Can f 2, a minor dog allergen: a secreted lipocalin (calycin superfamily, lipocalin family) related to mouse major urinary protein / odorant-binding lipocalins. It binds small hydrophobic odorant ligands in its beta-barrel calyx and is produced in salivary/skin secretions and shed on hair. It is recognized by IgE in a subset of dog-allergic patients.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005576 extracellular region | IBA GO_REF:0000033 | ACCEPT | Summary: Can f 2 is a secreted lipocalin; extracellular localization is well supported. Reason: Secretory lipocalin acting in the extracellular space. Supporting Evidence: file:CANLF/Canf2/Canf2-uniprot.txt SUBCELLULAR LOCATION: Secreted |
| GO:0005549 odorant binding | IBA GO_REF:0000033 | ACCEPT | Summary: Can f 2 binds small hydrophobic odorant ligands in its lipocalin calyx, consistent with its odorant-binding-lipocalin relatives. Reason: Phylogenetic inference matching the odorant-binding lipocalin family. Supporting Evidence: file:CANLF/Canf2/Canf2-uniprot.txt Belongs to the calycin superfamily. Lipocalin family. |
| GO:0005549 odorant binding | IEA GO_REF:0000118 | ACCEPT | Summary: TreeGrafter (IEA) duplicate of the odorant-binding inference. Reason: Electronic inference matching the lipocalin odorant-binding function. Supporting Evidence: file:CANLF/Canf2/Canf2-uniprot.txt Belongs to the calycin superfamily. Lipocalin family. |
| GO:0005576 extracellular region | IEA GO_REF:0000044 | ACCEPT | Summary: Automated subcellular-location annotation consistent with secretion. Reason: Consistent with the secreted nature of the lipocalin. Supporting Evidence: file:CANLF/Canf2/Canf2-uniprot.txt SUBCELLULAR LOCATION: Secreted |
| GO:0036094 small molecule binding | IEA GO_REF:0000002 | MARK AS OVER ANNOTATED | Summary: Generic parent of the more specific odorant binding already annotated. Reason: Uninformative broad parent; the specific odorant-binding activity is already captured. |
Loading supporting content…
Download this section (compressed HTML)Q: What specific odorant/pheromone ligand does Can f 2 carry, and in which secretion is it functionally relevant?
Experiment: Characterize ligand binding of recombinant Can f 2 by fluorescent-probe displacement against a panel of odorants/lipids and identify native co-purifying ligands by GC-MS.
Hypothesis: Can f 2 binds specific volatile odorant ligands characteristic of its lipocalin subfamily.
Type: biophysical binding assay
ALLERGENS backlog (alias Canf2). Secreted odorant-binding lipocalin. IEDB: 9 epitopes.
Curation: ACCEPT odorant binding (IBA/IEA) + extracellular region; MARK_AS_OVER_ANNOTATED
generic small molecule binding. Core: odorant binding lipocalin (characterized -> low gap).
Loading supporting content…
Download this section (compressed HTML)