GATOR1 complex subunit that inhibits TORC1 signaling during amino acid limitation and nutrient stress.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0034198 cellular response to amino acid starvation | IBA GO_REF:0000033 | ACCEPT | Summary: Nprl2 participates in the response to amino-acid starvation. Reason: The Nprl2/Nprl3 complex mediates an adaptive response to amino-acid starvation. Supporting Evidence: PMID:24786828 The TORC1 inhibitors Nprl2 and Nprl3 mediate an adaptive response to amino-acid starvation in Drosophila. |
| GO:0005096 GTPase activator activity | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: GATOR1 has Rag GTPase GAP activity; Nprl2 is a GATOR1 subunit. Reason: GTPase-activating activity is established for GATOR1 (RagA/B), so the IBA is reasonable for Nprl2 but not the primary focus. Supporting Evidence: PMID:23723238 GATOR1 has GTPase-activating protein (GAP) activity for RagA and RagB |
| GO:1904262 negative regulation of TORC1 signaling | IBA GO_REF:0000033 | ACCEPT | Summary: Nprl2 inhibits TORC1 signaling, especially under amino-acid starvation. Reason: Both UniProt and the starvation study describe Nprl2-mediated inhibition of TORC1 in response to amino-acid limitation. Supporting Evidence: file:genes/DROME/Nprl2/Nprl2-uniprot.txt functions as an inhibitor of the amino acid-sensing branch of the TORC1 signaling pathway PMID:24786828 Nprl2 and Nprl3 inhibit TORC1 signaling in the female germline in response to amino-acid starvation. |
| GO:0005774 vacuolar membrane | IBA GO_REF:0000033 | MODIFY | Summary: The vacuolar membrane term is yeast-specific; Drosophila data indicate lysosomal localization. Reason: Replace vacuolar membrane with lysosome to match the Drosophila localization evidence. Proposed replacements: lysosome Supporting Evidence: PMID:24786828 Nprl2 and Nprl3 physically interact and are targeted to lysosomes and autolysosomes |
| GO:1990130 GATOR1 complex | IBA GO_REF:0000033 | ACCEPT | Summary: Nprl2 is a core subunit of the GATOR1 complex. Reason: UniProt annotates Nprl2 as a probable GATOR1 subcomplex component, supporting this complex membership. Supporting Evidence: file:genes/DROME/Nprl2/Nprl2-uniprot.txt probable component of the GATOR1 subcomplex which is likely composed of Iml1, Nplr2 and Nplr3 file:genes/DROME/Nprl2/Nprl2-deep-research-falcon.md Nprl2 is one of three core subunits of GATOR1 (with Iml1/DEPDC5 and Nprl3). |
| GO:0005737 cytoplasm | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: Nprl2 localizes to the cytoplasm. Reason: UniProt reports cytoplasmic localization. Supporting Evidence: file:genes/DROME/Nprl2/Nprl2-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm |
| GO:0005764 lysosome | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: Nprl2 localizes to lysosomes under nutrient stress. Reason: Lysosomal targeting is reported for Nprl2/Nprl3. Supporting Evidence: PMID:24786828 Nprl2 and Nprl3 physically interact and are targeted to lysosomes and autolysosomes |
| GO:0051301 cell division | IEA GO_REF:0000043 | MARK AS OVER ANNOTATED | Summary: General cell division is too broad relative to the specific mitotic-to-meiotic transition role. Reason: Evidence indicates a specific mitotic/meiotic transition control rather than broad cell division. Supporting Evidence: file:genes/DROME/Nprl2/Nprl2-uniprot.txt down-regulate TORC1 to slow cellular metabolism and promote the mitotic/meiotic transition |
| GO:0051321 meiotic cell cycle | IEA GO_REF:0000043 | MODIFY | Summary: Meiotic cell cycle is captured more precisely by germline mitotic-to-meiotic switching. Reason: Use the more specific germline cell cycle switching term supported by ovarian cyst data. Proposed replacements: germline cell cycle switching, mitotic to meiotic cell cycle Supporting Evidence: file:genes/DROME/Nprl2/Nprl2-uniprot.txt down-regulate TORC1 to slow cellular metabolism and promote the mitotic/meiotic transition |
| GO:1904262 negative regulation of TORC1 signaling | IEA GO_REF:0000117 | ACCEPT | Summary: Nprl2 inhibits TORC1 signaling, especially under amino-acid starvation. Reason: Both UniProt and the starvation study describe Nprl2-mediated inhibition of TORC1 in response to amino-acid limitation. Supporting Evidence: file:genes/DROME/Nprl2/Nprl2-uniprot.txt functions as an inhibitor of the amino acid-sensing branch of the TORC1 signaling pathway PMID:24786828 Nprl2 and Nprl3 inhibit TORC1 signaling in the female germline in response to amino-acid starvation. |
| GO:0034198 cellular response to amino acid starvation | NAS PMID:24786828 The TORC1 inhibitors Nprl2 and Nprl3 mediate an adaptive res... | ACCEPT | Summary: Nprl2 participates in the response to amino-acid starvation. Reason: The Nprl2/Nprl3 complex mediates an adaptive response to amino-acid starvation. Supporting Evidence: PMID:24786828 The TORC1 inhibitors Nprl2 and Nprl3 mediate an adaptive response to amino-acid starvation in Drosophila. |
| GO:1904262 negative regulation of TORC1 signaling | NAS PMID:24786828 The TORC1 inhibitors Nprl2 and Nprl3 mediate an adaptive res... | ACCEPT | Summary: Nprl2 inhibits TORC1 signaling, especially under amino-acid starvation. Reason: Both UniProt and the starvation study describe Nprl2-mediated inhibition of TORC1 in response to amino-acid limitation. Supporting Evidence: file:genes/DROME/Nprl2/Nprl2-uniprot.txt functions as an inhibitor of the amino acid-sensing branch of the TORC1 signaling pathway PMID:24786828 Nprl2 and Nprl3 inhibit TORC1 signaling in the female germline in response to amino-acid starvation. |
| GO:1904262 negative regulation of TORC1 signaling | IMP PMID:24786828 The TORC1 inhibitors Nprl2 and Nprl3 mediate an adaptive res... | ACCEPT | Summary: Nprl2 inhibits TORC1 signaling, especially under amino-acid starvation. Reason: Both UniProt and the starvation study describe Nprl2-mediated inhibition of TORC1 in response to amino-acid limitation. Supporting Evidence: file:genes/DROME/Nprl2/Nprl2-uniprot.txt functions as an inhibitor of the amino acid-sensing branch of the TORC1 signaling pathway PMID:24786828 Nprl2 and Nprl3 inhibit TORC1 signaling in the female germline in response to amino-acid starvation. |
| GO:0009267 cellular response to starvation | IMP PMID:27672113 The GATOR1 Complex Regulates Metabolic Homeostasis and the R... | KEEP AS NON CORE | Summary: Starvation response is supported but primarily in the amino-acid limitation context. Reason: Evidence supports amino-acid starvation response; the broader starvation term is acceptable but not core. Supporting Evidence: PMID:24786828 The TORC1 inhibitors Nprl2 and Nprl3 mediate an adaptive response to amino-acid starvation in Drosophila. PMID:27672113 The GATOR1 Complex Regulates Metabolic Homeostasis and the Response to Nutrient Stress in Drosophila melanogaster. |
| GO:0010898 positive regulation of triglyceride catabolic process | IMP PMID:27672113 The GATOR1 Complex Regulates Metabolic Homeostasis and the R... | MARK AS OVER ANNOTATED | Summary: Nprl2 affects TAG storage, but direct positive regulation of triglyceride catabolism is not shown. Reason: Reported phenotype is reduced TAG storage in mutants, which does not directly demonstrate increased triglyceride catabolism. Supporting Evidence: PMID:27672113 nprl2 and nprl3 mutant adults contain reduced amounts of stored TAG relative to wild-type animals |
| GO:1904262 negative regulation of TORC1 signaling | IGI PMID:27672113 The GATOR1 Complex Regulates Metabolic Homeostasis and the R... | ACCEPT | Summary: Nprl2 inhibits TORC1 signaling, especially under amino-acid starvation. Reason: Both UniProt and the starvation study describe Nprl2-mediated inhibition of TORC1 in response to amino-acid limitation. Supporting Evidence: file:genes/DROME/Nprl2/Nprl2-uniprot.txt functions as an inhibitor of the amino acid-sensing branch of the TORC1 signaling pathway PMID:24786828 Nprl2 and Nprl3 inhibit TORC1 signaling in the female germline in response to amino-acid starvation. PMID:27672113 The GATOR1 Complex Regulates Metabolic Homeostasis and the Response to Nutrient Stress in Drosophila melanogaster. |
| GO:0051729 germline cell cycle switching, mitotic to meiotic cell cycle | IGI PMID:25512509 TORC1 regulators Iml1/GATOR1 and GATOR2 control meiotic entr... | KEEP AS NON CORE | Summary: Nprl2 promotes the mitotic-to-meiotic transition in germline cysts via TORC1 inhibition. Reason: GATOR1 members down-regulate TORC1 to promote the mitotic/meiotic transition. Supporting Evidence: file:genes/DROME/Nprl2/Nprl2-uniprot.txt down-regulate TORC1 to slow cellular metabolism and promote the mitotic/meiotic transition PMID:25512509 TORC1 regulators Iml1/GATOR1 and GATOR2 control meiotic entry and oocyte development in Drosophila. |
| GO:1904262 negative regulation of TORC1 signaling | IMP PMID:27672113 The GATOR1 Complex Regulates Metabolic Homeostasis and the R... | ACCEPT | Summary: Nprl2 inhibits TORC1 signaling, especially under amino-acid starvation. Reason: Both UniProt and the starvation study describe Nprl2-mediated inhibition of TORC1 in response to amino-acid limitation. Supporting Evidence: file:genes/DROME/Nprl2/Nprl2-uniprot.txt functions as an inhibitor of the amino acid-sensing branch of the TORC1 signaling pathway PMID:24786828 Nprl2 and Nprl3 inhibit TORC1 signaling in the female germline in response to amino-acid starvation. PMID:27672113 The GATOR1 Complex Regulates Metabolic Homeostasis and the Response to Nutrient Stress in Drosophila melanogaster. |
| GO:0035859 Seh1-associated complex | IDA PMID:27166823 The GATOR2 Component Wdr24 Regulates TORC1 Activity and Lyso... | MODIFY | Summary: The SEA/GATOR complex term is broader than the specific GATOR1 complex membership. Reason: Evidence supports GATOR1 subcomplex membership; use the specific GATOR1 complex term. Proposed replacements: GATOR1 complex Supporting Evidence: file:genes/DROME/Nprl2/Nprl2-uniprot.txt Component of the GATOR complex consisting of mio, Nup44A/Seh1, Im11, Nplr3, Nplr2, Wdr24, Wdr59 and Sec13 PMID:27166823 The GATOR2 Component Wdr24 Regulates TORC1 Activity and Lysosome Function. |
| GO:1904262 negative regulation of TORC1 signaling | IGI PMID:27166823 The GATOR2 Component Wdr24 Regulates TORC1 Activity and Lyso... | ACCEPT | Summary: Nprl2 inhibits TORC1 signaling, especially under amino-acid starvation. Reason: Both UniProt and the starvation study describe Nprl2-mediated inhibition of TORC1 in response to amino-acid limitation. Supporting Evidence: file:genes/DROME/Nprl2/Nprl2-uniprot.txt functions as an inhibitor of the amino acid-sensing branch of the TORC1 signaling pathway PMID:24786828 Nprl2 and Nprl3 inhibit TORC1 signaling in the female germline in response to amino-acid starvation. PMID:27166823 The GATOR2 Component Wdr24 Regulates TORC1 Activity and Lysosome Function. |
| GO:1990130 GATOR1 complex | TAS PMID:27166823 The GATOR2 Component Wdr24 Regulates TORC1 Activity and Lyso... | ACCEPT | Summary: Nprl2 is a core subunit of the GATOR1 complex. Reason: UniProt annotates Nprl2 as a probable GATOR1 subcomplex component, supporting this complex membership. Supporting Evidence: file:genes/DROME/Nprl2/Nprl2-uniprot.txt probable component of the GATOR1 subcomplex which is likely composed of Iml1, Nplr2 and Nplr3 PMID:27166823 The GATOR2 Component Wdr24 Regulates TORC1 Activity and Lysosome Function. |
| GO:0032007 negative regulation of TOR signaling | IMP PMID:23723238 A Tumor suppressor complex with GAP activity for the Rag GTP... | MODIFY | Summary: This term is too general for the specific TORC1 inhibition shown for Nprl2. Reason: Evidence supports inhibition of TORC1 specifically; replace with the TORC1-specific term. Proposed replacements: negative regulation of TORC1 signaling Supporting Evidence: file:genes/DROME/Nprl2/Nprl2-uniprot.txt functions as an inhibitor of the amino acid-sensing branch of the TORC1 signaling pathway PMID:23723238 A Tumor suppressor complex with GAP activity for the Rag GTPases that signal amino acid sufficiency to mTORC1. |
| GO:0034198 cellular response to amino acid starvation | IMP PMID:23723238 A Tumor suppressor complex with GAP activity for the Rag GTP... | ACCEPT | Summary: Nprl2 participates in the response to amino-acid starvation. Reason: The Nprl2/Nprl3 complex mediates an adaptive response to amino-acid starvation. Supporting Evidence: PMID:24786828 The TORC1 inhibitors Nprl2 and Nprl3 mediate an adaptive response to amino-acid starvation in Drosophila. PMID:23723238 A Tumor suppressor complex with GAP activity for the Rag GTPases that signal amino acid sufficiency to mTORC1. |
| GO:0007293 germarium-derived egg chamber formation | IGI PMID:25512509 TORC1 regulators Iml1/GATOR1 and GATOR2 control meiotic entr... | MODIFY | Summary: Evidence points to oogenesis/meiotic entry roles rather than the specific egg chamber formation term. Reason: Use the broader oogenesis term supported by meiotic entry/oocyte development data. Proposed replacements: oogenesis Supporting Evidence: file:genes/DROME/Nprl2/Nprl2-uniprot.txt control meiotic entry and promote oocyte growth and development PMID:25512509 TORC1 regulators Iml1/GATOR1 and GATOR2 control meiotic entry and oocyte development in Drosophila. |
| GO:0005515 protein binding | IPI PMID:24786828 The TORC1 inhibitors Nprl2 and Nprl3 mediate an adaptive res... | KEEP AS NON CORE | Summary: Nprl2 physically interacts with Nprl3. Reason: Interaction evidence supports protein binding, but the term is generic and not core. Supporting Evidence: PMID:24786828 Nprl2 and Nprl3 physically interact |
| GO:0005634 nucleus | IDA PMID:24786828 The TORC1 inhibitors Nprl2 and Nprl3 mediate an adaptive res... | UNDECIDED | Summary: Nuclear localization is not supported by the accessible sources used for this review. Reason: Available curated localization statements emphasize cytoplasm/lysosome/autolysosome without nuclear evidence. Supporting Evidence: file:genes/DROME/Nprl2/Nprl2-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm. Lysosome PMID:24786828 The TORC1 inhibitors Nprl2 and Nprl3 mediate an adaptive response to amino-acid starvation in Drosophila. |
| GO:0005737 cytoplasm | IDA PMID:24786828 The TORC1 inhibitors Nprl2 and Nprl3 mediate an adaptive res... | KEEP AS NON CORE | Summary: Nprl2 localizes to the cytoplasm. Reason: UniProt reports cytoplasmic localization. Supporting Evidence: file:genes/DROME/Nprl2/Nprl2-uniprot.txt SUBCELLULAR LOCATION: Cytoplasm PMID:24786828 The TORC1 inhibitors Nprl2 and Nprl3 mediate an adaptive response to amino-acid starvation in Drosophila. |
| GO:0034198 cellular response to amino acid starvation | IMP PMID:24786828 The TORC1 inhibitors Nprl2 and Nprl3 mediate an adaptive res... | ACCEPT | Summary: Nprl2 participates in the response to amino-acid starvation. Reason: The Nprl2/Nprl3 complex mediates an adaptive response to amino-acid starvation. Supporting Evidence: PMID:24786828 The TORC1 inhibitors Nprl2 and Nprl3 mediate an adaptive response to amino-acid starvation in Drosophila. |
| GO:0044754 autolysosome | IDA PMID:24786828 The TORC1 inhibitors Nprl2 and Nprl3 mediate an adaptive res... | KEEP AS NON CORE | Summary: Nprl2 localizes to autolysosomes during amino-acid starvation. Reason: UniProt notes primary localization to autolysosomes under amino-acid starvation. Supporting Evidence: file:genes/DROME/Nprl2/Nprl2-uniprot.txt Localizes primarily to the autolysosomes during amino-acid starvation PMID:24786828 The TORC1 inhibitors Nprl2 and Nprl3 mediate an adaptive response to amino-acid starvation in Drosophila. |
| GO:0045792 negative regulation of cell size | IMP PMID:24786828 The TORC1 inhibitors Nprl2 and Nprl3 mediate an adaptive res... | KEEP AS NON CORE | Summary: Nprl2 restrains TORC1-dependent cell growth, consistent with negative regulation of cell size. Reason: GATOR1 inhibition of TORC1-dependent growth supports reduced cell size/growth phenotypes. Supporting Evidence: file:genes/DROME/Nprl2/Nprl2-uniprot.txt inhibiting TORC1-dependent cell growth PMID:24786828 The TORC1 inhibitors Nprl2 and Nprl3 mediate an adaptive response to amino-acid starvation in Drosophila. |
| GO:0048477 oogenesis | IMP PMID:24786828 The TORC1 inhibitors Nprl2 and Nprl3 mediate an adaptive res... | KEEP AS NON CORE | Summary: Nprl2 participates in oogenesis through GATOR1 control of meiotic entry and oocyte development. Reason: UniProt describes GATOR1 roles in meiotic entry and oocyte growth. Supporting Evidence: file:genes/DROME/Nprl2/Nprl2-uniprot.txt control meiotic entry and promote oocyte growth and development PMID:24786828 The TORC1 inhibitors Nprl2 and Nprl3 mediate an adaptive response to amino-acid starvation in Drosophila. |
| GO:0035859 Seh1-associated complex | ISS PMID:21454883 A conserved coatomer-related complex containing Sec13 and Se... | MODIFY | Summary: The SEA/GATOR complex term is broader than the specific GATOR1 complex membership. Reason: Evidence supports GATOR1 subcomplex membership; use the specific GATOR1 complex term. Proposed replacements: GATOR1 complex Supporting Evidence: file:genes/DROME/Nprl2/Nprl2-uniprot.txt Component of the GATOR complex consisting of mio, Nup44A/Seh1, Im11, Nplr3, Nplr2, Wdr24, Wdr59 and Sec13 PMID:21454883 A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae. |
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Download this section (compressed HTML)Nitrogen permease regulator-like 2 (Nprl2) encodes a component of the highly conserved GAP activity towards rags 1 (GATOR1) complex, which inhibits the product of mTor activity in response to amino acid starvation. The product of Nprl2, as well as the other GATOR1 components encoded by Nprl3 and Iml1, regulates early meiotic progression.
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