id: Q28133
gene_symbol: Bosd2
product_type: PROTEIN
status: DRAFT
taxon:
  id: NCBITaxon:9913
  label: Bos taurus
description: >-
  Bos d 2, the major cattle dander allergen: a secreted lipocalin (calycin
  superfamily, lipocalin family) of bovine skin/sweat glands, probably functioning
  as a pheromone carrier that binds small hydrophobic ligands in its beta-barrel
  calyx. It is a member of the cross-reactive mammalian lipocalin allergen group.
existing_annotations:
- term:
    id: GO:0005576
    label: extracellular region
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: is_active_in
  review:
    summary: Bos d 2 is a secreted lipocalin; extracellular localization is well supported.
    action: ACCEPT
    reason: Secretory lipocalin acting in the extracellular space.
    supported_by:
    - reference_id: file:BOVIN/Bosd2/Bosd2-uniprot.txt
      supporting_text: 'SUBCELLULAR LOCATION: Secreted.'
- term:
    id: GO:0005549
    label: odorant binding
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: enables
  review:
    summary: Bos d 2 binds small hydrophobic odorant/pheromone ligands in its lipocalin calyx; UniProt notes a probable pheromone-carrier function.
    action: ACCEPT
    reason: Phylogenetic inference matching the documented probable pheromone-carrier role.
    supported_by:
    - reference_id: file:BOVIN/Bosd2/Bosd2-uniprot.txt
      supporting_text: Probable pheromone carrier.
- term:
    id: GO:0005576
    label: extracellular region
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  qualifier: located_in
  review:
    summary: Automated subcellular-location annotation consistent with secretion.
    action: ACCEPT
    reason: Consistent with the secreted nature of the lipocalin.
    supported_by:
    - reference_id: file:BOVIN/Bosd2/Bosd2-uniprot.txt
      supporting_text: 'SUBCELLULAR LOCATION: Secreted.'
- term:
    id: GO:0036094
    label: small molecule binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000002
  qualifier: enables
  review:
    summary: Generic parent of the more specific odorant/pheromone binding.
    action: MARK_AS_OVER_ANNOTATED
    reason: Uninformative broad parent; the specific ligand-binding activity is already captured.
- term:
    id: GO:0005550
    label: pheromone binding
  evidence_type: IEA
  original_reference_id: file:BOVIN/Bosd2/Bosd2-uniprot.txt
  qualifier: enables
  review:
    summary: >-
      NEW (proposed). UniProt describes Bos d 2 as a probable pheromone carrier; the
      specific molecular function pheromone binding refines the odorant-binding
      annotation and is not in GOA.
    action: NEW
    reason: Curated "probable pheromone carrier" supports the specific pheromone-binding molecular function.
    supported_by:
    - reference_id: file:BOVIN/Bosd2/Bosd2-uniprot.txt
      supporting_text: Probable pheromone carrier.
core_functions:
- description: Secreted lipocalin that binds small hydrophobic ligands; a probable pheromone carrier (major cattle dander allergen).
  molecular_function:
    id: GO:0005550
    label: pheromone binding
  supported_by:
  - reference_id: file:BOVIN/Bosd2/Bosd2-uniprot.txt
    supporting_text: Probable pheromone carrier.
  locations:
  - id: GO:0005576
    label: extracellular region
proposed_new_terms: []
suggested_questions:
- question: What pheromone/odorant does Bos d 2 carry, and where (skin vs sweat gland) is this functionally relevant?
  experts: []
suggested_experiments:
- hypothesis: Bos d 2 binds a specific volatile pheromone characteristic of bovine skin secretions.
  description: Identify native co-purifying ligands by GC-MS and test candidate volatiles against recombinant Bos d 2 by fluorescent-probe displacement.
  experiment_type: ligand identification / biophysical binding assay
references:
- id: GO_REF:0000002
  title: Gene Ontology annotation through association of InterPro records with GO terms
  findings: []
- id: GO_REF:0000033
  title: Annotation inferences using phylogenetic trees
  findings: []
- id: GO_REF:0000044
  title: Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
  findings: []
- id: file:BOVIN/Bosd2/Bosd2-uniprot.txt
  title: UniProt entry Q28133 (Allergen Bos d 2), Bos taurus
  findings:
  - statement: Bos d 2 is a secreted lipocalin and probable pheromone carrier; major cattle dander allergen.
    supporting_text: Probable pheromone carrier.
  reference_review:
    relevance: HIGH
    correctness: VERIFIED
    review_notes: Curated UniProt record; supports secreted pheromone-carrier lipocalin classification.