MCR1

Summary: PTN000452207 supports a broad cytochrome-b5 reductase activity at a eukaryotic node, but the NADH-specific child is already captured by the target's Rhea/EC row.

Reason: PTN000452207 places a molecular-function assertion at a broad eukaryotic node seeded by characterized S. cerevisiae MCR1 and by S. cerevisiae PGA3, a plasma-membrane-associated coenzyme-Q6 reductase in the same PTHR19370:SF143 subfamily as AIM33. The node can support only the NAD(P)H-dependent GO:0004128 parent, while Candida MCR1 is separately mapped to EC 1.6.2.2 and the NADH-dependent Rhea reaction used for GO:0090524; the broad GO:0004128 claim is therefore true but entailed by the accepted NADH-specific child term for this target.

Summary: Root cellular component ND annotation: when it was made, no cellular component had been curated for this gene product from literature.

Reason: ACCEPT under the ND acceptance rule (projects/NO_DATA.md). The evidence this review cites for the cellular component is inferred (from orthologs, family membership or sequence features) or comes from other species; none of it is literature on this gene product in this species. This was judged from the review's own content during the NO_DATA re-action pass, not from a new literature search. Earlier action: REMOVE. The gene's notes record a literature search for direct C. albicans MCR1 work that found only expression/proteome or catalog records, none localizing the protein.

Summary: Candida MCR1 inherits mitochondrial localization from a broad eukaryotic PTN000452207 placement that excludes the descendant AIM33/PGA3 clade.

Reason: C. albicans MCR1 inherits the PTN000452207 mitochondrial assertion, an ancestral eukaryotic node seeded by S. cerevisiae MCR1, while PAINT explicitly withholds that localization from the descendant AIM33/PGA3 node. The inferred mitochondrial outer-membrane localization on the Candida UniProt record makes mitochondrion the right activity-site granularity for this IBA.

Summary: The UniProt subcellular-location transfer to the mitochondrial outer membrane matches the N-terminally anchored MCR1 family member.

Reason: The C. albicans Swiss-Prot record infers a single-pass mitochondrial outer-membrane protein, and the S. cerevisiae ortholog has an experimentally characterized outer-membrane isoform. This is the most specific localization currently available for Candida MCR1.

Summary: PTN000452208 propagates an inherited S. cerevisiae MCR1 ergosterol-biosynthesis assertion, but no Candida evidence currently makes the process a core MCR1 function.

Reason: The PAINT assertion comes from an S. cerevisiae MCR1 seed and C. albicans MCR1 is in the same MCR1-like branch, so the IBA is retained as an in-clade inherited process annotation. It is not promoted to a core Candida MCR1 function here because MCR1 has not been assayed in Candida, and the available CYP51 reconstitution evidence used the microsomal cytochrome-b5/NADH-cytochrome-b5-reductase system that corresponds to the ER CBR1 paralog branch rather than this mitochondrial MCR1 branch.

Summary: Root biological process ND annotation: when it was made, no biological process had been curated for this gene product from literature.

Reason: ACCEPT under the ND acceptance rule (projects/NO_DATA.md). The evidence this review cites for the biological process is inferred (from orthologs, family membership or sequence features) or comes from other species; none of it is literature on this gene product in this species. This was judged from the review's own content during the NO_DATA re-action pass, not from a new literature search. Earlier action: REMOVE. The gene's notes record a literature search for direct C. albicans MCR1 work that found only expression/proteome or catalog records, none identifying a process.

Summary: InterPro correctly detects a pyridine nucleotide cytochrome reductase, but its oxidoreductase parent is less informative than the Rhea-supported cytochrome-b5 reductase child.

Reason: MCR1 has the conserved CBR-like domains and its UniProt record already maps the protein to the NADH:cytochrome-b5 oxidoreductase reaction. The broad GO:0016491 parent loses the electron donor and acceptor specificity and is entailed by GO:0090524 on this protein.

Summary: The Rhea/EC mapping captures the expected NADH-dependent cytochrome-b5 reductase activity for Candida MCR1.

Reason: EC 1.6.2.2 and RHEA:46680 specify reduction of ferricytochrome b5 using NADH. This is the precise molecular-function term for MCR1's conserved cytochrome-b5 reductase domains; the separate GO:0016491 row is a true but too-general parent.