CG8353 is a compact CDA-like zinc-dependent deaminase associated with pyrimidine nucleoside salvage. Its cytidine-deaminase family signature supports hydrolytic conversion of cytidine and deoxycytidine to the corresponding uridine nucleosides. Cytosolic localization and the reaction assignment are evolutionary inferences; direct fly substrate kinetics and oligomerization measurements are not established.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0004126 cytidine deaminase activity | IBA GO_REF:0000033 | ACCEPT | Summary: Cytidine deamination is supported by the CDA-like branch and compact enzyme architecture. Reason: The 170-residue target has a complete cytidine-deaminase-like zinc-binding domain and the tetrameric cytidine-deaminase family signature. These agree with the curated PAINT inference of free-nucleoside deamination; the broad CMP/dCMP-type domain label alone would not resolve substrate specificity. Supporting Evidence: file:DROME/CG8353/CG8353-uniprot.txt DR InterPro; IPR006262; Cyt_deam_tetra. file:DROME/CG8353/CG8353-uniprot.txt DR InterPro; IPR016192; APOBEC/CMP_deaminase_Zn-bd. |
| GO:0004126 cytidine deaminase activity | IEA GO_REF:0000120 | ACCEPT | Summary: Cytidine deamination is supported by the CDA-like branch and compact enzyme architecture. Reason: The 170-residue target has a complete cytidine-deaminase-like zinc-binding domain and the tetrameric cytidine-deaminase family signature. These agree with the curated PAINT inference of free-nucleoside deamination; the broad CMP/dCMP-type domain label alone would not resolve substrate specificity. Supporting Evidence: file:DROME/CG8353/CG8353-uniprot.txt DR InterPro; IPR006262; Cyt_deam_tetra. file:DROME/CG8353/CG8353-uniprot.txt DR InterPro; IPR016192; APOBEC/CMP_deaminase_Zn-bd. |
| GO:0005737 cytoplasm | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Cytoplasm is compatible with the more specific cytosolic inference. Reason: The compact soluble CDA-like architecture lacks a membrane topology, and the PAINT location is cytosol. This broad compartment does not add evidence of a particular cellular microdomain. Supporting Evidence: file:DROME/CG8353/CG8353-uniprot.txt DR InterPro; IPR006262; Cyt_deam_tetra. |
| GO:0005829 cytosol | IBA GO_REF:0000033 | ACCEPT | Summary: Cytosolic pyrimidine salvage is a reasonable inherited location. Reason: The target is a compact soluble CDA-like enzyme with the conserved cytidine-deaminase classification. The phylogenetic cytosolic placement is retained; direct fly localization is not available. Supporting Evidence: file:DROME/CG8353/CG8353-uniprot.txt DR InterPro; IPR006262; Cyt_deam_tetra. |
| GO:0006217 deoxycytidine catabolic process | IBA GO_REF:0000033 | ACCEPT | Summary: Deoxycytidine catabolism fits the cytidine-deaminase branch. Reason: CDA-type nucleoside deaminases act on cytidine and deoxycytidine. The PAINT reaction-level process is compatible with the specific tetrameric CDA signature and does not rely on the ARBA functional sentence. Supporting Evidence: file:DROME/CG8353/CG8353-uniprot.txt DR InterPro; IPR006262; Cyt_deam_tetra. |
| GO:0008270 zinc ion binding | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: Zinc is a catalytic cofactor of the deaminase fold. Reason: The target contains the conserved zinc-binding deaminase domain. Metal binding is ancillary to hydrolytic deamination, not a separate zinc-homeostasis activity. Supporting Evidence: file:DROME/CG8353/CG8353-uniprot.txt DR InterPro; IPR016192; APOBEC/CMP_deaminase_Zn-bd. |
| GO:0016787 hydrolase activity | IEA GO_REF:0000002 | MODIFY | Summary: Cytidine deaminase specifies the generic hydrolase class. Reason: The CDA-like branch supports hydrolytic removal of the cytidine amino group, so the substrate-defined enzyme term carries the useful function. Proposed replacements: cytidine deaminase activity Supporting Evidence: file:DROME/CG8353/CG8353-uniprot.txt DR InterPro; IPR006262; Cyt_deam_tetra. |
| GO:0042802 identical protein binding | IEA GO_REF:0000117 | UNDECIDED | Summary: The target oligomeric interaction is not directly established. Reason: The tetrameric CDA family signature suggests self-association, but sequence classification does not measure oligomerization of CG8353. The broad identical-protein-binding term adds little mechanistic specificity without assembly data. Supporting Evidence: file:DROME/CG8353/CG8353-uniprot.txt DR InterPro; IPR006262; Cyt_deam_tetra. |
| GO:0046109 uridine biosynthetic process | IBA GO_REF:0000033 | ACCEPT | Summary: Uridine production follows cytidine deamination. Reason: The inherited uridine-biosynthesis annotation expresses the direct product of the cytidine-deaminase reaction. This does not imply that the enzyme supplies all steps of de-novo pyrimidine synthesis. Supporting Evidence: file:DROME/CG8353/CG8353-uniprot.txt DR InterPro; IPR006262; Cyt_deam_tetra. |
| GO:0055086 nucleobase-containing small molecule metabolic process | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Nucleobase-containing small molecule metabolic process is a broad parent of nucleoside salvage. Reason: The supported CDA-like reaction acts on pyrimidine nucleosides. This parent process is biologically compatible but less informative than the direct cytidine/deoxycytidine deamination processes. Supporting Evidence: file:DROME/CG8353/CG8353-uniprot.txt DR InterPro; IPR006262; Cyt_deam_tetra. |
| GO:0072527 pyrimidine-containing compound metabolic process | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Pyrimidine-containing compound metabolic process is a broad parent of nucleoside salvage. Reason: The supported CDA-like reaction acts on pyrimidine nucleosides. This parent process is biologically compatible but less informative than the direct cytidine/deoxycytidine deamination processes. Supporting Evidence: file:DROME/CG8353/CG8353-uniprot.txt DR InterPro; IPR006262; Cyt_deam_tetra. |
| GO:1901135 carbohydrate derivative metabolic process | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Carbohydrate derivative metabolic process is a broad parent of nucleoside salvage. Reason: The supported CDA-like reaction acts on pyrimidine nucleosides. This parent process is biologically compatible but less informative than the direct cytidine/deoxycytidine deamination processes. Supporting Evidence: file:DROME/CG8353/CG8353-uniprot.txt DR InterPro; IPR006262; Cyt_deam_tetra. |
Loading supporting content…
Download this section (compressed HTML)Loading supporting content…
Download this section (compressed HTML)Loading supporting content…
Download this section (compressed HTML)