CG4592 is one of a tandem cluster of three Drosophila melanogaster mitochondrial enoyl-CoA delta isomerase 1 paralogs (with CG4594 and CG4598), the mitochondrial-type (human ECI1 class) delta(3),delta(2)-enoyl-CoA isomerases (EC 5.3.3.8). It is an auxiliary enzyme of unsaturated fatty acid beta-oxidation that isomerizes both 3-cis (3Z) and 3-trans (3E) enoyl-CoA double bonds into the 2-trans (2E) form (with a preference for (3Z)-enoyl-CoAs), regenerating the substrate of the core enoyl-CoA hydratase so that unsaturated fatty acids with double bonds at odd-numbered positions can be fully degraded. It localizes to the mitochondrial matrix and, unlike the peroxisomal-type isomerase Dci, is the mitochondrial isomerase relevant to the mitochondrial unsaturated-FAO cassette. Among the three tandem paralogs, CG4592 currently carries the sparsest annotation set (electronic isomerase MF plus matrix localization only).
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0004165 delta(3)-delta(2)-enoyl-CoA isomerase activity | IEA GO_REF:0000003 | ACCEPT | Summary: EC-based electronic assignment of delta(3),delta(2)-enoyl-CoA isomerase activity (EC 5.3.3.8) - the defining molecular function of this mitochondrial ECI1-class isomerase. Reason: Core molecular function; concordant with the UniProt name and mitochondrial ECI1 family membership. Supporting Evidence: file:DROME/CG4592/CG4592-uniprot.txt Enoyl-CoA delta isomerase 1, mitochondrial |
| GO:0005759 mitochondrial matrix | IEA GO_REF:0000044 | ACCEPT | Summary: Electronic (UniProt subcellular-location) annotation to the mitochondrial matrix - the correct, specific compartment for this mitochondrial-type enoyl-CoA isomerase. Reason: The specific, core location; distinguishes CG4592 from the peroxisomal-type isomerase Dci. The record's SUBCELLULAR LOCATION line is ARBA-only and the entry has no FT features at all (no transit-peptide or transmembrane annotation), so the support is classification: PANTHER subfamily PTHR11941:SF45 (enoyl-CoA delta isomerase 1, mitochondrial) and FunFam 3.90.226.10:FF:000034 (enoyl-CoA delta isomerase 1). ECI1-subfamily isomerases are soluble mitochondrial matrix enzymes; this is a family-level inference, not a fly localization assay. Supporting Evidence: file:DROME/CG4592/CG4592-uniprot.txt PTHR11941:SF45; ENOYL-COA DELTA ISOMERASE 1, MITOCHONDRIAL file:DROME/CG4592/CG4592-uniprot.txt 3.90.226.10:FF:000034; Enoyl-CoA delta isomerase 1 |
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Download this section (compressed HTML)Q: Do the three tandem mitochondrial enoyl-CoA delta-isomerase paralogs (CG4592/CG4594/CG4598) have distinct expression or substrate roles, or are they functionally redundant copies of the mitochondrial delta(3),delta(2)-enoyl-CoA isomerase?
Experiment: Expression profiling and individual/combined knockouts of CG4592, CG4594 and CG4598 to test functional redundancy and confirm the mitochondrial delta(3),delta(2)-enoyl-CoA isomerase step of unsaturated fatty acid beta-oxidation in the fly.
Hypothesis: The CG4592/CG4594/CG4598 cluster provides the mitochondrial delta(3),delta(2)-enoyl-CoA isomerase activity (distinct from peroxisomal Dci) required for the mitochondrial unsaturated-FAO cassette.
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