id: Q9VVS1
gene_symbol: Dic4
taxon:
  id: NCBITaxon:7227
  label: Drosophila melanogaster
status: COMPLETE
description: 'The ProtNLM2 API snapshot retrieved 2026-09-08 contains no GO-term predictions for Q9VVS1.
  Mitochondrion (GO:0005739) is a supported omission candidate because the primary Dic4 study reports
  mitochondrial localization of the expressed Dic proteins, and the current exact accession is the native
  302-residue Dic4 product. A specific transport-activity omission is not established: reconstituted Dic4
  showed no detectable transport in the published dicarboxylate-carrier assays, while the accessible abstract
  does not establish that thiamine pyrophosphate was tested. The separate FUNCTION prediction of mitochondrial
  thiamine pyrophosphate uptake therefore remains UNC. This completed record assesses the absence of GO
  output; predictions is empty because no GO or EC prediction was emitted. The narrative judgment remains
  in the linked function review and is not a score assigned to the omission.'
source_documents:
- genes/DROME/Dic4/Dic4-protnlm-source.json
- projects/PROTNLM_EVALUATION/fly-benchmark/manifest.json
- genes/DROME/Dic4/Dic4-protnlm-function-review.md
- genes/DROME/Dic4/Dic4-uniprot.txt
- publications/PMID_21130726.md
- genes/DROME/Dic4/Dic4-hypotheses/fly41-thiamine-pyrophosphate-transport/openscientist.md
references:
- id: file:DROME/Dic4/Dic4-uniprot.txt
  title: Dic4 UniProt identity and localization record
  findings:
  - statement: The exact accession encodes a 302-residue product.
    supporting_text: ID   Q9VVS1_DROME            Unreviewed;       302 AA.
  - statement: The mitochondrial localization is represented by a direct-assay FlyBase annotation.
    supporting_text: DR   GO; GO:0005739; C:mitochondrion; IDA:FlyBase.
- id: PMID:21130726
  title: 'A novel subfamily of mitochondrial dicarboxylate carriers from Drosophila melanogaster: biochemical
    and computational studies.'
  findings:
  - statement: The primary study reports mitochondrial localization for all expressed Dic proteins, including
      Dic4.
    supporting_text: 'All expressed proteins are localized in

      mitochondria.'
  - statement: Dic4 lacked detectable transport in the reported assay panel; this is not an exclusion
      of every untested substrate.
    supporting_text: 'No

      transport activity was observed for DmDic4p.'
  full_text_unavailable: true
- id: file:DROME/Dic4/Dic4-hypotheses/fly41-thiamine-pyrophosphate-transport/openscientist.md
  title: OpenScientist audit of Dic4 mitochondrial thiamine pyrophosphate transport
  findings:
  - statement: OpenScientist found no positive support for ThPP transport by Dic4 and traced that specific
      activity to the characterized Tpc1 carrier.
    supporting_text: The most important caveat is that ThPP was likely never directly assayed against Dic4,
      so it is unmeasured rather than formally excluded — but no positive evidence supports the assignment,
      and the function is already correctly located on Tpc1.
predictions: []