CG31606

These computational predictions are reviewed separately from the GOA annotation set used for this review. The assessments below are from this project and do not constitute official GO annotations or endorsement by GO/UniProt. They are not included in the existing annotation review above.

GO Term	Evidence	Action	Reason
GO:0003674 molecular_function	ND GO_REF:0000015	ACCEPT	Summary: Retain the ND root annotation: no molecular mechanism or physiological process is established for this uncharacterized protein. The signal peptide suggests secretion but does not identify a transported ligand, enzymatic substrate or role in lipid metabolism. Reason: Retain the ND root annotation: no molecular mechanism or physiological process is established for this uncharacterized protein. The signal peptide suggests secretion but does not identify a transported ligand, enzymatic substrate or role in lipid metabolism. Supporting Evidence: file:DROME/CG31606/CG31606-uniprot.txt SIGNAL 1..18
GO:0005575 cellular_component	ND GO_REF:0000015	MODIFY	Summary: A cleavable N-terminal signal peptide followed by a soluble mature sequence supports secretion as the best current localization inference. There is no membrane anchor or canonical ER-retention terminus. Extracellular region is a reasonable broad prediction; this is sequence-based inference rather than direct localization evidence. Reason: A cleavable N-terminal signal peptide followed by a soluble mature sequence supports secretion as the best current localization inference. There is no membrane anchor or canonical ER-retention terminus. Extracellular region is a reasonable broad prediction; this is sequence-based inference rather than direct localization evidence. Proposed replacements: extracellular region Supporting Evidence: file:DROME/CG31606/CG31606-uniprot.txt SIGNAL 1..18 file:DROME/CG31606/CG31606-bioinformatics/RESULTS.md The C-terminal sequence is EEIASRILALKDNYQCA, without a hydrophobic anchor or canonical KDEL/HDEL retention terminus.
GO:0008150 biological_process	ND GO_REF:0000015	ACCEPT	Summary: Retain the ND root annotation: no molecular mechanism or physiological process is established for this uncharacterized protein. The signal peptide suggests secretion but does not identify a transported ligand, enzymatic substrate or role in lipid metabolism. Reason: Retain the ND root annotation: no molecular mechanism or physiological process is established for this uncharacterized protein. The signal peptide suggests secretion but does not identify a transported ligand, enzymatic substrate or role in lipid metabolism. Supporting Evidence: file:DROME/CG31606/CG31606-uniprot.txt SIGNAL 1..18

ProtNLM2 External predictions

View prediction review YAML · CG31606-protnlm-predictions-review.yaml · Review status: COMPLETE

Secretion is supported by sequence topology; lipid transport, lipid binding and lipoprotein metabolism remain unresolved.

Source documents: genes/DROME/CG31606/CG31606-predictions-source.json · genes/DROME/CG31606/CG31606-uniprot-source.json

Review score: 2 = concordant with evidence; 1 = uncertain; 0 = discordant with evidence. This is an assessment score, not a model probability.

GO:0006869 lipid transport GO_BP

UNC — Uncertain Review score: 1/2

Prediction method: ProtNLM2 · Version: UniProt API snapshot 2026-09-08 · file:DROME/CG31606/CG31606-predictions-source.json

Review rationale: Lipid transport is not established for CG31606. The raw structural donor is mammalian apolipoprotein A-V, but the strongly asymmetric TM scores do not establish target orthology or a conserved lipid-carrying mechanism. A signal peptide supports secretion rather than cargo specificity. Target lipid transport or convincing subfamily evidence is missing, and the claim is neither validated nor refuted.

Supporting Evidence:

file:DROME/CG31606/CG31606-uniprot.txt: "SIGNAL 1..18"
file:DROME/CG31606/CG31606-bioinformatics/RESULTS.md: "The lipid-function donor P0DTS2 is a 367-residue northern elephant seal apolipoprotein A-V. The two supplied TM scores are 0.50614 and 0.30402."

GO:0005576 extracellular region GO_CC

COR — Correct novel prediction Review score: 2/2

Prediction method: ProtNLM2 · Version: UniProt API snapshot 2026-09-08 · file:DROME/CG31606/CG31606-predictions-source.json

Review rationale: Extracellular localization is a reasonable inference from the cleavable N-terminal signal peptide and soluble mature sequence lacking a membrane anchor or canonical ER-retention terminus. It is not an experimentally measured compartment, and the bacterial IpgD donor is not its biological justification. This broad localization is absent from the ND-only GOA record, so it is a supported novel sequence-based prediction. It does not establish extracellular-matrix residence or lipid handling.

Supporting Evidence:

file:DROME/CG31606/CG31606-uniprot.txt: "SIGNAL 1..18"
file:DROME/CG31606/CG31606-bioinformatics/RESULTS.md: "The C-terminal sequence is EEIASRILALKDNYQCA, without a hydrophobic anchor or canonical KDEL/HDEL retention terminus."

GO:0008289 lipid binding GO_MF

UNC — Uncertain Review score: 1/2

Prediction method: ProtNLM2 · Version: UniProt API snapshot 2026-09-08 · file:DROME/CG31606/CG31606-predictions-source.json

Review rationale: No lipid-binding assay or defensible apolipoprotein subfamily placement is available for CG31606. The partial structural match to seal apolipoprotein A-V is a hypothesis lead rather than conserved ligand-binding evidence. Secretion does not identify a ligand. The exact binding claim remains unresolved.

Supporting Evidence:

file:DROME/CG31606/CG31606-bioinformatics/RESULTS.md: "The lipid-function donor P0DTS2 is a 367-residue northern elephant seal apolipoprotein A-V. The two supplied TM scores are 0.50614 and 0.30402."
file:DROME/CG31606/CG31606-uniprot.txt: "SIGNAL 1..18"

GO:0042157 lipoprotein metabolic process GO_BP

UNC — Uncertain Review score: 1/2

Prediction method: ProtNLM2 · Version: UniProt API snapshot 2026-09-08 · file:DROME/CG31606/CG31606-predictions-source.json

Review rationale: No evidence connects CG31606 to lipoprotein metabolism. The structurally matched apolipoprotein donor is not established as an ortholog, and a predicted signal peptide does not identify a pathway. Target perturbation, biochemical context or sound comparative functional transfer is needed to evaluate this process.

Supporting Evidence:

file:DROME/CG31606/CG31606-bioinformatics/RESULTS.md: "The lipid-function donor P0DTS2 is a 367-residue northern elephant seal apolipoprotein A-V. The two supplied TM scores are 0.50614 and 0.30402."
file:DROME/CG31606/CG31606-uniprot.txt: "SIGNAL 1..18"

Gene Description

Existing Annotations Review

References

External Prediction Reviews

ProtNLM2 External predictions

Deep Research

Falcon

📚 Additional Documentation

Notes

Bioinformatics Results

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