# Pair analysis: slc7a10a / slc7a10b  (run 2026-09-28)

## 1. Ensembl Compara: paralogue call and orthologues

slc7a10a / slc7a10b: Compara type within_species_paralog, duplication node Osteoglossocephalai, %id 76.7123 / 75.2399

| species | Ensembl gene | protein | length | orthology to slc7a10a | slc7a10b |
|---|---|---|---|---|---|
| lepisosteus_oculatus | ENSLOCG00000001884 | ENSLOCP00000002188 | 515 | ortholog_one2many | ortholog_one2many |
| latimeria_chalumnae | ENSLACG00000010247 | ENSLACP00000011644 | 345 | ortholog_one2many | ortholog_one2many |
| xenopus_tropicalis | ENSXETG00000018279 | ENSXETP00000039618 | 530 | ortholog_one2many | ortholog_one2many |
| gallus_gallus | ENSGALG00010025010 | ENSGALP00010037783 | 525 | ortholog_one2many | ortholog_one2many |
| homo_sapiens | ENSG00000130876 | ENSP00000253188 | 523 | ortholog_one2many | ortholog_one2many |
| mus_musculus | ENSMUSG00000030495 | ENSMUSP00000001854 | 530 | ortholog_one2many | ortholog_one2many |
| scleropages_formosus | ENSSFOG00015011468 | ENSSFOP00015060938 | 508 | ortholog_one2one | - |
| clupea_harengus | ENSCHAG00020014087 | ENSCHAP00020031625 | 527 | ortholog_one2one | - |
| clupea_harengus | ENSCHAG00020007437 | ENSCHAP00020015871 | 525 | - | ortholog_one2one |
| astyanax_mexicanus | ENSAMXG00000020227 | ENSAMXP00000020828 | 505 | ortholog_one2one | - |
| astyanax_mexicanus | ENSAMXG00000013943 | ENSAMXP00000014342 | 527 | - | ortholog_one2one |
| esox_lucius | ENSELUG00000018150 | ENSELUP00000049282 | 516 | ortholog_one2one | - |
| esox_lucius | ENSELUG00000001270 | ENSELUP00000085085 | 528 | - | ortholog_one2one |
| salmo_salar | ENSSSAG00000116744 | ENSSSAP00000182186 | 523 | ortholog_one2many | - |
| salmo_salar | ENSSSAG00000094872 | ENSSSAP00000083221 | 523 | ortholog_one2many | - |
| salmo_salar | ENSSSAG00000090580 | ENSSSAP00000083134 | 528 | - | ortholog_one2many |
| salmo_salar | ENSSSAG00000100300 | ENSSSAP00000083040 | 528 | - | ortholog_one2many |
| gadus_morhua | ENSGMOG00000026425 | ENSGMOP00000068501 | 571 | ortholog_one2one | - |
| gadus_morhua | ENSGMOG00000011136 | ENSGMOP00000032400 | 512 | - | ortholog_one2one |
| oryzias_latipes | ENSORLG00000015054 | ENSORLP00000018870 | 509 | ortholog_one2one | - |
| oryzias_latipes | ENSORLG00000018320 | ENSORLP00000022941 | 518 | - | ortholog_one2one |
| gasterosteus_aculeatus | ENSGACG00000014873 | ENSGACP00000019645 | 500 | ortholog_one2one | - |
| gasterosteus_aculeatus | ENSGACG00000008186 | ENSGACP00000052288 | 510 | - | ortholog_one2one |
| takifugu_rubripes | ENSTRUG00000009024 | ENSTRUP00000061890 | 497 | ortholog_one2one | - |
| takifugu_rubripes | ENSTRUG00000033356 | ENSTRUP00000067903 | 263 | - | ortholog_one2one |
| oreochromis_niloticus | ENSONIG00000003132 | ENSONIP00000031609 | 549 | ortholog_one2one | - |
| oreochromis_niloticus | ENSONIG00000010619 | ENSONIP00000064405 | 519 | - | ortholog_one2one |

## 2. Pairwise identities

length slc7a10a: 511 aa
length slc7a10b: 517 aa
length human SLC7A10: 523 aa
length human SLC7A5: 507 aa
length human SLC7A8: 535 aa
identity slc7a10a vs slc7a10b: 77.8% over 523 columns
identity slc7a10a vs human SLC7A10: 69.0% over 525 columns
identity slc7a10a vs human SLC7A5: 47.3% over 522 columns
identity slc7a10a vs human SLC7A8: 61.7% over 538 columns
identity slc7a10b vs human SLC7A10: 70.2% over 523 columns
identity slc7a10b vs human SLC7A5: 45.8% over 530 columns
identity slc7a10b vs human SLC7A8: 60.3% over 544 columns
identity slc7a10a vs gar ENSLOCG00000001884 (515 aa): 78.6% over 515 columns
identity slc7a10b vs gar ENSLOCG00000001884 (515 aa): 80.2% over 524 columns
identity human SLC7A10 vs gar ENSLOCG00000001884: 72.0% over 533 columns

## 3. Conservation of annotated sites of the human reference

| human position | annotation | slc7a10a | slc7a10b | latimeria:ENSLACG00000010247 | lepisosteus:ENSLOCG00000001884 | oryzias:ENSORLG00000015054 | oryzias:ENSORLG00000018320 |
|---|---|---|---|---|---|---|---|
| N52 | site tested in literature (see config) | N | N | N | N | N | N |
| Y131 | site tested in literature (see config) | Y | Y | Y | Y | Y | Y |
| I138 | site tested in literature (see config) | I | I | I | I | I | I |
| C154 | site tested in literature (see config) | C | C | C | C | C | C |
| F243 | site tested in literature (see config) | F | F | F | F | F | F |
| F250 | site tested in literature (see config) | F | F | F | F | F | F |
| Y253 | site tested in literature (see config) | Y | Y | Y | Y | Y | Y |
| E257 | site tested in literature (see config) | E | E | E | E | E | E |
| Y333 | site tested in literature (see config) | Y | Y | Y | Y | Y | Y |
| R339 | site tested in literature (see config) | R | R | R | R | R | R |

## 6. Tajima-style relative-rate test (outgroup = gar)

gar ENSLOCG00000001884 positions aligned in both copies: 508
changes unique to slc7a10a (m1): 42; unique to slc7a10b (m2): 25; chi2 (1 df) = 4.31 (P<0.05)

## 7. Whole-embryo time course, E-ERAD-475 (TPM, median of replicates)

| stage | slc7a10a | slc7a10b |
|---|---|---|
| zygote | 0 | 0 |
| cleavage 2-cell | 0 | 0 |
| blastula 128-cell | 0.3 | 1 |
| blastula 1k-cell | 0 | 0 |
| blastula dome | 0 | 2 |
| gastrula 50%-epiboly | 0 | 3 |
| gastrula shield | 0 | 2 |
| gastrula 75%-epiboly | 0 | 4 |
| segmentation 1-4 somites | 0.7 | 6 |
| segmentation 14-19 somites | 1 | 6 |
| segmentation 20-25 somites | 1 | 4 |
| pharyngula prim-5 | 2 | 3 |
| pharyngula prim-15 | 2 | 3 |
| pharyngula prim-25 | 2 | 4 |
| hatching long-pec | 5 | 2 |
| larval protruding mouth | 29 | 6 |
| larval day 4 | 26 | 9 |
| larval day 5 | 19 | 9 |

## 8. Bgee expressed calls per anatomical entity (score 0-100; only 'expressed' calls are returned)

### slc7a10a: 21 calls
- bone element: 89.8 (gold; RNA-Seq)
- brain: 86.9 (gold; RNA-Seq)
- heart: 83.8 (gold; RNA-Seq)
- mesonephros: 82.1 (gold; RNA-Seq)
- larva: 80.4 (gold; RNA-Seq)
- head kidney: 78.6 (gold; RNA-Seq)
- muscle tissue: 71.8 (gold; RNA-Seq)
- testis: 69.6 (gold; RNA-Seq)
- zone of skin: 68.5 (gold; RNA-Seq)
- swim bladder: 67.3 (gold; RNA-Seq)
- granulocyte: 66.5 (gold; RNA-Seq)
- intestine: 64.7 (gold; RNA-Seq)
- head: 62.6 (gold; RNA-Seq)
- pharyngeal gill: 61.5 (gold; RNA-Seq)
- spleen: 58.6 (gold; RNA-Seq)
- tail: 52.1 (gold; RNA-Seq)
- camera-type eye: 51.3 (silver; RNA-Seq)
- liver: 48.5 (gold; RNA-Seq)
- embryo: 39.8 (gold; RNA-Seq)
- ovary: 34.9 (silver; RNA-Seq)
- blastula: 28.5 (silver; RNA-Seq)
### slc7a10b: 8 calls
- brain: 73.6 (gold; RNA-Seq)
- testis: 54.6 (gold; RNA-Seq)
- gastrula: 52.7 (gold; RNA-Seq)
- blastula: 45.5 (silver; RNA-Seq)
- head: 45.1 (gold; RNA-Seq)
- larva: 44.8 (gold; RNA-Seq)
- camera-type eye: 36.8 (silver; RNA-Seq)
- bone element: 36.6 (gold; RNA-Seq)
### gar ENSLOCG00000001884: 8 calls
- brain: 63.7 (gold; RNA-Seq)
- camera-type eye: 58.7 (gold; RNA-Seq)
- bone element: 44.8 (gold; RNA-Seq)
- ovary: 43.1 (gold; RNA-Seq)
- larva: 42.3 (gold; RNA-Seq)
- embryo: 28.9 (gold; RNA-Seq)
- testis: 26.4 (silver; RNA-Seq)
- zone of skin: 18.3 (silver; RNA-Seq)

RNA-Seq-supported entities, slc7a10a only: ['embryo', 'granulocyte', 'head kidney', 'heart', 'intestine', 'liver', 'mesonephros', 'muscle tissue', 'ovary', 'pharyngeal gill', 'spleen', 'swim bladder', 'tail', 'zone of skin']
RNA-Seq-supported entities, slc7a10b only: ['gastrula']
RNA-Seq-supported entities, both: ['blastula', 'bone element', 'brain', 'camera-type eye', 'head', 'larva', 'testis']

Score comparison in shared RNA-Seq entities (score difference >= 10 flagged):
- blastula: slc7a10a 28.5 vs slc7a10b 45.5 <--
- bone element: slc7a10a 89.8 vs slc7a10b 36.6 <--
- brain: slc7a10a 86.9 vs slc7a10b 73.6 <--
- camera-type eye: slc7a10a 51.3 vs slc7a10b 36.8 <--
- head: slc7a10a 62.6 vs slc7a10b 45.1 <--
- larva: slc7a10a 80.4 vs slc7a10b 44.8 <--
- testis: slc7a10a 69.6 vs slc7a10b 54.6 <--

## 9. ZFIN curated wild-type expression

### slc7a10a: 0 records
### slc7a10b: 0 records

## 10. Local synteny between the two copies (Ensembl REST)

slc7a10a: chr7:38315647-38340674
slc7a10b: chr25:36266782-36286186

slc7a10a +/- 1.5 Mb: 66 other protein-coding genes (0 homology queries failed); 13 have a teleost-level (Teleostei/Osteoglossocephalai/Clupeocephala) zebrafish paralogue; 1 of those paralogues lie within 1.5 Mb of slc7a10b:
- sall1a -> sall1b (chr25:35986345)

slc7a10b +/- 1.5 Mb: 153 other protein-coding genes (0 homology queries failed); 51 have a teleost-level (Teleostei/Osteoglossocephalai/Clupeocephala) zebrafish paralogue; 1 of those paralogues lie within 1.5 Mb of slc7a10a:
- sall1b -> sall1a (chr7:37359004)