Bos d 2, the major cattle dander allergen: a secreted lipocalin (calycin superfamily, lipocalin family) of bovine skin/sweat glands, probably functioning as a pheromone carrier that binds small hydrophobic ligands in its beta-barrel calyx. It is a member of the cross-reactive mammalian lipocalin allergen group.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005576 extracellular region | IBA GO_REF:0000033 | ACCEPT | Summary: Bos d 2 is a secreted lipocalin; extracellular localization is well supported. Reason: Secretory lipocalin acting in the extracellular space. Supporting Evidence: file:BOVIN/Bosd2/Bosd2-uniprot.txt SUBCELLULAR LOCATION: Secreted. |
| GO:0005549 odorant binding | IBA GO_REF:0000033 | ACCEPT | Summary: Bos d 2 binds small hydrophobic odorant/pheromone ligands in its lipocalin calyx; UniProt notes a probable pheromone-carrier function. Reason: Phylogenetic inference matching the documented probable pheromone-carrier role. Supporting Evidence: file:BOVIN/Bosd2/Bosd2-uniprot.txt Probable pheromone carrier. |
| GO:0005576 extracellular region | IEA GO_REF:0000044 | ACCEPT | Summary: Automated subcellular-location annotation consistent with secretion. Reason: Consistent with the secreted nature of the lipocalin. Supporting Evidence: file:BOVIN/Bosd2/Bosd2-uniprot.txt SUBCELLULAR LOCATION: Secreted. |
| GO:0036094 small molecule binding | IEA GO_REF:0000002 | MARK AS OVER ANNOTATED | Summary: Generic parent of the more specific odorant/pheromone binding. Reason: Uninformative broad parent; the specific ligand-binding activity is already captured. |
| GO:0005550 pheromone binding | IEA file:BOVIN/Bosd2/Bosd2-uniprot.txt | NEW | Summary: NEW (proposed). UniProt describes Bos d 2 as a probable pheromone carrier; the specific molecular function pheromone binding refines the odorant-binding annotation and is not in GOA. Reason: Curated "probable pheromone carrier" supports the specific pheromone-binding molecular function. Supporting Evidence: file:BOVIN/Bosd2/Bosd2-uniprot.txt Probable pheromone carrier. |
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Download this section (compressed HTML)Q: What pheromone/odorant does Bos d 2 carry, and where (skin vs sweat gland) is this functionally relevant?
Experiment: Identify native co-purifying ligands by GC-MS and test candidate volatiles against recombinant Bos d 2 by fluorescent-probe displacement.
Hypothesis: Bos d 2 binds a specific volatile pheromone characteristic of bovine skin secretions.
Type: ligand identification / biophysical binding assay
Bos d 2, major cattle dander lipocalin; probable pheromone carrier; ACCEPT odorant binding+extracellular; over-annotated generic small molecule binding; NEW pheromone binding.
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