Can f 6, a secreted dog lipocalin (calycin superfamily, lipocalin family) and a cross-reactive mammalian allergen. It binds small hydrophobic odorant ligands in its beta-barrel calyx and is found in dog saliva and dander. Can f 6 belongs to the cross-reactive lipocalin group that includes cat Fel d 4 and horse Equ c 1, underlying IgE co-sensitization across furry-animal danders.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005576 extracellular region | IBA GO_REF:0000033 | ACCEPT | Summary: Can f 6 is a secreted lipocalin; extracellular localization is well supported. Reason: Secretory lipocalin acting in the extracellular space. Supporting Evidence: file:CANLF/Canf6/Canf6-uniprot.txt SUBCELLULAR LOCATION: Secreted |
| GO:0005549 odorant binding | IBA GO_REF:0000033 | ACCEPT | Summary: Can f 6 binds small hydrophobic odorant ligands in its lipocalin calyx. Reason: Phylogenetic inference matching the odorant-binding lipocalin family. Supporting Evidence: file:CANLF/Canf6/Canf6-uniprot.txt Belongs to the calycin superfamily. Lipocalin family. |
| GO:0005549 odorant binding | IEA GO_REF:0000118 | ACCEPT | Summary: TreeGrafter (IEA) duplicate of the odorant-binding inference. Reason: Electronic inference matching the lipocalin odorant-binding function. Supporting Evidence: file:CANLF/Canf6/Canf6-uniprot.txt Belongs to the calycin superfamily. Lipocalin family. |
| GO:0005576 extracellular region | IEA GO_REF:0000044 | ACCEPT | Summary: Automated subcellular-location annotation consistent with secretion. Reason: Consistent with the secreted nature of the lipocalin. Supporting Evidence: file:CANLF/Canf6/Canf6-uniprot.txt SUBCELLULAR LOCATION: Secreted |
| GO:0036094 small molecule binding | IEA GO_REF:0000002 | MARK AS OVER ANNOTATED | Summary: Generic parent of the more specific odorant binding already annotated. Reason: Uninformative broad parent; the specific odorant-binding activity is already captured. |
| GO:0005576 extracellular region | IDA PMID:22515174 Characterization of the dog lipocalin allergen Can f 6: the ... | ACCEPT | Summary: Can f 6 was experimentally characterized as a secreted dog lipocalin; direct assay supports its extracellular (secreted) localization. Reason: Experimental (IDA) support for the secreted/extracellular localization. |
| GO:0005576 extracellular region | IDA PMID:23464525 Dog saliva - an important source of dog allergens. | ACCEPT | Summary: Can f 6 was identified in dog saliva, an experimental source of secreted dog allergens. Reason: Experimental (IDA) detection in dog saliva supports extracellular localization. Supporting Evidence: PMID:23464525 while Can f 2 and Can f 6 |
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Download this section (compressed HTML)Q: What endogenous odorant/lipid does Can f 6 carry, and does ligand identity explain its cross-reactivity with Fel d 4 and Equ c 1?
Experiment: Compare ligand binding of recombinant Can f 6, Fel d 4 and Equ c 1 by fluorescent-probe displacement and map shared IgE epitopes by cross-inhibition.
Hypothesis: Can f 6 shares ligand-binding specificity with the cross-reactive lipocalins Fel d 4 and Equ c 1.
Type: biophysical / cross-reactivity assay
ALLERGENS backlog (alias Canf6). Secreted odorant-binding lipocalin; cross-reactive
with cat Fel d 4 and horse Equ c 1 PMID:22515174. Found in dog saliva PMID:23464525. IEDB: 5 epitopes, IgE+.
Curation: ACCEPT odorant binding + extracellular region (incl. IDA from saliva proteomics);
MARK_AS_OVER_ANNOTATED generic small molecule binding. Core: odorant binding lipocalin.
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