Serine/threonine kinase STK11 that activates AMPK-family kinases and regulates polarity, growth, and energy stress responses.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0004674 protein serine/threonine kinase activity | IBA GO_REF:0000033 | ACCEPT | Summary: LKB1 is a serine/threonine protein kinase. Reason: UniProt catalytic activity indicates serine/threonine kinase function. Supporting Evidence: file:genes/DROME/Lkb1/Lkb1-uniprot.txt L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+) file:genes/DROME/Lkb1/Lkb1-deep-research-falcon.md RecName: Full=Serine/threonine-protein kinase STK11 |
| GO:0005737 cytoplasm | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: LKB1 localizes to the cytoplasm. Reason: UniProt lists cytoplasmic localization. Supporting Evidence: file:genes/DROME/Lkb1/Lkb1-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. |
| GO:0035556 intracellular signal transduction | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: LKB1 participates in intracellular signal transduction via AMPK-related kinases. Reason: Regulation of AMPK-family kinases places LKB1 in intracellular signaling pathways. Supporting Evidence: PMID:25996931 LKB1 plays important roles in governing energy homeostasis by regulating AMP-activated protein kinase (AMPK) and other AMPK-related kinases |
| GO:0000166 nucleotide binding | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: LKB1 binds nucleotides as part of its kinase reaction. Reason: The catalytic reaction uses ATP, indicating nucleotide binding. Supporting Evidence: file:genes/DROME/Lkb1/Lkb1-uniprot.txt L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+) |
| GO:0001558 regulation of cell growth | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: LKB1 regulates organ growth through apoptosis signaling. Reason: LKB1 negatively regulates organ growth via apoptosis. Supporting Evidence: PMID:16273080 Drosophila LKB1 negatively regulates organ growth by caspase-dependent apoptosis |
| GO:0004672 protein kinase activity | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: LKB1 has protein kinase activity. Reason: Catalytic activity confirms protein kinase function. Supporting Evidence: file:genes/DROME/Lkb1/Lkb1-uniprot.txt L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+) |
| GO:0004674 protein serine/threonine kinase activity | IEA GO_REF:0000120 | ACCEPT | Summary: LKB1 is a serine/threonine protein kinase. Reason: UniProt catalytic activity indicates serine/threonine kinase function. Supporting Evidence: file:genes/DROME/Lkb1/Lkb1-uniprot.txt L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+) |
| GO:0005524 ATP binding | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: LKB1 uses ATP in its catalytic reaction. Reason: The kinase reaction explicitly consumes ATP. Supporting Evidence: file:genes/DROME/Lkb1/Lkb1-uniprot.txt L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+) |
| GO:0005634 nucleus | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: LKB1 can localize to the nucleus per UniProt annotation. Reason: UniProt lists nucleus as a subcellular location. Supporting Evidence: file:genes/DROME/Lkb1/Lkb1-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. Nucleus |
| GO:0005737 cytoplasm | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: LKB1 localizes to the cytoplasm. Reason: UniProt lists cytoplasmic localization. Supporting Evidence: file:genes/DROME/Lkb1/Lkb1-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. |
| GO:0006915 apoptotic process | IEA GO_REF:0000043 | MODIFY | Summary: LKB1 regulates apoptosis rather than being a core apoptotic machinery component. Reason: LKB1 induces apoptosis via JNK signaling; use positive regulation of apoptotic process. Proposed replacements: positive regulation of apoptotic process Supporting Evidence: PMID:16273080 Drosophila LKB1 negatively regulates organ growth by caspase-dependent apoptosis |
| GO:0006974 DNA damage response | IEA GO_REF:0000043 | UNDECIDED | Summary: DNA damage response is not supported by the accessible sources. Reason: No direct DNA damage response evidence was found in the available abstracts. Supporting Evidence: PMID:17507418 mutations disrupt spindle formation, resulting in frequent polyploid cells in larval brains |
| GO:0016301 kinase activity | IEA GO_REF:0000043 | KEEP AS NON CORE | Summary: LKB1 has kinase activity. Reason: Catalytic activity confirms kinase function. Supporting Evidence: file:genes/DROME/Lkb1/Lkb1-uniprot.txt L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+) |
| GO:0016740 transferase activity | IEA GO_REF:0000043 | KEEP AS NON CORE | Summary: LKB1 is a transferase (kinase) enzyme. Reason: Kinases transfer phosphate groups from ATP. Supporting Evidence: file:genes/DROME/Lkb1/Lkb1-uniprot.txt L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+) |
| GO:0030010 establishment of cell polarity | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: LKB1 is required for cell polarity establishment. Reason: Lkb1 is required for oocyte A-P polarity and epithelial polarity. Supporting Evidence: PMID:12540903 homologue, lkb1, is required for the early A-P polarity of the oocyte |
| GO:0030295 protein kinase activator activity | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: LKB1 activates AMPK and related kinases. Reason: LKB1 regulates AMPK and other AMPK-related kinases. Supporting Evidence: PMID:25996931 LKB1 plays important roles in governing energy homeostasis by regulating AMP-activated protein kinase (AMPK) and other AMPK-related kinases |
| GO:0042593 glucose homeostasis | IEA GO_REF:0000002 | UNDECIDED | Summary: Glucose homeostasis is not directly supported by the accessible evidence. Reason: Available sources focus on lipid metabolism and polarity rather than glucose homeostasis. Supporting Evidence: PMID:25996931 LKB1 mutants display decreased lipid storage and increased gene expression of brummer |
| GO:0046872 metal ion binding | IEA GO_REF:0000043 | KEEP AS NON CORE | Summary: LKB1 binds divalent metal ions required for catalysis. Reason: UniProt lists Mg2+ and Mn2+ as cofactors. Supporting Evidence: file:genes/DROME/Lkb1/Lkb1-uniprot.txt Name=Mg(2+) file:genes/DROME/Lkb1/Lkb1-uniprot.txt Name=Mn(2+) |
| GO:0008284 positive regulation of cell population proliferation | IMP PMID:36899949 Phosphorylation of LKB1 by PDK1 Inhibits Cell Proliferation ... | MARK AS OVER ANNOTATED | Summary: Positive regulation of cell proliferation is not supported for LKB1. Reason: LKB1 acts as a tumor suppressor and restricts growth; the cited study links LKB1 inhibition to increased growth. Supporting Evidence: PMID:16273080 Drosophila LKB1 negatively regulates organ growth by caspase-dependent apoptosis PMID:36899949 Phosphorylation of LKB1 by PDK1 Inhibits Cell Proliferation and Organ Growth by Decreased Activation of AMPK. |
| GO:0009898 cytoplasmic side of plasma membrane | IDA PMID:36899949 Phosphorylation of LKB1 by PDK1 Inhibits Cell Proliferation ... | KEEP AS NON CORE | Summary: LKB1 association with membranes is noted in the PDK1 interaction study. Reason: PDK1-mediated regulation of LKB1 involves membrane-associated interactions. Supporting Evidence: PMID:36899949 phosphorylation of LKB1 by PDK1 results in an inhibition of LKB1, decreased activation of AMPK and enhanced cell growth |
| GO:0043539 protein serine/threonine kinase activator activity | IDA PMID:36899949 Phosphorylation of LKB1 by PDK1 Inhibits Cell Proliferation ... | KEEP AS NON CORE | Summary: LKB1 activates serine/threonine kinases such as AMPK/SIKs. Reason: LKB1 regulates AMPK and other AMPK-related kinases. Supporting Evidence: PMID:25996931 LKB1 plays important roles in governing energy homeostasis by regulating AMP-activated protein kinase (AMPK) and other AMPK-related kinases PMID:36899949 Phosphorylation of LKB1 by PDK1 Inhibits Cell Proliferation and Organ Growth by Decreased Activation of AMPK. |
| GO:1904263 positive regulation of TORC1 signaling | IMP PMID:36899949 Phosphorylation of LKB1 by PDK1 Inhibits Cell Proliferation ... | MODIFY | Summary: Positive regulation of TORC1 signaling is inconsistent with LKB1βAMPK inhibition of mTOR. Reason: AMPK activation downstream of LKB1 inhibits mTOR; use negative regulation of TORC1 signaling instead. Proposed replacements: negative regulation of TORC1 signaling Supporting Evidence: PMID:36899949 Activation of AMPK by low energy supply and phosphorylation of LKB1 results in an inhibition of mTOR |
| GO:0007291 sperm individualization | IMP PMID:28767695 Drosophila LKB1 is required for the assembly of the polarize... | KEEP AS NON CORE | Summary: LKB1 is required for spermatid individualization. Reason: LKB1 is required for actin cone formation enabling spermatid individualization. Supporting Evidence: PMID:28767695 dLKB1 is required in the germline for the formation of the actin cone, the polarized structure that allows spermatid individualization |
| GO:0004674 protein serine/threonine kinase activity | IMP PMID:25996931 Feeding and Fasting Signals Converge on the LKB1-SIK3 Pathwa... | ACCEPT | Summary: LKB1 is a serine/threonine protein kinase. Reason: UniProt catalytic activity indicates serine/threonine kinase function. Supporting Evidence: file:genes/DROME/Lkb1/Lkb1-uniprot.txt L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+) PMID:25996931 Feeding and Fasting Signals Converge on the LKB1-SIK3 Pathway to Regulate Lipid Metabolism in Drosophila. |
| GO:0010897 negative regulation of triglyceride catabolic process | IMP PMID:25996931 Feeding and Fasting Signals Converge on the LKB1-SIK3 Pathwa... | KEEP AS NON CORE | Summary: LKB1 suppresses lipolysis and supports lipid storage. Reason: LKB1 mutants show decreased lipid storage and increased lipase expression. Supporting Evidence: PMID:25996931 LKB1 mutants display decreased lipid storage and increased gene expression of brummer |
| GO:0005515 protein binding | IPI PMID:29084199 Class III phosphatidylinositol-3-OH kinase controls epitheli... | UNDECIDED | Summary: Protein binding evidence is not explicit in the available abstract for this annotation. Reason: The endosomal regulation study does not explicitly document LKB1 binding partners in the abstract. Supporting Evidence: PMID:29084199 dysregulation of endosomally localized Liver Kinase B1 (LKB1, also known as STK11) |
| GO:0005768 endosome | IDA PMID:29084199 Class III phosphatidylinositol-3-OH kinase controls epitheli... | KEEP AS NON CORE | Summary: LKB1 localizes to endosomes in epithelial cells. Reason: Endosomal LKB1 localization is reported in epithelial integrity studies. Supporting Evidence: PMID:29084199 dysregulation of endosomally localized Liver Kinase B1 (LKB1, also known as STK11) |
| GO:0005938 cell cortex | IDA PMID:29084199 Class III phosphatidylinositol-3-OH kinase controls epitheli... | KEEP AS NON CORE | Summary: LKB1 accumulates at the cell cortex. Reason: LKB1 overexpression leads to cortical accumulation. Supporting Evidence: PMID:18054329 Lkb1, which accumulates in the cell cortex PMID:29084199 Class III phosphatidylinositol-3-OH kinase controls epithelial integrity through endosomal LKB1 regulation. |
| GO:0004674 protein serine/threonine kinase activity | ISS GO_REF:0000024 | ACCEPT | Summary: LKB1 is a serine/threonine protein kinase. Reason: UniProt catalytic activity indicates serine/threonine kinase function. Supporting Evidence: file:genes/DROME/Lkb1/Lkb1-uniprot.txt L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+) |
| GO:0005938 cell cortex | IDA PMID:18054329 The GC kinase Fray and Mo25 regulate Drosophila asymmetric d... | KEEP AS NON CORE | Summary: LKB1 accumulates at the cell cortex. Reason: LKB1 overexpression leads to cortical accumulation. Supporting Evidence: PMID:18054329 Lkb1, which accumulates in the cell cortex |
| GO:0072697 protein localization to cell cortex | IMP PMID:18054329 The GC kinase Fray and Mo25 regulate Drosophila asymmetric d... | KEEP AS NON CORE | Summary: LKB1 localizes to the cell cortex. Reason: LKB1 accumulates at the cell cortex in neuroblasts. Supporting Evidence: PMID:18054329 Lkb1, which accumulates in the cell cortex |
| GO:0072697 protein localization to cell cortex | IGI PMID:18054329 The GC kinase Fray and Mo25 regulate Drosophila asymmetric d... | KEEP AS NON CORE | Summary: LKB1 localizes to the cell cortex. Reason: LKB1 accumulates at the cell cortex in neuroblasts. Supporting Evidence: PMID:18054329 Lkb1, which accumulates in the cell cortex |
| GO:0045201 maintenance of neuroblast polarity | IDA PMID:22248825 Sgt1 acts via an LKB1/AMPK pathway to establish cortical pol... | KEEP AS NON CORE | Summary: LKB1 supports neuroblast cortical polarity. Reason: LKB1 mutants show apical cortical polarity defects in neuroblasts. Supporting Evidence: PMID:22248825 LKB1, or AMPKΞ± all show similar prophase apical cortical polarity defects |
| GO:0008340 determination of adult lifespan | IDA PMID:21281604 A gain-of-function screen identifies wdb and lkb1 as lifespa... | KEEP AS NON CORE | Summary: LKB1 overexpression extends lifespan in Drosophila. Reason: Gain-of-function lkb1 overexpression extends lifespan. Supporting Evidence: PMID:21281604 overexpression of the lkb1 gene encoding a serine/threonine kinase, reduced organ size and extended lifespan |
| GO:0007391 dorsal closure | IMP PMID:16273080 JNK pathway mediates apoptotic cell death induced by tumor s... | UNDECIDED | Summary: Dorsal closure is not explicitly supported by the available abstract evidence. Reason: The cited study discusses epithelial morphogenesis broadly, but not dorsal closure specifically. Supporting Evidence: PMID:16273080 Drosophila LKB1 negatively regulates organ growth by caspase-dependent apoptosis |
| GO:0043065 positive regulation of apoptotic process | IDA PMID:16273080 JNK pathway mediates apoptotic cell death induced by tumor s... | KEEP AS NON CORE | Summary: LKB1 promotes apoptosis via JNK signaling. Reason: LKB1 induces caspase-dependent apoptosis in Drosophila. Supporting Evidence: PMID:16273080 Drosophila LKB1 negatively regulates organ growth by caspase-dependent apoptosis |
| GO:0046328 regulation of JNK cascade | IDA PMID:16273080 JNK pathway mediates apoptotic cell death induced by tumor s... | KEEP AS NON CORE | Summary: LKB1 activates the JNK signaling pathway. Reason: The JNK pathway is activated by LKB1 and mediates LKB1-dependent apoptosis. Supporting Evidence: PMID:16273080 the JNK pathway was activated by LKB1 and mediated the LKB1-dependent apoptosis |
| GO:0030709 border follicle cell delamination | IMP PMID:18976916 PAR-1 kinase regulates epithelial detachment and directional... | UNDECIDED | Summary: Border follicle cell delamination is not supported for LKB1 in the cited abstract. Reason: The referenced study centers on PAR-1, not LKB1; direct evidence for LKB1 in border cell delamination is not accessible. Supporting Evidence: PMID:18976916 PAR-1 directly modulates apical-basal polarity between border cells and epithelial cells to promote detachment |
| GO:0005634 nucleus | IDA PMID:17507418 The Drosophila Lkb1 kinase is required for spindle formation... | KEEP AS NON CORE | Summary: LKB1 can localize to the nucleus per UniProt annotation. Reason: UniProt lists nucleus as a subcellular location. Supporting Evidence: file:genes/DROME/Lkb1/Lkb1-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. Nucleus PMID:17507418 The Drosophila Lkb1 kinase is required for spindle formation and asymmetric neuroblast division. |
| GO:0005737 cytoplasm | IDA PMID:17507418 The Drosophila Lkb1 kinase is required for spindle formation... | KEEP AS NON CORE | Summary: LKB1 localizes to the cytoplasm. Reason: UniProt lists cytoplasmic localization. Supporting Evidence: file:genes/DROME/Lkb1/Lkb1-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. PMID:17507418 The Drosophila Lkb1 kinase is required for spindle formation and asymmetric neuroblast division. |
| GO:0007052 mitotic spindle organization | IMP PMID:17507418 The Drosophila Lkb1 kinase is required for spindle formation... | KEEP AS NON CORE | Summary: LKB1 is required for spindle formation. Reason: Mutations disrupt spindle formation in larval brains. Supporting Evidence: PMID:17507418 mutations disrupt spindle formation, resulting in frequent polyploid cells in larval brains |
| GO:0055059 asymmetric neuroblast division | IMP PMID:17507418 The Drosophila Lkb1 kinase is required for spindle formation... | KEEP AS NON CORE | Summary: LKB1 is required for asymmetric neuroblast division. Reason: Mutations affect asymmetric division of larval neuroblasts. Supporting Evidence: PMID:17507418 dlkb1 mutations affect asymmetric division of larval neuroblasts |
| GO:0016325 oocyte microtubule cytoskeleton organization | IMP PMID:12540903 A role for Drosophila LKB1 in anterior-posterior axis format... | KEEP AS NON CORE | Summary: LKB1 controls repolarization of the oocyte cytoskeleton. Reason: Lkb1 is required for repolarization of the oocyte cytoskeleton. Supporting Evidence: PMID:12540903 homologue, lkb1, is required for the early A-P polarity of the oocyte |
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