CG31099

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0106389 ecdysteroid 22-kinase activity	TAS PMID:32540344 Genomic and transcriptomic analyses in Drosophila suggest th...	UNDECIDED	Summary: The cited study proposes family-wide detoxification-by-phosphorylation hypotheses from genomic and expression analyses. Its accessible abstract does not establish CG31099-catalyzed phosphorylation of an ecdysteroid specifically at position 22. The kinase-like family assignment supports a candidate catalytic role, but exact substrate and positional specificity require target-specific or well-resolved subfamily evidence. Reason: The cited study proposes family-wide detoxification-by-phosphorylation hypotheses from genomic and expression analyses. Its accessible abstract does not establish CG31099-catalyzed phosphorylation of an ecdysteroid specifically at position 22. The kinase-like family assignment supports a candidate catalytic role, but exact substrate and positional specificity require target-specific or well-resolved subfamily evidence. Supporting Evidence: PMID:32540344 We propose the hypothesis that members of the arthropod-specific ecdysteroid kinase-like (EcKL) gene family encode detoxicative kinases. file:DROME/CG31099/CG31099-uniprot.txt /note="CHK kinase-like" PMID:38291829 Our results show the only two genes known to encode ecdysteroid kinases belong to different subfamilies and therefore ecdysteroid metabolism functions must be spread throughout the EcKL family.

GO Term

Evidence

Action

Reason

GO:0106389 ecdysteroid 22-kinase activity

TAS
PMID:32540344
Genomic and transcriptomic analyses in Drosophila suggest th...

UNDECIDED

Summary: The cited study proposes family-wide detoxification-by-phosphorylation hypotheses from genomic and expression analyses. Its accessible abstract does not establish CG31099-catalyzed phosphorylation of an ecdysteroid specifically at position 22. The kinase-like family assignment supports a candidate catalytic role, but exact substrate and positional specificity require target-specific or well-resolved subfamily evidence.

Reason: The cited study proposes family-wide detoxification-by-phosphorylation hypotheses from genomic and expression analyses. Its accessible abstract does not establish CG31099-catalyzed phosphorylation of an ecdysteroid specifically at position 22. The kinase-like family assignment supports a candidate catalytic role, but exact substrate and positional specificity require target-specific or well-resolved subfamily evidence.

Supporting Evidence:

PMID:32540344

We propose the hypothesis that members of the arthropod-specific ecdysteroid kinase-like (EcKL) gene family encode detoxicative kinases.

file:DROME/CG31099/CG31099-uniprot.txt

/note="CHK kinase-like"

PMID:38291829

Our results show the only two genes known to encode ecdysteroid kinases belong to different subfamilies and therefore ecdysteroid metabolism functions must be spread throughout the EcKL family.

External Prediction Reviews

These computational predictions are reviewed separately from the GOA annotation set used for this review. The assessments below are from this project and do not constitute official GO annotations or endorsement by GO/UniProt. They are not included in the existing annotation review above.

ProtNLM2 External predictions

View prediction review YAML · CG31099-protnlm-predictions-review.yaml · Review status: COMPLETE

Phosphorylation is plausible for this EcKL/CHK-kinase-like protein, but the available family study proposes rather than demonstrates the relevant reactions across this diverse family.

Source documents: genes/DROME/CG31099/CG31099-predictions-source.json · genes/DROME/CG31099/CG31099-uniprot-source.json

Review score: 2 = concordant with evidence; 1 = uncertain; 0 = discordant with evidence. This is an assessment score, not a model probability.

GO:0016310 phosphorylation GO_BP

UNC — Uncertain Review score: 1/2

Prediction method: ProtNLM2 · Version: UniProt API snapshot 2026-09-08 · file:DROME/CG31099/CG31099-predictions-source.json

Review rationale: Phosphorylation is plausible for this EcKL/CHK-kinase-like protein, but the available family study proposes rather than demonstrates the relevant reactions across this diverse family. No CG31099 substrate, product or catalytic assay is established in the accessible evidence, and the existing ecdysteroid 22-kinase TAS annotation is itself unresolved. Domain nomenclature and agreement with that annotation therefore cannot independently validate the process, although there is no evidence refuting it.

Supporting Evidence:

PMID:32540344: "We propose the hypothesis that members of the arthropod-specific ecdysteroid kinase-like (EcKL) gene family encode detoxicative kinases."
file:DROME/CG31099/CG31099-uniprot.txt: "/note="CHK kinase-like""
PMID:38291829: "Our results show the only two genes known to encode ecdysteroid kinases belong to different subfamilies and therefore ecdysteroid metabolism functions must be spread throughout the EcKL family."

Gene Description

Existing Annotations Review

References

External Prediction Reviews

ProtNLM2 External predictions

Deep Research

Falcon

📚 Additional Documentation

Notes

📄 View Raw YAML