Ing5

UniProt ID: Q9VJY8
Organism: Drosophila melanogaster
Review Status: COMPLETE
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Gene Description

Ing5 is a Drosophila member of the inhibitor of growth (ING) family, with a C-terminal PHD finger of the type that recognizes histone H3 trimethylated at lysine 4. It is a subunit of the Enok (MOZ/MORF-type) histone acetyltransferase complex with the catalytic subunit Enok, the scaffold Br140 and Eaf6, and is required for the complex's acetylation of histone H3 lysine 23 in vivo. Through the complex it contributes to promoting the G1/S transition by restraining PCNA unloading.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0005634 nucleus
EXP
PMID:27198229
The Enok acetyltransferase complex interacts with Elg1 and n... 		ACCEPT
Summary: Ing5 is detected in nuclear fractions.
Reason: Correct; consistent across Enok complex subunits.
Supporting Evidence:
file:DROME/Ing5/Ing5-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
GO:0005634 nucleus
IBA
GO_REF:0000033 		ACCEPT
Summary: Ing5 is nuclear.
Reason: Correct; consistent across Enok complex subunits.
Supporting Evidence:
file:DROME/Ing5/Ing5-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
GO:0005634 nucleus
IEA
GO_REF:0000120 		ACCEPT
Summary: Ing5 is nuclear.
Reason: Correct; consistent across Enok complex subunits.
Supporting Evidence:
file:DROME/Ing5/Ing5-uniprot.txt
SUBCELLULAR LOCATION: Nucleus
GO:0005694 chromosome
IEA
GO_REF:0000044 		ACCEPT
Summary: Chromosome localization by UniProt mapping and ISS from human ING5 (UniProtKB:Q8WYH8).
Reason: ING proteins bind chromatin via their PHD finger; chromosome is as precise as these sources allow.
Supporting Evidence:
file:DROME/Ing5/Ing5-uniprot.txt
Chromosome
GO:0005694 chromosome
ISS
GO_REF:0000024 		ACCEPT
Summary: Chromosome localization by UniProt mapping and ISS from human ING5 (UniProtKB:Q8WYH8).
Reason: ING proteins bind chromatin via their PHD finger; chromosome is as precise as these sources allow.
Supporting Evidence:
file:DROME/Ing5/Ing5-uniprot.txt
Chromosome
GO:0006271 DNA strand elongation involved in DNA replication
IDA
PMID:27198229
The Enok acetyltransferase complex interacts with Elg1 and n... 		MODIFY
Summary: The Enok complex promotes the G1/S transition by restraining Elg1-mediated PCNA unloading.
Reason: The study shows a G1/S block and reduced chromatin-bound PCNA after Enok depletion and an Enok complex-Elg1 interaction; it does not measure DNA strand elongation. Replace with GO:0000082 G1/S transition of mitotic cell cycle. Shared complex process treated identically across the four Enok complex subunits.
Supporting Evidence:
PMID:27198229
Depletion of Enok resulted in an Elg1-dependent block at the G1/S transition and reduced chromatin-bound PCNA levels.
GO:0006325 chromatin organization
IBA
GO_REF:0000033 		KEEP AS NON CORE
Summary: IBA for chromatin organization (PTN008942935; donors include FB:FBgn0030945, UniProtKB:Q8WYH8 ING5, Q9UNL4 ING4, yeast proteins).
Reason: Plausible via the Enok complex's histone acetylation, but general and not Ing5's own activity.
GO:0006355 regulation of DNA-templated transcription
IBA
GO_REF:0000033 		KEEP AS NON CORE
Summary: IBA for regulation of transcription (PTN000035903; donors include UniProtKB:Q8WYH8 ING5, Q9H160 ING2, Q9UNL4 ING4).
Reason: Plausible for an ING subunit of a HAT complex; no Ing5-specific Drosophila evidence on direction or targets.
GO:0010698 acetyltransferase activator activity
IDA
PMID:27198229
The Enok acetyltransferase complex interacts with Elg1 and n... 		ACCEPT
Summary: Ing5 is required for the Enok complex's H3K23 acetyltransferase activity.
Reason: Depletion of Ing5 reduces H3K23ac; shared term treated identically across Br140, Ing5 and Eaf6.
Supporting Evidence:
PMID:27198229
Depletion of any of the four subunits led to reductions in the H3K23ac levels without affecting the H3K14ac levels
PMID:27198229
we concluded that all four subunits are important for full HAT activity of the Enok complex
GO:0032991 protein-containing complex
IEA
GO_REF:0000117 		MODIFY
Summary: ARBA prediction of protein-containing complex.
Reason: Correct but uninformative; replace with GO:0070776 MOZ/MORF histone acetyltransferase complex, already annotated.
GO:0070776 MOZ/MORF histone acetyltransferase complex
IBA
GO_REF:0000033 		ACCEPT
Summary: IBA for MOZ/MORF complex (PTN000817813; donors FB:FBgn0032516 Ing5 itself and UniProtKB:Q8WYH8 ING5).
Reason: Consistent with direct biochemical evidence in flies.
GO:0070776 MOZ/MORF histone acetyltransferase complex
IDA
PMID:27198229
The Enok acetyltransferase complex interacts with Elg1 and n... 		ACCEPT
Summary: Subunit of the Enok (KAT6, MOZ/MORF-type) acetyltransferase complex.
Reason: Core complex. Enok, Br140, Ing5 and Eaf6 co-purify reciprocally and each is required for the complex's H3K23 acetylation; shared complex term treated identically across the four subunits.
Supporting Evidence:
PMID:27198229
MudPIT analysis of Flag affinity purifications of Flag-HA-tagged Enok, Br140, Eaf6, and Ing5 showed copurification of these four components.
GO:0070776 MOZ/MORF histone acetyltransferase complex
IPI
PMID:27198229
The Enok acetyltransferase complex interacts with Elg1 and n... 		ACCEPT
Summary: Subunit of the Enok (KAT6, MOZ/MORF-type) acetyltransferase complex.
Reason: Core complex. Enok, Br140, Ing5 and Eaf6 co-purify reciprocally and each is required for the complex's H3K23 acetylation; shared complex term treated identically across the four subunits.
Supporting Evidence:
PMID:27198229
MudPIT analysis of Flag affinity purifications of Flag-HA-tagged Enok, Br140, Eaf6, and Ing5 showed copurification of these four components.
GO:0140002 histone H3K4me3 reader activity
IBA
GO_REF:0000033 		ACCEPT
Summary: IBA for H3K4me3 reader activity (PTN008942935; donors include human ING proteins Q8WYH8, Q9H160, Q9NXR8, Q9UK53, yeast and Arabidopsis ING proteins).
Reason: The ING PHD finger that reads H3K4me3 is conserved across the family, and Ing5 is inside the clade; consistent with ING5 function in the human MOZ/MORF and HBO1 complexes. Accepted as a phylogenetic inference; H3K4me3 binding has not been measured for fly Ing5, so it is not used as the core molecular function.

Core Functions

ING-family subunit of the Enok complex required for the complex's H3K23 acetylation activity and for Enok association with chromatin. By family inference (not yet measured in flies) its C-terminal PHD finger is expected to recognize H3K4me3.

Molecular Function:
acetyltransferase activator activity
Directly Involved In:
G1/S transition of mitotic cell cycle
Cellular Locations:
nucleus
In Complex:
MOZ/MORF histone acetyltransferase complex
Supporting Evidence:
  • PMID:27198229
    Depletion of any of the four subunits led to reductions in the H3K23ac levels without affecting the H3K14ac levels
  • PMID:27198229
    we concluded that all four subunits are important for full HAT activity of the Enok complex
  • file:DROME/Ing5/Ing5-deep-research-falcon.md
    Its experimentally demonstrated contribution is to promote Enok association with nuclear chromatin and appropriate histone H3 lysine-23 acetylation

References

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Suggested Questions for Experts

Q: Does Drosophila Ing5 also join the HBO1/Chameau complex, as human ING5 does, and how is it partitioned between complexes?

Deep Research

Falcon

(Ing5-deep-research-falcon.md)

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FlyBase

(Ing5-deep-research-flybase.md)
FlyBase gene description for Ing5 (DROME) FlyBase

FlyBase gene description for Ing5 (DROME)

Inhibitor of growth family member 5 (Ing5) encodes a histone acetyltransferase that forms part of the Enok complex, which interacts with the product of elg1 to promote the G1/S transition via proliferating cell nuclear antigen (PCNA) unloading.

📚 Additional Documentation

Notes

(Ing5-notes.md)

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