly to DnaB and subsequent DnaI-mediated loading of DnaC helicase. DnaD also participates in replication restart and can form DNA-remodeling scaffolds without nicking DNA.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005737 cytoplasm | EXP PMID:15186423 Control of DNA replication initiation by recruitment of an e... | ACCEPT | Summary: DnaD is predominantly cytoplasmic. Membrane recruitment observed with the dnaB S371P mutant is a distinct experimental condition and is not added as a wild-type location. Reason: This location is consistent with the protein’s chromosome-associated work. DnaD is predominantly cytoplasmic. Membrane recruitment observed with the dnaB S371P mutant is a distinct experimental condition and is not added as a wild-type location. Supporting Evidence: |
| GO:0005737 cytoplasm | IEA GO_REF:0000044 | ACCEPT | Summary: DnaD is predominantly cytoplasmic. Membrane recruitment observed with the dnaB S371P mutant is a distinct experimental condition and is not added as a wild-type location. Reason: This location is consistent with the protein’s chromosome-associated work. DnaD is predominantly cytoplasmic. Membrane recruitment observed with the dnaB S371P mutant is a distinct experimental condition and is not added as a wild-type location. Supporting Evidence: |
| GO:0003688 DNA replication origin binding | IDA PMID:37093985 The DNA replication initiation protein DnaD recognises a spe... | NEW | Summary: Add the directly demonstrated DNA-engagement function. Reason: The protein itself binds the DNA substrate and organizes initiation machinery. This is direct molecular participation, not a process assertion based only on essentiality. The original seed lacks this claim. Supporting Evidence: PMID:37093985 Biochemical and genetic data support the model that DnaD recognizes a new single-stranded DNA (ssDNA) motif located in oriC, the DnaD Recognition Element (DRE). |
| GO:0006270 DNA replication initiation | IC PMID:37093985 The DNA replication initiation protein DnaD recognises a spe... | NEW | Summary: Add direct participation in origin-dependent replication initiation. Reason: The protein itself binds the DNA substrate and organizes initiation machinery. This is direct molecular participation, not a process assertion based only on essentiality. The original seed lacks this claim. Supporting Evidence: PMID:37093985 Biochemical and genetic data support the model that DnaD recognizes a new single-stranded DNA (ssDNA) motif located in oriC, the DnaD Recognition Element (DRE). |
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Download this section (compressed HTML)Q: A verified bacterial helicase-loader molecular-function term capturing this protein’s precise loading contribution was not identified. A bacterial helicase-loading term with appropriate accessory/ATPase contribution semantics would express the established mechanism without using MCM-specific terminology. DnaI ATPase/loading and the DNA-binding accessory roles of DnaD/DnaB are supported. GO:7770122 MCM loader activity names the MCM2–7 complex and is not an appropriate substitute.
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