CG34171

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0004252 serine-type endopeptidase activity	IKR NOT PMID:30367934 Building a platform for predicting functions of serine prote...	ACCEPT	Summary: Retain the explicit NOT annotation: the selected short product lacks the catalytic-serine region. Reason: This is a negated IKR annotation, not a positive activity assertion. The primary study classifies SPs versus SPHs by catalytic-triad conservation, and current sequence analysis independently supports the negative conclusion for X2JEK1: H72/D124 are retained but the aligned catalytic-serine region is absent. The long CG34171 product instead has F222 at the serine-equivalent position. The distinct isoform defects support absent canonical catalysis without treating the long protein sequence as the target or implying a direct negative enzyme assay. Supporting Evidence: file:DROME/CG34171/CG34171-bioinformatics/RESULTS.md X2JEK1 preserves H72 and D124 but lacks the aligned catalytic-serine region. A8DZ15 has F222 at the serine-equivalent position. PMID:30367934 based on the presence or absence of the His-Asp-Ser catalytic triad
GO:0006508 proteolysis	IEA GO_REF:0000002	UNDECIDED	Summary: Participation in proteolysis is unresolved despite the absence of intrinsic protease activity. Reason: The InterPro trypsin-domain mapping does not establish an intact catalytic mechanism in the short product. Nevertheless, a noncatalytic protease homolog can participate in a proteolytic cascade through a cofactor role, so loss of activity alone does not refute this biological-process annotation. No target-specific evidence establishes such participation, and gene-level SPH/immune-family context cannot determine the role of the short PC isoform. Supporting Evidence: file:DROME/CG34171/CG34171-bioinformatics/RESULTS.md X2JEK1 preserves H72 and D124 but lacks the aligned catalytic-serine region. A8DZ15 has F222 at the serine-equivalent position.

GO Term

Evidence

Action

Reason

GO:0004252 serine-type endopeptidase activity

IKR NOT
PMID:30367934
Building a platform for predicting functions of serine prote...

Summary: Retain the explicit NOT annotation: the selected short product lacks the catalytic-serine region.

Reason: This is a negated IKR annotation, not a positive activity assertion. The primary study classifies SPs versus SPHs by catalytic-triad conservation, and current sequence analysis independently supports the negative conclusion for X2JEK1: H72/D124 are retained but the aligned catalytic-serine region is absent. The long CG34171 product instead has F222 at the serine-equivalent position. The distinct isoform defects support absent canonical catalysis without treating the long protein sequence as the target or implying a direct negative enzyme assay.

Supporting Evidence:

file:DROME/CG34171/CG34171-bioinformatics/RESULTS.md

X2JEK1 preserves H72 and D124 but lacks the aligned catalytic-serine region. A8DZ15 has F222 at the serine-equivalent position.

PMID:30367934

based on the presence or absence of the His-Asp-Ser catalytic triad

GO:0006508 proteolysis

IEA
GO_REF:0000002

UNDECIDED

Summary: Participation in proteolysis is unresolved despite the absence of intrinsic protease activity.

Reason: The InterPro trypsin-domain mapping does not establish an intact catalytic mechanism in the short product. Nevertheless, a noncatalytic protease homolog can participate in a proteolytic cascade through a cofactor role, so loss of activity alone does not refute this biological-process annotation. No target-specific evidence establishes such participation, and gene-level SPH/immune-family context cannot determine the role of the short PC isoform.

Supporting Evidence:

file:DROME/CG34171/CG34171-bioinformatics/RESULTS.md

X2JEK1 preserves H72 and D124 but lacks the aligned catalytic-serine region. A8DZ15 has F222 at the serine-equivalent position.

External Prediction Reviews

These computational predictions are reviewed separately from the GOA annotation set used for this review. The assessments below are from this project and do not constitute official GO annotations or endorsement by GO/UniProt. They are not included in the existing annotation review above.

ProtNLM2 External predictions

View prediction review YAML · CG34171-protnlm-predictions-review.yaml · Review status: COMPLETE

The predicted serine-type endopeptidase activity is contradicted by the absence of the catalytic-serine region in the selected short CG34171 isoform.

Source documents: projects/PROTNLM_EVALUATION/fly-benchmark/predictions.jsonl.gz · genes/DROME/CG34171/CG34171-predictions-source.json · genes/DROME/CG34171/CG34171-bioinformatics/RESULTS.md

Review score: 2 = concordant with evidence; 1 = uncertain; 0 = discordant with evidence. This is an assessment score, not a model probability.

GO:0004252 serine-type endopeptidase activity GO_MF

NPI — Nonparalog incorrect Review score: 0/2

Prediction method: ProtNLM2 · Version: UniProt API snapshot 2026-09-08

DOMAIN ARCHITECTURE MISMATCH

Review rationale: X2JEK1 is the 176-residue CG34171-PC isoform, not the 292-residue PB product. Two alignment modes and two active S1-domain references place H72 and D124 at conserved catalytic positions, but the entire catalytic-serine region is absent from the short product. The longer product has F222 at that position; that is a separate substitution and must not be assigned to the missing short-product sequence. This contradicts the predicted intrinsic activity and agrees with the explicit NOT IKR annotation to the same term. The raw prediction records Q66TN7 as its donor at model score 0.99, but its hit coordinates and historical input sequence are unavailable. The decisive evidence is the reproduced catalytic-region defect, not agreement with a database label or the SPH synonym.

Supporting Evidence:

file:DROME/CG34171/CG34171-bioinformatics/RESULTS.md: "X2JEK1 preserves H72 and D124 but lacks the aligned catalytic-serine region. A8DZ15 has F222 at the serine-equivalent position."
PMID:30367934: "based on the presence or absence of the His-Asp-Ser catalytic triad"

📚 Additional Documentation

Bioinformatics Results

(RESULTS.md)

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Gene Description

Existing Annotations Review

References

External Prediction Reviews

ProtNLM2 External predictions

Deep Research

Falcon

📚 Additional Documentation

Notes

Bioinformatics Results

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