CG42763 encodes a small (191-residue) uncharacterised protein that is restricted to the Drosophila melanogaster species group. It has a predicted cleavable N-terminal signal peptide (residues 1-22) followed by a soluble mature chain with no transmembrane segment, no ER-retention signal and no recognised protein family or domain, which suggests that the protein is secreted. Full-length orthologs are found only in melanogaster-group drosophilids, and the only detectable D. melanogaster relative is the equally uncharacterised CG31606, which matches over part of the sequence at a marginal E-value. The gene is transcribed, but its molecular activity, binding partners, tissue of action and physiological role have not been studied.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0003674 molecular_function | ND GO_REF:0000015 | ACCEPT | Summary: No molecular function is known. The ND root annotation correctly records the absence of evidence. Reason: PubMed and Europe PMC searches for the gene name and FlyBase identifier return no publications, so no gene-specific functional study exists. The protein has no InterPro or PANTHER signature and no characterised relative from which an activity could be inferred. Its only D. melanogaster relative, CG31606, is uncharacterised and matches only partially at a marginal E-value. A signal peptide shows entry into the secretory pathway but says nothing about molecular activity. Supporting Evidence: file:DROME/CG42763/CG42763-notes.md PubMed (esearch "CG42763": 0 records) and Europe PMC (full-text queries file:DROME/CG42763/CG42763-bioinformatics/RESULTS.md InterPro returns no entry matches for M9MRG9 (neither integrated InterPro entries nor member-database signatures), and UniProt lists no PANTHER family. |
| GO:0005575 cellular_component | ND GO_REF:0000015 | ACCEPT | Summary: Root cellular component ND annotation: when it was made, no cellular component had been curated for this gene product from literature. Reason: ACCEPT under the ND acceptance rule (projects/NO_DATA.md). The evidence this review cites for the cellular component is inferred (from orthologs, family membership or sequence features) or comes from other species; none of it is literature on this gene product in this species. This was judged from the review's own content during the NO_DATA re-action pass, not from a new literature search. Earlier action: MODIFY. The term the earlier MODIFY proposed, extracellular region, is kept as its own NEW row. The gene's own literature-search record finds no study of this gene product in this species that identifies a cellular location. Supporting Evidence: file:DROME/CG42763/CG42763-notes.md "FBgn0261831": 0 hits) found no publication that mentions the gene. |
| GO:0008150 biological_process | ND GO_REF:0000015 | ACCEPT | Summary: No biological process is known. The ND root annotation is appropriate. Reason: No perturbation, phenotype or expression study of this gene has been published. The gene is lineage-restricted and has no characterised ortholog or PANTHER/PAINT family, so no process can be inferred by homology. Supporting Evidence: file:DROME/CG42763/CG42763-bioinformatics/RESULTS.md CG42763 is a taxonomically restricted gene of the *D. melanogaster* species group file:DROME/CG42763/CG42763-notes.md No function of CG42763 is experimentally established in D. |
| GO:0005576 extracellular region | ISM file:DROME/CG42763/CG42763-uniprot.txt | NEW | Summary: Inferred extracellular region for this gene product, proposed during the NO_DATA re-action pass. Reason: Extracellular region is inferred for this gene product (ISM), not shown for it experimentally; the ND row for the aspect therefore stays (projects/NO_DATA.md). The protein has a SignalP-predicted cleavable signal peptide (residues 1-22) followed by a soluble 169-residue mature chain. It has no transmembrane segment and no C-terminal KDEL/HDEL ER-retention motif, so the default fate is secretion. The broad term GO:0005576 extracellular region is as specific as this evidence allows. A more specific location, such as extracellular space or hemolymph, would need direct localisation data, and no experiment has measured where the protein is. Supporting Evidence: file:DROME/CG42763/CG42763-uniprot.txt SIGNAL 1..22 file:DROME/CG42763/CG42763-bioinformatics/RESULTS.md The C-terminal sequence is ILKYLTRFPSAKCTLPN, which has no hydrophobic anchor and no KDEL/HDEL ER-retention motif. |
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Download this section (compressed HTML)Q: Is CG42763 secreted into the hemolymph or a reproductive or gut lumen, and in which tissues and life stages is it expressed?
Q: Do CG42763 and CG31606 share a fold (for example by structure-prediction comparison), and did they arise by duplication within the melanogaster group?
Experiment: Express an epitope-tagged CG42763 under its native regulatory region and test whether the protein is secreted, and whether removing the signal peptide abolishes secretion.
Experiment: Make a CRISPR null allele and screen for viability, fertility, immune and metabolic phenotypes, alone and combined with a CG31606 null to test for redundancy.
What is not known — curated, literature-grounded statements of the open unknowns (the inverse of core functions).
Gap: The molecular activity, binding partners, site of expression and physiological role of the predicted secreted CG42763 protein are entirely undetermined.
OPEN BIOLOGY WHOLLY_DARK
What is known: The protein is a predicted secreted small protein with a signal peptide and no recognised domain. It is restricted to the melanogaster species group, and its only detectable paralog (CG31606) is also uncharacterised.
What would resolve it: Localisation of a tagged protein, a loss-of-function phenotype screen and identification of interaction partners.
Provenance (the field's own admissions):
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