Tango5 is an ER-associated multipass membrane protein of the VMP1/DedA-related group. Drosophila depletion experiments implicate it in constitutive secretion and Golgi organization. VMP1 homologs participate in membrane lipid handling and autophagy, but the precise transported lipids and catalytic or transport mechanism of the fly product have not been directly resolved.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0003674 molecular_function | ND GO_REF:0000015 | ACCEPT | Summary: A substrate-resolved molecular activity remains unassigned in fly. Reason: The ND annotation is an explicit absence of a specific GO molecular-function assignment, not evidence of inactivity. VMP1-family scramblase work supplies a mechanistic lead, while the fly experiments establish secretion and organellar effects rather than a purified lipid-scrambling reaction. Supporting Evidence: PMID:16452979 We performed a genome-wide RNA-mediated interference screen in a Drosophila cell line to identify genes required for constitutive protein secretion. |
| GO:0005774 vacuolar membrane | IEA GO_REF:0000044 | UNDECIDED | Summary: Vacuolar-membrane localization is not established by the VMP1 name. Reason: The direct fly localization is ER-associated. Membrane topology supports integration but does not itself place Tango5 in a vacuolar membrane, so the name-derived electronic location is unresolved. Supporting Evidence: file:genes/DROME/Tango5/Tango5-flybase.txt located_in endoplasmic reticulum inferred from direct assay file:DROME/Tango5/Tango5-uniprot.txt FT TRANSMEM 69..86 |
| GO:0005783 endoplasmic reticulum | IDA PMID:16452979 Functional genomics reveals genes involved in protein secret... | ACCEPT | Summary: Tango5 localizes to the endoplasmic reticulum. Reason: FlyBase traces the target localization to the direct-assay results of the Drosophila secretion screen. This target-specific observation supports the ER assignment independently of ARBA. Supporting Evidence: file:genes/DROME/Tango5/Tango5-flybase.txt located_in endoplasmic reticulum inferred from direct assay PMID:16452979 We performed a genome-wide RNA-mediated interference screen in a Drosophila cell line to identify genes required for constitutive protein secretion. |
| GO:0005783 endoplasmic reticulum | IEA GO_REF:0000044 | ACCEPT | Summary: Tango5 localizes to the endoplasmic reticulum. Reason: FlyBase traces the target localization to the direct-assay results of the Drosophila secretion screen. This target-specific observation supports the ER assignment independently of ARBA. Supporting Evidence: file:genes/DROME/Tango5/Tango5-flybase.txt located_in endoplasmic reticulum inferred from direct assay PMID:16452979 We performed a genome-wide RNA-mediated interference screen in a Drosophila cell line to identify genes required for constitutive protein secretion. |
| GO:0007030 Golgi organization | IMP PMID:16452979 Functional genomics reveals genes involved in protein secret... | ACCEPT | Summary: Tango5 contributes to Golgi organization through the secretory membrane system. Reason: The target is among the experimental secretion-screen genes whose depletion affects Golgi organization. Its ER residence is compatible with an upstream effect on Golgi membrane maintenance, without making it a Golgi enzyme. Supporting Evidence: PMID:16452979 We performed a genome-wide RNA-mediated interference screen in a Drosophila cell line to identify genes required for constitutive protein secretion. file:genes/DROME/Tango5/Tango5-flybase.txt located_in endoplasmic reticulum inferred from direct assay |
| GO:0009306 protein secretion | IMP PMID:16452979 Functional genomics reveals genes involved in protein secret... | ACCEPT | Summary: Tango5 contributes to constitutive protein secretion. Reason: The Drosophila loss-of-function screen and target-specific FlyBase curation identify a secretion requirement. This establishes process participation while leaving the exact lipid-handling mechanism in fly open. Supporting Evidence: PMID:16452979 We performed a genome-wide RNA-mediated interference screen in a Drosophila cell line to identify genes required for constitutive protein secretion. |
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