id: H2B3G5
gene_symbol: Canf6
product_type: PROTEIN
status: DRAFT
taxon:
  id: NCBITaxon:9615
  label: Canis lupus familiaris
description: >-
  Can f 6, a secreted dog lipocalin (calycin superfamily, lipocalin family) and a
  cross-reactive mammalian allergen. It binds small hydrophobic odorant ligands in
  its beta-barrel calyx and is found in dog saliva and dander. Can f 6 belongs to
  the cross-reactive lipocalin group that includes cat Fel d 4 and horse Equ c 1,
  underlying IgE co-sensitization across furry-animal danders.
existing_annotations:
- term:
    id: GO:0005576
    label: extracellular region
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: is_active_in
  review:
    summary: Can f 6 is a secreted lipocalin; extracellular localization is well supported.
    action: ACCEPT
    reason: Secretory lipocalin acting in the extracellular space.
    supported_by:
    - reference_id: file:CANLF/Canf6/Canf6-uniprot.txt
      supporting_text: 'SUBCELLULAR LOCATION: Secreted'
- term:
    id: GO:0005549
    label: odorant binding
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  qualifier: enables
  review:
    summary: Can f 6 binds small hydrophobic odorant ligands in its lipocalin calyx.
    action: ACCEPT
    reason: Phylogenetic inference matching the odorant-binding lipocalin family.
    supported_by:
    - reference_id: file:CANLF/Canf6/Canf6-uniprot.txt
      supporting_text: Belongs to the calycin superfamily. Lipocalin family.
- term:
    id: GO:0005549
    label: odorant binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000118
  qualifier: enables
  review:
    summary: TreeGrafter (IEA) duplicate of the odorant-binding inference.
    action: ACCEPT
    reason: Electronic inference matching the lipocalin odorant-binding function.
    supported_by:
    - reference_id: file:CANLF/Canf6/Canf6-uniprot.txt
      supporting_text: Belongs to the calycin superfamily. Lipocalin family.
- term:
    id: GO:0005576
    label: extracellular region
  evidence_type: IEA
  original_reference_id: GO_REF:0000044
  qualifier: located_in
  review:
    summary: Automated subcellular-location annotation consistent with secretion.
    action: ACCEPT
    reason: Consistent with the secreted nature of the lipocalin.
    supported_by:
    - reference_id: file:CANLF/Canf6/Canf6-uniprot.txt
      supporting_text: 'SUBCELLULAR LOCATION: Secreted'
- term:
    id: GO:0036094
    label: small molecule binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000002
  qualifier: enables
  review:
    summary: Generic parent of the more specific odorant binding already annotated.
    action: MARK_AS_OVER_ANNOTATED
    reason: Uninformative broad parent; the specific odorant-binding activity is already captured.
- term:
    id: GO:0005576
    label: extracellular region
  evidence_type: IDA
  original_reference_id: PMID:22515174
  qualifier: located_in
  review:
    summary: >-
      Can f 6 was experimentally characterized as a secreted dog lipocalin; direct
      assay supports its extracellular (secreted) localization.
    action: ACCEPT
    reason: Experimental (IDA) support for the secreted/extracellular localization.
- term:
    id: GO:0005576
    label: extracellular region
  evidence_type: IDA
  original_reference_id: PMID:23464525
  qualifier: located_in
  review:
    summary: Can f 6 was identified in dog saliva, an experimental source of secreted dog allergens.
    action: ACCEPT
    reason: Experimental (IDA) detection in dog saliva supports extracellular localization.
    supported_by:
    - reference_id: PMID:23464525
      supporting_text: while Can f 2 and Can f 6
core_functions:
- description: >-
    Secreted odorant-binding lipocalin that binds small hydrophobic odorant ligands
    in its beta-barrel calyx; member of the cross-reactive mammalian lipocalin group
    (with Fel d 4 and Equ c 1).
  molecular_function:
    id: GO:0005549
    label: odorant binding
  supported_by:
  - reference_id: file:CANLF/Canf6/Canf6-uniprot.txt
    supporting_text: Belongs to the calycin superfamily. Lipocalin family.
  locations:
  - id: GO:0005576
    label: extracellular region
proposed_new_terms: []
suggested_questions:
- question: What endogenous odorant/lipid does Can f 6 carry, and does ligand identity explain its cross-reactivity with Fel d 4 and Equ c 1?
  experts: []
suggested_experiments:
- hypothesis: Can f 6 shares ligand-binding specificity with the cross-reactive lipocalins Fel d 4 and Equ c 1.
  description: >-
    Compare ligand binding of recombinant Can f 6, Fel d 4 and Equ c 1 by
    fluorescent-probe displacement and map shared IgE epitopes by cross-inhibition.
  experiment_type: biophysical / cross-reactivity assay
references:
- id: GO_REF:0000002
  title: Gene Ontology annotation through association of InterPro records with GO terms
  findings: []
- id: GO_REF:0000033
  title: Annotation inferences using phylogenetic trees
  findings: []
- id: GO_REF:0000044
  title: Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
  findings: []
- id: GO_REF:0000118
  title: TreeGrafter-generated GO annotations
  findings: []
- id: PMID:22515174
  title: 'Characterization of the dog lipocalin allergen Can f 6: the role in cross-reactivity with cat and horse.'
  findings:
  - statement: Can f 6 is a dog lipocalin allergen cross-reactive with cat (Fel d 4) and horse (Equ c 1) lipocalins.
  reference_review:
    relevance: HIGH
    correctness: VERIFIED
    review_notes: Primary characterization of Can f 6 as a cross-reactive lipocalin allergen; supports secreted localization and the cross-reactive-lipocalin grouping.
- id: PMID:23464525
  title: Dog saliva - an important source of dog allergens.
  findings:
  - statement: Can f 6 is present in dog saliva, a major source of secreted dog allergens.
  reference_review:
    relevance: MEDIUM
    correctness: VERIFIED
    review_notes: Supports the secreted/salivary (extracellular) localization of Can f 6.