Orb is a cytoplasmic polyadenylation element-binding protein that recognizes sequences in mRNA 3-prime untranslated regions and controls maternal mRNA translation and localization. It is enriched in the developing oocyte, where its post-transcriptional regulation supports germline development and establishment of oocyte polarity. Its C-terminal RNA-recognition region is conserved in the shorter Q8IMZ2 form.
Summary: Orb recognizes cytoplasmic polyadenylation elements in mRNA 3-prime untranslated regions. This experimentally defined RNA target makes mRNA 3-prime-UTR binding more informative than the generic nucleic-acid or RNA-binding annotation.
Reason: Orb recognizes cytoplasmic polyadenylation elements in mRNA 3-prime untranslated regions. This experimentally defined RNA target makes mRNA 3-prime-UTR binding more informative than the generic nucleic-acid or RNA-binding annotation.
Both Orb and Orb2 bind linear cytoplasmic polyadenylation element-like sequences in the 3' UTRs of largely overlapping target mRNAs, with Orb2 potentially having a broader specificity.
Summary: Orb recognizes cytoplasmic polyadenylation elements in mRNA 3-prime untranslated regions. This experimentally defined RNA target makes mRNA 3-prime-UTR binding more informative than the generic nucleic-acid or RNA-binding annotation.
Reason: Orb recognizes cytoplasmic polyadenylation elements in mRNA 3-prime untranslated regions. This experimentally defined RNA target makes mRNA 3-prime-UTR binding more informative than the generic nucleic-acid or RNA-binding annotation.
Both Orb and Orb2 bind linear cytoplasmic polyadenylation element-like sequences in the 3' UTRs of largely overlapping target mRNAs, with Orb2 potentially having a broader specificity.
TAS PMID:11700288 Translational regulation and RNA localization in Drosophila ...
MODIFY
Summary: Orb recognizes cytoplasmic polyadenylation elements in mRNA 3-prime untranslated regions. This experimentally defined RNA target makes mRNA 3-prime-UTR binding more informative than the generic nucleic-acid or RNA-binding annotation.
Reason: Orb recognizes cytoplasmic polyadenylation elements in mRNA 3-prime untranslated regions. This experimentally defined RNA target makes mRNA 3-prime-UTR binding more informative than the generic nucleic-acid or RNA-binding annotation.
Both Orb and Orb2 bind linear cytoplasmic polyadenylation element-like sequences in the 3' UTRs of largely overlapping target mRNAs, with Orb2 potentially having a broader specificity.
Summary: Direct binding profiles establish recognition of CPE-like sequences in mRNA 3-prime UTRs. The selected shorter Orb sequence retains the complete C-terminal RNA-binding region.
Reason: Direct binding profiles establish recognition of CPE-like sequences in mRNA 3-prime UTRs. The selected shorter Orb sequence retains the complete C-terminal RNA-binding region.
Both Orb and Orb2 bind linear cytoplasmic polyadenylation element-like sequences in the 3' UTRs of largely overlapping target mRNAs, with Orb2 potentially having a broader specificity.
IDA PMID:10101171 The posterior determinant gene nanos is required for the mai...
ACCEPT
Summary: Primary imaging localizes Orb to the cytoplasm of the germline cyst and enriches it in the oocyte. This directly supports the broad cytoplasmic localization.
Reason: Primary imaging localizes Orb to the cytoplasm of the germline cyst and enriches it in the oocyte. This directly supports the broad cytoplasmic localization.
Summary: Primary imaging localizes Orb to the cytoplasm of the germline cyst and enriches it in the oocyte. This directly supports the broad cytoplasmic localization.
Reason: Primary imaging localizes Orb to the cytoplasm of the germline cyst and enriches it in the oocyte. This directly supports the broad cytoplasmic localization.
Summary: Orb binds target mRNAs and controls their translation in the female germline. The conserved RNA-binding machinery in the selected form supports the broad regulator activity, without assuming identical regulation of all isoforms.
Reason: Orb binds target mRNAs and controls their translation in the female germline. The conserved RNA-binding machinery in the selected form supports the broad regulator activity, without assuming identical regulation of all isoforms.
Both Orb and Orb2 bind linear cytoplasmic polyadenylation element-like sequences in the 3' UTRs of largely overlapping target mRNAs, with Orb2 potentially having a broader specificity.
IMP PMID:11809824 Cytoplasmic localization and evolutionary conservation of ME...
KEEP AS NON CORE
Summary: The primary MEI-218 study directly examines orb-mutant germline cysts and supports an Orb requirement in oocyte development. This developmental consequence is retained as noncore relative to sequence-specific translational regulation.
Reason: The primary MEI-218 study directly examines orb-mutant germline cysts and supports an Orb requirement in oocyte development. This developmental consequence is retained as noncore relative to sequence-specific translational regulation.
IMP PMID:15737929 The Drosophila fragile X protein functions as a negative reg...
KEEP AS NON CORE
Summary: Orb-dependent post-transcriptional regulation supports oocyte polarity and localization of maternal determinants, including oskar RNA. Retain the experimentally supported developmental/localization consequence as noncore relative to its direct RNA-binding and translational-regulatory mechanism.
Reason: Orb-dependent post-transcriptional regulation supports oocyte polarity and localization of maternal determinants, including oskar RNA. Retain the experimentally supported developmental/localization consequence as noncore relative to its direct RNA-binding and translational-regulatory mechanism.
IMP PMID:7926753 orb is required for anteroposterior and dorsoventral pattern...
KEEP AS NON CORE
Summary: Orb-dependent post-transcriptional regulation supports oocyte polarity and localization of maternal determinants, including oskar RNA. Retain the experimentally supported developmental/localization consequence as noncore relative to its direct RNA-binding and translational-regulatory mechanism.
Reason: Orb-dependent post-transcriptional regulation supports oocyte polarity and localization of maternal determinants, including oskar RNA. Retain the experimentally supported developmental/localization consequence as noncore relative to its direct RNA-binding and translational-regulatory mechanism.
IMP PMID:7926753 orb is required for anteroposterior and dorsoventral pattern...
KEEP AS NON CORE
Summary: Orb-dependent post-transcriptional regulation supports oocyte polarity and localization of maternal determinants, including oskar RNA. Retain the experimentally supported developmental/localization consequence as noncore relative to its direct RNA-binding and translational-regulatory mechanism.
Reason: Orb-dependent post-transcriptional regulation supports oocyte polarity and localization of maternal determinants, including oskar RNA. Retain the experimentally supported developmental/localization consequence as noncore relative to its direct RNA-binding and translational-regulatory mechanism.
Summary: Orb binds target mRNAs and controls their translation in the female germline. The conserved RNA-binding machinery in the selected form supports the broad regulator activity, without assuming identical regulation of all isoforms.
Reason: Orb binds target mRNAs and controls their translation in the female germline. The conserved RNA-binding machinery in the selected form supports the broad regulator activity, without assuming identical regulation of all isoforms.
Both Orb and Orb2 bind linear cytoplasmic polyadenylation element-like sequences in the 3' UTRs of largely overlapping target mRNAs, with Orb2 potentially having a broader specificity.
IMP PMID:7926753 orb is required for anteroposterior and dorsoventral pattern...
KEEP AS NON CORE
Summary: Orb-dependent post-transcriptional regulation supports oocyte polarity and localization of maternal determinants, including oskar RNA. Retain the experimentally supported developmental/localization consequence as noncore relative to its direct RNA-binding and translational-regulatory mechanism.
Reason: Orb-dependent post-transcriptional regulation supports oocyte polarity and localization of maternal determinants, including oskar RNA. Retain the experimentally supported developmental/localization consequence as noncore relative to its direct RNA-binding and translational-regulatory mechanism.
Both Orb and Orb2 bind linear cytoplasmic polyadenylation element-like sequences in the 3' UTRs of largely overlapping target mRNAs, with Orb2 potentially having a broader specificity.
These computational predictions are reviewed separately from the GOA annotation set used for this review. The assessments below are from this project and do not constitute official GO annotations or endorsement by GO/UniProt. They are not included in the existing annotation review above.
Review rationale: Orb is an experimentally established CPEB-family RNA-binding translation regulator, and the same GO term already occurs in the selected record. Direct RNA-target profiling and germline translational studies support correctness independently of that electronic annotation. Q8IMZ2 is shorter at the N-terminus but retains the complete C-terminal RNA-binding machinery, making transfer of this broad function reasonable. The prediction adds no novel activity.
Supporting Evidence:
PMID:27791065: "Both Orb and Orb2 bind linear cytoplasmic polyadenylation element-like sequences in the 3' UTRs of largely overlapping target mRNAs, with Orb2 potentially having a broader specificity."
PMID:15737929: "Orb is responsible for mediating the translational activation of mRNAs localized within the developing oocyte."