Fbxo42 is the substrate-recognizing F-box subunit of an SCF ubiquitin ligase. In Drosophila oocytes, SCF-Fbxo42 downregulates the PP2A-B56 phosphatase subunit and promotes assembly and maintenance of the synaptonemal complex. Its F-box and kelch-like architecture support substrate recognition and complex assembly rather than intrinsic ubiquitin transfer.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0019005 SCF ubiquitin ligase complex | IBA GO_REF:0000033 | ACCEPT | Summary: SCF membership is supported by direct fly biochemistry. Reason: PMID:33382409 identifies Fbxo42 as an SCF substrate-recognition subunit; the IBA is concordant with direct target evidence and is not circular. Supporting Evidence: file:DROME/Fbxo42/Fbxo42-uniprot.txt DR InterPro; IPR036047; F-box-like_dom_sf. file:DROME/Fbxo42/Fbxo42-uniprot.txt DR InterPro; IPR001810; F-box_dom. file:DROME/Fbxo42/Fbxo42-uniprot.txt FT DOMAIN 21..70 FT /note="F-box" FT /evidence="ECO:0000259|PROSITE:PS50181" PMID:33382409 Our biochemical and genetic evidence suggests that Fbxo42 promotes synaptonemal complex assembly by negatively controlling the level of the protein phosphatase PP2A-B56. |
| GO:0019005 SCF ubiquitin ligase complex | IDA PMID:33382409 SCF-Fbxo42 promotes synaptonemal complex assembly by downreg... | ACCEPT | Summary: Fbxo42 is an SCF-complex component. Reason: Biochemical interaction and genetic analyses in PMID:33382409 establish the Fbxo42 SCF complex in Drosophila ovaries. Supporting Evidence: file:DROME/Fbxo42/Fbxo42-uniprot.txt DR InterPro; IPR036047; F-box-like_dom_sf. file:DROME/Fbxo42/Fbxo42-uniprot.txt DR InterPro; IPR001810; F-box_dom. file:DROME/Fbxo42/Fbxo42-uniprot.txt FT DOMAIN 21..70 FT /note="F-box" FT /evidence="ECO:0000259|PROSITE:PS50181" PMID:33382409 Our biochemical and genetic evidence suggests that Fbxo42 promotes synaptonemal complex assembly by negatively controlling the level of the protein phosphatase PP2A-B56. |
| GO:0019005 SCF ubiquitin ligase complex | ISM GO_REF:0000050 | ACCEPT | Summary: F-box architecture is consistent with SCF assembly. Reason: The independent F-box domain at residues 21-70 and direct complex evidence in PMID:33382409 support the computational annotation. Supporting Evidence: file:DROME/Fbxo42/Fbxo42-uniprot.txt DR InterPro; IPR036047; F-box-like_dom_sf. file:DROME/Fbxo42/Fbxo42-uniprot.txt DR InterPro; IPR001810; F-box_dom. file:DROME/Fbxo42/Fbxo42-uniprot.txt FT DOMAIN 21..70 FT /note="F-box" FT /evidence="ECO:0000259|PROSITE:PS50181" PMID:33382409 Our biochemical and genetic evidence suggests that Fbxo42 promotes synaptonemal complex assembly by negatively controlling the level of the protein phosphatase PP2A-B56. |
| GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process | ISM GO_REF:0000050 | ACCEPT | Summary: SCF-dependent substrate downregulation is a core role. Reason: PMID:33382409 shows that SCF-Fbxo42 downregulates PP2A-B56 in the pathway promoting synaptonemal-complex assembly. The protein is the substrate adaptor rather than the catalytic ubiquitin-transfer enzyme. Supporting Evidence: file:DROME/Fbxo42/Fbxo42-uniprot.txt DR InterPro; IPR036047; F-box-like_dom_sf. file:DROME/Fbxo42/Fbxo42-uniprot.txt DR InterPro; IPR001810; F-box_dom. file:DROME/Fbxo42/Fbxo42-uniprot.txt FT DOMAIN 21..70 FT /note="F-box" FT /evidence="ECO:0000259|PROSITE:PS50181" PMID:33382409 Our biochemical and genetic evidence suggests that Fbxo42 promotes synaptonemal complex assembly by negatively controlling the level of the protein phosphatase PP2A-B56. |
| GO:1905088 positive regulation of synaptonemal complex assembly | IMP PMID:33382409 SCF-Fbxo42 promotes synaptonemal complex assembly by downreg... | ACCEPT | Summary: Synaptonemal-complex assembly is a direct physiological function. Reason: The full text of PMID:33382409 documents impaired assembly and maintenance after Fbxo42 depletion and connects this phenotype to PP2A-B56 regulation. Supporting Evidence: file:DROME/Fbxo42/Fbxo42-uniprot.txt DR InterPro; IPR036047; F-box-like_dom_sf. file:DROME/Fbxo42/Fbxo42-uniprot.txt DR InterPro; IPR001810; F-box_dom. file:DROME/Fbxo42/Fbxo42-uniprot.txt FT DOMAIN 21..70 FT /note="F-box" FT /evidence="ECO:0000259|PROSITE:PS50181" PMID:33382409 Our biochemical and genetic evidence suggests that Fbxo42 promotes synaptonemal complex assembly by negatively controlling the level of the protein phosphatase PP2A-B56. |
| GO:1990756 ubiquitin-like ligase-substrate adaptor activity | IBA GO_REF:0000033 | ACCEPT | Summary: Substrate-adaptor activity is the appropriate molecular function. Reason: F-box-mediated SCF incorporation and substrate-recognition evidence support the IBA; they do not establish intrinsic ubiquitin ligase catalysis by Fbxo42 alone. Supporting Evidence: file:DROME/Fbxo42/Fbxo42-uniprot.txt DR InterPro; IPR036047; F-box-like_dom_sf. file:DROME/Fbxo42/Fbxo42-uniprot.txt DR InterPro; IPR001810; F-box_dom. file:DROME/Fbxo42/Fbxo42-uniprot.txt FT DOMAIN 21..70 FT /note="F-box" FT /evidence="ECO:0000259|PROSITE:PS50181" PMID:33382409 Our biochemical and genetic evidence suggests that Fbxo42 promotes synaptonemal complex assembly by negatively controlling the level of the protein phosphatase PP2A-B56. |
| GO:1990756 ubiquitin-like ligase-substrate adaptor activity | IPI PMID:33382409 SCF-Fbxo42 promotes synaptonemal complex assembly by downreg... | ACCEPT | Summary: Direct fly evidence supports substrate-recognition activity. Reason: PMID:33382409 identifies Fbxo42 as a substrate-recognizing SCF subunit and PP2A-B56 as the regulated target. This is more informative than generic protein binding. Supporting Evidence: file:DROME/Fbxo42/Fbxo42-uniprot.txt DR InterPro; IPR036047; F-box-like_dom_sf. file:DROME/Fbxo42/Fbxo42-uniprot.txt DR InterPro; IPR001810; F-box_dom. file:DROME/Fbxo42/Fbxo42-uniprot.txt FT DOMAIN 21..70 FT /note="F-box" FT /evidence="ECO:0000259|PROSITE:PS50181" PMID:33382409 Our biochemical and genetic evidence suggests that Fbxo42 promotes synaptonemal complex assembly by negatively controlling the level of the protein phosphatase PP2A-B56. |
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