id: Q8IPI4
gene_symbol: Mst27D
taxon:
  id: NCBITaxon:7227
  label: Drosophila melanogaster
status: COMPLETE
description: Microtubule binding is directly supported by CH-domain experiments, full-length localization
  and the bundling phenotype; plus-end-specific and polymerization-dynamics claims are unsupported
  EB1-family carry-over.
source_documents:
- genes/DROME/Mst27D/Mst27D-predictions-source.json
- genes/DROME/Mst27D/Mst27D-uniprot-source.json
- genes/DROME/Mst27D/Mst27D-hypotheses/microtubule-plus-end-capacity-and-polymerization/openscientist.md
references:
- id: PMID:37428798
  title: Nuclear elongation during spermiogenesis depends on physical linkage of nuclear pore complexes
    to bundled microtubules by Drosophila Mst27D.
  full_text_unavailable: false
  findings: []
- id: file:DROME/Mst27D/Mst27D-hypotheses/microtubule-plus-end-capacity-and-polymerization/openscientist.md
  title: OpenScientist audit of Mst27D microtubule plus-end and polymerization hypotheses
  findings:
  - statement: OpenScientist retained generic microtubule binding for Mst27D but identified plus-end,
      spindle and polymerization annotations as EB1/MAPRE-family over-propagation.
    supporting_text: Mst27D's plus-end localization, plus-end binding, spindle, and polymerization-regulation
      GO terms are **EB1/MAPRE-family IBA carry-over**, not evidence about Mst27D.
predictions:
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-09-08
  source_reference_id: file:DROME/Mst27D/Mst27D-predictions-source.json
  predicted_term:
    id: GO:0008017
    label: microtubule binding
  predicted_term_type: GO_MF
  review:
    assessment: CNN
    confidence_score: 2
    summary: Microtubule binding is directly supported by CH-domain experiments, full-length localization
      and the bundling phenotype. The same broad term already appears in GOA. Although GOA also contains
      plus-end binding, primary evidence shows that Mst27D binds along the lattice rather than sharing
      EB1’s preference, so the unsupported narrower annotation is not grounds for LSP. ProtNLM correctly
      captures the established broad activity. The OpenScientist audit also retained GO:0008017
      while identifying plus-end, spindle and dynamics terms as EB1/MAPRE-family IBA carry-over.
    supported_by:
    - reference_id: PMID:37428798
      supporting_text: Moreover, Mst27D binds all along MTs, contrasting with Eb1’s preference for growing
        MT plus ends.
    - reference_id: PMID:37428798
      supporting_text: In Mst27D null mutants, this bundling process does not occur and nuclear elongation
        is abnormal.
    - reference_id: file:DROME/Mst27D/Mst27D-hypotheses/microtubule-plus-end-capacity-and-polymerization/openscientist.md
      supporting_text: 'The primary experimental record for Mst27D ([PMID: 37428798](https://pubmed.ncbi.nlm.nih.gov/37428798/))
        demonstrates only four activities, none corresponding to plus-end tracking or dynamics regulation'