id: X2JEK1
gene_symbol: CG34171
taxon:
  id: NCBITaxon:7227
  label: Drosophila melanogaster
status: COMPLETE
description: The predicted serine-type endopeptidase activity is contradicted by the absence of the catalytic-serine
  region in the selected short CG34171 isoform.
source_documents:
- projects/PROTNLM_EVALUATION/fly-benchmark/predictions.jsonl.gz
- genes/DROME/CG34171/CG34171-predictions-source.json
- genes/DROME/CG34171/CG34171-bioinformatics/RESULTS.md
references:
- id: PMID:30367934
  title: Building a platform for predicting functions of serine protease-related proteins in Drosophila
    melanogaster and other insects.
  findings:
  - statement: The study distinguishes serine proteases from their noncatalytic homologs by catalytic-triad
      conservation; the exact supplementary sequence for SPH244 was not retrieved.
    supporting_text: based on the presence or absence of the His-Asp-Ser catalytic triad
  full_text_unavailable: false
  reference_review:
    relevance: HIGH
    correctness: VERIFIED
    review_notes: PubMed metadata and cached full text match the cited study. The methods support the
      IKR basis; the X2JEK1-specific residue/coverage assessment is independently reproduced from current
      records. The inaccessible Table S1 was not claimed as inspected.
- id: file:DROME/CG34171/CG34171-bioinformatics/RESULTS.md
  title: CG34171 isoform and catalytic-site analysis
  findings: []
predictions:
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-09-08
  predicted_term:
    id: GO:0004252
    label: serine-type endopeptidase activity
  predicted_term_type: GO_MF
  review:
    assessment: NPI
    error_type: DOMAIN_ARCHITECTURE_MISMATCH
    confidence_score: 0
    summary: X2JEK1 is the 176-residue CG34171-PC isoform, not the 292-residue PB product. Two alignment
      modes and two active S1-domain references place H72 and D124 at conserved catalytic positions, but
      the entire catalytic-serine region is absent from the short product. The longer product has F222
      at that position; that is a separate substitution and must not be assigned to the missing short-product
      sequence. This contradicts the predicted intrinsic activity and agrees with the explicit NOT IKR
      annotation to the same term. The raw prediction records Q66TN7 as its donor at model score 0.99,
      but its hit coordinates and historical input sequence are unavailable. The decisive evidence is
      the reproduced catalytic-region defect, not agreement with a database label or the SPH synonym.
    supported_by:
    - reference_id: file:DROME/CG34171/CG34171-bioinformatics/RESULTS.md
      supporting_text: X2JEK1 preserves H72 and D124 but lacks the aligned catalytic-serine region. A8DZ15
        has F222 at the serine-equivalent position.
    - reference_id: PMID:30367934
      supporting_text: based on the presence or absence of the His-Asp-Ser catalytic triad