Glorund is an RNA-binding regulator of translation and RNA processing in Drosophila oogenesis. The Q8INJ6 isoform-C record is a short 179-residue RRM-containing product, whereas gene-level experiments establish repression of nanos translation, association with Hrp48 and Half-pint, and roles in oocyte RNA localization. Which of these activities and localizations are retained by this short isoform remains unresolved.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0003676 nucleic acid binding | IEA GO_REF:0000002 | MODIFY | Summary: RNA binding is more specific than nucleic-acid binding. Reason: The exact product retains an RRM at residues 49-129 and belongs to the experimentally characterized RNA-binding Glorund locus. RNA binding is a reasonable domain-level inference without transferring a specific mRNA target. Proposed replacements: RNA binding Supporting Evidence: file:DROME/glo/glo-uniprot.txt DR InterPro; IPR050666; ESRP. file:DROME/glo/glo-uniprot.txt DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf. file:DROME/glo/glo-uniprot.txt FT DOMAIN 49..129 FT /note="RRM" FT /evidence="ECO:0000259|PROSITE:PS50102" PMID:19013444 whereas Glo and Hrp48 are both nuclear and cytoplasmic PMID:19013444 Here we show that Glorund is part of a complex containing the hnRNP protein Hrp48 and the splicing factor Half-pint |
| GO:0003723 RNA binding | IEA GO_REF:0000120 | ACCEPT | Summary: Generic RNA-binding potential is supported by the retained RRM. Reason: The 179-residue product contains an intact annotated RRM at residues 49-129. This supports broad RNA binding but does not identify its target RNAs or establish full-length Glorund activity. Supporting Evidence: file:DROME/glo/glo-uniprot.txt DR InterPro; IPR050666; ESRP. file:DROME/glo/glo-uniprot.txt DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf. file:DROME/glo/glo-uniprot.txt FT DOMAIN 49..129 FT /note="RRM" FT /evidence="ECO:0000259|PROSITE:PS50102" PMID:19013444 whereas Glo and Hrp48 are both nuclear and cytoplasmic PMID:19013444 Here we show that Glorund is part of a complex containing the hnRNP protein Hrp48 and the splicing factor Half-pint |
| GO:0003729 mRNA binding | ISS PMID:10908586 The drosophila melanogaster genome: translation factors and ... | UNDECIDED | Summary: Specific mRNA binding remains unresolved for the short isoform. Reason: PMID:10908586 surveys RNA-binding proteins at gene/family level; the exact Q8INJ6 product has only a short RRM-containing architecture and no isoform-mapped mRNA binding assay is established. Supporting Evidence: file:DROME/glo/glo-uniprot.txt DR InterPro; IPR050666; ESRP. file:DROME/glo/glo-uniprot.txt DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf. file:DROME/glo/glo-uniprot.txt FT DOMAIN 49..129 FT /note="RRM" FT /evidence="ECO:0000259|PROSITE:PS50102" PMID:19013444 whereas Glo and Hrp48 are both nuclear and cytoplasmic PMID:19013444 Here we show that Glorund is part of a complex containing the hnRNP protein Hrp48 and the splicing factor Half-pint |
| GO:0005515 protein binding | IPI PMID:19013444 Glorund interactions in the regulation of gurken and oskar m... | UNDECIDED | Summary: The Hrp48 interaction is established for Glorund but unresolved for this short product. Reason: PMID:19013444 identifies a Glorund-Hrp48-Half-pint complex. The 179-residue isoform needs construct mapping before these gene-level interactions can be assigned to it. Supporting Evidence: file:DROME/glo/glo-uniprot.txt DR InterPro; IPR050666; ESRP. file:DROME/glo/glo-uniprot.txt DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf. file:DROME/glo/glo-uniprot.txt FT DOMAIN 49..129 FT /note="RRM" FT /evidence="ECO:0000259|PROSITE:PS50102" PMID:19013444 whereas Glo and Hrp48 are both nuclear and cytoplasmic PMID:19013444 Here we show that Glorund is part of a complex containing the hnRNP protein Hrp48 and the splicing factor Half-pint |
| GO:0005515 protein binding | IPI PMID:19013444 Glorund interactions in the regulation of gurken and oskar m... | UNDECIDED | Summary: The Half-pint interaction is not mapped to the short isoform. Reason: The full-text complex experiments in PMID:19013444 establish Glorund-associated interactions, but do not establish retention of the interaction surfaces in the exact 179-residue product. Supporting Evidence: file:DROME/glo/glo-uniprot.txt DR InterPro; IPR050666; ESRP. file:DROME/glo/glo-uniprot.txt DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf. file:DROME/glo/glo-uniprot.txt FT DOMAIN 49..129 FT /note="RRM" FT /evidence="ECO:0000259|PROSITE:PS50102" PMID:19013444 whereas Glo and Hrp48 are both nuclear and cytoplasmic PMID:19013444 Here we show that Glorund is part of a complex containing the hnRNP protein Hrp48 and the splicing factor Half-pint |
| GO:0006417 regulation of translation | IMP PMID:16516833 Glorund, a Drosophila hnRNP F/H homolog, is an ovarian repre... | UNDECIDED | Summary: Translational repression is established at the locus level but not for this isoform. Reason: PMID:16516833 demonstrates Glorund-dependent nanos translational repression. Q8INJ6 contains a short RRM-bearing sequence and requires construct or isoform mapping before that activity is transferred. Supporting Evidence: file:DROME/glo/glo-uniprot.txt DR InterPro; IPR050666; ESRP. file:DROME/glo/glo-uniprot.txt DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf. file:DROME/glo/glo-uniprot.txt FT DOMAIN 49..129 FT /note="RRM" FT /evidence="ECO:0000259|PROSITE:PS50102" PMID:19013444 whereas Glo and Hrp48 are both nuclear and cytoplasmic PMID:19013444 Here we show that Glorund is part of a complex containing the hnRNP protein Hrp48 and the splicing factor Half-pint |
| GO:0007311 maternal specification of dorsal/ventral axis, oocyte, germ-line encoded | IMP PMID:19013444 Glorund interactions in the regulation of gurken and oskar m... | UNDECIDED | Summary: The developmental phenotype is not isoform-resolved. Reason: PMID:19013444 establishes Glorund roles in oocyte patterning; the responsible experimental products are not mapped to Q8INJ6 in the inspected evidence. Supporting Evidence: file:DROME/glo/glo-uniprot.txt DR InterPro; IPR050666; ESRP. file:DROME/glo/glo-uniprot.txt DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf. file:DROME/glo/glo-uniprot.txt FT DOMAIN 49..129 FT /note="RRM" FT /evidence="ECO:0000259|PROSITE:PS50102" PMID:19013444 whereas Glo and Hrp48 are both nuclear and cytoplasmic PMID:19013444 Here we show that Glorund is part of a complex containing the hnRNP protein Hrp48 and the splicing factor Half-pint |
| GO:0032991 protein-containing complex | IPI PMID:19013444 Glorund interactions in the regulation of gurken and oskar m... | UNDECIDED | Summary: The relevant complex membership is unresolved for the short product. Reason: The Glorund-Hrp48-Half-pint complex in PMID:19013444 is real gene-level evidence; incorporation of the 179-residue isoform is unestablished. Supporting Evidence: file:DROME/glo/glo-uniprot.txt DR InterPro; IPR050666; ESRP. file:DROME/glo/glo-uniprot.txt DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf. file:DROME/glo/glo-uniprot.txt FT DOMAIN 49..129 FT /note="RRM" FT /evidence="ECO:0000259|PROSITE:PS50102" PMID:19013444 whereas Glo and Hrp48 are both nuclear and cytoplasmic PMID:19013444 Here we show that Glorund is part of a complex containing the hnRNP protein Hrp48 and the splicing factor Half-pint |
| GO:0032991 protein-containing complex | IPI PMID:19013444 Glorund interactions in the regulation of gurken and oskar m... | UNDECIDED | Summary: The relevant complex membership is unresolved for the short product. Reason: The Glorund-Hrp48-Half-pint complex in PMID:19013444 is real gene-level evidence; incorporation of the 179-residue isoform is unestablished. Supporting Evidence: file:DROME/glo/glo-uniprot.txt DR InterPro; IPR050666; ESRP. file:DROME/glo/glo-uniprot.txt DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf. file:DROME/glo/glo-uniprot.txt FT DOMAIN 49..129 FT /note="RRM" FT /evidence="ECO:0000259|PROSITE:PS50102" PMID:19013444 whereas Glo and Hrp48 are both nuclear and cytoplasmic PMID:19013444 Here we show that Glorund is part of a complex containing the hnRNP protein Hrp48 and the splicing factor Half-pint |
| GO:0042060 wound healing | HMP PMID:19884309 Genetic screen in Drosophila melanogaster uncovers a novel s... | UNDECIDED | Summary: The wound-repair phenotype cannot be assigned specifically to this short isoform. Reason: PMID:19884309 is an epithelial-repair genetic screen and the cache is abstract-only. Neither the precise experiment nor isoform-specific contribution can be verified from that source. Supporting Evidence: file:DROME/glo/glo-uniprot.txt DR InterPro; IPR050666; ESRP. file:DROME/glo/glo-uniprot.txt DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf. file:DROME/glo/glo-uniprot.txt FT DOMAIN 49..129 FT /note="RRM" FT /evidence="ECO:0000259|PROSITE:PS50102" PMID:19013444 whereas Glo and Hrp48 are both nuclear and cytoplasmic PMID:19013444 Here we show that Glorund is part of a complex containing the hnRNP protein Hrp48 and the splicing factor Half-pint |
| GO:0045451 germ plasm oskar mRNA localization | IMP PMID:19013444 Glorund interactions in the regulation of gurken and oskar m... | UNDECIDED | Summary: Oskar-mRNA localization is a supported gene-level role but not isoform-resolved. Reason: PMID:19013444 establishes a Glorund-dependent mRNA-localization phenotype; an intact RRM alone does not show that the 179-residue isoform executes this process. Supporting Evidence: file:DROME/glo/glo-uniprot.txt DR InterPro; IPR050666; ESRP. file:DROME/glo/glo-uniprot.txt DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf. file:DROME/glo/glo-uniprot.txt FT DOMAIN 49..129 FT /note="RRM" FT /evidence="ECO:0000259|PROSITE:PS50102" PMID:19013444 whereas Glo and Hrp48 are both nuclear and cytoplasmic PMID:19013444 Here we show that Glorund is part of a complex containing the hnRNP protein Hrp48 and the splicing factor Half-pint |
| GO:0051276 chromosome organization | IMP PMID:19013444 Glorund interactions in the regulation of gurken and oskar m... | UNDECIDED | Summary: Nurse-cell chromosome organization is not established for this isoform. Reason: PMID:19013444 connects Glorund complexes and ovarian-tumor splicing to chromosome organization. The phenotype should not be transferred to the short isoform without construct mapping. Supporting Evidence: file:DROME/glo/glo-uniprot.txt DR InterPro; IPR050666; ESRP. file:DROME/glo/glo-uniprot.txt DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf. file:DROME/glo/glo-uniprot.txt FT DOMAIN 49..129 FT /note="RRM" FT /evidence="ECO:0000259|PROSITE:PS50102" PMID:19013444 whereas Glo and Hrp48 are both nuclear and cytoplasmic PMID:19013444 Here we show that Glorund is part of a complex containing the hnRNP protein Hrp48 and the splicing factor Half-pint |
| GO:0060810 intracellular mRNA localization involved in pattern specification process | IMP PMID:19013444 Glorund interactions in the regulation of gurken and oskar m... | UNDECIDED | Summary: Patterning-associated mRNA localization is not resolved for the exact isoform. Reason: The locus-level data in PMID:19013444 support Glorund biology, while the sequence represented by Q8INJ6 requires specific mapping to the assayed protein. Supporting Evidence: file:DROME/glo/glo-uniprot.txt DR InterPro; IPR050666; ESRP. file:DROME/glo/glo-uniprot.txt DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf. file:DROME/glo/glo-uniprot.txt FT DOMAIN 49..129 FT /note="RRM" FT /evidence="ECO:0000259|PROSITE:PS50102" PMID:19013444 whereas Glo and Hrp48 are both nuclear and cytoplasmic PMID:19013444 Here we show that Glorund is part of a complex containing the hnRNP protein Hrp48 and the splicing factor Half-pint |
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