Q9RSY6

UniProt ID: Q9RSY6
Organism: Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1)
Review Status: DRAFT
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Gene Description

Small ribosomal subunit protein bS1 (30S ribosomal protein S1) of Deinococcus radiodurans R1 is a 629-amino-acid RNA-binding protein containing five S1/OB-fold domains. It is the largest protein of the bacterial 30S ribosomal subunit and plays an essential role in translation initiation by recruiting mRNAs to the ribosome through interactions with the 5-prime untranslated region. The protein belongs to the bacterial ribosomal protein bS1 family (COG0539) and is encoded by DR_1983 on Chromosome 1. The N-terminal region is disordered and enriched in polar residues, while the five tandem S1 motifs (residues 122-539) mediate RNA binding and ribosome association.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0003729 mRNA binding
IBA
GO_REF:0000033 		ACCEPT
Summary: Ribosomal protein S1 is well established as the primary mRNA-recruiting factor on the bacterial 30S ribosomal subunit. Its multiple S1/OB-fold domains bind the 5-prime UTR of mRNAs to position them for translation initiation. The IBA annotation is inferred from experimentally characterized orthologs including E. coli RpsA (P0AG67), where mRNA binding by S1 has been directly demonstrated. This is a core molecular function of bS1.
Reason: mRNA binding is the primary molecular activity of ribosomal protein S1, supported by extensive experimental evidence in E. coli orthologs and consistent with the five S1/OB-fold RNA-binding domains in this protein.
Supporting Evidence:
file:DEIRA/Q9RSY6/Q9RSY6-uniprot.txt
RNA-binding {ECO:0000256|ARBA:ARBA00022884}
file:DEIRA/Q9RSY6/Q9RSY6-uniprot.txt
RecName: Full=Small ribosomal subunit protein bS1
GO:0003735 structural constituent of ribosome
IBA
GO_REF:0000033 		ACCEPT
Summary: S1 is a component of the bacterial 30S ribosomal subunit and contributes to ribosome structure, although it is more loosely associated than most ribosomal proteins. The IBA annotation is inferred from E. coli RpsA (P0AG67) via PANTHER phylogenetic trees. The UniProt keywords Ribonucleoprotein and Ribosomal protein further support this assignment.
Reason: S1 is an integral component of the 30S ribosomal subunit that contributes to its structural integrity and function. The ribosomal protein family assignment and domain architecture confirm this role.
Supporting Evidence:
file:DEIRA/Q9RSY6/Q9RSY6-uniprot.txt
Ribosomal protein {ECO:0000256|ARBA:ARBA00022980, ECO:0000313|EMBL:AAF11535.1}
file:DEIRA/Q9RSY6/Q9RSY6-uniprot.txt
Ribonucleoprotein {ECO:0000256|ARBA:ARBA00023274}
file:DEIRA/Q9RSY6/Q9RSY6-uniprot.txt
SIMILARITY: Belongs to the bacterial ribosomal protein bS1 family.
GO:0006412 translation
IBA
GO_REF:0000033 		ACCEPT
Summary: As a component of the 30S ribosomal subunit, bS1 is directly involved in translation. In bacteria, S1 plays an especially important role in translation initiation by recruiting mRNAs to the ribosome. The IBA annotation is well supported by phylogenetic inference from experimentally characterized orthologs including E. coli RpsA.
Reason: Translation is the fundamental biological process in which ribosomal protein S1 participates, both as a structural component of the ribosome and through its specialized role in mRNA recruitment during initiation.
Supporting Evidence:
file:DEIRA/Q9RSY6/Q9RSY6-uniprot.txt
Ribosomal protein {ECO:0000256|ARBA:ARBA00022980, ECO:0000313|EMBL:AAF11535.1}
file:DEIRA/Q9RSY6/Q9RSY6-uniprot.txt
RecName: Full=Small ribosomal subunit protein bS1
GO:0022627 cytosolic small ribosomal subunit
IBA
GO_REF:0000033 		ACCEPT
Summary: S1 is a component of the bacterial 30S ribosomal subunit, which is the cytosolic small ribosomal subunit in bacteria. The IBA annotation is inferred from E. coli RpsA (P0AG67) via PANTHER phylogenetic trees. D. radiodurans ribosomes are cytoplasmic, and the 30S subunit corresponds to the cytosolic small ribosomal subunit.
Reason: Bacterial ribosomal protein S1 is a component of the 30S (cytosolic small) ribosomal subunit. This cellular component annotation accurately reflects the localization of bS1 within the ribosome.
Supporting Evidence:
file:DEIRA/Q9RSY6/Q9RSY6-uniprot.txt
RecName: Full=Small ribosomal subunit protein bS1
file:DEIRA/Q9RSY6/Q9RSY6-uniprot.txt
AltName: Full=30S ribosomal protein S1
GO:0003676 nucleic acid binding
IEA
GO_REF:0000002 		MARK AS OVER ANNOTATED
Summary: This generic term is mapped from InterPro domain IPR003029 (S1 domain) via InterPro2GO. While S1 does bind nucleic acids, the more specific child term GO:0003729 (mRNA binding) already captures the biologically relevant RNA-binding activity. The generic nucleic acid binding term adds no information beyond what the specific mRNA binding annotation provides.
Reason: GO:0003729 (mRNA binding) already annotates this protein at a more informative level. The generic parent term nucleic acid binding is redundant and represents an over-annotation from automatic InterPro2GO mapping.
Supporting Evidence:
file:DEIRA/Q9RSY6/Q9RSY6-uniprot.txt
InterPro; IPR003029; S1_domain.
file:DEIRA/Q9RSY6/Q9RSY6-uniprot.txt
RNA-binding {ECO:0000256|ARBA:ARBA00022884}
GO:0003729 mRNA binding
IEA
GO_REF:0000117 		ACCEPT
Summary: This ARBA-generated IEA annotation for mRNA binding is correct and consistent with the IBA annotation for the same term. It provides independent computational support for the mRNA binding function of bS1.
Reason: Correct annotation consistent with the IBA evidence for the same GO term (GO:0003729). mRNA binding is a core molecular function of bS1.
Supporting Evidence:
file:DEIRA/Q9RSY6/Q9RSY6-uniprot.txt
RNA-binding {ECO:0000256|ARBA:ARBA00022884}
GO:0005737 cytoplasm
IEA
GO_REF:0000117 		KEEP AS NON CORE
Summary: This ARBA-generated annotation placing bS1 in the cytoplasm is correct since bacterial ribosomes reside in the cytoplasm. However, the more specific cellular component annotation GO:0022627 (cytosolic small ribosomal subunit) already captures the localization of this protein within the ribosome. The cytoplasm annotation is less informative but not incorrect.
Reason: Correct but less specific than GO:0022627 (cytosolic small ribosomal subunit) which already precisely places S1 in its functional context. Retained as a broad localization annotation.
Supporting Evidence:
file:DEIRA/Q9RSY6/Q9RSY6-uniprot.txt
RecName: Full=Small ribosomal subunit protein bS1

Core Functions

bS1 functions as an mRNA-recruiting component of the 30S ribosomal subunit in D. radiodurans, binding mRNA 5-prime UTRs through its five S1/OB-fold domains to facilitate translation initiation.

Molecular Function:
mRNA binding
Directly Involved In:
translation
In Complex:
cytosolic small ribosomal subunit
Supporting Evidence:
  • file:DEIRA/Q9RSY6/Q9RSY6-uniprot.txt
    RecName: Full=Small ribosomal subunit protein bS1
  • file:DEIRA/Q9RSY6/Q9RSY6-uniprot.txt
    RNA-binding {ECO:0000256|ARBA:ARBA00022884}
  • file:DEIRA/Q9RSY6/Q9RSY6-uniprot.txt
    SIMILARITY: Belongs to the bacterial ribosomal protein bS1 family.

References

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External Prediction Reviews

These computational predictions are reviewed separately from the GOA annotation set used for this review. The assessments below are from this project and do not constitute official GO annotations or endorsement by GO/UniProt. They are not included in the existing annotation review above.

ProtNLM2 External predictions

View prediction review YAML · Q9RSY6-protnlm-predictions-review.yaml · Review status: COMPLETE

The bS1 ribosomal-protein architecture supports both predictions, which are less specific than the existing curated RNA-binding and ribosomal-subunit annotations.

Source documents: genes/DEIRA/Q9RSY6/Q9RSY6-uniprot.txt · genes/DEIRA/Q9RSY6/Q9RSY6-goa.tsv · publications/PMID_9451450.md

Review score: 2 = concordant with evidence; 1 = uncertain; 0 = discordant with evidence. This is an assessment score, not a model probability.

GO:0003676 nucleic acid binding GO_MF
LSP — Less precise than existing annotation Review score: 2/2
Prediction method: ProtNLM2 · Version: UniProt 2024_06 pilot
Review rationale: The sequence contains multiple S1 RNA-binding domains and is assigned to the bacterial bS1 family. Experimental work on E. coli S1 demonstrates RNA binding (PMID:9451450), consistent with the target curated IBA for mRNA binding. Nucleic acid binding is a broader molecular function than mRNA binding. The prediction is supported but loses the established substrate specificity.
Supporting Evidence:
  • file:DEIRA/Q9RSY6/Q9RSY6-uniprot.txt: "ID Q9RSY6_DEIRA Unreviewed; 629 AA. ... DR GO; GO:0022627; C:cytosolic small ribosomal subunit; IBA:GO_Central. ... DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central. ... DR GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central. ... DR GO; GO:0006412; P:translation; IBA:GO_Central. ... DR InterPro; IPR050437; Ribos_protein_bS1-like. ... DR InterPro; IPR003029; S1_domain. ... DR PANTHER; PTHR10724; 30S RIBOSOMAL PROTEIN S1; 1. ... DR PANTHER; PTHR10724:SF7; SMALL RIBOSOMAL SUBUNIT PROTEIN BS1C; 1. ... FT DOMAIN 122..193 ... FT /note="S1 motif" ... FT DOMAIN 211..277 ... FT /note="S1 motif" ... FT DOMAIN 298..366 ... FT /note="S1 motif" ... FT DOMAIN 383..453 ... FT /note="S1 motif" ... FT DOMAIN 470..539 ... FT /note="S1 motif""
  • PMID:9451450: "The binding of S1 to poly(A) occurred"
GO:0005840 ribosome GO_CC
LSP — Less precise than existing annotation Review score: 2/2
Prediction method: ProtNLM2 · Version: UniProt 2024_06 pilot
Review rationale: The target bS1 family assignment and repeated S1-domain architecture support its role in the bacterial small ribosomal subunit. This placement is also represented by the curated IBA for cytosolic small ribosomal subunit, with the established RNA-binding behavior of S1 providing functional context (PMID:9451450). Ribosome is a broader compartment than the annotated subunit. The prediction is supported but less precise.
Supporting Evidence:
  • file:DEIRA/Q9RSY6/Q9RSY6-uniprot.txt: "ID Q9RSY6_DEIRA Unreviewed; 629 AA. ... DR GO; GO:0022627; C:cytosolic small ribosomal subunit; IBA:GO_Central. ... DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central. ... DR GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central. ... DR GO; GO:0006412; P:translation; IBA:GO_Central. ... DR InterPro; IPR050437; Ribos_protein_bS1-like. ... DR InterPro; IPR003029; S1_domain. ... DR PANTHER; PTHR10724; 30S RIBOSOMAL PROTEIN S1; 1. ... DR PANTHER; PTHR10724:SF7; SMALL RIBOSOMAL SUBUNIT PROTEIN BS1C; 1. ... FT DOMAIN 122..193 ... FT /note="S1 motif" ... FT DOMAIN 211..277 ... FT /note="S1 motif" ... FT DOMAIN 298..366 ... FT /note="S1 motif" ... FT DOMAIN 383..453 ... FT /note="S1 motif" ... FT DOMAIN 470..539 ... FT /note="S1 motif""
  • PMID:9451450: "The binding of S1 to poly(A) occurred"

Deep Research

Falcon

(Q9RSY6-deep-research-falcon.md)

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