CG10359

Summary: A specific GPCR interaction is not established by the fibrinogen-like domain.

Reason: The ISS assertion is a curated transfer rather than an experiment on this protein. Its fibrinogen-like domain and signal peptide support extracellular biology but do not identify a GPCR partner or establish conserved receptor binding.

Summary: Participation in GPCR signaling requires a specific ligand-receptor relationship.

Reason: The sequence supports a secretory fibrinogen-domain protein but does not establish the receptor or pathway represented by this ISS annotation.

Summary: Chitin binding is not a universal fibrinogen-domain property.

Reason: The extracellular fold permits diverse ligands; target-specific binding or a resolved transfer from a characterized chitin-binding ortholog is needed.

Summary: Secretion does not by itself establish membrane association.

Reason: The source record contains a signal peptide at residues 1-23 but no retained transmembrane segment. Peripheral membrane binding remains possible; the ISS membrane assertion cannot be resolved from this architecture alone.

Summary: Extracellular-matrix residence is more specific than secretory-pathway entry.

Reason: The IBA is a curated phylogenetic assertion, but a fibrinogen-like domain and signal peptide alone do not establish matrix residence. PMID:24675716 describes a candidate angiopoietin-like protein without establishing ligand or matrix binding. The relevant ancestral localization inference remains unresolved.