Vps50

Summary: SNARE binding inferred from human VPS50/syndetin.

Reason: Human EARP binds syntaxin 6 and cognate SNAREs, which is part of its tethering mechanism; this is plausible for fly Vps50 but untested, and is better represented by the complex's tethering activity. IBA donor human VPS50 (Q96JG6).

Summary: Vps50 is part of the Vps54-free form of GARP described as GARPII, which corresponds to EARP.

Reason: The cited paper found CG4996 co-purifying with Vps51-53 in place of Vps54 and named the resulting complex GARPII. This complex was subsequently defined in mammals as EARP, which has its own GO term (GO:1990745); GARP complex (GO:0000938) refers to the Vps54-containing complex. The correct location for Vps50 is EARP complex.

Proposed replacements: EARP complex

Summary: Vps50 is part of the Vps54-free form of GARP described as GARPII, which corresponds to EARP.

Reason: The cited paper found CG4996 co-purifying with Vps51-53 in place of Vps54 and named the resulting complex GARPII. This complex was subsequently defined in mammals as EARP, which has its own GO term (GO:1990745); GARP complex (GO:0000938) refers to the Vps54-containing complex. The correct location for Vps50 is EARP complex.

Proposed replacements: EARP complex

Summary: Root molecular function ND annotation: when it was made, no molecular function had been curated for this gene product from literature.

Reason: ACCEPT under the ND acceptance rule (projects/NO_DATA.md). The evidence this review cites for the molecular function is inferred (from orthologs, family membership or sequence features) or comes from other species; none of it is literature on this gene product in this species. This was judged from the review's own content during the NO_DATA re-action pass, not from a new literature search. Earlier action: MODIFY. Re-checked 2026-10-31: the tethering papers cited in core_functions are on human EARP and a Rab-effector map (PMID:25799061, PMID:25453831). The term the earlier MODIFY proposed, vesicle membrane tethering activity, is kept as its own NEW row. Possibly stale: the 2026-11-06 ND citation pass found literature on this gene in this species that may identify a molecular function; see Vps50-notes.md, section "ND literature-search citations (2026-11-06)". A curator should check it before this ACCEPT stands.

Summary: GFP-Vps50 colocalizes with Rab4 on endosomes in S2 cells.

Reason: Direct localization of the fly protein with the endosomal Rab4 and Hrs-labelled compartment.

Summary: Cytosolic pool of a peripheral membrane complex.

Reason: Tethering-complex subunits cycle between membrane-bound and cytosolic pools; the functional location is the endosome membrane, so cytosol is non-core. Inferred from the (over-annotated) endosome-to-Golgi process annotation (GO_REF:0000108).

Summary: EARP, defined by Vps50/syndetin, acts in endocytic recycling.

Reason: Depletion of syndetin, the human ortholog of Vps50, delays transferrin recycling to the cell surface; endocytic recycling is the core process of EARP. The fly protein binds the recycling-associated Rab4 and localizes with it on endosomes. IBA/ISS donors human VPS50 (Q96JG6) and mouse Vps50 (MGI:1920538).

Summary: EARP, defined by Vps50/syndetin, acts in endocytic recycling.

Reason: Depletion of syndetin, the human ortholog of Vps50, delays transferrin recycling to the cell surface; endocytic recycling is the core process of EARP. The fly protein binds the recycling-associated Rab4 and localizes with it on endosomes. IBA/ISS donors human VPS50 (Q96JG6) and mouse Vps50 (MGI:1920538).

Summary: EARP, defined by Vps50/syndetin, acts in endocytic recycling.

Reason: Depletion of syndetin, the human ortholog of Vps50, delays transferrin recycling to the cell surface; endocytic recycling is the core process of EARP. The fly protein binds the recycling-associated Rab4 and localizes with it on endosomes. IBA/ISS donors human VPS50 (Q96JG6) and mouse Vps50 (MGI:1920538).

Summary: EARP, defined by Vps50/syndetin, acts in endocytic recycling.

Reason: Depletion of syndetin, the human ortholog of Vps50, delays transferrin recycling to the cell surface; endocytic recycling is the core process of EARP. The fly protein binds the recycling-associated Rab4 and localizes with it on endosomes. IBA/ISS donors human VPS50 (Q96JG6) and mouse Vps50 (MGI:1920538).

Summary: Endosome-to-Golgi retrograde transport is the function of GARP (Vps54), not of Vps50-containing EARP.

Reason: Depletion of syndetin, the human Vps50 ortholog, did not affect retrograde transport of Shiga toxin B subunit to the Golgi or TGN46 localization; the retrograde process belongs to GARP, which contains Vps54/scat instead of Vps50. The IEA (InterPro IPR040047) and NAS rows transfer a GARP property to the EARP-specific subunit.

Summary: Endosome-to-Golgi retrograde transport is the function of GARP (Vps54), not of Vps50-containing EARP.

Reason: Depletion of syndetin, the human Vps50 ortholog, did not affect retrograde transport of Shiga toxin B subunit to the Golgi or TGN46 localization; the retrograde process belongs to GARP, which contains Vps54/scat instead of Vps50. The IEA (InterPro IPR040047) and NAS rows transfer a GARP property to the EARP-specific subunit.

Summary: Vps50 is the defining subunit of the EARP complex.

Reason: Fly Vps50/CG4996 associates with the Vps51-Vps53 core in place of Vps54, as mammalian syndetin does in EARP. IBA donor human VPS50 (Q96JG6); IEA from InterPro IPR040047.

Summary: Vps50 is the defining subunit of the EARP complex.

Reason: Fly Vps50/CG4996 associates with the Vps51-Vps53 core in place of Vps54, as mammalian syndetin does in EARP. IBA donor human VPS50 (Q96JG6); IEA from InterPro IPR040047.

Summary: Inferred vesicle membrane tethering activity for this gene product, proposed during the NO_DATA re-action pass.

Reason: Vesicle membrane tethering activity is inferred for this gene product (ISS), not shown for it experimentally; the ND row for the aspect therefore stays (projects/NO_DATA.md). Vps50 contributes, as the defining subunit of EARP, to the vesicle-tethering activity of the complex (contributes_to).