Pde4/dunce is a cAMP-specific phosphodiesterase that controls the amplitude and duration of cAMP signaling. The 983-residue product retains the PDE4 regulatory and catalytic architecture. In Drosophila neurons, dunce-dependent cAMP turnover shapes synaptic transmission, excitability, learning and memory; behavioral and developmental effects depend on the cells and isoforms in which the enzyme is expressed.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0001661 conditioned taste aversion | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Conditioned taste aversion is an aversive-learning phenotype. Reason: Larval aversive olfactory memory conditioned with quinine or salt depends on intact cAMP signaling. The term describes a behavioral paradigm rather than a separate core biochemical function. Supporting Evidence: PMID:19158309 larval aversive memory produced in this paradigm depends on intact cAMP signaling |
| GO:0001661 conditioned taste aversion | IMP PMID:19158309 Distinctive neuronal networks and biochemical pathways for a... | KEEP AS NON CORE | Summary: Conditioned taste aversion is an aversive-learning phenotype. Reason: Larval aversive olfactory memory conditioned with quinine or salt depends on intact cAMP signaling. The term describes a behavioral paradigm rather than a separate core biochemical function. Supporting Evidence: PMID:19158309 larval aversive memory produced in this paradigm depends on intact cAMP signaling |
| GO:0004114 3',5'-cyclic-nucleotide phosphodiesterase activity | IEA GO_REF:0000002 | MODIFY | Summary: The PDE4/dunce enzyme is cAMP-selective. Reason: The precise substrate is cAMP, established by genetic and biochemical characterization of dunce and supported by the intact PDE4 catalytic domain in this 983-residue product. The broad cyclic-nucleotide term does not assert cGMP specificity but can be refined. Proposed replacements: 3',5'-cyclic-AMP phosphodiesterase activity Supporting Evidence: PMID:14507965 Synaptic excitation is increased in genetic backgrounds that lack either a cAMP-specific phosphodiesterase |
| GO:0004115 3',5'-cyclic-AMP phosphodiesterase activity | IEA GO_REF:0000120 | ACCEPT | Summary: cAMP hydrolysis is the core enzymatic activity. Reason: The protein is a full catalytic-domain-containing PDE4/dunce isoform. The fly cAMP-specific phosphodiesterase phenotype and neuronal cAMP manipulation provide biological support independent of a broad phosphodiesterase family label. Supporting Evidence: PMID:14507965 Synaptic excitation is increased in genetic backgrounds that lack either a cAMP-specific phosphodiesterase |
| GO:0004115 3',5'-cyclic-AMP phosphodiesterase activity | ISS GO_REF:0000024 | ACCEPT | Summary: cAMP hydrolysis is the core enzymatic activity. Reason: The protein is a full catalytic-domain-containing PDE4/dunce isoform. The fly cAMP-specific phosphodiesterase phenotype and neuronal cAMP manipulation provide biological support independent of a broad phosphodiesterase family label. Supporting Evidence: PMID:14507965 Synaptic excitation is increased in genetic backgrounds that lack either a cAMP-specific phosphodiesterase |
| GO:0006198 cAMP catabolic process | IEA GO_REF:0000041 | ACCEPT | Summary: The enzyme directly catabolizes cAMP. Reason: Hydrolysis of cAMP to AMP terminates or shapes cAMP signaling; the physiological experiments explicitly manipulate cAMP catabolism in dunce-dependent neuronal excitability. Supporting Evidence: PMID:14507965 Synaptic excitation is increased in genetic backgrounds that lack either a cAMP-specific phosphodiesterase |
| GO:0007165 signal transduction | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: Signal transduction is a valid but very broad process annotation. Reason: cAMP degradation regulates neuronal signaling, but the direct biochemical role is captured by cAMP catabolism rather than a generic signaling label. Supporting Evidence: PMID:14507965 Synaptic excitation is increased in genetic backgrounds that lack either a cAMP-specific phosphodiesterase PMID:12040051 These mutations cause deficiencies in several learning paradigms and alter synaptic transmission, growth cone motility, and action potential generation. |
| GO:0007268 chemical synaptic transmission | IEA GO_REF:0000117 | ACCEPT | Summary: dunce-dependent cAMP turnover regulates synaptic transmission. Reason: Drosophila neuronal recordings show altered synaptic excitation and compensatory membrane excitability in dunce backgrounds, supporting a direct neuromodulatory role. The experiments are locus-level and do not establish that all alternatively spliced isoforms have identical expression. Supporting Evidence: PMID:14507965 Synaptic excitation is increased in genetic backgrounds that lack either a cAMP-specific phosphodiesterase PMID:12040051 These mutations cause deficiencies in several learning paradigms and alter synaptic transmission, growth cone motility, and action potential generation. |
| GO:0007268 chemical synaptic transmission | IMP PMID:12040051 Regional calcium regulation within cultured Drosophila neuro... | ACCEPT | Summary: dunce-dependent cAMP turnover regulates synaptic transmission. Reason: Drosophila neuronal recordings show altered synaptic excitation and compensatory membrane excitability in dunce backgrounds, supporting a direct neuromodulatory role. The experiments are locus-level and do not establish that all alternatively spliced isoforms have identical expression. Supporting Evidence: PMID:14507965 Synaptic excitation is increased in genetic backgrounds that lack either a cAMP-specific phosphodiesterase PMID:12040051 These mutations cause deficiencies in several learning paradigms and alter synaptic transmission, growth cone motility, and action potential generation. |
| GO:0007268 chemical synaptic transmission | IMP PMID:14507965 Postsynaptic protein kinase A reduces neuronal excitability ... | ACCEPT | Summary: dunce-dependent cAMP turnover regulates synaptic transmission. Reason: Drosophila neuronal recordings show altered synaptic excitation and compensatory membrane excitability in dunce backgrounds, supporting a direct neuromodulatory role. The experiments are locus-level and do not establish that all alternatively spliced isoforms have identical expression. Supporting Evidence: PMID:14507965 Synaptic excitation is increased in genetic backgrounds that lack either a cAMP-specific phosphodiesterase PMID:12040051 These mutations cause deficiencies in several learning paradigms and alter synaptic transmission, growth cone motility, and action potential generation. |
| GO:0007612 learning | IMP PMID:16135747 Induction of cAMP response element-binding protein-dependent... | KEEP AS NON CORE | Summary: Learning depends on dunce-regulated cAMP signaling. Reason: Mutant analyses and neuronal experiments connect altered cAMP turnover with multiple learning paradigms. The effect is mechanistically grounded but downstream of the PDE activity. Supporting Evidence: PMID:12040051 These mutations cause deficiencies in several learning paradigms and alter synaptic transmission, growth cone motility, and action potential generation. |
| GO:0007613 memory | IMP PMID:10970024 Identification of Drosophila mutant with memory defects afte... | KEEP AS NON CORE | Summary: Memory defects are compatible with the established dunce phenotype. Reason: The annotated study compares new mutants with known dnc short-term-memory mutants; independent work directly establishes dunce-sensitive memory circuitry. This supports a locus-level memory role while leaving this isoform-specific expression unresolved. Supporting Evidence: PMID:23197709 dnc-sensitive cAMP signals support ARM within antennal lobe local neurons (LNs) and KCs. |
| GO:0007614 short-term memory | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Short-lived larval aversive memory requires intact cAMP signaling. Reason: The cited larval conditioning study demonstrates a cAMP-dependent short-lived memory component. The annotation is retained as a behavioral outcome rather than a separate enzyme mechanism. Supporting Evidence: PMID:19158309 larval aversive memory produced in this paradigm depends on intact cAMP signaling |
| GO:0007614 short-term memory | IMP PMID:19158309 Distinctive neuronal networks and biochemical pathways for a... | KEEP AS NON CORE | Summary: Short-lived larval aversive memory requires intact cAMP signaling. Reason: The cited larval conditioning study demonstrates a cAMP-dependent short-lived memory component. The annotation is retained as a behavioral outcome rather than a separate enzyme mechanism. Supporting Evidence: PMID:19158309 larval aversive memory produced in this paradigm depends on intact cAMP signaling |
| GO:0007615 anesthesia-resistant memory | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Anesthesia-resistant memory depends on dunce-sensitive cAMP signals. Reason: The primary paper localizes this requirement to antennal lobe local neurons and mushroom body Kenyon cells. It is a circuit-level consequence of the core cAMP-regulatory enzyme activity. Supporting Evidence: PMID:23197709 dnc-sensitive cAMP signals support ARM within antennal lobe local neurons (LNs) and KCs. |
| GO:0007615 anesthesia-resistant memory | IMP PMID:23197709 Consolidated and labile odor memory are separately encoded w... | KEEP AS NON CORE | Summary: Anesthesia-resistant memory depends on dunce-sensitive cAMP signals. Reason: The primary paper localizes this requirement to antennal lobe local neurons and mushroom body Kenyon cells. It is a circuit-level consequence of the core cAMP-regulatory enzyme activity. Supporting Evidence: PMID:23197709 dnc-sensitive cAMP signals support ARM within antennal lobe local neurons (LNs) and KCs. |
| GO:0007617 mating behavior | TAS PMID:14504657 Sex-peptides: seminal peptides of the Drosophila male. | UNDECIDED | Summary: The precise mating behavior assertion requires the cited full-text context. Reason: The available review abstracts do not expose the target-specific evidence for mating behavior. A broad role of dunce in neuronal cAMP signaling is established, but that is insufficient to verify this particular behavioral annotation or its applicability to the selected long isoform. The annotation is not rejected on the basis of an abstract emphasizing another topic. Supporting Evidence: PMID:14507965 Synaptic excitation is increased in genetic backgrounds that lack either a cAMP-specific phosphodiesterase |
| GO:0007619 courtship behavior | IEA GO_REF:0000117 | UNDECIDED | Summary: The precise courtship behavior assertion requires the cited full-text context. Reason: The available review abstracts do not expose the target-specific evidence for courtship behavior. A broad role of dunce in neuronal cAMP signaling is established, but that is insufficient to verify this particular behavioral annotation or its applicability to the selected long isoform. The annotation is not rejected on the basis of an abstract emphasizing another topic. Supporting Evidence: PMID:14507965 Synaptic excitation is increased in genetic backgrounds that lack either a cAMP-specific phosphodiesterase |
| GO:0007619 courtship behavior | TAS PMID:11092827 Courtship in Drosophila. | UNDECIDED | Summary: The precise courtship behavior assertion requires the cited full-text context. Reason: The available review abstracts do not expose the target-specific evidence for courtship behavior. A broad role of dunce in neuronal cAMP signaling is established, but that is insufficient to verify this particular behavioral annotation or its applicability to the selected long isoform. The annotation is not rejected on the basis of an abstract emphasizing another topic. Supporting Evidence: PMID:14507965 Synaptic excitation is increased in genetic backgrounds that lack either a cAMP-specific phosphodiesterase |
| GO:0007619 courtship behavior | TAS PMID:11715043 Drosophila: genetics meets behaviour. | UNDECIDED | Summary: The precise courtship behavior assertion requires the cited full-text context. Reason: The available review abstracts do not expose the target-specific evidence for courtship behavior. A broad role of dunce in neuronal cAMP signaling is established, but that is insufficient to verify this particular behavioral annotation or its applicability to the selected long isoform. The annotation is not rejected on the basis of an abstract emphasizing another topic. Supporting Evidence: PMID:14507965 Synaptic excitation is increased in genetic backgrounds that lack either a cAMP-specific phosphodiesterase |
| GO:0007619 courtship behavior | TAS PMID:12486700 Genetic basis of male sexual behavior. | UNDECIDED | Summary: The precise courtship behavior assertion requires the cited full-text context. Reason: The available review abstracts do not expose the target-specific evidence for courtship behavior. A broad role of dunce in neuronal cAMP signaling is established, but that is insufficient to verify this particular behavioral annotation or its applicability to the selected long isoform. The annotation is not rejected on the basis of an abstract emphasizing another topic. Supporting Evidence: PMID:14507965 Synaptic excitation is increased in genetic backgrounds that lack either a cAMP-specific phosphodiesterase |
| GO:0007623 circadian rhythm | IEA GO_REF:0000117 | UNDECIDED | Summary: The precise circadian rhythm assertion requires the cited full-text context. Reason: The available review abstracts do not expose the target-specific evidence for circadian rhythm. A broad role of dunce in neuronal cAMP signaling is established, but that is insufficient to verify this particular behavioral annotation or its applicability to the selected long isoform. The annotation is not rejected on the basis of an abstract emphasizing another topic. Supporting Evidence: PMID:14507965 Synaptic excitation is increased in genetic backgrounds that lack either a cAMP-specific phosphodiesterase |
| GO:0007623 circadian rhythm | TAS PMID:12486701 Genetic analysis of the circadian system in Drosophila melan... | UNDECIDED | Summary: The precise circadian rhythm assertion requires the cited full-text context. Reason: The available review abstracts do not expose the target-specific evidence for circadian rhythm. A broad role of dunce in neuronal cAMP signaling is established, but that is insufficient to verify this particular behavioral annotation or its applicability to the selected long isoform. The annotation is not rejected on the basis of an abstract emphasizing another topic. Supporting Evidence: PMID:14507965 Synaptic excitation is increased in genetic backgrounds that lack either a cAMP-specific phosphodiesterase |
| GO:0008081 phosphoric diester hydrolase activity | IEA GO_REF:0000002 | MODIFY | Summary: cAMP phosphodiesterase activity specifies the hydrolase class. Reason: The intact PDE4 domain and fly cAMP-specific phenotype support the narrower substrate-defined reaction. Proposed replacements: 3',5'-cyclic-AMP phosphodiesterase activity Supporting Evidence: PMID:14507965 Synaptic excitation is increased in genetic backgrounds that lack either a cAMP-specific phosphodiesterase |
| GO:0008306 associative learning | IMP PMID:10970024 Identification of Drosophila mutant with memory defects afte... | KEEP AS NON CORE | Summary: Associative memory is consistent with dunce-dependent cAMP circuitry. Reason: Independent direct fly work shows that dunce-sensitive cAMP signals support an olfactory memory component. The cited comparator study alone is not the strongest biochemical evidence. Supporting Evidence: PMID:23197709 dnc-sensitive cAMP signals support ARM within antennal lobe local neurons (LNs) and KCs. |
| GO:0008355 olfactory learning | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Olfactory learning is a well-supported downstream role of dunce. Reason: Direct olfactory-conditioning work resolves dunce-sensitive cAMP signals in antennal-lobe and mushroom-body circuitry. This supports the behavioral role recorded in the reviews while retaining cAMP hydrolysis as the molecular mechanism. Supporting Evidence: PMID:23197709 dnc-sensitive cAMP signals support ARM within antennal lobe local neurons (LNs) and KCs. |
| GO:0008355 olfactory learning | TAS PMID:11462211 Molecular biology and anatomy of Drosophila olfactory associ... | KEEP AS NON CORE | Summary: Olfactory learning is a well-supported downstream role of dunce. Reason: Direct olfactory-conditioning work resolves dunce-sensitive cAMP signals in antennal-lobe and mushroom-body circuitry. This supports the behavioral role recorded in the reviews while retaining cAMP hydrolysis as the molecular mechanism. Supporting Evidence: PMID:23197709 dnc-sensitive cAMP signals support ARM within antennal lobe local neurons (LNs) and KCs. |
| GO:0008355 olfactory learning | TAS PMID:11715043 Drosophila: genetics meets behaviour. | KEEP AS NON CORE | Summary: Olfactory learning is a well-supported downstream role of dunce. Reason: Direct olfactory-conditioning work resolves dunce-sensitive cAMP signals in antennal-lobe and mushroom-body circuitry. This supports the behavioral role recorded in the reviews while retaining cAMP hydrolysis as the molecular mechanism. Supporting Evidence: PMID:23197709 dnc-sensitive cAMP signals support ARM within antennal lobe local neurons (LNs) and KCs. |
| GO:0040040 thermosensory behavior | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Thermal preference depends on the cAMP-PKA pathway. Reason: The fly temperature-preference study links mutant behavior to cAMP/PKA activity and neuronal rescue. This supports a behavioral role without implying that PDE4 is itself a thermoreceptor. Supporting Evidence: PMID:18594510 Preferred temperatures were affected by the level of cAMP and PKA activity in the MBs in various PKA pathway mutants. |
| GO:0040040 thermosensory behavior | IMP PMID:18594510 cAMP signalling in mushroom bodies modulates temperature pre... | KEEP AS NON CORE | Summary: Thermal preference depends on the cAMP-PKA pathway. Reason: The fly temperature-preference study links mutant behavior to cAMP/PKA activity and neuronal rescue. This supports a behavioral role without implying that PDE4 is itself a thermoreceptor. Supporting Evidence: PMID:18594510 Preferred temperatures were affected by the level of cAMP and PKA activity in the MBs in various PKA pathway mutants. |
| GO:0045202 synapse | IEA GO_REF:0000108 | ACCEPT | Summary: Synaptic localization is compatible with the established neuronal function. Reason: The inherited location is supported by dunce-sensitive cAMP signaling in defined synaptic circuits and by changes in synaptic transmission. This does not establish constitutive association of every isoform with all synapses. Supporting Evidence: PMID:12040051 These mutations cause deficiencies in several learning paradigms and alter synaptic transmission, growth cone motility, and action potential generation. PMID:23197709 dnc-sensitive cAMP signals support ARM within antennal lobe local neurons (LNs) and KCs. |
| GO:0046958 nonassociative learning | IEA GO_REF:0000117 | UNDECIDED | Summary: The precise nonassociative learning assertion requires the cited full-text context. Reason: The available review abstracts do not expose the target-specific evidence for nonassociative learning. A broad role of dunce in neuronal cAMP signaling is established, but that is insufficient to verify this particular behavioral annotation or its applicability to the selected long isoform. The annotation is not rejected on the basis of an abstract emphasizing another topic. Supporting Evidence: PMID:14507965 Synaptic excitation is increased in genetic backgrounds that lack either a cAMP-specific phosphodiesterase |
| GO:0046958 nonassociative learning | TAS PMID:14504657 Sex-peptides: seminal peptides of the Drosophila male. | UNDECIDED | Summary: The precise nonassociative learning assertion requires the cited full-text context. Reason: The available review abstracts do not expose the target-specific evidence for nonassociative learning. A broad role of dunce in neuronal cAMP signaling is established, but that is insufficient to verify this particular behavioral annotation or its applicability to the selected long isoform. The annotation is not rejected on the basis of an abstract emphasizing another topic. Supporting Evidence: PMID:14507965 Synaptic excitation is increased in genetic backgrounds that lack either a cAMP-specific phosphodiesterase |
| GO:0048675 axon extension | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Axonal arborization is altered by dunce-dependent cAMP levels. Reason: The primary study explicitly reports enhanced terminal arborization in dunce mutants. The developmental phenotype follows cAMP regulation and is not a separate structural outgrowth activity of the enzyme. Supporting Evidence: PMID:14960616 Elevated cAMP levels in dunce mutants with reduced phosphodiesterase activity also cause enhanced arborization. |
| GO:0048675 axon extension | IMP PMID:14960616 Neuronal activity and adenylyl cyclase in environment-depend... | KEEP AS NON CORE | Summary: Axonal arborization is altered by dunce-dependent cAMP levels. Reason: The primary study explicitly reports enhanced terminal arborization in dunce mutants. The developmental phenotype follows cAMP regulation and is not a separate structural outgrowth activity of the enzyme. Supporting Evidence: PMID:14960616 Elevated cAMP levels in dunce mutants with reduced phosphodiesterase activity also cause enhanced arborization. |
Loading supporting content…
Download this section (compressed HTML)Loading supporting content…
Download this section (compressed HTML)Loading supporting content…
Download this section (compressed HTML)