Cullin-1 (Cul1, lin-19) is the scaffold subunit of SCF (Skp1-Cul1-F-box) cullin-RING ubiquitin ligases in Drosophila. Its elongated N-terminal cullin-repeat domain binds the SkpA adaptor and thereby F-box substrate receptors (Slimb, Archipelago, Nutcracker, Fsn, Fbxo42, FipoQ and others), while its C-terminal cullin homology domain binds the RING protein Roc1a, which recruits the E2. Nedd8 conjugation to a conserved C-terminal lysine activates the ligase, and the COP9 signalosome removes it. SCF complexes built on Cul1 ubiquitinate phosphorylated substrates such as Cubitus interruptus, Armadillo, Cyclin E, Expanded and Akt, targeting them for proteasomal degradation or partial processing, and thereby act in Hedgehog, Wingless, Hippo, InR/TOR and IMD signaling, cell-cycle control, caspase activation during spermatid individualization, dendrite pruning and meiosis. In the auditory Johnston's organ, SCF activity built on Cul1, which the E3 ligase Ubr3 keeps in check, is needed for apical attachment of the scolopidia, and both RNAi knockdown and overexpression of Cul1 cause scolopidial detachment. Cul1 is found in the cytoplasm and nucleus.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0000209 protein polyubiquitination | IEA GO_REF:0000117 | ACCEPT | Summary: Protein polyubiquitination (ARBA). Reason: Cul1 is the scaffold of SCF ligases that polyubiquitinate substrates (e.g. K11-linked chains on Ci); a direct process of the assembled ligase. |
| GO:0002785 negative regulation of antimicrobial peptide production | IMP PMID:12401167 A ubiquitin-proteasome pathway represses the Drosophila immu... | KEEP AS NON CORE | Summary: Repression of IMD antimicrobial peptide expression. Reason: Cul1 mutations induce Diptericin; output of SCF-dependent Relish control. Non-core. Supporting Evidence: PMID:12401167 mutations affecting either the Drosophila SCF components, Slimb and dCullin1, or the proteasome also induce Diptericin expression |
| GO:0005515 protein binding | IPI PMID:17099689 Molecular dissection of the APC/C inhibitor Rca1 shows a nov... | MODIFY | Summary: Binding to SkpA. Reason: Protein binding is uninformative; the Cul1-SkpA interaction is the scaffolding of the SCF, captured by GO:0160072 ubiquitin ligase complex scaffold activity. Proposed replacements: ubiquitin ligase complex scaffold activity |
| GO:0005515 protein binding | IPI PMID:17099689 Molecular dissection of the APC/C inhibitor Rca1 shows a nov... | MODIFY | Summary: Co-complex with the F-box protein Rca1. Reason: Protein binding is uninformative; Cul1 associates with F-box proteins via SkpA as the SCF scaffold, captured by GO:0160072 ubiquitin ligase complex scaffold activity. Proposed replacements: ubiquitin ligase complex scaffold activity Supporting Evidence: PMID:17099689 We propose that Rca1 acts as an F-box protein in an as yet uncharacterized SCF complex, which promotes S-phase entry. |
| GO:0005515 protein binding | IPI PMID:19723762 Drosophila Minus is required for cell proliferation and infl... | REMOVE | Summary: Binding to Minus. Reason: Minus associates with SCF(Ago) and Cyclin E; the bare protein binding term is uninformative and no specific Cul1 binding activity for Minus is established. Removed. |
| GO:0005634 nucleus | IBA GO_REF:0000033 | ACCEPT | Summary: Nucleus. Reason: Cullins act in nuclear and cytoplasmic CRLs. IBA from PTN002631076 (donors include human CUL1-4 and multiple MODs). |
| GO:0005634 nucleus | IDA PMID:12737805 The COP9 signalosome promotes degradation of Cyclin E during... | ACCEPT | Summary: Nucleus (IDA, CSN/Cyclin E oogenesis paper). Reason: Abstract-only cache does not describe Cul1 subcellular localization; curators read the full text. Nuclear cullin activity is expected (Cyclin E degradation); accepted deferring to the curator. |
| GO:0005737 cytoplasm | EXP PMID:20392747 A novel F-box protein is required for caspase activation dur... | ACCEPT | Summary: Cytoplasm. Reason: Cul1-based SCF complexes act on cytoplasmic substrates (Ci-155, Arm). The EXP row is from the Nutcracker paper (full text cached but no explicit Cul1 localization statement found); accepted deferring to the curator. |
| GO:0005737 cytoplasm | IEA GO_REF:0000044 | ACCEPT | Summary: Cytoplasm. Reason: Cul1-based SCF complexes act on cytoplasmic substrates (Ci-155, Arm). The EXP row is from the Nutcracker paper (full text cached but no explicit Cul1 localization statement found); accepted deferring to the curator. |
| GO:0005829 cytosol | IEA GO_REF:0000117 | ACCEPT | Summary: Cytosol. Reason: Reactome places the fly SCF(Slimb) Arm and Ci ubiquitination reactions in the cytosol; consistent with cytoplasmic substrates. Acceptable. |
| GO:0005829 cytosol | TAS Reactome:R-DME-209094 | ACCEPT | Summary: Cytosol. Reason: Reactome places the fly SCF(Slimb) Arm and Ci ubiquitination reactions in the cytosol; consistent with cytoplasmic substrates. Acceptable. |
| GO:0005829 cytosol | TAS Reactome:R-DME-209102 | ACCEPT | Summary: Cytosol. Reason: Reactome places the fly SCF(Slimb) Arm and Ci ubiquitination reactions in the cytosol; consistent with cytoplasmic substrates. Acceptable. |
| GO:0005829 cytosol | TAS Reactome:R-DME-209224 | ACCEPT | Summary: Cytosol. Reason: Reactome places the fly SCF(Slimb) Arm and Ci ubiquitination reactions in the cytosol; consistent with cytoplasmic substrates. Acceptable. |
| GO:0005829 cytosol | TAS Reactome:R-DME-209278 | ACCEPT | Summary: Cytosol. Reason: Reactome places the fly SCF(Slimb) Arm and Ci ubiquitination reactions in the cytosol; consistent with cytoplasmic substrates. Acceptable. |
| GO:0006511 ubiquitin-dependent protein catabolic process | IEA GO_REF:0000002 | MODIFY | Summary: Ubiquitin-dependent protein catabolic process. Reason: Correct but less specific than the evidence supports; Cul1 acts in SCF ligases whose substrates are degraded by the proteasome, so MODIFY to GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process (a term Cul1 already carries). Proposed replacements: SCF-dependent proteasomal ubiquitin-dependent protein catabolic process |
| GO:0010564 regulation of cell cycle process | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Regulation of cell cycle process (ARBA). Reason: Broad but valid: SCF-Cul1 degrades Cyclin E and other cell-cycle regulators. Non-core. |
| GO:0016567 protein ubiquitination | IBA GO_REF:0000033 | MODIFY | Summary: Protein ubiquitination. Reason: Correct but less specific than the evidence supports: Cul1 acts in a cullin-RING ligase that polyubiquitinates its substrates. Per the batch rule for correct-but-general terms, MODIFY to GO:0000209 protein polyubiquitination. Proposed replacements: protein polyubiquitination |
| GO:0016567 protein ubiquitination | IC PMID:18698375 Identifying determinants of cullin binding specificity among... | MODIFY | Summary: Protein ubiquitination. Reason: Correct but less specific than the evidence supports: Cul1 acts in a cullin-RING ligase that polyubiquitinates its substrates. Per the batch rule for correct-but-general terms, MODIFY to GO:0000209 protein polyubiquitination. Proposed replacements: protein polyubiquitination Supporting Evidence: PMID:18698375 we show that Drosophila Roc proteins bind specific Cullins: Roc1a binds Cul1-4 |
| GO:0016567 protein ubiquitination | IC PMID:24068890 A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K... | MODIFY | Summary: Protein ubiquitination. Reason: Correct but less specific than the evidence supports: Cul1 acts in a cullin-RING ligase that polyubiquitinates its substrates. Per the batch rule for correct-but-general terms, MODIFY to GO:0000209 protein polyubiquitination. Proposed replacements: protein polyubiquitination Supporting Evidence: PMID:24068890 the F-box protein Slimb forms a complex with Akt, an activator of the InR/PI3K/TOR pathway, and promotes Akt ubiquitination |
| GO:0016567 protein ubiquitination | IDA PMID:26120032 Deubiquitination of Ci/Gli by Usp7/HAUSP Regulates Hedgehog ... | MODIFY | Summary: Protein ubiquitination. Reason: Correct but less specific than the evidence supports: Cul1 acts in a cullin-RING ligase that polyubiquitinates its substrates. Per the batch rule for correct-but-general terms, MODIFY to GO:0000209 protein polyubiquitination. Proposed replacements: protein polyubiquitination Supporting Evidence: PMID:26120032 which targets Ci ubiquitination mediated by Slimb-Cul1 E3 ligase |
| GO:0016567 protein ubiquitination | IDA PMID:32467643 Dual functions of Rack1 in regulating Hedgehog pathway. | MODIFY | Summary: Protein ubiquitination. Reason: Correct but less specific than the evidence supports: Cul1 acts in a cullin-RING ligase that polyubiquitinates its substrates. Per the batch rule for correct-but-general terms, MODIFY to GO:0000209 protein polyubiquitination. Proposed replacements: protein polyubiquitination Supporting Evidence: PMID:32467643 the phosphorylated Ci protein is recognized and ubiquitinated by Slimb-Cul1 E3 ligase to partially degrade into a truncated form of Ci (Ci-75) |
| GO:0016567 protein ubiquitination | IEA GO_REF:0000041 | MODIFY | Summary: Protein ubiquitination. Reason: Correct but less specific than the evidence supports: Cul1 acts in a cullin-RING ligase that polyubiquitinates its substrates. Per the batch rule for correct-but-general terms, MODIFY to GO:0000209 protein polyubiquitination. Proposed replacements: protein polyubiquitination |
| GO:0019005 SCF ubiquitin ligase complex | IBA GO_REF:0000033 | ACCEPT | Summary: Cul1 is the cullin scaffold of SCF complexes. Reason: Well supported by fly co-IP and genetic data with SkpA, Roc1a and multiple F-box proteins. |
| GO:0019005 SCF ubiquitin ligase complex | IDA PMID:11500045 Occurrence of a putative SCF ubiquitin ligase complex in Dro... | ACCEPT | Summary: Cul1 is the cullin scaffold of SCF complexes. Reason: Well supported by fly co-IP and genetic data with SkpA, Roc1a and multiple F-box proteins. Supporting Evidence: PMID:11500045 We show that putative Drosophila SCF core subunits dSkpA and dRbx1 both interact directly with dCu11 and the F-box protein Slmb. |
| GO:0019005 SCF ubiquitin ligase complex | IDA PMID:17925225 Regulation of Ci-SCFSlimb binding, Ci proteolysis, and hedge... | ACCEPT | Summary: Cul1 is the cullin scaffold of SCF complexes. Reason: Well supported by fly co-IP and genetic data with SkpA, Roc1a and multiple F-box proteins. Supporting Evidence: PMID:17925225 When Hh is absent, phosphorylation of Ci/Gli triggers binding to SCF ubiquitin ligase complexes and consequent proteolysis. |
| GO:0019005 SCF ubiquitin ligase complex | IDA PMID:18698375 Identifying determinants of cullin binding specificity among... | ACCEPT | Summary: Cul1 is the cullin scaffold of SCF complexes. Reason: Well supported by fly co-IP and genetic data with SkpA, Roc1a and multiple F-box proteins. Supporting Evidence: PMID:18698375 we show that Drosophila Roc proteins bind specific Cullins: Roc1a binds Cul1-4 |
| GO:0019005 SCF ubiquitin ligase complex | IDA PMID:20123973 Regulation of Drosophila vasa in vivo through paralogous cul... | ACCEPT | Summary: SCF complex (Fsn/Gus Vasa paper). Reason: Abstract-only cache names Fsn as a Cullin-RING specificity receptor but does not mention Cul1; curators read the full text. Accepted deferring to the curator. |
| GO:0019005 SCF ubiquitin ligase complex | IDA PMID:23747190 Ter94 ATPase complex targets k11-linked ubiquitinated ci to ... | ACCEPT | Summary: Cul1 is the cullin scaffold of SCF complexes. Reason: Well supported by fly co-IP and genetic data with SkpA, Roc1a and multiple F-box proteins. Supporting Evidence: PMID:23747190 Ter94(Ufd1-like/dNpl4) complex interacts directly with Cul1-Slimb |
| GO:0019005 SCF ubiquitin ligase complex | IDA PMID:24068890 A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K... | ACCEPT | Summary: Cul1 is the cullin scaffold of SCF complexes. Reason: Well supported by fly co-IP and genetic data with SkpA, Roc1a and multiple F-box proteins. Supporting Evidence: PMID:24068890 The SCF E3 ligase consists of four core components Cullin1/Roc1a/SkpA/Slimb |
| GO:0019005 SCF ubiquitin ligase complex | IDA PMID:33382409 SCF-Fbxo42 promotes synaptonemal complex assembly by downreg... | ACCEPT | Summary: Cul1 is the cullin scaffold of SCF complexes. Reason: Well supported by fly co-IP and genetic data with SkpA, Roc1a and multiple F-box proteins. Supporting Evidence: PMID:33382409 It consists of three core subunits (Roc, Cul1, and Skp1) and one variable subunit (an F-box protein |
| GO:0019005 SCF ubiquitin ligase complex | IEA GO_REF:0000117 | ACCEPT | Summary: Cul1 is the cullin scaffold of SCF complexes. Reason: Well supported by fly co-IP and genetic data with SkpA, Roc1a and multiple F-box proteins. |
| GO:0019005 SCF ubiquitin ligase complex | IMP PMID:22359584 Defining components of the Γ-catenin destruction complex and... | ACCEPT | Summary: Cul1 is the cullin scaffold of SCF complexes. Reason: Well supported by fly co-IP and genetic data with SkpA, Roc1a and multiple F-box proteins. Supporting Evidence: PMID:22359584 Using RNAi in Drosophila S2 cells to examine which SCF components are essential for Armadillo destruction |
| GO:0019005 SCF ubiquitin ligase complex | IPI PMID:11500045 Occurrence of a putative SCF ubiquitin ligase complex in Dro... | ACCEPT | Summary: Cul1 is the cullin scaffold of SCF complexes. Reason: Well supported by fly co-IP and genetic data with SkpA, Roc1a and multiple F-box proteins. Supporting Evidence: PMID:11500045 We show that putative Drosophila SCF core subunits dSkpA and dRbx1 both interact directly with dCu11 and the F-box protein Slmb. |
| GO:0030163 protein catabolic process | IEA GO_REF:0000117 | MODIFY | Summary: Protein catabolic process. Reason: Correct but less specific than the evidence supports; Cul1 acts in SCF ligases whose substrates are degraded by the proteasome, so MODIFY to GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process (a term Cul1 already carries). Proposed replacements: SCF-dependent proteasomal ubiquitin-dependent protein catabolic process |
| GO:0030163 protein catabolic process | IMP PMID:24068890 A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K... | MODIFY | Summary: Protein catabolic process. Reason: Correct but less specific than the evidence supports; Cul1 acts in SCF ligases whose substrates are degraded by the proteasome, so MODIFY to GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process (a term Cul1 already carries). Proposed replacements: SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Supporting Evidence: PMID:24068890 the Cullin1-based E3 ligase facilitates ddaC dendrite pruning primarily through inactivation of the InR/PI3K/TOR pathway |
| GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process | IBA GO_REF:0000033 | ACCEPT | Summary: SCF-dependent proteasomal protein catabolism. Reason: Core process of the Cul1-based SCF ligases. |
| GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process | NAS PMID:11500045 Occurrence of a putative SCF ubiquitin ligase complex in Dro... | ACCEPT | Summary: SCF-dependent proteasomal protein catabolism. Reason: Core process of the Cul1-based SCF ligases. Supporting Evidence: PMID:11500045 Our results suggest that dRbx1, dSkpA, dCullin1, and Slimb proteins are components of a Drosophila SCF complex that functions in combination with the ubiquitin conjugating enzyme UbcD1. |
| GO:0031398 positive regulation of protein ubiquitination | IMP PMID:30685895 FipoQ/FBXO33, a Cullin-1-based ubiquitin ligase complex comp... | KEEP AS NON CORE | Summary: Positive regulation of protein ubiquitination (SCA3 polyQ). Reason: Cul1 knockdown alters ubiquitination and solubility of expanded SCA3; Cul1 is a structural part of the ligase rather than a regulator, so this is an indirect framing of its core activity. Non-core. Supporting Evidence: PMID:30685895 the knockdown of Cul1 expression enhances SCA3-induced neurodegeneration and reduces the solubility of expanded SCA3-polyQ proteins |
| GO:0031461 cullin-RING ubiquitin ligase complex | IEA GO_REF:0000002 | MODIFY | Summary: Cullin-RING ubiquitin ligase complex (InterPro). Reason: Correct but less specific than the evidence supports: Cul1 is specifically the cullin of SCF complexes, so MODIFY to GO:0019005 SCF ubiquitin ligase complex, which Cul1 already carries. Proposed replacements: SCF ubiquitin ligase complex |
| GO:0031625 ubiquitin protein ligase binding | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: Ubiquitin protein ligase binding. Reason: Cul1 binds the RING E3 subunit Roc1a; accurate structural binding descriptor but subsumed by its scaffold activity (GO:0160072). Non-core. |
| GO:0031625 ubiquitin protein ligase binding | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: Ubiquitin protein ligase binding. Reason: Cul1 binds the RING E3 subunit Roc1a; accurate structural binding descriptor but subsumed by its scaffold activity (GO:0160072). Non-core. |
| GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process | IMP PMID:22359584 Defining components of the Γ-catenin destruction complex and... | KEEP AS NON CORE | Summary: Positive regulation of proteasomal ubiquitin-dependent catabolism. Reason: The cullin-RING ligase containing Cul1 executes rather than regulates substrate degradation; the direct process is GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process. The regulation term is a common E3-ligase convention and is kept as non-core. Supporting Evidence: PMID:22359584 Using RNAi in Drosophila S2 cells to examine which SCF components are essential for Armadillo destruction |
| GO:0035331 negative regulation of hippo signaling | IMP PMID:25522691 SCF(Slmb) E3 ligase-mediated degradation of Expanded is inhi... | KEEP AS NON CORE | Summary: Negative regulation of Hippo signaling. Reason: Cul1 as SCF(Slimb) scaffold is needed for Expanded/Kibra degradation; non-core output of the shared core. Supporting Evidence: PMID:25522691 the SCF(Slmb) E3 ligase as a novel negative regulator of the Hippo pathway in Drosophila imaginal tissues |
| GO:0035331 negative regulation of hippo signaling | IMP PMID:33555257 Negative feedback couples Hippo pathway activation with Kibr... | KEEP AS NON CORE | Summary: Negative regulation of Hippo signaling. Reason: Cul1 as SCF(Slimb) scaffold is needed for Expanded/Kibra degradation; non-core output of the shared core. Supporting Evidence: PMID:33555257 Hpo signaling complex formation promotes Kib degradation via SCFSlimb-mediated ubiquitination |
| GO:0043066 negative regulation of apoptotic process | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Negative regulation of apoptosis. Reason: Reported for SCF/dRYBP-dependent maintenance of DIAP1; non-core physiological output. |
| GO:0043066 negative regulation of apoptotic process | IMP PMID:23979704 A novel dRYBP-SCF complex functions to inhibit apoptosis in ... | KEEP AS NON CORE | Summary: Negative regulation of apoptosis. Reason: Reported for SCF/dRYBP-dependent maintenance of DIAP1; non-core physiological output. Supporting Evidence: PMID:23979704 interacts both genetically and biochemically with the E3 ubiquitin ligase SKPA, dCULLIN, F-box (SCF) complex to synergistically inhibit apoptosis in Drosophila |
| GO:0045879 negative regulation of smoothened signaling pathway | IDA PMID:32467643 Dual functions of Rack1 in regulating Hedgehog pathway. | KEEP AS NON CORE | Summary: Negative regulation of Hedgehog signaling. Reason: Cul1-based SCF(Slimb) processes Ci; for the shared cullin scaffold this pathway output is non-core (it is accepted as a defining role for Slimb). Supporting Evidence: PMID:32467643 the phosphorylated Ci protein is recognized and ubiquitinated by Slimb-Cul1 E3 ligase to partially degrade into a truncated form of Ci (Ci-75) |
| GO:0045879 negative regulation of smoothened signaling pathway | IMP PMID:12231629 Distinct protein degradation mechanisms mediated by Cul1 and... | KEEP AS NON CORE | Summary: Negative regulation of Hedgehog signaling. Reason: Cul1-based SCF(Slimb) processes Ci; for the shared cullin scaffold this pathway output is non-core (it is accepted as a defining role for Slimb). Supporting Evidence: PMID:12231629 In cells anterior to the MF, Ci proteolytic processing promoted by PKA requires the activity of the Nedd8-modified Cul1-based SCF(Slimb) complex. |
| GO:0045879 negative regulation of smoothened signaling pathway | IMP PMID:26120032 Deubiquitination of Ci/Gli by Usp7/HAUSP Regulates Hedgehog ... | KEEP AS NON CORE | Summary: Negative regulation of Hedgehog signaling. Reason: Cul1-based SCF(Slimb) processes Ci; for the shared cullin scaffold this pathway output is non-core (it is accepted as a defining role for Slimb). Supporting Evidence: PMID:26120032 inhibiting Ci ubiquitination and degradation mediated by both Slimb-Cul1 and Hib-Cul3 E3 ligases |
| GO:0046627 negative regulation of insulin receptor signaling pathway | IGI PMID:24068890 A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K... | KEEP AS NON CORE | Summary: Negative regulation of insulin receptor signaling. Reason: SCF-Cul1 inactivates InR/PI3K/TOR during dendrite pruning; context-specific output. Supporting Evidence: PMID:24068890 the Cullin1-based E3 ligase facilitates ddaC dendrite pruning primarily through inactivation of the InR/PI3K/TOR pathway |
| GO:0051898 negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction | IGI PMID:24068890 A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K... | KEEP AS NON CORE | Summary: Negative regulation of PI3K/Akt signaling. Reason: SCF-Cul1 promotes Akt ubiquitination via Slimb during pruning; context-specific. Supporting Evidence: PMID:24068890 the F-box protein Slimb forms a complex with Akt, an activator of the InR/PI3K/TOR pathway, and promotes Akt ubiquitination |
| GO:0061060 negative regulation of peptidoglycan recognition protein signaling pathway | IMP PMID:12401167 A ubiquitin-proteasome pathway represses the Drosophila immu... | KEEP AS NON CORE | Summary: Negative regulation of PGRP (IMD) signaling. Reason: Cul1 mutations derepress Diptericin; non-core. Supporting Evidence: PMID:12401167 mutations affecting either the Drosophila SCF components, Slimb and dCullin1, or the proteasome also induce Diptericin expression |
| GO:0070979 protein K11-linked ubiquitination | IDA PMID:23747190 Ter94 ATPase complex targets k11-linked ubiquitinated ci to ... | ACCEPT | Summary: K11-linked ubiquitination of Ci. Reason: Cul1-Slimb E3 adds K11-linked chains to Ci; direct activity of the assembled ligase. Supporting Evidence: PMID:23747190 We demonstrate that Cul1-Slimb-based E3 ligase, but not Cul3-Rdx-based E3 ligase, modifies Ci by efficient addition of K11-linked ubiquitin chains. |
| GO:0090090 negative regulation of canonical Wnt signaling pathway | IMP PMID:21628596 Drosophila homeodomain-interacting protein kinase inhibits t... | KEEP AS NON CORE | Summary: Negative regulation of canonical Wnt signaling. Reason: Cul1-based SCF(Slimb) destroys Armadillo; non-core output for the shared scaffold. Supporting Evidence: PMID:21628596 Hipk acts to stabilize Armadillo by impeding the function of the E3 ubiquitin ligase Skp1-Cul1-F-box (SCF)(Slimb), thereby inhibiting Armadillo ubiquitination and subsequent degradation. |
| GO:0090090 negative regulation of canonical Wnt signaling pathway | IMP PMID:22359584 Defining components of the Γ-catenin destruction complex and... | KEEP AS NON CORE | Summary: Negative regulation of canonical Wnt signaling. Reason: Cul1-based SCF(Slimb) destroys Armadillo; non-core output for the shared scaffold. Supporting Evidence: PMID:22359584 Using RNAi in Drosophila S2 cells to examine which SCF components are essential for Armadillo destruction |
| GO:0160072 ubiquitin ligase complex scaffold activity | IBA GO_REF:0000033 | ACCEPT | Summary: Ubiquitin ligase complex scaffold activity. Reason: Defining molecular function of cullins: Cul1 binds SkpA/F-box and Roc1a modules and positions substrate and E2. |
| GO:0160072 ubiquitin ligase complex scaffold activity | IPI PMID:11500045 Occurrence of a putative SCF ubiquitin ligase complex in Dro... | ACCEPT | Summary: Ubiquitin ligase complex scaffold activity. Reason: Defining molecular function of cullins: Cul1 binds SkpA/F-box and Roc1a modules and positions substrate and E2. Supporting Evidence: PMID:11500045 We show that putative Drosophila SCF core subunits dSkpA and dRbx1 both interact directly with dCu11 and the F-box protein Slmb. |
| GO:0160072 ubiquitin ligase complex scaffold activity | IPI PMID:17925225 Regulation of Ci-SCFSlimb binding, Ci proteolysis, and hedge... | ACCEPT | Summary: Ubiquitin ligase complex scaffold activity. Reason: Defining molecular function of cullins: Cul1 binds SkpA/F-box and Roc1a modules and positions substrate and E2. Supporting Evidence: PMID:17925225 When Hh is absent, phosphorylation of Ci/Gli triggers binding to SCF ubiquitin ligase complexes and consequent proteolysis. |
| GO:0160072 ubiquitin ligase complex scaffold activity | IPI PMID:18698375 Identifying determinants of cullin binding specificity among... | ACCEPT | Summary: Ubiquitin ligase complex scaffold activity. Reason: Defining molecular function of cullins: Cul1 binds SkpA/F-box and Roc1a modules and positions substrate and E2. Supporting Evidence: PMID:18698375 Cullin binding specificity is strongly influenced by the Roc NH(2)-terminal domain, which forms an inter-molecular beta sheet with the Cullin |
| GO:1902532 negative regulation of intracellular signal transduction | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Negative regulation of intracellular signal transduction (ARBA). Reason: Very broad grouping of the Hh, Wnt and InR pathway outputs; valid but uninformative. Non-core. |
| GO:1904801 positive regulation of neuron remodeling | IMP PMID:24068890 A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K... | KEEP AS NON CORE | Summary: Positive regulation of neuron remodeling. Reason: Cul1 is required for ddaC dendrite and MB axon pruning; developmental output. Supporting Evidence: PMID:24068890 Here, we identified a conserved SCF E3 ubiquitin ligase that plays a critical role in pruning of both ddaC dendrites and mushroom body Ξ³ axons. |
| GO:1905088 positive regulation of synaptonemal complex assembly | IMP PMID:33382409 SCF-Fbxo42 promotes synaptonemal complex assembly by downreg... | KEEP AS NON CORE | Summary: Positive regulation of synaptonemal complex assembly. Reason: SCF core is needed for synaptonemal complex assembly in female meiosis; non-core. Supporting Evidence: PMID:33382409 We found that the ubiquitin ligase complex SCF is important for assembly and maintenance of the synaptonemal complex in Drosophila female meiosis. |
Loading supporting contentβ¦
Download this section (compressed HTML)Q: How is Cul1 neddylation/deneddylation (CSN) cycling coordinated with F-box exchange (Cand1) to switch between SCF(Slimb), SCF(Ago) and testis SCF(Nutcracker) in vivo?
Experiment: Rescue Cul1 null clones with wild-type or Nedd8-site mutant Cul1 and measure Ci-75/Ci-155 ratios, Arm and Cyclin E levels in wing and eye discs.
Hypothesis: Neddylation-defective Cul1 (K->R at the conserved Nedd8 site) cannot support Ci processing or Cyclin E degradation in vivo.
Loading supporting contentβ¦
Download this section (compressed HTML)Loading supporting contentβ¦
Download this section (compressed HTML)Loading supporting contentβ¦
Download this section (compressed HTML)