Ser (Serrate) encodes a DSL-family transmembrane ligand of the Drosophila Notch receptor, the ortholog of vertebrate Jagged. Its extracellular region contains an N-terminal MNNL/DSL domain, 14 EGF-like repeats and a cysteine-rich region. Serrate binds Notch EGF repeats 11-12 with an affinity similar to Delta, and its signaling competence depends on Mind bomb/Neuralized-mediated ubiquitination and endocytosis via a conserved intracellular motif. Serrate also cis-inhibits Notch in the same cell. Glycosylation of Notch by Fringe reduces responsiveness to Serrate while potentiating Delta signaling, which restricts Serrate-Notch signaling to the dorsal/ventral boundary of the wing disc. Serrate signaling contributes to wing margin formation, leg segmentation, eye development, embryonic denticle patterning, salivary gland and tracheal development, glial proliferation and crystal cell specification during hematopoiesis.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005112 Notch binding | IBA GO_REF:0000033 | ACCEPT | Summary: Serrate binds Notch in trans via the same EGF repeats (11-12) of Notch that bind Delta (IBA). Reason: Direct Serrate-Notch binding is shown by cell aggregation and soluble ectodomain binding assays, with affinity similar to Delta-Notch; binding is modulated by Ofut1 and Fringe. Core molecular function of the ligand. Supporting Evidence: PMID:1657403 We report a novel molecular interaction between Notch and Serrate, another EGF-homologous transmembrane protein containing a region of striking similarity to Delta, and show that the same two EGF repeats of Notch also constitute a Serrate binding domain. PMID:10504334 Contrary to previous suggestions, our evidence implies that the interactions between Serrate and Notch are similar in affinity to those between Delta and Notch. |
| GO:0005112 Notch binding | IDA PMID:10504334 Ligand-receptor interactions and trans-endocytosis of Delta,... | ACCEPT | Summary: Serrate binds Notch in trans via the same EGF repeats (11-12) of Notch that bind Delta (IDA). Reason: Direct Serrate-Notch binding is shown by cell aggregation and soluble ectodomain binding assays, with affinity similar to Delta-Notch; binding is modulated by Ofut1 and Fringe. Core molecular function of the ligand. Supporting Evidence: PMID:10504334 Ligand-receptor interactions and trans-endocytosis of Delta, Serrate and Notch: members of the Notch signalling pathway in Drosophila PMID:1657403 We report a novel molecular interaction between Notch and Serrate, another EGF-homologous transmembrane protein containing a region of striking similarity to Delta, and show that the same two EGF repeats of Notch also constitute a Serrate binding domain. |
| GO:0005112 Notch binding | IDA PMID:12909620 Modulation of notch-ligand binding by protein O-fucosyltrans... | ACCEPT | Summary: Serrate binds Notch in trans via the same EGF repeats (11-12) of Notch that bind Delta (IDA). Reason: Direct Serrate-Notch binding is shown by cell aggregation and soluble ectodomain binding assays, with affinity similar to Delta-Notch; binding is modulated by Ofut1 and Fringe. Core molecular function of the ligand. Supporting Evidence: PMID:12909620 To investigate the molecular basis for the requirement for O-linked fucose on Notch, we assayed the ability of tagged, soluble forms of the Notch extracellular domain to bind to its ligands, Delta and Serrate PMID:1657403 We report a novel molecular interaction between Notch and Serrate, another EGF-homologous transmembrane protein containing a region of striking similarity to Delta, and show that the same two EGF repeats of Notch also constitute a Serrate binding domain. PMID:10504334 Contrary to previous suggestions, our evidence implies that the interactions between Serrate and Notch are similar in affinity to those between Delta and Notch. |
| GO:0005112 Notch binding | IDA PMID:17923477 In vitro reconstitution of the modulation of Drosophila Notc... | ACCEPT | Summary: Serrate binds Notch in trans via the same EGF repeats (11-12) of Notch that bind Delta (IDA). Reason: Direct Serrate-Notch binding is shown by cell aggregation and soluble ectodomain binding assays, with affinity similar to Delta-Notch; binding is modulated by Ofut1 and Fringe. Core molecular function of the ligand. Supporting Evidence: PMID:17923477 In vitro glycosylation and ligand binding studies establish that the addition of N-acetylglucosamine onto O-fucose in vitro is sufficient both to enhance Notch binding to the Delta ligand and to inhibit Notch binding to the Serrate ligand PMID:1657403 We report a novel molecular interaction between Notch and Serrate, another EGF-homologous transmembrane protein containing a region of striking similarity to Delta, and show that the same two EGF repeats of Notch also constitute a Serrate binding domain. PMID:10504334 Contrary to previous suggestions, our evidence implies that the interactions between Serrate and Notch are similar in affinity to those between Delta and Notch. |
| GO:0005112 Notch binding | IEA GO_REF:0000002 | ACCEPT | Summary: Serrate binds Notch in trans via the same EGF repeats (11-12) of Notch that bind Delta (IEA). Reason: Direct Serrate-Notch binding is shown by cell aggregation and soluble ectodomain binding assays, with affinity similar to Delta-Notch; binding is modulated by Ofut1 and Fringe. Core molecular function of the ligand. Supporting Evidence: PMID:1657403 We report a novel molecular interaction between Notch and Serrate, another EGF-homologous transmembrane protein containing a region of striking similarity to Delta, and show that the same two EGF repeats of Notch also constitute a Serrate binding domain. PMID:10504334 Contrary to previous suggestions, our evidence implies that the interactions between Serrate and Notch are similar in affinity to those between Delta and Notch. |
| GO:0005112 Notch binding | IPI PMID:20203305 Gene deletion screen for cardiomyopathy in adult Drosophila ... | ACCEPT | Summary: Serrate binds Notch in trans via the same EGF repeats (11-12) of Notch that bind Delta (IPI). Reason: Direct Serrate-Notch binding is shown by cell aggregation and soluble ectodomain binding assays, with affinity similar to Delta-Notch; binding is modulated by Ofut1 and Fringe. Core molecular function of the ligand. Supporting Evidence: PMID:20203305 To test this hypothesis, we conducted cell-aggregation assays with cells expressing Notch, Delta, Serrate or Wry and examined the ability of Notch to bind different ligands PMID:1657403 We report a novel molecular interaction between Notch and Serrate, another EGF-homologous transmembrane protein containing a region of striking similarity to Delta, and show that the same two EGF repeats of Notch also constitute a Serrate binding domain. PMID:10504334 Contrary to previous suggestions, our evidence implies that the interactions between Serrate and Notch are similar in affinity to those between Delta and Notch. |
| GO:0005509 calcium ion binding | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: Calcium ion binding inferred from calcium-binding EGF-like domains (InterPro). Reason: Structural property of the EGF repeats; not the core function. |
| GO:0005515 protein binding | IPI PMID:15829515 The ubiquitin ligase Drosophila Mind bomb promotes Notch sig... | REMOVE | Summary: Generic protein binding (IPI) with the E3 ubiquitin ligase Mind bomb. Reason: Uninformative; Serrate is the ubiquitination substrate of Mib1. The functionally relevant ligand activity is captured by Notch binding. Removal does not imply the interaction is false. Supporting Evidence: PMID:15829515 The ubiquitin ligase Drosophila Mind bomb promotes Notch signaling by regulating the localization and activity of Serrate and Delta |
| GO:0005771 multivesicular body | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: Serrate is endocytosed and found in vesicles, endosomes and multivesicular bodies. Reason: Mib1/Neuralized-dependent endocytosis is required for Serrate signaling competence and also mediates turnover. Endosomal localization is documented but regulatory; non-core. Supporting Evidence: PMID:20176925 Endocytosis of the transmembrane ligands Delta (Dl) and Serrate (Ser) is required for the proper activation of Notch receptors. |
| GO:0005886 plasma membrane | IDA PMID:16643882 Notch pathway repression by vestigial is required to promote... | ACCEPT | Summary: Serrate is a single-pass type I transmembrane protein at the plasma membrane, enriched apically in epithelia (IDA). Reason: Surface presentation is required for trans-activation of Notch on neighbouring cells. Supporting Evidence: PMID:16643882 Moreover, close examination of the cellular location of several of the main actors of the N pathway (Notch, Delta, neuralized, Serrate, Mind bomb1 and fringe) during dorsal longitudinal muscle development enabled us to find that Notch receptor can play multiple roles in adult myogenesis PMID:1840519 Antibodies directed against part of the extracellular region confirm the localization of the protein in the membrane. |
| GO:0005886 plasma membrane | IDA PMID:1840519 The Drosophila gene Serrate encodes an EGF-like transmembran... | ACCEPT | Summary: Serrate is a single-pass type I transmembrane protein at the plasma membrane, enriched apically in epithelia (IDA). Reason: Surface presentation is required for trans-activation of Notch on neighbouring cells. Supporting Evidence: PMID:1840519 The Drosophila gene Serrate encodes an EGF-like transmembrane protein with a complex expression pattern in embryos and wing discs |
| GO:0005886 plasma membrane | IEA GO_REF:0000044 | ACCEPT | Summary: Serrate is a single-pass type I transmembrane protein at the plasma membrane, enriched apically in epithelia (IEA). Reason: Surface presentation is required for trans-activation of Notch on neighbouring cells. Supporting Evidence: PMID:1840519 Antibodies directed against part of the extracellular region confirm the localization of the protein in the membrane. |
| GO:0005886 plasma membrane | TAS Reactome:R-DME-2076706 | ACCEPT | Summary: Serrate is a single-pass type I transmembrane protein at the plasma membrane, enriched apically in epithelia (TAS). Reason: Surface presentation is required for trans-activation of Notch on neighbouring cells. Supporting Evidence: PMID:1840519 Antibodies directed against part of the extracellular region confirm the localization of the protein in the membrane. |
| GO:0005886 plasma membrane | TAS Reactome:R-DME-209226 | ACCEPT | Summary: Serrate is a single-pass type I transmembrane protein at the plasma membrane, enriched apically in epithelia (TAS). Reason: Surface presentation is required for trans-activation of Notch on neighbouring cells. Supporting Evidence: PMID:1840519 Antibodies directed against part of the extracellular region confirm the localization of the protein in the membrane. |
| GO:0005938 cell cortex | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: Serrate detected at the apical cortex of wing imaginal cells. Reason: Correct but less informative than apical plasma membrane; non-core. |
| GO:0007154 cell communication | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: Electronic annotation to cell communication. Reason: Correct but very general; the specific process is Notch signaling. |
| GO:0007166 cell surface receptor signaling pathway | IEA GO_REF:0000117 | MODIFY | Summary: Electronic annotation to cell surface receptor signaling pathway. Reason: Correct but generic; Serrate acts specifically in the Notch signaling pathway (GO:0007219), already annotated with experimental evidence. Proposed replacements: Notch signaling pathway Supporting Evidence: PMID:9202123 Ser and Dl encode transmembrane ligands for Notch, whereas fng encodes a pioneer protein. |
| GO:0007219 Notch signaling pathway | IEA GO_REF:0000002 | ACCEPT | Summary: Serrate is a Notch ligand and a core component of the Notch signaling pathway (IEA). Reason: Serrate binds and activates Notch; its signaling competence requires Mib1/Neuralized-dependent endocytosis. Participation in the Notch pathway is core. Supporting Evidence: PMID:9202123 Ser and Dl encode transmembrane ligands for Notch, whereas fng encodes a pioneer protein. PMID:20176925 Endocytosis of the transmembrane ligands Delta (Dl) and Serrate (Ser) is required for the proper activation of Notch receptors. |
| GO:0007219 Notch signaling pathway | IGI PMID:35264151 Ubiquitylation is required for the incorporation of the Notc... | ACCEPT | Summary: Serrate is a Notch ligand and a core component of the Notch signaling pathway (IGI). Reason: Serrate binds and activates Notch; its signaling competence requires Mib1/Neuralized-dependent endocytosis. Participation in the Notch pathway is core. Supporting Evidence: PMID:35264151 Due to relief of cis-inhibition, N signalling is induced in the Dl Ser double mutant cells at the clone boundary (arrowhead) PMID:9202123 Ser and Dl encode transmembrane ligands for Notch, whereas fng encodes a pioneer protein. PMID:20176925 Endocytosis of the transmembrane ligands Delta (Dl) and Serrate (Ser) is required for the proper activation of Notch receptors. |
| GO:0007219 Notch signaling pathway | IGI PMID:9310337 A dominant-negative form of Serrate acts as a general antago... | ACCEPT | Summary: Serrate is a Notch ligand and a core component of the Notch signaling pathway (IGI). Reason: Serrate binds and activates Notch; its signaling competence requires Mib1/Neuralized-dependent endocytosis. Participation in the Notch pathway is core. Supporting Evidence: PMID:9310337 A dominant-negative form of Serrate acts as a general antagonist of Notch activation PMID:9202123 Ser and Dl encode transmembrane ligands for Notch, whereas fng encodes a pioneer protein. PMID:20176925 Endocytosis of the transmembrane ligands Delta (Dl) and Serrate (Ser) is required for the proper activation of Notch receptors. |
| GO:0007219 Notch signaling pathway | IGI PMID:9553038 Delta and Serrate are redundant Notch ligands required for a... | ACCEPT | Summary: Serrate is a Notch ligand and a core component of the Notch signaling pathway (IGI). Reason: Serrate binds and activates Notch; its signaling competence requires Mib1/Neuralized-dependent endocytosis. Participation in the Notch pathway is core. Supporting Evidence: PMID:9553038 Delta and Serrate are redundant Notch ligands required for asymmetric cell divisions within the Drosophila sensory organ lineage PMID:9202123 Ser and Dl encode transmembrane ligands for Notch, whereas fng encodes a pioneer protein. PMID:20176925 Endocytosis of the transmembrane ligands Delta (Dl) and Serrate (Ser) is required for the proper activation of Notch receptors. |
| GO:0007219 Notch signaling pathway | IMP PMID:16093323 The interplay between DSL proteins and ubiquitin ligases in ... | ACCEPT | Summary: Serrate is a Notch ligand and a core component of the Notch signaling pathway (IMP). Reason: Serrate binds and activates Notch; its signaling competence requires Mib1/Neuralized-dependent endocytosis. Participation in the Notch pathway is core. Supporting Evidence: PMID:16093323 Simultaneous absence of neuralized and mib1 completely abolishes Notch signaling in both aforementioned contexts, making it likely that ubiquitination is a prerequisite for Delta/Serrate signaling PMID:9202123 Ser and Dl encode transmembrane ligands for Notch, whereas fng encodes a pioneer protein. PMID:20176925 Endocytosis of the transmembrane ligands Delta (Dl) and Serrate (Ser) is required for the proper activation of Notch receptors. |
| GO:0007219 Notch signaling pathway | IMP PMID:16125166 Serrate-Notch signaling defines the scope of the initial den... | ACCEPT | Summary: Serrate is a Notch ligand and a core component of the Notch signaling pathway (IMP). Reason: Serrate binds and activates Notch; its signaling competence requires Mib1/Neuralized-dependent endocytosis. Participation in the Notch pathway is core. Supporting Evidence: PMID:16125166 Serrate-Notch signaling defines the scope of the initial denticle field by modulating EGFR activation PMID:9202123 Ser and Dl encode transmembrane ligands for Notch, whereas fng encodes a pioneer protein. PMID:20176925 Endocytosis of the transmembrane ligands Delta (Dl) and Serrate (Ser) is required for the proper activation of Notch receptors. |
| GO:0007219 Notch signaling pathway | IMP PMID:1657403 Specific EGF repeats of Notch mediate interactions with Delt... | ACCEPT | Summary: Serrate is a Notch ligand and a core component of the Notch signaling pathway (IMP). Reason: Serrate binds and activates Notch; its signaling competence requires Mib1/Neuralized-dependent endocytosis. Participation in the Notch pathway is core. Supporting Evidence: PMID:1657403 Specific EGF repeats of Notch mediate interactions with Delta and Serrate: implications for Notch as a multifunctional receptor PMID:9202123 Ser and Dl encode transmembrane ligands for Notch, whereas fng encodes a pioneer protein. PMID:20176925 Endocytosis of the transmembrane ligands Delta (Dl) and Serrate (Ser) is required for the proper activation of Notch receptors. |
| GO:0007219 Notch signaling pathway | IMP PMID:20203305 Gene deletion screen for cardiomyopathy in adult Drosophila ... | ACCEPT | Summary: Serrate is a Notch ligand and a core component of the Notch signaling pathway (IMP). Reason: Serrate binds and activates Notch; its signaling competence requires Mib1/Neuralized-dependent endocytosis. Participation in the Notch pathway is core. Supporting Evidence: PMID:20203305 Wry contains EGF repeats and a transmembrane domain but does not possess a DSL (Delta-Serrate-Lag) domain (Online Figure IV), suggesting that it may be involved in Notch signaling PMID:9202123 Ser and Dl encode transmembrane ligands for Notch, whereas fng encodes a pioneer protein. PMID:20176925 Endocytosis of the transmembrane ligands Delta (Dl) and Serrate (Ser) is required for the proper activation of Notch receptors. |
| GO:0007219 Notch signaling pathway | IMP PMID:23970418 A Serrate-Notch-Canoe complex mediates essential interaction... | ACCEPT | Summary: Serrate is a Notch ligand and a core component of the Notch signaling pathway (IMP). Reason: Serrate binds and activates Notch; its signaling competence requires Mib1/Neuralized-dependent endocytosis. Participation in the Notch pathway is core. Supporting Evidence: PMID:23970418 A Serrate-Notch-Canoe complex mediates essential interactions between glia and neuroepithelial cells during Drosophila optic lobe development PMID:9202123 Ser and Dl encode transmembrane ligands for Notch, whereas fng encodes a pioneer protein. PMID:20176925 Endocytosis of the transmembrane ligands Delta (Dl) and Serrate (Ser) is required for the proper activation of Notch receptors. |
| GO:0007219 Notch signaling pathway | IMP PMID:28960177 Ubiquitylation-independent activation of Notch signalling by... | ACCEPT | Summary: Serrate is a Notch ligand and a core component of the Notch signaling pathway (IMP). Reason: Serrate binds and activates Notch; its signaling competence requires Mib1/Neuralized-dependent endocytosis. Participation in the Notch pathway is core. Supporting Evidence: PMID:28960177 Notch receptors are hetero-dimeric type 1 trans-membrane proteins, which are activated through ligands of the Delta/Serrate/Lag2 (DSL) protein family PMID:9202123 Ser and Dl encode transmembrane ligands for Notch, whereas fng encodes a pioneer protein. PMID:20176925 Endocytosis of the transmembrane ligands Delta (Dl) and Serrate (Ser) is required for the proper activation of Notch receptors. |
| GO:0007219 Notch signaling pathway | IMP PMID:8756291 The intracellular deletions of Delta and Serrate define domi... | ACCEPT | Summary: Serrate is a Notch ligand and a core component of the Notch signaling pathway (IMP). Reason: Serrate binds and activates Notch; its signaling competence requires Mib1/Neuralized-dependent endocytosis. Participation in the Notch pathway is core. Supporting Evidence: PMID:8756291 The intracellular deletions of Delta and Serrate define dominant negative forms of the Drosophila Notch ligands PMID:9202123 Ser and Dl encode transmembrane ligands for Notch, whereas fng encodes a pioneer protein. PMID:20176925 Endocytosis of the transmembrane ligands Delta (Dl) and Serrate (Ser) is required for the proper activation of Notch receptors. |
| GO:0007219 Notch signaling pathway | IMP PMID:9202123 Fringe modulates Notch-ligand interactions. | ACCEPT | Summary: Serrate is a Notch ligand and a core component of the Notch signaling pathway (IMP). Reason: Serrate binds and activates Notch; its signaling competence requires Mib1/Neuralized-dependent endocytosis. Participation in the Notch pathway is core. Supporting Evidence: PMID:9202123 Three genes, fringe (fng), Serrate (Ser) and Delta (Dl), are involved in the cellular interactions leading to Notch activation PMID:20176925 Endocytosis of the transmembrane ligands Delta (Dl) and Serrate (Ser) is required for the proper activation of Notch receptors. |
| GO:0007423 sensory organ development | IMP PMID:16093323 The interplay between DSL proteins and ubiquitin ligases in ... | KEEP AS NON CORE | Summary: Ser IMP annotation to 'sensory organ development'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. Supporting Evidence: PMID:16093323 Simultaneous absence of neuralized and mib1 completely abolishes Notch signaling in both aforementioned contexts, making it likely that ubiquitination is a prerequisite for Delta/Serrate signaling |
| GO:0007435 salivary gland morphogenesis | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Ser IEA annotation to 'salivary gland morphogenesis'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. |
| GO:0007435 salivary gland morphogenesis | IMP PMID:12798300 Specification of cell fates within the salivary gland primor... | KEEP AS NON CORE | Summary: Ser IMP annotation to 'salivary gland morphogenesis'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. Supporting Evidence: PMID:12798300 In conflict with this model, we find that three genes, dead ringer (dri), Serrate (Ser), and trh itself, are expressed in the duct independently of trh |
| GO:0007436 larval salivary gland morphogenesis | IMP PMID:12798300 Specification of cell fates within the salivary gland primor... | KEEP AS NON CORE | Summary: Ser IMP annotation to 'larval salivary gland morphogenesis'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. Supporting Evidence: PMID:12798300 In conflict with this model, we find that three genes, dead ringer (dri), Serrate (Ser), and trh itself, are expressed in the duct independently of trh |
| GO:0007476 imaginal disc-derived wing morphogenesis | IMP PMID:15829515 The ubiquitin ligase Drosophila Mind bomb promotes Notch sig... | KEEP AS NON CORE | Summary: Ser IMP annotation to 'imaginal disc-derived wing morphogenesis'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. Supporting Evidence: PMID:15829515 The ubiquitin ligase Drosophila Mind bomb promotes Notch signaling by regulating the localization and activity of Serrate and Delta |
| GO:0007480 imaginal disc-derived leg morphogenesis | IMP PMID:10357942 Composite signalling from Serrate and Delta establishes leg ... | KEEP AS NON CORE | Summary: Ser IMP annotation to 'imaginal disc-derived leg morphogenesis'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. Supporting Evidence: PMID:10357942 Composite signalling from Serrate and Delta establishes leg segments in Drosophila through Notch |
| GO:0008587 imaginal disc-derived wing margin morphogenesis | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Ser IEA annotation to 'imaginal disc-derived wing margin morphogenesis'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. |
| GO:0008587 imaginal disc-derived wing margin morphogenesis | IMP PMID:1657403 Specific EGF repeats of Notch mediate interactions with Delt... | KEEP AS NON CORE | Summary: Ser IMP annotation to 'imaginal disc-derived wing margin morphogenesis'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. Supporting Evidence: PMID:1657403 Specific EGF repeats of Notch mediate interactions with Delta and Serrate: implications for Notch as a multifunctional receptor |
| GO:0008587 imaginal disc-derived wing margin morphogenesis | IMP PMID:9202123 Fringe modulates Notch-ligand interactions. | KEEP AS NON CORE | Summary: Ser IMP annotation to 'imaginal disc-derived wing margin morphogenesis'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. Supporting Evidence: PMID:9202123 Three genes, fringe (fng), Serrate (Ser) and Delta (Dl), are involved in the cellular interactions leading to Notch activation |
| GO:0009986 cell surface | IDA PMID:20203305 Gene deletion screen for cardiomyopathy in adult Drosophila ... | ACCEPT | Summary: Serrate is a cell-surface transmembrane protein. Reason: Consistent with its role as a membrane-tethered ligand. Supporting Evidence: PMID:20203305 The membrane-spanning Notch (N) receptor is stimulated by the ligands Delta (Dl) or Serrate (Ser), which are present on the surface of neighboring cells PMID:1840519 Antibodies directed against part of the extracellular region confirm the localization of the protein in the membrane. |
| GO:0009986 cell surface | IEA GO_REF:0000117 | ACCEPT | Summary: Serrate is a cell-surface transmembrane protein. Reason: Consistent with its role as a membrane-tethered ligand. Supporting Evidence: PMID:1840519 Antibodies directed against part of the extracellular region confirm the localization of the protein in the membrane. |
| GO:0010008 endosome membrane | EXP PMID:20176925 Notch ligand activity is modulated by glycosphingolipid memb... | KEEP AS NON CORE | Summary: Serrate is endocytosed and found in vesicles, endosomes and multivesicular bodies. Reason: Mib1/Neuralized-dependent endocytosis is required for Serrate signaling competence and also mediates turnover. Endosomal localization is documented but regulatory; non-core. Supporting Evidence: PMID:20176925 Endocytosis of the transmembrane ligands Delta (Dl) and Serrate (Ser) is required for the proper activation of Notch receptors |
| GO:0010008 endosome membrane | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: Serrate is endocytosed and found in vesicles, endosomes and multivesicular bodies. Reason: Mib1/Neuralized-dependent endocytosis is required for Serrate signaling competence and also mediates turnover. Endosomal localization is documented but regulatory; non-core. Supporting Evidence: PMID:20176925 Endocytosis of the transmembrane ligands Delta (Dl) and Serrate (Ser) is required for the proper activation of Notch receptors. |
| GO:0014009 glial cell proliferation | IMP PMID:23970418 A Serrate-Notch-Canoe complex mediates essential interaction... | KEEP AS NON CORE | Summary: Ser IMP annotation to 'glial cell proliferation'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. Supporting Evidence: PMID:23970418 Ser is tissue-autonomously required in the glia where it activates Notch to regulate its proliferation, and non-autonomously in the neuroepithelium where Ser induces Notch signaling to avoid the premature activation of the EGFR-Ras pathway and hence of L'sc |
| GO:0016020 membrane | IEA GO_REF:0000002 | ACCEPT | Summary: Serrate is a cell-surface transmembrane protein. Reason: Consistent with its role as a membrane-tethered ligand. Supporting Evidence: PMID:1840519 Antibodies directed against part of the extracellular region confirm the localization of the protein in the membrane. |
| GO:0016324 apical plasma membrane | EXP PMID:15760269 Two distinct E3 ubiquitin ligases have complementary functio... | ACCEPT | Summary: Serrate is a single-pass type I transmembrane protein at the plasma membrane, enriched apically in epithelia (EXP). Reason: Surface presentation is required for trans-activation of Notch on neighbouring cells. Supporting Evidence: PMID:15760269 Signaling by the Notch ligands Delta (Dl) and Serrate (Ser) regulates a wide variety of essential cell-fate decisions during animal development PMID:1840519 Antibodies directed against part of the extracellular region confirm the localization of the protein in the membrane. |
| GO:0016324 apical plasma membrane | EXP PMID:15829515 The ubiquitin ligase Drosophila Mind bomb promotes Notch sig... | ACCEPT | Summary: Serrate is a single-pass type I transmembrane protein at the plasma membrane, enriched apically in epithelia (EXP). Reason: Surface presentation is required for trans-activation of Notch on neighbouring cells. Supporting Evidence: PMID:15829515 The ubiquitin ligase Drosophila Mind bomb promotes Notch signaling by regulating the localization and activity of Serrate and Delta PMID:1840519 Antibodies directed against part of the extracellular region confirm the localization of the protein in the membrane. |
| GO:0016324 apical plasma membrane | EXP PMID:1840519 The Drosophila gene Serrate encodes an EGF-like transmembran... | ACCEPT | Summary: Serrate is a single-pass type I transmembrane protein at the plasma membrane, enriched apically in epithelia (EXP). Reason: Surface presentation is required for trans-activation of Notch on neighbouring cells. Supporting Evidence: PMID:1840519 The Drosophila gene Serrate encodes an EGF-like transmembrane protein with a complex expression pattern in embryos and wing discs |
| GO:0016324 apical plasma membrane | EXP PMID:20176925 Notch ligand activity is modulated by glycosphingolipid memb... | ACCEPT | Summary: Serrate is a single-pass type I transmembrane protein at the plasma membrane, enriched apically in epithelia (EXP). Reason: Surface presentation is required for trans-activation of Notch on neighbouring cells. Supporting Evidence: PMID:20176925 Endocytosis of the transmembrane ligands Delta (Dl) and Serrate (Ser) is required for the proper activation of Notch receptors PMID:1840519 Antibodies directed against part of the extracellular region confirm the localization of the protein in the membrane. |
| GO:0016324 apical plasma membrane | IDA PMID:17006545 Role of conserved intracellular motifs in Serrate signalling... | ACCEPT | Summary: Serrate is a single-pass type I transmembrane protein at the plasma membrane, enriched apically in epithelia (IDA). Reason: Surface presentation is required for trans-activation of Notch on neighbouring cells. Supporting Evidence: PMID:17006545 Serrate) are transmembrane proteins whose signalling competence is regulated by the endocytosis-promoting E3 ubiquitin ligases, Mindbomb1 and Neuralized PMID:1840519 Antibodies directed against part of the extracellular region confirm the localization of the protein in the membrane. |
| GO:0016324 apical plasma membrane | IEA GO_REF:0000120 | ACCEPT | Summary: Serrate is a single-pass type I transmembrane protein at the plasma membrane, enriched apically in epithelia (IEA). Reason: Surface presentation is required for trans-activation of Notch on neighbouring cells. Supporting Evidence: PMID:1840519 Antibodies directed against part of the extracellular region confirm the localization of the protein in the membrane. |
| GO:0016330 second mitotic wave involved in compound eye morphogenesis | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Ser IEA annotation to 'second mitotic wave involved in compound eye morphogenesis'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. |
| GO:0016330 second mitotic wave involved in compound eye morphogenesis | IMP PMID:23970418 A Serrate-Notch-Canoe complex mediates essential interaction... | KEEP AS NON CORE | Summary: Ser IMP annotation to 'second mitotic wave involved in compound eye morphogenesis'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. Supporting Evidence: PMID:23970418 Ser is tissue-autonomously required in the glia where it activates Notch to regulate its proliferation, and non-autonomously in the neuroepithelium where Ser induces Notch signaling to avoid the premature activation of the EGFR-Ras pathway and hence of L'sc |
| GO:0030424 axon | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: Serrate protein detected on axons/neuronal projections. Reason: Reported localization (UniProt cites PMID:1840519); Serrate also signals in glia-neuron interactions. Not a core site of function. |
| GO:0030673 axolemma | IDA PMID:1840519 The Drosophila gene Serrate encodes an EGF-like transmembran... | KEEP AS NON CORE | Summary: Serrate protein detected on axons/neuronal projections. Reason: Reported localization (UniProt cites PMID:1840519); Serrate also signals in glia-neuron interactions. Not a core site of function. Supporting Evidence: PMID:1840519 The Drosophila gene Serrate encodes an EGF-like transmembrane protein with a complex expression pattern in embryos and wing discs |
| GO:0030718 germ-line stem cell population maintenance | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Ser IEA annotation to 'germ-line stem cell population maintenance'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. |
| GO:0030718 germ-line stem cell population maintenance | IGI PMID:17070683 Stem cells signal to the niche through the Notch pathway in ... | KEEP AS NON CORE | Summary: Ser IGI annotation to 'germ-line stem cell population maintenance'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. Supporting Evidence: PMID:17070683 Conversely, when GCSs do not produce functional Notch ligands, Delta and Serrate, the TGF-beta pathway is not activated in the GSCs, and they differentiate and subsequently leave the niche |
| GO:0031982 vesicle | EXP PMID:15829515 The ubiquitin ligase Drosophila Mind bomb promotes Notch sig... | KEEP AS NON CORE | Summary: Serrate is endocytosed and found in vesicles, endosomes and multivesicular bodies. Reason: Mib1/Neuralized-dependent endocytosis is required for Serrate signaling competence and also mediates turnover. Endosomal localization is documented but regulatory; non-core. Supporting Evidence: PMID:15829515 We find that D-mib uses its ubiquitin ligase activity to promote DSL ligand activity, an activity that is correlated with its ability to induce the endocytosis and degradation of both Delta and Serrate (see also Le Borgne et al., 2005) PMID:20176925 Endocytosis of the transmembrane ligands Delta (Dl) and Serrate (Ser) is required for the proper activation of Notch receptors. |
| GO:0031982 vesicle | EXP PMID:1840519 The Drosophila gene Serrate encodes an EGF-like transmembran... | KEEP AS NON CORE | Summary: Serrate is endocytosed and found in vesicles, endosomes and multivesicular bodies. Reason: Mib1/Neuralized-dependent endocytosis is required for Serrate signaling competence and also mediates turnover. Endosomal localization is documented but regulatory; non-core. Supporting Evidence: PMID:1840519 The Drosophila gene Serrate encodes an EGF-like transmembrane protein with a complex expression pattern in embryos and wing discs PMID:20176925 Endocytosis of the transmembrane ligands Delta (Dl) and Serrate (Ser) is required for the proper activation of Notch receptors. |
| GO:0031982 vesicle | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: Serrate is endocytosed and found in vesicles, endosomes and multivesicular bodies. Reason: Mib1/Neuralized-dependent endocytosis is required for Serrate signaling competence and also mediates turnover. Endosomal localization is documented but regulatory; non-core. Supporting Evidence: PMID:20176925 Endocytosis of the transmembrane ligands Delta (Dl) and Serrate (Ser) is required for the proper activation of Notch receptors. |
| GO:0035017 cuticle pattern formation | IMP PMID:16125166 Serrate-Notch signaling defines the scope of the initial den... | KEEP AS NON CORE | Summary: Ser IMP annotation to 'cuticle pattern formation'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. Supporting Evidence: PMID:16125166 Serrate-Notch signaling defines the scope of the initial denticle field by modulating EGFR activation |
| GO:0035167 larval lymph gland hemopoiesis | IEP PMID:12569125 A Serrate-expressing signaling center controls Drosophila he... | KEEP AS NON CORE | Summary: Serrate is expressed in a lymph gland signaling center (posterior signaling center-like cells) that controls hematopoiesis (IEP). Reason: The expression pattern plus functional data from the same study (crystal cell specification) support involvement of Serrate-Notch signaling in lymph gland hematopoiesis; non-core developmental context. Supporting Evidence: PMID:12569125 The expression of Serrate marks a discrete cluster of cells in the lymph gland, a signaling center, with functional similarities to stromal signaling in mammalian hematopoiesis |
| GO:0035204 negative regulation of lamellocyte differentiation | IMP PMID:24318532 An unexpected link between notch signaling and ROS in restri... | KEEP AS NON CORE | Summary: Ser IMP annotation to 'negative regulation of lamellocyte differentiation'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. Supporting Evidence: PMID:24318532 In larval stages, Notch signaling regulates commitment to crystal cell lineage ( Duvic et al. 2002 ; Lebestky et al. 2003 ); in procrystal cells, the Notch receptor is activated by Serrate (Ser), expressed in the niche cells |
| GO:0035214 eye-antennal disc development | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Ser IEA annotation to 'eye-antennal disc development'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. |
| GO:0035214 eye-antennal disc development | IMP PMID:17090721 Lobe and Serrate are required for cell survival during early... | KEEP AS NON CORE | Summary: Ser IMP annotation to 'eye-antennal disc development'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. Supporting Evidence: PMID:17090721 Here, we show that Lobe (L) and the Notch (N) ligand Serrate (Ser), which are both involved in ventral eye growth, are required for cell survival in the early eye disc |
| GO:0036011 imaginal disc-derived leg segmentation | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Ser IEA annotation to 'imaginal disc-derived leg segmentation'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. |
| GO:0036011 imaginal disc-derived leg segmentation | IMP PMID:10357895 Notch-mediated segmentation and growth control of the Drosop... | KEEP AS NON CORE | Summary: Ser IMP annotation to 'imaginal disc-derived leg segmentation'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. Supporting Evidence: PMID:10357895 This segmentation process requires the participation of the Notch ligands, Serrate and Delta, as well as Fringe |
| GO:0042063 gliogenesis | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Ser IEA annotation to 'gliogenesis'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. |
| GO:0042688 crystal cell differentiation | IMP PMID:12445385 Notch signaling controls lineage specification during Drosop... | KEEP AS NON CORE | Summary: Ser IMP annotation to 'crystal cell differentiation'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. Supporting Evidence: PMID:12445385 We demonstrate that, in this process, Serrate, not Delta, is the Notch ligand |
| GO:0042688 crystal cell differentiation | IMP PMID:12569125 A Serrate-expressing signaling center controls Drosophila he... | KEEP AS NON CORE | Summary: Ser IMP annotation to 'crystal cell differentiation'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. Supporting Evidence: PMID:12569125 We also find that a signaling center expressing the ligand Serrate is important for differentiation and proliferation of larval hemocytes in Drosophila . Results and Discussion Notch and Su(H) are required for hemocyte proliferation and development Morphological and molecular differentiation of hemocytes can be monitored in the larval lymph gland ( Shrestha and Gateff 1982 ) |
| GO:0043005 neuron projection | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Serrate protein detected on axons/neuronal projections. Reason: Reported localization (UniProt cites PMID:1840519); Serrate also signals in glia-neuron interactions. Not a core site of function. |
| GO:0043208 glycosphingolipid binding | IDA PMID:20176925 Notch ligand activity is modulated by glycosphingolipid memb... | KEEP AS NON CORE | Summary: The Serrate extracellular domain binds glycosphingolipids in vitro via a conserved GSL-binding motif. Reason: Direct biochemical evidence; the interaction modulates ligand endocytosis and signaling activity. Regulatory, non-core. Supporting Evidence: PMID:20176925 Our genetic and biochemical analysis of Ξ±4GT1 function indicates that specific changes in glycosphingolipid (GSL) composition can rescue the defects in Dl and Ser trafficking and signaling seen upon inhibition of mib1 activity, thereby establishing a new functional link between GSLs and Notch signaling |
| GO:0043208 glycosphingolipid binding | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: The Serrate extracellular domain binds glycosphingolipids in vitro via a conserved GSL-binding motif. Reason: Direct biochemical evidence; the interaction modulates ligand endocytosis and signaling activity. Regulatory, non-core. |
| GO:0045179 apical cortex | IDA PMID:15760269 Two distinct E3 ubiquitin ligases have complementary functio... | KEEP AS NON CORE | Summary: Serrate detected at the apical cortex of wing imaginal cells. Reason: Correct but less informative than apical plasma membrane; non-core. Supporting Evidence: PMID:15760269 We therefore conclude that D-mib and neur have distinct and complementary functions in Drosophila.D-mib Co-Localizes with Dl and Ser at the Apical CortexWe next studied the subcellular localization of D-mib (Figure 3) |
| GO:0045179 apical cortex | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Serrate detected at the apical cortex of wing imaginal cells. Reason: Correct but less informative than apical plasma membrane; non-core. |
| GO:0046331 lateral inhibition | IEA GO_REF:0000117 | ACCEPT | Summary: Serrate participates, partly redundantly with Delta, in Notch-mediated lateral inhibition. Reason: As a DSL ligand, Serrate directly mediates Notch activation during lateral inhibition in sensory organ development (redundant with Delta). |
| GO:0046331 lateral inhibition | IMP PMID:16093323 The interplay between DSL proteins and ubiquitin ligases in ... | ACCEPT | Summary: Serrate participates, partly redundantly with Delta, in Notch-mediated lateral inhibition. Reason: As a DSL ligand, Serrate directly mediates Notch activation during lateral inhibition in sensory organ development (redundant with Delta). Supporting Evidence: PMID:16093323 Of the two Notch ligands Delta and Serrate, only the former was thought to participate in this process |
| GO:0048018 receptor ligand activity | IDA PMID:20203305 Gene deletion screen for cardiomyopathy in adult Drosophila ... | ACCEPT | Summary: Serrate acts as a ligand that activates the Notch receptor. Reason: Serrate trans-activates Notch (e.g. dorsal-to-ventral signaling at the wing D/V boundary, crystal cell specification). Core activity. Supporting Evidence: PMID:20203305 Importantly, Wry lacks a DSL (Delta-Serrate-Lag) domain that is common feature to the other Drosophila Notch ligands PMID:9202123 Ser and Dl encode transmembrane ligands for Notch, whereas fng encodes a pioneer protein. PMID:12569125 Serrate signaling through the Notch pathway is critical in the regulation of Lz expression and the specification of crystal cell precursors, thus providing a key distinction between the two lineages. file:DROME/Ser/Ser-deep-research-falcon.md When Serrate and Notch are co-expressed on the same cell surface, their cis-interaction sequesters Notch |
| GO:0048018 receptor ligand activity | IEA GO_REF:0000117 | ACCEPT | Summary: Serrate acts as a ligand that activates the Notch receptor. Reason: Serrate trans-activates Notch (e.g. dorsal-to-ventral signaling at the wing D/V boundary, crystal cell specification). Core activity. Supporting Evidence: PMID:9202123 Ser and Dl encode transmembrane ligands for Notch, whereas fng encodes a pioneer protein. PMID:12569125 Serrate signaling through the Notch pathway is critical in the regulation of Lz expression and the specification of crystal cell precursors, thus providing a key distinction between the two lineages. file:DROME/Ser/Ser-deep-research-falcon.md When Serrate and Notch are co-expressed on the same cell surface, their cis-interaction sequesters Notch |
| GO:0048100 wing disc anterior/posterior pattern formation | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Ser IEA annotation to 'wing disc anterior/posterior pattern formation'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. |
| GO:0048100 wing disc anterior/posterior pattern formation | IMP PMID:23152840 Sequential Notch signalling at the boundary of fringe expres... | KEEP AS NON CORE | Summary: Ser IMP annotation to 'wing disc anterior/posterior pattern formation'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. Supporting Evidence: PMID:23152840 It appears that mutual signalling from dorsal to ventral and ventral to dorsal cells by the Notch ligands Serrate (Ser) and Delta (Dl) respectively establishes a symmetric domain of Wg that straddles the D/V boundary |
| GO:0048468 cell development | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Ser IEA annotation to 'cell development'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. |
| GO:0048749 compound eye development | IMP PMID:17090721 Lobe and Serrate are required for cell survival during early... | KEEP AS NON CORE | Summary: Ser IMP annotation to 'compound eye development'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. Supporting Evidence: PMID:17090721 Here, we show that Lobe (L) and the Notch (N) ligand Serrate (Ser), which are both involved in ventral eye growth, are required for cell survival in the early eye disc |
| GO:0051093 negative regulation of developmental process | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Ser IEA annotation to 'negative regulation of developmental process'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. |
| GO:0051239 regulation of multicellular organismal process | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Ser IEA annotation to 'regulation of multicellular organismal process'. Serrate is a Notch ligand, and perturbing Serrate alters this developmental process through loss or misregulation of Notch signaling. Reason: Serrate (Jagged ortholog) is a transmembrane DSL ligand of Notch; its requirement in this context reflects Serrate-Notch signaling there. The annotation is biologically sound but records a downstream developmental outcome rather than the core ligand activity; kept as non-core. |
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Experiment: Compare endogenous Serrate and Delta cis-inhibition strength at the wing D/V boundary using ligand alleles specifically defective in trans-activation (Mib1/Neur-binding motif mutants).
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