Jumu is a nuclear forkhead transcription factor and chromatin-associated regulator. It recognizes Forkhead sites in cis-regulatory DNA and controls context-dependent gene expression, including repression of the Nidogen enhancer in the embryonic heart. Its dosage affects chromatin and nucleolar organization, while its transcriptional programs regulate asymmetric cell fates, cardiac progenitor divisions and wound-responsive JAK/STAT signaling.
IDA PMID:17412918 Genes required for mitotic spindle assembly in Drosophila S2...
ACCEPT
Summary: Jumu/DOM associates with chromosomal sites, including the chromocenter and specific euchromatic loci.
Reason: Jumu/DOM associates with chromosomal sites, including the chromocenter and specific euchromatic loci. These direct observations support the chromatin/polytene-chromosome assignments, and the dosage-dependent chromatin phenotype is consistent with an active chromatin-associated regulatory role.
IDA PMID:20213139 The winged-helix transcription factor JUMU regulates develop...
ACCEPT
Summary: Jumu/DOM associates with chromosomal sites, including the chromocenter and specific euchromatic loci.
Reason: Jumu/DOM associates with chromosomal sites, including the chromocenter and specific euchromatic loci. These direct observations support the chromatin/polytene-chromosome assignments, and the dosage-dependent chromatin phenotype is consistent with an active chromatin-associated regulatory role.
DOM protein is accumulated in the chromocenter and, as expected from a transcription factor, is found at specific euchromatic loci.
GO:0000976 transcription cis-regulatory region binding
IDA PMID:22378636 Differential regulation of mesodermal gene expression by Dro...
MODIFY
Summary: Jumu recognizes sequence-specific Forkhead sites in a functional cis-regulatory enhancer.
Reason: Jumu recognizes sequence-specific Forkhead sites in a functional cis-regulatory enhancer. Cis-regulatory region sequence-specific DNA binding is more precise than the generic DNA-binding or regulatory-region-binding term.
different combinations of Fkh sites, corresponding to two different sequence specificities, mediate the particular functions of each TF.
GO:0000987 cis-regulatory region sequence-specific DNA binding
IBA GO_REF:0000033
ACCEPT
Summary: Jumu is a forkhead transcription factor with directly characterized sequence-dependent enhancer regulation.
Reason: Jumu is a forkhead transcription factor with directly characterized sequence-dependent enhancer regulation. The primary Nidogen study shows cooperation with CHES-1-like to repress the cardiac enhancer through distinct Forkhead binding sites. This establishes regulatory DNA recognition and transcriptional control, rather than merely generic DNA association.
Summary: Jumu recognizes sequence-specific Forkhead sites in a functional cis-regulatory enhancer.
Reason: Jumu recognizes sequence-specific Forkhead sites in a functional cis-regulatory enhancer. Cis-regulatory region sequence-specific DNA binding is more precise than the generic DNA-binding or regulatory-region-binding term.
Summary: Jumu is a forkhead transcription factor with directly characterized sequence-dependent enhancer regulation.
Reason: Jumu is a forkhead transcription factor with directly characterized sequence-dependent enhancer regulation. The primary Nidogen study shows cooperation with CHES-1-like to repress the cardiac enhancer through distinct Forkhead binding sites. This establishes regulatory DNA recognition and transcriptional control, rather than merely generic DNA association.
Summary: Jumu is a forkhead transcription factor with directly characterized sequence-dependent enhancer regulation.
Reason: Jumu is a forkhead transcription factor with directly characterized sequence-dependent enhancer regulation. The primary Nidogen study shows cooperation with CHES-1-like to repress the cardiac enhancer through distinct Forkhead binding sites. This establishes regulatory DNA recognition and transcriptional control, rather than merely generic DNA association.
Summary: Direct microscopy places Jumu in nuclei, consistent with enhancer binding and transcriptional regulation.
Reason: Direct microscopy places Jumu in nuclei, consistent with enhancer binding and transcriptional regulation. This evidence independently supports the nuclear location also inferred phylogenetically.
IDA PMID:10887088 Jumeaux, a novel Drosophila winged-helix family protein, is ...
ACCEPT
Summary: Direct microscopy places Jumu in nuclei, consistent with enhancer binding and transcriptional regulation.
Reason: Direct microscopy places Jumu in nuclei, consistent with enhancer binding and transcriptional regulation. This evidence independently supports the nuclear location also inferred phylogenetically.
IDA PMID:27514026 The composition and organization of Drosophila heterochromat...
ACCEPT
Summary: Direct microscopy places Jumu in nuclei, consistent with enhancer binding and transcriptional regulation.
Reason: Direct microscopy places Jumu in nuclei, consistent with enhancer binding and transcriptional regulation. This evidence independently supports the nuclear location also inferred phylogenetically.
Summary: Direct microscopy places Jumu in nuclei, consistent with enhancer binding and transcriptional regulation.
Reason: Direct microscopy places Jumu in nuclei, consistent with enhancer binding and transcriptional regulation. This evidence independently supports the nuclear location also inferred phylogenetically.
IDA PMID:10940625 Domina (Dom), a new Drosophila member of the FKH/WH gene fam...
ACCEPT
Summary: Jumu/DOM associates with chromosomal sites, including the chromocenter and specific euchromatic loci.
Reason: Jumu/DOM associates with chromosomal sites, including the chromocenter and specific euchromatic loci. These direct observations support the chromatin/polytene-chromosome assignments, and the dosage-dependent chromatin phenotype is consistent with an active chromatin-associated regulatory role.
IDA PMID:10940625 Domina (Dom), a new Drosophila member of the FKH/WH gene fam...
ACCEPT
Summary: Jumu/DOM associates with chromosomal sites, including the chromocenter and specific euchromatic loci.
Reason: Jumu/DOM associates with chromosomal sites, including the chromocenter and specific euchromatic loci. These direct observations support the chromatin/polytene-chromosome assignments, and the dosage-dependent chromatin phenotype is consistent with an active chromatin-associated regulatory role.
IDA PMID:20213139 The winged-helix transcription factor JUMU regulates develop...
ACCEPT
Summary: Jumu/DOM associates with chromosomal sites, including the chromocenter and specific euchromatic loci.
Reason: Jumu/DOM associates with chromosomal sites, including the chromocenter and specific euchromatic loci. These direct observations support the chromatin/polytene-chromosome assignments, and the dosage-dependent chromatin phenotype is consistent with an active chromatin-associated regulatory role.
IDA PMID:10940625 Domina (Dom), a new Drosophila member of the FKH/WH gene fam...
KEEP AS NON CORE
Summary: Jumu has an observed nucleolar pool and its dosage affects nucleolar integrity.
Reason: Jumu has an observed nucleolar pool and its dosage affects nucleolar integrity. This additional nuclear localization is supported, but the best defined molecular function is sequence-specific transcriptional regulation.
IDA PMID:20213139 The winged-helix transcription factor JUMU regulates develop...
KEEP AS NON CORE
Summary: Jumu has an observed nucleolar pool and its dosage affects nucleolar integrity.
Reason: Jumu has an observed nucleolar pool and its dosage affects nucleolar integrity. This additional nuclear localization is supported, but the best defined molecular function is sequence-specific transcriptional regulation.
IMP PMID:20213139 The winged-helix transcription factor JUMU regulates develop...
ACCEPT
Summary: Jumu dosage affects chromatin organization and position-effect variegation.
Reason: Jumu dosage affects chromatin organization and position-effect variegation. Overexpression changes euchromatic chromosome arms and the chromocenter, connecting this chromatin-associated transcription factor to chromosome organization.
Overexpression of jumu results in bloating of euchromatic chromosome arms, displacement of the JUMU protein from the chromocenter and the nucleolus, fragile weak points, and disrupted chromocenter of polytene chromosomes.
GO:0006355 regulation of DNA-templated transcription
IBA GO_REF:0000033
ACCEPT
Summary: Jumu is a forkhead transcription factor with directly characterized sequence-dependent enhancer regulation.
Reason: Jumu is a forkhead transcription factor with directly characterized sequence-dependent enhancer regulation. The primary Nidogen study shows cooperation with CHES-1-like to repress the cardiac enhancer through distinct Forkhead binding sites. This establishes regulatory DNA recognition and transcriptional control, rather than merely generic DNA association.
different combinations of Fkh sites, corresponding to two different sequence specificities, mediate the particular functions of each TF.
GO:0006355 regulation of DNA-templated transcription
IEA GO_REF:0000002
ACCEPT
Summary: Jumu is a forkhead transcription factor with directly characterized sequence-dependent enhancer regulation.
Reason: Jumu is a forkhead transcription factor with directly characterized sequence-dependent enhancer regulation. The primary Nidogen study shows cooperation with CHES-1-like to repress the cardiac enhancer through distinct Forkhead binding sites. This establishes regulatory DNA recognition and transcriptional control, rather than merely generic DNA association.
IMP PMID:20213139 The winged-helix transcription factor JUMU regulates develop...
KEEP AS NON CORE
Summary: Correct Jumu dosage is required for nucleolar integrity, and overexpression of its acidic C terminus disrupts the nucleolus.
Reason: Correct Jumu dosage is required for nucleolar integrity, and overexpression of its acidic C terminus disrupts the nucleolus. This supported phenotype does not establish a distinct ribosome-processing catalytic function.
TAS PMID:12147138 Signaling pathways directing the movement and fusion of epit...
UNDECIDED
Summary: The dorsal-closure annotation cites a review whose cached abstract describes the general epithelial-fusion signaling network but does not resolve the Jumu-specific evidence.
Reason: The dorsal-closure annotation cites a review whose cached abstract describes the general epithelial-fusion signaling network but does not resolve the Jumu-specific evidence. A target-specific primary result or the relevant full-text passage is needed to assess this particular developmental role.
IMP PMID:10940625 Domina (Dom), a new Drosophila member of the FKH/WH gene fam...
KEEP AS NON CORE
Summary: Jumu/DOM mutants have rough eyes and wing-shape/margin defects, supporting these developmental roles.
Reason: Jumu/DOM mutants have rough eyes and wing-shape/margin defects, supporting these developmental roles. They are downstream tissue outcomes of a pleiotropic transcription and chromatin regulator rather than separate molecular activities.
IMP PMID:34999256 Jumu is required for the activation of JAK/STAT in Drosophil...
KEEP AS NON CORE
Summary: Jumu is required for wound-induced JAK/STAT activation and lymph-gland signaling.
Reason: Jumu is required for wound-induced JAK/STAT activation and lymph-gland signaling. This establishes a regulatory role upstream of the pathway under tissue-damage conditions, without implying that Jumu itself is a kinase or wound sensor.
IMP PMID:22814603 Two forkhead transcription factors regulate the division of ...
KEEP AS NON CORE
Summary: Jumu and CHES-1-like regulate symmetric and asymmetric cardiac-progenitor divisions through Polo activity.
Reason: Jumu and CHES-1-like regulate symmetric and asymmetric cardiac-progenitor divisions through Polo activity. The genetic data support heart development and division control as specific biological consequences of their transcriptional program.
Summary: Jumu recognizes sequence-specific Forkhead sites in a functional cis-regulatory enhancer.
Reason: Jumu recognizes sequence-specific Forkhead sites in a functional cis-regulatory enhancer. Cis-regulatory region sequence-specific DNA binding is more precise than the generic DNA-binding or regulatory-region-binding term.
different combinations of Fkh sites, corresponding to two different sequence specificities, mediate the particular functions of each TF.
GO:0046427 positive regulation of receptor signaling pathway via JAK-STAT
IMP PMID:34999256 Jumu is required for the activation of JAK/STAT in Drosophil...
KEEP AS NON CORE
Summary: Jumu is required for wound-induced JAK/STAT activation and lymph-gland signaling.
Reason: Jumu is required for wound-induced JAK/STAT activation and lymph-gland signaling. This establishes a regulatory role upstream of the pathway under tissue-damage conditions, without implying that Jumu itself is a kinase or wound sensor.
IMP PMID:10940625 Domina (Dom), a new Drosophila member of the FKH/WH gene fam...
KEEP AS NON CORE
Summary: Jumu/DOM mutants have rough eyes and wing-shape/margin defects, supporting these developmental roles.
Reason: Jumu/DOM mutants have rough eyes and wing-shape/margin defects, supporting these developmental roles. They are downstream tissue outcomes of a pleiotropic transcription and chromatin regulator rather than separate molecular activities.
IMP PMID:22814603 Two forkhead transcription factors regulate the division of ...
KEEP AS NON CORE
Summary: Jumu and CHES-1-like regulate symmetric and asymmetric cardiac-progenitor divisions through Polo activity.
Reason: Jumu and CHES-1-like regulate symmetric and asymmetric cardiac-progenitor divisions through Polo activity. The genetic data support heart development and division control as specific biological consequences of their transcriptional program.
These computational predictions are reviewed separately from the GOA annotation set used for this review. The assessments below are from this project and do not constitute official GO annotations or endorsement by GO/UniProt. They are not included in the existing annotation review above.
LSP — Less precise than existing annotation Review score: 2/2
Prediction method: ProtNLM2 · Version: UniProt API snapshot 2026-09-08
Review rationale: Sequence-specific DNA binding is directly supported by the Jumu/Forkhead-site analysis of the Nidogen enhancer. Jumu cooperates with CHES-1-like in cardiac repression, with distinct binding-site combinations determining regulatory activity. The existing cis-regulatory region sequence-specific DNA-binding annotation is more informative than the broad prediction.
Supporting Evidence:
PMID:22378636: "CHES-1-like cooperates with Jumu to repress this enhancer in the heart."
PMID:22378636: "different combinations of Fkh sites, corresponding to two different sequence specificities, mediate the particular functions of each TF."