id: Q9VAS1
gene_symbol: Nepl19
taxon:
  id: NCBITaxon:7227
  label: Drosophila melanogaster
status: COMPLETE
description: Conventional metalloendopeptidase activity is refuted by specific disruption of the M13 catalytic
  apparatus.
source_documents:
- genes/DROME/Nepl19/Nepl19-predictions-source.json
- genes/DROME/Nepl19/Nepl19-uniprot-source.json
references:
- id: PMID:34189422
  title: Identification and bioinformatic analysis of neprilysin and neprilysin-like metalloendopeptidases
    in Drosophila melanogaster.
  findings: []
  full_text_unavailable: false
- id: file:DROME/Nepl19/Nepl19-bioinformatics/RESULTS.md
  title: DROME/Nepl19/Nepl19-bioinformatics/RESULTS.md
  findings: []
predictions:
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-09-08
  source_reference_id: file:DROME/Nepl19/Nepl19-predictions-source.json
  predicted_term:
    id: GO:0004222
    label: metalloendopeptidase activity
  predicted_term_type: GO_MF
  review:
    assessment: NPI
    confidence_score: 0
    summary: Conventional metalloendopeptidase activity is refuted by specific disruption of the M13 catalytic
      apparatus. Exact-sequence alignments map human H584/E585 to Nepl19 Q511/Q512, replacing a zinc ligand
      and catalytic glutamate; the downstream zinc-binding/catalytic region remains aligned. Two MAFFT
      strategies agree, and the active mouse control preserves all five sites. The published Nepl classification
      and existing NOT peptidase annotations agree with this independently reproduced sequence finding.
      This is pseudoenzyme overannotation, without a claim that the protein lacks a noncatalytic role.
    supported_by:
    - reference_id: file:DROME/Nepl19/Nepl19-bioinformatics/RESULTS.md
      supporting_text: The reference HEITH motif at 584–588 maps to QQLAH at Nepl19 511–515. Nepl19 retains
        the M13 fold and downstream ENIAD region but replaces the first zinc-binding histidine and catalytic
        glutamate with glutamines.
    - reference_id: PMID:34189422
      supporting_text: The 21 Nepl-genes encode proteins that exhibit significant similarity to neprilysins
        in their primary structure, but lack one or more of the motifs required for catalysis.
    error_type: PSEUDOENZYME_OVERANNOTATION