dati

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0005634 nucleus	IEA GO_REF:0000120	ACCEPT	Summary: DATI immunostaining overlaps a nuclear fluorescent reporter in identified neurons, consistent with its zinc-finger transcription-regulatory role. This primary localization evidence supports the nuclear assignment independently of electronic inference. Reason: DATI immunostaining overlaps a nuclear fluorescent reporter in identified neurons, consistent with its zinc-finger transcription-regulatory role. This primary localization evidence supports the nuclear assignment independently of electronic inference. Supporting Evidence: PMID:25291190 DATI protein stained in magenta, Cha-Gal4 UAS-mCherry.NLS in green, and the overlap between the two in light magenta or white PMID:25291190 dati encodes a zinc finger transcription factor closely related to rotund (rn) and squeeze (sqz) with homologs in several species, including humans
GO:0008344 adult locomotory behavior	IMP PMID:25291190 The KRÜPPEL-like transcription factor DATILÓGRAFO is require...	KEEP AS NON CORE	Summary: Mutant and cell-specific RNAi experiments establish a locomotor phenotype, separable from courtship receptivity. Retain behavior as a downstream consequence of neuronal specification and function rather than a molecular activity. Reason: Mutant and cell-specific RNAi experiments establish a locomotor phenotype, separable from courtship receptivity. Retain behavior as a downstream consequence of neuronal specification and function rather than a molecular activity. Supporting Evidence: PMID:25291190 Courtship acceptance and locomotor deficits in dati mutant females are separable phenotypes.
GO:0016319 mushroom body development	IMP PMID:25291190 The KRÜPPEL-like transcription factor DATILÓGRAFO is require...	KEEP AS NON CORE	Summary: The full primary paper demonstrates malformed mushroom-body gamma lobes in dati mutants. This developmental role is retained as noncore; the study explicitly separates the gamma-lobe phenotype from the cells responsible for courtship acceptance. Reason: The full primary paper demonstrates malformed mushroom-body gamma lobes in dati mutants. This developmental role is retained as noncore; the study explicitly separates the gamma-lobe phenotype from the cells responsible for courtship acceptance. Supporting Evidence: PMID:25291190 although the loss of dati disrupts the gamma lobe neuropile, the focus of dati-mediated courtship acceptance lies elsewhere in the brain.
GO:0031175 neuron projection development	IMP PMID:25291190 The KRÜPPEL-like transcription factor DATILÓGRAFO is require...	ACCEPT	Summary: Dati is required for normal cholinergic projection-neuron morphology and innervation of olfactory regions. This is a central developmental role of the neuronal regulator, supported by loss-of-function and cell-specific analyses. Reason: Dati is required for normal cholinergic projection-neuron morphology and innervation of olfactory regions. This is a central developmental role of the neuronal regulator, supported by loss-of-function and cell-specific analyses. Supporting Evidence: PMID:25291190 Homozygosity for dati1 causes loss of cholinergic projection neurons in the antennal lobe. PMID:25291190 Together, these results show that dati is required in postmitotic neurons as well as in the precursors of these cells.
GO:0045925 positive regulation of female receptivity	IMP PMID:25291190 The KRÜPPEL-like transcription factor DATILÓGRAFO is require...	KEEP AS NON CORE	Summary: Dati function in selected cholinergic neurons is required for female courtship acceptance. Retain the well-supported behavioral consequence as noncore relative to neuronal specification; it is not reducible to locomotor impairment. Reason: Dati function in selected cholinergic neurons is required for female courtship acceptance. Retain the well-supported behavioral consequence as noncore relative to neuronal specification; it is not reducible to locomotor impairment. Supporting Evidence: PMID:25291190 Expression of dati-RNAi specifically in cholinergic neurons causes the female rejection behavior. PMID:25291190 Courtship acceptance and locomotor deficits in dati mutant females are separable phenotypes.
GO:0048699 generation of neurons	IMP PMID:25291190 The KRÜPPEL-like transcription factor DATILÓGRAFO is require...	ACCEPT	Summary: The primary study identifies defects in neuronal subtype specification and precursor/postmitotic requirements. This supports participation in neurogenesis, without asserting that Dati specifies all cholinergic identity or that its loss merely kills every affected neuron. Reason: The primary study identifies defects in neuronal subtype specification and precursor/postmitotic requirements. This supports participation in neurogenesis, without asserting that Dati specifies all cholinergic identity or that its loss merely kills every affected neuron. Supporting Evidence: PMID:25291190 Together, these results show that dati is required in postmitotic neurons as well as in the precursors of these cells. PMID:25291190 Homozygosity for dati1 causes loss of cholinergic projection neurons in the antennal lobe.

GO Term

Evidence

Action

Reason

IEA
GO_REF:0000120

Summary: DATI immunostaining overlaps a nuclear fluorescent reporter in identified neurons, consistent with its zinc-finger transcription-regulatory role. This primary localization evidence supports the nuclear assignment independently of electronic inference.

Reason: DATI immunostaining overlaps a nuclear fluorescent reporter in identified neurons, consistent with its zinc-finger transcription-regulatory role. This primary localization evidence supports the nuclear assignment independently of electronic inference.

Supporting Evidence:

PMID:25291190

DATI protein stained in magenta, Cha-Gal4 UAS-mCherry.NLS in green, and the overlap between the two in light magenta or white

PMID:25291190

dati encodes a zinc finger transcription factor closely related to rotund (rn) and squeeze (sqz) with homologs in several species, including humans

GO:0008344 adult locomotory behavior

IMP
PMID:25291190
The KRÜPPEL-like transcription factor DATILÓGRAFO is require...

KEEP AS NON CORE

Summary: Mutant and cell-specific RNAi experiments establish a locomotor phenotype, separable from courtship receptivity. Retain behavior as a downstream consequence of neuronal specification and function rather than a molecular activity.

Reason: Mutant and cell-specific RNAi experiments establish a locomotor phenotype, separable from courtship receptivity. Retain behavior as a downstream consequence of neuronal specification and function rather than a molecular activity.

Supporting Evidence:

PMID:25291190

Courtship acceptance and locomotor deficits in dati mutant females are separable phenotypes.

GO:0016319 mushroom body development

IMP
PMID:25291190
The KRÜPPEL-like transcription factor DATILÓGRAFO is require...

KEEP AS NON CORE

Summary: The full primary paper demonstrates malformed mushroom-body gamma lobes in dati mutants. This developmental role is retained as noncore; the study explicitly separates the gamma-lobe phenotype from the cells responsible for courtship acceptance.

Reason: The full primary paper demonstrates malformed mushroom-body gamma lobes in dati mutants. This developmental role is retained as noncore; the study explicitly separates the gamma-lobe phenotype from the cells responsible for courtship acceptance.

Supporting Evidence:

PMID:25291190

although the loss of dati disrupts the gamma lobe neuropile, the focus of dati-mediated courtship acceptance lies elsewhere in the brain.

GO:0031175 neuron projection development

IMP
PMID:25291190
The KRÜPPEL-like transcription factor DATILÓGRAFO is require...

Summary: Dati is required for normal cholinergic projection-neuron morphology and innervation of olfactory regions. This is a central developmental role of the neuronal regulator, supported by loss-of-function and cell-specific analyses.

Reason: Dati is required for normal cholinergic projection-neuron morphology and innervation of olfactory regions. This is a central developmental role of the neuronal regulator, supported by loss-of-function and cell-specific analyses.

Supporting Evidence:

PMID:25291190

Homozygosity for dati1 causes loss of cholinergic projection neurons in the antennal lobe.

PMID:25291190

Together, these results show that dati is required in postmitotic neurons as well as in the precursors of these cells.

GO:0045925 positive regulation of female receptivity

IMP
PMID:25291190
The KRÜPPEL-like transcription factor DATILÓGRAFO is require...

KEEP AS NON CORE

Summary: Dati function in selected cholinergic neurons is required for female courtship acceptance. Retain the well-supported behavioral consequence as noncore relative to neuronal specification; it is not reducible to locomotor impairment.

Reason: Dati function in selected cholinergic neurons is required for female courtship acceptance. Retain the well-supported behavioral consequence as noncore relative to neuronal specification; it is not reducible to locomotor impairment.

Supporting Evidence:

PMID:25291190

Expression of dati-RNAi specifically in cholinergic neurons causes the female rejection behavior.

PMID:25291190

Courtship acceptance and locomotor deficits in dati mutant females are separable phenotypes.

GO:0048699 generation of neurons

IMP
PMID:25291190
The KRÜPPEL-like transcription factor DATILÓGRAFO is require...

Summary: The primary study identifies defects in neuronal subtype specification and precursor/postmitotic requirements. This supports participation in neurogenesis, without asserting that Dati specifies all cholinergic identity or that its loss merely kills every affected neuron.

Reason: The primary study identifies defects in neuronal subtype specification and precursor/postmitotic requirements. This supports participation in neurogenesis, without asserting that Dati specifies all cholinergic identity or that its loss merely kills every affected neuron.

Supporting Evidence:

PMID:25291190

Together, these results show that dati is required in postmitotic neurons as well as in the precursors of these cells.

PMID:25291190

Homozygosity for dati1 causes loss of cholinergic projection neurons in the antennal lobe.

Core Functions

Acts as a zinc-finger transcriptional regulator supporting neuronal subtype specification and projection development; DNA-binding mechanism is inferred from the characterized family.

Molecular Function:

DNA-binding transcription factor activity

Directly Involved In:

neuron projection development generation of neurons

Cellular Locations:

nucleus

Supporting Evidence:

PMID:25291190
dati encodes a zinc finger transcription factor closely related to rotund (rn) and squeeze (sqz) with homologs in several species, including humans
PMID:25291190
Here we described DATI, a zinc finger transcription factor related to the Drosophila Rotund and Squeeze and the vertebrate ZNF384
PMID:11867614
Zinc fingers 2, 3, and 6 mediated association with the homopolymeric (dA.dT) COL1A1/MMP DNA consensus element.
PMID:25291190
Together, these results show that dati is required in postmitotic neurons as well as in the precursors of these cells.

External Prediction Reviews

These computational predictions are reviewed separately from the GOA annotation set used for this review. The assessments below are from this project and do not constitute official GO annotations or endorsement by GO/UniProt. They are not included in the existing annotation review above.

ProtNLM2 External predictions

View prediction review YAML · dati-protnlm-predictions-review.yaml · Review status: COMPLETE

The known transcription-regulatory DNA-binding role is supported; specific activating activity remains unresolved.

Source documents: genes/DROME/dati/dati-predictions-source.json · genes/DROME/dati/dati-uniprot-source.json

Review score: 2 = concordant with evidence; 1 = uncertain; 0 = discordant with evidence. This is an assessment score, not a model probability.

GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific GO_MF

UNC — Uncertain Review score: 1/2

Prediction method: ProtNLM2 · Version: UniProt API snapshot 2026-09-08 · file:DROME/dati/dati-predictions-source.json

Review rationale: A transcription-regulatory role is well supported, but the specific claim of transcriptional activation remains unresolved. The target primary study establishes neuronal specification and identifies its zinc-finger family without measuring activation of a defined target promoter. Related Nmp4/ZNF384 proteins can activate transcription in a context-dependent manner, which does not establish activation by Dati. The raw donor ZNF208 is a different zinc-finger protein and cannot supply a target-specific regulatory direction.

Supporting Evidence:

PMID:25291190: "dati encodes a zinc finger transcription factor closely related to rotund (rn) and squeeze (sqz) with homologs in several species, including humans"
PMID:25291190: "Here we described DATI, a zinc finger transcription factor related to the Drosophila Rotund and Squeeze and the vertebrate ZNF384"
PMID:11867614: "the native Nmp4 protein up-regulated transcription"

GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding GO_MF

CNN — Correct but not novel Review score: 2/2

Prediction method: ProtNLM2 · Version: UniProt API snapshot 2026-09-08 · file:DROME/dati/dati-predictions-source.json

Review rationale: Dati is a nuclear zinc-finger transcriptional regulator related to Rotund/Squeeze and vertebrate ZNF384, with a retained C2H2 finger array. Direct DNA-binding studies of the characterized Nmp4/ZNF384 family support transfer of the broad cis-regulatory DNA-binding mechanism; the neuronal specification phenotype supplies coherent target biological context. This is an evolutionary inference rather than a mapped Dati binding motif or target promoter. The same broad DNA-binding transcription-regulatory role was already established in the Dati literature before the prediction, so this is correct but not novel; activation versus repression remains unresolved.

Supporting Evidence:

PMID:25291190: "dati encodes a zinc finger transcription factor closely related to rotund (rn) and squeeze (sqz) with homologs in several species, including humans"
PMID:25291190: "Here we described DATI, a zinc finger transcription factor related to the Drosophila Rotund and Squeeze and the vertebrate ZNF384"
PMID:11867614: "Zinc fingers 2, 3, and 6 mediated association with the homopolymeric (dA.dT) COL1A1/MMP DNA consensus element."

📚 Additional Documentation

Protnlm Function Review

(dati-protnlm-function-review.md)

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Gene Description

Existing Annotations Review

Core Functions

Acts as a zinc-finger transcriptional regulator supporting neuronal subtype specification and projection development; DNA-binding mechanism is inferred from the characterized family.

References

External Prediction Reviews

ProtNLM2 External predictions

Deep Research

Falcon

📚 Additional Documentation

Notes

Protnlm Function Review

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