ERG8

Summary: IBA from the fungal/plant GHMP phosphomevalonate kinase node PTN001274235. The node is sound for fungal PTHR31814 members, and C. albicans ERG8 belongs to PTHR31814:SF2 and catalyzes the ATP-dependent conversion of 5-phosphomevalonate to 5-diphosphomevalonate.

Summary: Combined UniProt IEA from the yeast ERG8 ortholog, InterPro ERG8 family, RHEA:16341, and EC:2.7.4.2. These all point to the same accepted phosphomevalonate kinase reaction.

Summary: Manual similarity transfer from S. cerevisiae ERG8 (P24521). The transfer is correct because Candida ERG8 is in the same fungal GHMP PMK subfamily and retains the curated phosphomevalonate kinase reaction.

Summary: InterPro2GO transfer from the GHMP kinase N-terminal ATP-binding domain. ATP is the phosphoryl donor of the accepted PMK reaction, so nucleotide binding is correct but secondary to phosphomevalonate kinase activity.

Reason: ATP binding is a substrate-binding component of the core kinase reaction.

Summary: CGD ND root cellular-component placeholder; it records absence of direct C. albicans component evidence rather than a biological localization.

Reason: ACCEPT under the ND acceptance rule (projects/NO_DATA.md). The evidence this review cites for the cellular component is inferred (from orthologs, family membership or sequence features) or comes from other species; none of it is literature on this gene product in this species. This was judged from the review's own content during the NO_DATA re-action pass, not from a new literature search. Earlier action: REMOVE. Re-checked 2026-10-31: the paper cited in core_functions (PMID:11243736) is a comparative study of phosphomevalonate kinases, not a C. albicans localization. The gene's notes record a literature search that found no direct C. albicans ERG8 paper, so no study localizes this protein.

Summary: UniProt SubCell transfer to cytoplasm. This coarse location is consistent with the soluble cytosolic fungal mevalonate-pathway enzyme, and GO:0005829 is the better specific descendant.

Reason: Cytoplasm is directionally correct but general for a soluble ERG8-type phosphomevalonate kinase; cytosol is the supported descendant, matching the sibling yeast and S. pombe ERG8 reviews and the absence of any secretory or organelle targeting signal in the Candida protein.

Proposed replacements: cytosol

Summary: IBA to peroxisome derives from Eukaryota-level PAINT node PTN000781523, seeded only by Arabidopsis PMK. The family review judged this placement in need of pruning for fungal ERG8 members.

Reason: PTN000781523 places a single Arabidopsis-seeded peroxisomal PMK assertion above the plant-fungal split. Candida ERG8 ends in IGL rather than a canonical PTS1 motif and is expected to execute its mevalonate pathway step in the cytosol.

Summary: IBA from fungal PAINT node PTN001274236, seeded by yeast ERG8. The annotation is correct: C. albicans ERG8 catalyzes an early mevalonate-pathway step that supplies isopentenyl diphosphate for farnesyl diphosphate and ergosterol biosynthesis.

Summary: EnsemblFungi IEA transfer from the experimentally annotated S. cerevisiae ERG8 ortholog. The process is correct because phosphomevalonate kinase feeds the isopentenyl diphosphate and farnesyl diphosphate pools used for ergosterol production.

Summary: IBA from PTN001274235, the fungal/plant GHMP phosphomevalonate kinase node. ERG8 carries out step 2 of the three-step conversion from mevalonate to isopentenyl diphosphate.

Summary: UniPathway/orthology IEA to IPP biosynthesis via the mevalonate pathway. The assignment is the direct pathway context of the accepted phosphomevalonate kinase reaction.