GatC (CG33649) encodes the small C subunit of the mitochondrial glutamyl-tRNA(Gln) amidotransferase (GatCAB), which converts glutamate mischarged onto mitochondrial tRNA(Gln) into glutamine, providing Gln-tRNA(Gln) for mitochondrial translation. GatC has no catalytic activity of its own; it wraps around the interface of the catalytic GatA and GatB subunits and is needed for assembly and stability of the heterotrimer. Function is inferred from orthologs; GatC has not been studied directly in flies.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0003674 molecular_function | ND GO_REF:0000015 | ACCEPT | Summary: Root molecular function (no data). Reason: ACCEPT under the ND acceptance rule (projects/NO_DATA.md). The evidence this review cites for the molecular function is inferred (from orthologs, family membership or sequence features) or comes from other species; none of it is literature on this gene product in this species. This was judged from the review's own content during the NO_DATA re-action pass, not from a new literature search. Earlier action: KEEP_AS_NON_CORE. The gene's own literature-search record finds no study of this gene product in this species that identifies a molecular function. Supporting Evidence: file:DROME/GatC/GatC-deep-research-falcon.md Strong support for the function of the GatC family, but no structure or binding experiment was performed with fly Q86BL4. |
| GO:0005739 mitochondrion | IBA GO_REF:0000033 | ACCEPT | Summary: Mitochondrion. Reason: GatCAB transamidates mitochondrial Glu-tRNA(Gln); all three fly subunits belong to the mitochondrial Gat subfamilies. Supporting Evidence: PMID:26761199 converting Glu to Gln via a heterotrimeric Glu-tRNAGln amidotransferase (Gat) |
| GO:0005739 mitochondrion | IEA GO_REF:0000120 | ACCEPT | Summary: Mitochondrion. Reason: GatCAB transamidates mitochondrial Glu-tRNA(Gln); all three fly subunits belong to the mitochondrial Gat subfamilies. Supporting Evidence: PMID:26761199 converting Glu to Gln via a heterotrimeric Glu-tRNAGln amidotransferase (Gat) |
| GO:0005739 mitochondrion | ISS GO_REF:0000024 | ACCEPT | Summary: Mitochondrion. Reason: GatCAB transamidates mitochondrial Glu-tRNA(Gln); all three fly subunits belong to the mitochondrial Gat subfamilies. Supporting Evidence: PMID:26761199 converting Glu to Gln via a heterotrimeric Glu-tRNAGln amidotransferase (Gat) |
| GO:0005759 mitochondrial matrix | IC GO_REF:0000111 | ACCEPT | Summary: Mitochondrial matrix. Reason: The transamidosome acts on mitochondrially encoded tRNA(Gln) in the matrix. Supporting Evidence: PMID:26761199 converting Glu to Gln via a heterotrimeric Glu-tRNAGln amidotransferase (Gat) |
| GO:0006450 regulation of translational fidelity | IEA GO_REF:0000002 | MARK AS OVER ANNOTATED | Summary: Regulation of translational fidelity (InterPro). Reason: GatCAB is the biosynthetic route to mitochondrial Gln-tRNA(Gln), not a regulator of the fidelity of decoding; its contribution to correct translation is captured by conversion of glutamyl-tRNA to glutaminyl-tRNA and mitochondrial translation. Supporting Evidence: file:DROME/GatC/GatC-uniprot.txt This subunit probably forms a belt |
| GO:0030956 glutamyl-tRNA(Gln) amidotransferase complex | IBA GO_REF:0000033 | ACCEPT | Summary: Subunit of the glutamyl-tRNA(Gln) amidotransferase (GatCAB) complex. Reason: Flies encode single GatA, GatB and GatC orthologs, which together form the heterotrimeric mitochondrial amidotransferase. Supporting Evidence: PMID:26761199 CG5463 and CG33649 genes – we propose to name the latter two GatB and GatC, respectively |
| GO:0030956 glutamyl-tRNA(Gln) amidotransferase complex | IEA GO_REF:0000104 | ACCEPT | Summary: Subunit of the glutamyl-tRNA(Gln) amidotransferase (GatCAB) complex. Reason: Flies encode single GatA, GatB and GatC orthologs, which together form the heterotrimeric mitochondrial amidotransferase. Supporting Evidence: PMID:26761199 CG5463 and CG33649 genes – we propose to name the latter two GatB and GatC, respectively |
| GO:0030956 glutamyl-tRNA(Gln) amidotransferase complex | ISS GO_REF:0000024 | ACCEPT | Summary: Subunit of the glutamyl-tRNA(Gln) amidotransferase (GatCAB) complex. Reason: Flies encode single GatA, GatB and GatC orthologs, which together form the heterotrimeric mitochondrial amidotransferase. Supporting Evidence: PMID:26761199 CG5463 and CG33649 genes – we propose to name the latter two GatB and GatC, respectively |
| GO:0030956 glutamyl-tRNA(Gln) amidotransferase complex | ISS PMID:26761199 The aminoacyl-tRNA synthetases of Drosophila melanogaster. | ACCEPT | Summary: Subunit of the glutamyl-tRNA(Gln) amidotransferase (GatCAB) complex. Reason: Flies encode single GatA, GatB and GatC orthologs, which together form the heterotrimeric mitochondrial amidotransferase. Supporting Evidence: PMID:26761199 CG5463 and CG33649 genes – we propose to name the latter two GatB and GatC, respectively |
| GO:0032543 mitochondrial translation | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: Mitochondrial translation. Reason: GatCAB supplies Gln-tRNA(Gln) for mitochondrial protein synthesis (there is no mitochondrial GlnRS), so the involvement is real, but the specific step is captured better by conversion of glutamyl-tRNA to glutaminyl-tRNA. Supporting Evidence: PMID:26761199 converting Glu to Gln via a heterotrimeric Glu-tRNAGln amidotransferase (Gat) |
| GO:0032543 mitochondrial translation | IEA GO_REF:0000104 | KEEP AS NON CORE | Summary: Mitochondrial translation. Reason: GatCAB supplies Gln-tRNA(Gln) for mitochondrial protein synthesis (there is no mitochondrial GlnRS), so the involvement is real, but the specific step is captured better by conversion of glutamyl-tRNA to glutaminyl-tRNA. Supporting Evidence: PMID:26761199 converting Glu to Gln via a heterotrimeric Glu-tRNAGln amidotransferase (Gat) |
| GO:0032543 mitochondrial translation | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Mitochondrial translation. Reason: GatCAB supplies Gln-tRNA(Gln) for mitochondrial protein synthesis (there is no mitochondrial GlnRS), so the involvement is real, but the specific step is captured better by conversion of glutamyl-tRNA to glutaminyl-tRNA. Supporting Evidence: PMID:26761199 converting Glu to Gln via a heterotrimeric Glu-tRNAGln amidotransferase (Gat) |
| GO:0070681 obsolete glutaminyl-tRNAGln biosynthesis via transamidation | IBA GO_REF:0000033 | MODIFY | Summary: Obsolete glutaminyl-tRNAGln biosynthesis via transamidation. Reason: The term was obsoleted with no automatic replacement. The subunit takes part in transamidation of misacylated Glu-tRNA(Gln) to Gln-tRNA(Gln), which is described by GO:0043685 conversion of glutamyl-tRNA to glutaminyl-tRNA. Propagation Review Root cause: SOURCE STALE OR MISSING Proposed replacements: conversion of glutamyl-tRNA to glutaminyl-tRNA Supporting Evidence: PMID:26761199 converting Glu to Gln via a heterotrimeric Glu-tRNAGln amidotransferase (Gat) |
| GO:0070681 obsolete glutaminyl-tRNAGln biosynthesis via transamidation | IEA GO_REF:0000104 | MODIFY | Summary: Obsolete glutaminyl-tRNAGln biosynthesis via transamidation. Reason: The term was obsoleted with no automatic replacement. The subunit takes part in transamidation of misacylated Glu-tRNA(Gln) to Gln-tRNA(Gln), which is described by GO:0043685 conversion of glutamyl-tRNA to glutaminyl-tRNA. Propagation Review Root cause: SOURCE STALE OR MISSING Proposed replacements: conversion of glutamyl-tRNA to glutaminyl-tRNA Supporting Evidence: PMID:26761199 converting Glu to Gln via a heterotrimeric Glu-tRNAGln amidotransferase (Gat) |
| GO:0070681 obsolete glutaminyl-tRNAGln biosynthesis via transamidation | ISS PMID:26761199 The aminoacyl-tRNA synthetases of Drosophila melanogaster. | MODIFY | Summary: Obsolete glutaminyl-tRNAGln biosynthesis via transamidation. Reason: The term was obsoleted with no automatic replacement. The subunit takes part in transamidation of misacylated Glu-tRNA(Gln) to Gln-tRNA(Gln), which is described by GO:0043685 conversion of glutamyl-tRNA to glutaminyl-tRNA. Propagation Review Root cause: SOURCE STALE OR MISSING Proposed replacements: conversion of glutamyl-tRNA to glutaminyl-tRNA Supporting Evidence: PMID:26761199 converting Glu to Gln via a heterotrimeric Glu-tRNAGln amidotransferase (Gat) |
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Download this section (compressed HTML)Q: Is GatC required for GatA/GatB protein stability in flies, as reported for the human complex?
Experiment: Immunoblot GatA and GatB and assay mt-tRNA(Gln) charging in GatC mutant or RNAi flies.
Hypothesis: GatC loss destabilizes GatA and GatB and blocks mitochondrial Gln-tRNA(Gln) formation.
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