Piwi is the nuclear PIWI-clade Argonaute of Drosophila, expressed in ovarian somatic follicle and germline cells and in testes. Loaded in the cytoplasm (Yb bodies and on mitochondria, with Armitage and Zucchini-dependent processing) with antisense, 5-uridine primary/phased piRNAs, mature Piwi-piRISC is imported into the nucleus, where it scans nascent transcripts and, via Asterix/Gtsf1, Panoramix/SFiNX, SUMOylation and Eggless/SetDB1, establishes H3K9me3 heterochromatin and co-transcriptional silencing of transposons. Its slicer activity exists in vitro but is dispensable for silencing. Piwi is also required in somatic niche cells and germline for germline stem cell self-renewal, and maternal Piwi in polar granules contributes to pole cell formation. In a complex with Hsp83 (Hsp90) and Hop, Piwi also contributes to canalization, buffering phenotypic variation, which is attributed to epigenetic silencing of existing genetic variants and suppression of transposon-induced new variation.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0000785 chromatin | IDA PMID:23392610 Piwi induces piRNA-guided transcriptional silencing and esta... | ACCEPT | Summary: Piwi associates with chromatin at target loci. Reason: Nuclear piRNA-loaded Piwi is recruited to nascent transcripts at transposon loci and associates with chromatin. Supporting Evidence: PMID:23392610 Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. |
| GO:0000791 euchromatin | IDA PMID:17875665 Drosophila PIWI associates with chromatin and interacts dire... | KEEP AS NON CORE | Summary: Piwi on polytene euchromatin (PMID:17875665). Reason: Piwi associates with euchromatic sites as well as heterochromatin; a localization detail. Supporting Evidence: PMID:17875665 These findings implicate a direct interaction between the PIWI-mediated small RNA mechanism and heterochromatin-forming pathways in determining the epigenetic state of the fly genome. |
| GO:0000792 heterochromatin | IDA PMID:17875665 Drosophila PIWI associates with chromatin and interacts dire... | ACCEPT | Summary: Piwi associates with heterochromatin. Reason: Piwi interacts with HP1a and is associated with heterochromatic regions it helps establish. Supporting Evidence: PMID:17875665 These findings implicate a direct interaction between the PIWI-mediated small RNA mechanism and heterochromatin-forming pathways in determining the epigenetic state of the fly genome. |
| GO:0003676 nucleic acid binding | IEA GO_REF:0000002 | MODIFY | Summary: InterPro nucleic acid binding. Reason: Correct but too general. Piwi is a PIWI-clade Argonaute whose defining nucleic-acid-binding activity is loading of piRNA guides; piRNA binding is the informative replacement. Proposed replacements: piRNA binding Supporting Evidence: PMID:16882972 These data together imply that Piwi functions in nuclear RNA silencing as Slicer by associating specifically with rasiRNAs originating from repetitive targets. PMID:17346786 Piwi and Aubergine bind RNAs that are predominantly antisense to transposons |
| GO:0003723 RNA binding | IDA PMID:28472469 Piwi interacts with chromatin at nuclear pores and promiscuo... | ACCEPT | Summary: Piwi binds nuclear transcripts (PMID:28472469). Reason: Piwi-piRNA complexes bind nascent target RNAs; RNA binding beyond the guide is part of target recognition. Supporting Evidence: PMID:28472469 Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells. |
| GO:0003723 RNA binding | IEA GO_REF:0000002 | ACCEPT | Summary: InterPro RNA binding. Reason: Correct: Piwi binds piRNA guides and nascent target RNAs; piRNA binding is the more specific MF. Supporting Evidence: PMID:16882972 These data together imply that Piwi functions in nuclear RNA silencing as Slicer by associating specifically with rasiRNAs originating from repetitive targets. PMID:17346786 Piwi and Aubergine bind RNAs that are predominantly antisense to transposons |
| GO:0004521 RNA endonuclease activity | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: Piwi has in vitro slicer activity. Reason: Recombinant Piwi cleaves target RNA in vitro (PMID:16882972), but Piwi-mediated transposon silencing requires its nuclear localization and not its slicer activity. The activity exists but is not the mechanism of the core function. Supporting Evidence: PMID:22902557 Mature Piwi-RISC triggers TE silencing by an unknown mechanism that requires Piwi’s nuclear localization but not its slicer activity PMID:16882972 In vitro, recombinant Piwi exhibits target RNA cleavage activity. |
| GO:0004521 RNA endonuclease activity | IDA PMID:16882972 Specific association of Piwi with rasiRNAs derived from retr... | KEEP AS NON CORE | Summary: Piwi has in vitro slicer activity. Reason: Recombinant Piwi cleaves target RNA in vitro (PMID:16882972), but Piwi-mediated transposon silencing requires its nuclear localization and not its slicer activity. The activity exists but is not the mechanism of the core function. Supporting Evidence: PMID:22902557 Mature Piwi-RISC triggers TE silencing by an unknown mechanism that requires Piwi’s nuclear localization but not its slicer activity PMID:16882972 In vitro, recombinant Piwi exhibits target RNA cleavage activity. |
| GO:0004521 RNA endonuclease activity | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Piwi has in vitro slicer activity. Reason: Recombinant Piwi cleaves target RNA in vitro (PMID:16882972), but Piwi-mediated transposon silencing requires its nuclear localization and not its slicer activity. The activity exists but is not the mechanism of the core function. Supporting Evidence: PMID:22902557 Mature Piwi-RISC triggers TE silencing by an unknown mechanism that requires Piwi’s nuclear localization but not its slicer activity PMID:16882972 In vitro, recombinant Piwi exhibits target RNA cleavage activity. |
| GO:0004521 RNA endonuclease activity | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: Piwi has in vitro slicer activity. Reason: Recombinant Piwi cleaves target RNA in vitro (PMID:16882972), but Piwi-mediated transposon silencing requires its nuclear localization and not its slicer activity. The activity exists but is not the mechanism of the core function. Supporting Evidence: PMID:22902557 Mature Piwi-RISC triggers TE silencing by an unknown mechanism that requires Piwi’s nuclear localization but not its slicer activity PMID:16882972 In vitro, recombinant Piwi exhibits target RNA cleavage activity. |
| GO:0005515 protein binding | IPI PMID:16949822 The role of PIWI and the miRNA machinery in Drosophila germl... | REMOVE | Summary: Protein-protein interaction of Piwi with a piRNA-pathway cofactor (PMID:16949822). Reason: Generic protein binding is uninformative about Piwi function. The reported partners (e.g. Papi, Vreteno, Panoramix/Asterix, Nxf2/SFiNX, Nup358, HP1a-pathway factors) are real piRNA-pathway cofactors, but the interaction alone does not establish a more specific Piwi molecular function. Removal does not imply the interaction is false. |
| GO:0005515 protein binding | IPI PMID:16949822 The role of PIWI and the miRNA machinery in Drosophila germl... | REMOVE | Summary: Protein-protein interaction of Piwi with a piRNA-pathway cofactor (PMID:16949822). Reason: Generic protein binding is uninformative about Piwi function. The reported partners (e.g. Papi, Vreteno, Panoramix/Asterix, Nxf2/SFiNX, Nup358, HP1a-pathway factors) are real piRNA-pathway cofactors, but the interaction alone does not establish a more specific Piwi molecular function. Removal does not imply the interaction is false. |
| GO:0005515 protein binding | IPI PMID:16949822 The role of PIWI and the miRNA machinery in Drosophila germl... | REMOVE | Summary: Protein-protein interaction of Piwi with a piRNA-pathway cofactor (PMID:16949822). Reason: Generic protein binding is uninformative about Piwi function. The reported partners (e.g. Papi, Vreteno, Panoramix/Asterix, Nxf2/SFiNX, Nup358, HP1a-pathway factors) are real piRNA-pathway cofactors, but the interaction alone does not establish a more specific Piwi molecular function. Removal does not imply the interaction is false. |
| GO:0005515 protein binding | IPI PMID:20818334 An in vivo RNAi assay identifies major genetic and cellular ... | REMOVE | Summary: Protein-protein interaction of Piwi with a piRNA-pathway cofactor (PMID:20818334). Reason: Generic protein binding is uninformative about Piwi function. The reported partners (e.g. Papi, Vreteno, Panoramix/Asterix, Nxf2/SFiNX, Nup358, HP1a-pathway factors) are real piRNA-pathway cofactors, but the interaction alone does not establish a more specific Piwi molecular function. Removal does not imply the interaction is false. |
| GO:0005515 protein binding | IPI PMID:21447556 PAPI, a novel TUDOR-domain protein, complexes with AGO3, ME3... | REMOVE | Summary: Protein-protein interaction of Piwi with a piRNA-pathway cofactor (PMID:21447556). Reason: Generic protein binding is uninformative about Piwi function. The reported partners (e.g. Papi, Vreteno, Panoramix/Asterix, Nxf2/SFiNX, Nup358, HP1a-pathway factors) are real piRNA-pathway cofactors, but the interaction alone does not establish a more specific Piwi molecular function. Removal does not imply the interaction is false. |
| GO:0005515 protein binding | IPI PMID:21863019 A systematic analysis of Drosophila TUDOR domain-containing ... | REMOVE | Summary: Protein-protein interaction of Piwi with a piRNA-pathway cofactor (PMID:21863019). Reason: Generic protein binding is uninformative about Piwi function. The reported partners (e.g. Papi, Vreteno, Panoramix/Asterix, Nxf2/SFiNX, Nup358, HP1a-pathway factors) are real piRNA-pathway cofactors, but the interaction alone does not establish a more specific Piwi molecular function. Removal does not imply the interaction is false. |
| GO:0005515 protein binding | IPI PMID:26472911 Panoramix enforces piRNA-dependent cotranscriptional silenci... | REMOVE | Summary: Protein-protein interaction of Piwi with a piRNA-pathway cofactor (PMID:26472911). Reason: Generic protein binding is uninformative about Piwi function. The reported partners (e.g. Papi, Vreteno, Panoramix/Asterix, Nxf2/SFiNX, Nup358, HP1a-pathway factors) are real piRNA-pathway cofactors, but the interaction alone does not establish a more specific Piwi molecular function. Removal does not imply the interaction is false. |
| GO:0005515 protein binding | IPI PMID:28472469 Piwi interacts with chromatin at nuclear pores and promiscuo... | REMOVE | Summary: Protein-protein interaction of Piwi with a piRNA-pathway cofactor (PMID:28472469). Reason: Generic protein binding is uninformative about Piwi function. The reported partners (e.g. Papi, Vreteno, Panoramix/Asterix, Nxf2/SFiNX, Nup358, HP1a-pathway factors) are real piRNA-pathway cofactors, but the interaction alone does not establish a more specific Piwi molecular function. Removal does not imply the interaction is false. |
| GO:0005515 protein binding | IPI PMID:28472469 Piwi interacts with chromatin at nuclear pores and promiscuo... | REMOVE | Summary: Protein-protein interaction of Piwi with a piRNA-pathway cofactor (PMID:28472469). Reason: Generic protein binding is uninformative about Piwi function. The reported partners (e.g. Papi, Vreteno, Panoramix/Asterix, Nxf2/SFiNX, Nup358, HP1a-pathway factors) are real piRNA-pathway cofactors, but the interaction alone does not establish a more specific Piwi molecular function. Removal does not imply the interaction is false. |
| GO:0005515 protein binding | IPI PMID:28472469 Piwi interacts with chromatin at nuclear pores and promiscuo... | REMOVE | Summary: Protein-protein interaction of Piwi with a piRNA-pathway cofactor (PMID:28472469). Reason: Generic protein binding is uninformative about Piwi function. The reported partners (e.g. Papi, Vreteno, Panoramix/Asterix, Nxf2/SFiNX, Nup358, HP1a-pathway factors) are real piRNA-pathway cofactors, but the interaction alone does not establish a more specific Piwi molecular function. Removal does not imply the interaction is false. |
| GO:0005515 protein binding | IPI PMID:29531043 Structural insights into the sequence-specific recognition o... | REMOVE | Summary: Protein-protein interaction of Piwi with a piRNA-pathway cofactor (PMID:29531043). Reason: Generic protein binding is uninformative about Piwi function. The reported partners (e.g. Papi, Vreteno, Panoramix/Asterix, Nxf2/SFiNX, Nup358, HP1a-pathway factors) are real piRNA-pathway cofactors, but the interaction alone does not establish a more specific Piwi molecular function. Removal does not imply the interaction is false. |
| GO:0005515 protein binding | IPI PMID:29735528 A critical role for nucleoporin 358 (Nup358) in transposon s... | REMOVE | Summary: Protein-protein interaction of Piwi with a piRNA-pathway cofactor (PMID:29735528). Reason: Generic protein binding is uninformative about Piwi function. The reported partners (e.g. Papi, Vreteno, Panoramix/Asterix, Nxf2/SFiNX, Nup358, HP1a-pathway factors) are real piRNA-pathway cofactors, but the interaction alone does not establish a more specific Piwi molecular function. Removal does not imply the interaction is false. |
| GO:0005515 protein binding | IPI PMID:31368590 Nuclear RNA export factor variant initiates piRNA-guided co-... | REMOVE | Summary: Protein-protein interaction of Piwi with a piRNA-pathway cofactor (PMID:31368590). Reason: Generic protein binding is uninformative about Piwi function. The reported partners (e.g. Papi, Vreteno, Panoramix/Asterix, Nxf2/SFiNX, Nup358, HP1a-pathway factors) are real piRNA-pathway cofactors, but the interaction alone does not establish a more specific Piwi molecular function. Removal does not imply the interaction is false. |
| GO:0005515 protein binding | IPI PMID:31384064 The nascent RNA binding complex SFiNX licenses piRNA-guided ... | REMOVE | Summary: Protein-protein interaction of Piwi with a piRNA-pathway cofactor (PMID:31384064). Reason: Generic protein binding is uninformative about Piwi function. The reported partners (e.g. Papi, Vreteno, Panoramix/Asterix, Nxf2/SFiNX, Nup358, HP1a-pathway factors) are real piRNA-pathway cofactors, but the interaction alone does not establish a more specific Piwi molecular function. Removal does not imply the interaction is false. |
| GO:0005634 nucleus | IBA GO_REF:0000033 | ACCEPT | Summary: Piwi is a nuclear PIWI protein. Reason: Core location: piRNA-loaded Piwi is imported into the nucleus, where it directs co-transcriptional silencing. Supporting Evidence: PMID:22902557 Formation of mature Piwi-RISC triggers its nuclear import, while failure in piRNA biogenesis results in destabilization of presumably unloaded Piwi PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0005634 nucleus | IDA PMID:16530043 RNAi components are required for nuclear clustering of Polyc... | ACCEPT | Summary: Piwi is a nuclear PIWI protein. Reason: Core location: piRNA-loaded Piwi is imported into the nucleus, where it directs co-transcriptional silencing. Supporting Evidence: PMID:22902557 Formation of mature Piwi-RISC triggers its nuclear import, while failure in piRNA biogenesis results in destabilization of presumably unloaded Piwi PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0005634 nucleus | IDA PMID:16882972 Specific association of Piwi with rasiRNAs derived from retr... | ACCEPT | Summary: Piwi is a nuclear PIWI protein. Reason: Core location: piRNA-loaded Piwi is imported into the nucleus, where it directs co-transcriptional silencing. Supporting Evidence: PMID:22902557 Formation of mature Piwi-RISC triggers its nuclear import, while failure in piRNA biogenesis results in destabilization of presumably unloaded Piwi PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0005634 nucleus | IDA PMID:17346786 Discrete small RNA-generating loci as master regulators of t... | ACCEPT | Summary: Piwi is a nuclear PIWI protein. Reason: Core location: piRNA-loaded Piwi is imported into the nucleus, where it directs co-transcriptional silencing. Supporting Evidence: PMID:22902557 Formation of mature Piwi-RISC triggers its nuclear import, while failure in piRNA biogenesis results in destabilization of presumably unloaded Piwi PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0005634 nucleus | IDA PMID:21106531 The Yb body, a major site for Piwi-associated RNA biogenesis... | ACCEPT | Summary: Piwi is a nuclear PIWI protein. Reason: Core location: piRNA-loaded Piwi is imported into the nucleus, where it directs co-transcriptional silencing. Supporting Evidence: PMID:22902557 Formation of mature Piwi-RISC triggers its nuclear import, while failure in piRNA biogenesis results in destabilization of presumably unloaded Piwi PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0005634 nucleus | IDA PMID:21831924 Vreteno, a gonad-specific protein, is essential for germline... | ACCEPT | Summary: Piwi is a nuclear PIWI protein. Reason: Core location: piRNA-loaded Piwi is imported into the nucleus, where it directs co-transcriptional silencing. Supporting Evidence: PMID:22902557 Formation of mature Piwi-RISC triggers its nuclear import, while failure in piRNA biogenesis results in destabilization of presumably unloaded Piwi PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0005634 nucleus | IDA PMID:22065765 Separation of stem cell maintenance and transposon silencing... | ACCEPT | Summary: Piwi is a nuclear PIWI protein. Reason: Core location: piRNA-loaded Piwi is imported into the nucleus, where it directs co-transcriptional silencing. Supporting Evidence: PMID:22902557 Formation of mature Piwi-RISC triggers its nuclear import, while failure in piRNA biogenesis results in destabilization of presumably unloaded Piwi PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0005634 nucleus | IDA PMID:22902557 The cochaperone shutdown defines a group of biogenesis facto... | ACCEPT | Summary: Piwi is a nuclear PIWI protein. Reason: Core location: piRNA-loaded Piwi is imported into the nucleus, where it directs co-transcriptional silencing. Supporting Evidence: PMID:22902557 Formation of mature Piwi-RISC triggers its nuclear import, while failure in piRNA biogenesis results in destabilization of presumably unloaded Piwi PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0005634 nucleus | IDA PMID:23392610 Piwi induces piRNA-guided transcriptional silencing and esta... | ACCEPT | Summary: Piwi is a nuclear PIWI protein. Reason: Core location: piRNA-loaded Piwi is imported into the nucleus, where it directs co-transcriptional silencing. Supporting Evidence: PMID:22902557 Formation of mature Piwi-RISC triggers its nuclear import, while failure in piRNA biogenesis results in destabilization of presumably unloaded Piwi PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0005634 nucleus | IDA PMID:26494711 Silencio/CG9754 connects the Piwi-piRNA complex to the cellu... | ACCEPT | Summary: Piwi is a nuclear PIWI protein. Reason: Core location: piRNA-loaded Piwi is imported into the nucleus, where it directs co-transcriptional silencing. Supporting Evidence: PMID:22902557 Formation of mature Piwi-RISC triggers its nuclear import, while failure in piRNA biogenesis results in destabilization of presumably unloaded Piwi PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0005634 nucleus | IDA PMID:26808625 Tudor-SN Interacts with Piwi Antagonistically in Regulating ... | ACCEPT | Summary: Piwi is a nuclear PIWI protein. Reason: Core location: piRNA-loaded Piwi is imported into the nucleus, where it directs co-transcriptional silencing. Supporting Evidence: PMID:22902557 Formation of mature Piwi-RISC triggers its nuclear import, while failure in piRNA biogenesis results in destabilization of presumably unloaded Piwi PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0005634 nucleus | IDA PMID:28472469 Piwi interacts with chromatin at nuclear pores and promiscuo... | ACCEPT | Summary: Piwi is a nuclear PIWI protein. Reason: Core location: piRNA-loaded Piwi is imported into the nucleus, where it directs co-transcriptional silencing. Supporting Evidence: PMID:22902557 Formation of mature Piwi-RISC triggers its nuclear import, while failure in piRNA biogenesis results in destabilization of presumably unloaded Piwi PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0005634 nucleus | IDA PMID:29735528 A critical role for nucleoporin 358 (Nup358) in transposon s... | ACCEPT | Summary: Piwi is a nuclear PIWI protein. Reason: Core location: piRNA-loaded Piwi is imported into the nucleus, where it directs co-transcriptional silencing. Supporting Evidence: PMID:22902557 Formation of mature Piwi-RISC triggers its nuclear import, while failure in piRNA biogenesis results in destabilization of presumably unloaded Piwi PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0005634 nucleus | IDA PMID:31123065 Daedalus and Gasz recruit Armitage to mitochondria, bringing... | ACCEPT | Summary: Piwi is a nuclear PIWI protein. Reason: Core location: piRNA-loaded Piwi is imported into the nucleus, where it directs co-transcriptional silencing. Supporting Evidence: PMID:22902557 Formation of mature Piwi-RISC triggers its nuclear import, while failure in piRNA biogenesis results in destabilization of presumably unloaded Piwi PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0005634 nucleus | IDA PMID:31384064 The nascent RNA binding complex SFiNX licenses piRNA-guided ... | ACCEPT | Summary: Piwi is a nuclear PIWI protein. Reason: Core location: piRNA-loaded Piwi is imported into the nucleus, where it directs co-transcriptional silencing. Supporting Evidence: PMID:22902557 Formation of mature Piwi-RISC triggers its nuclear import, while failure in piRNA biogenesis results in destabilization of presumably unloaded Piwi PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0005634 nucleus | IDA PMID:33376130 Modulation of Aub-TDRD interactions elucidates piRNA amplifi... | ACCEPT | Summary: Piwi is a nuclear PIWI protein. Reason: Core location: piRNA-loaded Piwi is imported into the nucleus, where it directs co-transcriptional silencing. Supporting Evidence: PMID:22902557 Formation of mature Piwi-RISC triggers its nuclear import, while failure in piRNA biogenesis results in destabilization of presumably unloaded Piwi PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0005634 nucleus | IEA GO_REF:0000044 | ACCEPT | Summary: Piwi is a nuclear PIWI protein. Reason: Core location: piRNA-loaded Piwi is imported into the nucleus, where it directs co-transcriptional silencing. Supporting Evidence: PMID:22902557 Formation of mature Piwi-RISC triggers its nuclear import, while failure in piRNA biogenesis results in destabilization of presumably unloaded Piwi PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0005654 nucleoplasm | IDA PMID:10631171 piwi encodes a nucleoplasmic factor whose activity modulates... | ACCEPT | Summary: Nucleoplasm. Reason: Piwi is a nucleoplasmic protein in somatic and germline cells. Supporting Evidence: PMID:10631171 piwi encodes a novel nucleoplasmic protein present in both somatic and germline cells |
| GO:0005654 nucleoplasm | IDA PMID:19541914 Abundant primary piRNAs, endo-siRNAs, and microRNAs in a Dro... | ACCEPT | Summary: Nucleoplasm. Reason: Piwi is a nucleoplasmic protein in somatic and germline cells. Supporting Evidence: PMID:10631171 piwi encodes a novel nucleoplasmic protein present in both somatic and germline cells |
| GO:0005654 nucleoplasm | IEA GO_REF:0000044 | ACCEPT | Summary: Nucleoplasm. Reason: Piwi is a nucleoplasmic protein in somatic and germline cells. Supporting Evidence: PMID:10631171 piwi encodes a novel nucleoplasmic protein present in both somatic and germline cells |
| GO:0005694 chromosome | EXP PMID:28472469 Piwi interacts with chromatin at nuclear pores and promiscuo... | ACCEPT | Summary: Chromosome. Reason: Piwi associates with chromatin at target loci. Supporting Evidence: PMID:23392610 Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. |
| GO:0005694 chromosome | IEA GO_REF:0000044 | ACCEPT | Summary: Chromosome. Reason: Piwi associates with chromatin at target loci. Supporting Evidence: PMID:23392610 Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. |
| GO:0005737 cytoplasm | IDA PMID:21831924 Vreteno, a gonad-specific protein, is essential for germline... | KEEP AS NON CORE | Summary: Cytoplasmic Piwi. Reason: Piwi is loaded in the cytoplasm (Yb bodies, mitochondrial surface, nuage) before nuclear import; cytoplasmic presence is transient and not the effector site. Supporting Evidence: PMID:31833223 Here, we show that Armi is necessary for Piwi-pre-piRISC formation at Yb bodies |
| GO:0005737 cytoplasm | IDA PMID:26494711 Silencio/CG9754 connects the Piwi-piRNA complex to the cellu... | KEEP AS NON CORE | Summary: Cytoplasmic Piwi. Reason: Piwi is loaded in the cytoplasm (Yb bodies, mitochondrial surface, nuage) before nuclear import; cytoplasmic presence is transient and not the effector site. Supporting Evidence: PMID:31833223 Here, we show that Armi is necessary for Piwi-pre-piRISC formation at Yb bodies |
| GO:0005737 cytoplasm | IDA PMID:26808625 Tudor-SN Interacts with Piwi Antagonistically in Regulating ... | KEEP AS NON CORE | Summary: Cytoplasmic Piwi. Reason: Piwi is loaded in the cytoplasm (Yb bodies, mitochondrial surface, nuage) before nuclear import; cytoplasmic presence is transient and not the effector site. Supporting Evidence: PMID:31833223 Here, we show that Armi is necessary for Piwi-pre-piRISC formation at Yb bodies |
| GO:0005737 cytoplasm | IDA PMID:29735528 A critical role for nucleoporin 358 (Nup358) in transposon s... | KEEP AS NON CORE | Summary: Cytoplasmic Piwi. Reason: Piwi is loaded in the cytoplasm (Yb bodies, mitochondrial surface, nuage) before nuclear import; cytoplasmic presence is transient and not the effector site. Supporting Evidence: PMID:31833223 Here, we show that Armi is necessary for Piwi-pre-piRISC formation at Yb bodies |
| GO:0005737 cytoplasm | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: Cytoplasmic Piwi. Reason: Piwi is loaded in the cytoplasm (Yb bodies, mitochondrial surface, nuage) before nuclear import; cytoplasmic presence is transient and not the effector site. Supporting Evidence: PMID:31833223 Here, we show that Armi is necessary for Piwi-pre-piRISC formation at Yb bodies |
| GO:0005829 cytosol | HDA PMID:25294944 Subcellular localisations of the CPTI collection of YFP-tagg... | KEEP AS NON CORE | Summary: Cytosolic Piwi. Reason: Reflects the cytoplasmic loading stage or unloaded Piwi; not the effector site. Supporting Evidence: PMID:31833223 Here, we show that Armi is necessary for Piwi-pre-piRISC formation at Yb bodies |
| GO:0005829 cytosol | IDA PMID:31123065 Daedalus and Gasz recruit Armitage to mitochondria, bringing... | KEEP AS NON CORE | Summary: Cytosolic Piwi. Reason: Reflects the cytoplasmic loading stage or unloaded Piwi; not the effector site. Supporting Evidence: PMID:31833223 Here, we show that Armi is necessary for Piwi-pre-piRISC formation at Yb bodies |
| GO:0005829 cytosol | IDA PMID:31833223 Armitage determines Piwi-piRISC processing from precursor fo... | KEEP AS NON CORE | Summary: Cytosolic Piwi. Reason: Reflects the cytoplasmic loading stage or unloaded Piwi; not the effector site. Supporting Evidence: PMID:31833223 Here, we show that Armi is necessary for Piwi-pre-piRISC formation at Yb bodies |
| GO:0007279 pole cell formation | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Maternal Piwi is required for pole cell formation. Reason: Maternal Piwi is a polar granule component needed for PGC formation; a developmental role distinct from nuclear TE silencing. Supporting Evidence: PMID:16949822 PIWI is a maternal component of the polar granule, a germ-plasm-specific organelle essential for germline specification. |
| GO:0007279 pole cell formation | IMP PMID:16949822 The role of PIWI and the miRNA machinery in Drosophila germl... | KEEP AS NON CORE | Summary: Maternal Piwi is required for pole cell formation. Reason: Maternal Piwi is a polar granule component needed for PGC formation; a developmental role distinct from nuclear TE silencing. Supporting Evidence: PMID:16949822 PIWI is a maternal component of the polar granule, a germ-plasm-specific organelle essential for germline specification. |
| GO:0007283 spermatogenesis | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: Piwi regulates spermatogenesis. Reason: Piwi is required for male germline function and transposon control; a downstream developmental role. Supporting Evidence: PMID:26808625 Here we report that Piwi interacts with Tudor-SN (Tudor staphylococcal nuclease, TSN) antagonistically in regulating spermatogenesis but synergistically in silencing transposons. |
| GO:0007283 spermatogenesis | IMP PMID:26808625 Tudor-SN Interacts with Piwi Antagonistically in Regulating ... | KEEP AS NON CORE | Summary: Piwi regulates spermatogenesis. Reason: Piwi is required for male germline function and transposon control; a downstream developmental role. Supporting Evidence: PMID:26808625 Here we report that Piwi interacts with Tudor-SN (Tudor staphylococcal nuclease, TSN) antagonistically in regulating spermatogenesis but synergistically in silencing transposons. |
| GO:0007294 germarium-derived oocyte fate determination | IMP PMID:24658126 Piwi is required in multiple cell types to control germline ... | KEEP AS NON CORE | Summary: Piwi controls germline differentiation (PMID:24658126). Reason: Developmental requirement in escort cells and germline; non-core. Supporting Evidence: PMID:24658126 In this study, we show that piwi is required in ECs and germline to control germ cell differentiation. |
| GO:0009994 oocyte differentiation | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Oocyte differentiation (IEA). Reason: Developmental requirement downstream of piRNA function. |
| GO:0010369 chromocenter | IDA PMID:17875665 Drosophila PIWI associates with chromatin and interacts dire... | KEEP AS NON CORE | Summary: Chromocenter localization (PMID:17875665). Reason: Piwi colocalizes with HP1a at the chromocenter; a localization detail of its heterochromatin association. Supporting Evidence: PMID:17875665 These findings implicate a direct interaction between the PIWI-mediated small RNA mechanism and heterochromatin-forming pathways in determining the epigenetic state of the fly genome. |
| GO:0010526 transposable element silencing | IEA GO_REF:0000117 | ACCEPT | Summary: Transposable element silencing. Reason: Core BP. Piwi is required for TE repression in somatic and germline ovarian cells and in the male germline. Supporting Evidence: PMID:23392610 Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. PMID:24658126 Piwi is required in multiple cell types to control germline stem cell lineage development in the Drosophila ovary. |
| GO:0010526 transposable element silencing | IMP PMID:15817569 Argonaute protein PIWI controls mobilization of retrotranspo... | ACCEPT | Summary: Transposable element silencing. Reason: Core BP. Piwi is required for TE repression in somatic and germline ovarian cells and in the male germline. Supporting Evidence: PMID:23392610 Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. PMID:24658126 Piwi is required in multiple cell types to control germline stem cell lineage development in the Drosophila ovary. |
| GO:0010526 transposable element silencing | IMP PMID:24658126 Piwi is required in multiple cell types to control germline ... | ACCEPT | Summary: Transposable element silencing. Reason: Core BP. Piwi is required for TE repression in somatic and germline ovarian cells and in the male germline. Supporting Evidence: PMID:23392610 Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. PMID:24658126 Piwi is required in multiple cell types to control germline stem cell lineage development in the Drosophila ovary. |
| GO:0016441 post-transcriptional gene silencing | IMP PMID:11864605 RNAi related mechanisms affect both transcriptional and post... | KEEP AS NON CORE | Summary: Piwi mutations block transgene co-suppression (PTGS). Reason: Early genetic study; Piwi primarily mediates co-transcriptional silencing, and the PTGS effect is likely indirect. Supporting Evidence: PMID:11864605 Mutations in piwi, which belongs to a gene family with members required for RNAi, block PTGS and one aspect of TGS, indicating a connection between the two types of silencing. |
| GO:0030718 germ-line stem cell population maintenance | IDA PMID:10631171 piwi encodes a nucleoplasmic factor whose activity modulates... | KEEP AS NON CORE | Summary: Germ-line stem cell maintenance. Reason: Piwi in somatic niche cells and germline is required for GSC maintenance; a developmental role that is separable from TE silencing. Supporting Evidence: PMID:10631171 In the Drosophila ovary, piwi is required in somatic signaling cells to maintain germline stem cells. |
| GO:0031047 regulatory ncRNA-mediated gene silencing | IBA GO_REF:0000033 | ACCEPT | Summary: piRNA-guided gene silencing. Reason: Core: Piwi-piRNA complexes silence complementary loci. Supporting Evidence: PMID:23392610 Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. |
| GO:0031047 regulatory ncRNA-mediated gene silencing | IMP PMID:23392610 Piwi induces piRNA-guided transcriptional silencing and esta... | ACCEPT | Summary: piRNA-guided gene silencing. Reason: Core: Piwi-piRNA complexes silence complementary loci. Supporting Evidence: PMID:23392610 Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. |
| GO:0031490 chromatin DNA binding | IDA PMID:28472469 Piwi interacts with chromatin at nuclear pores and promiscuo... | MARK AS OVER ANNOTATED | Summary: Chromatin DNA binding, from DamID (PMID:28472469). Reason: DamID detects proximity of Piwi to chromatin, not direct DNA binding; the paper itself notes Piwi is thought to contact chromatin via nascent RNA rather than DNA. Chromatin association is captured by CC terms. Supporting Evidence: PMID:28472469 Additionally, there is some evidence indicating that Piwi interacts with chromatin regions via RNA, but not DNA |
| GO:0031507 heterochromatin formation | IEA GO_REF:0000117 | ACCEPT | Summary: Heterochromatin formation. Reason: Piwi-piRNA complexes induce H3K9me3/HP1a heterochromatin at targets; the more specific GO:0141006 is also annotated. Supporting Evidence: PMID:23392610 Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. PMID:17875665 These findings implicate a direct interaction between the PIWI-mediated small RNA mechanism and heterochromatin-forming pathways in determining the epigenetic state of the fly genome. |
| GO:0031507 heterochromatin formation | IMP PMID:11864605 RNAi related mechanisms affect both transcriptional and post... | ACCEPT | Summary: Heterochromatin formation. Reason: Piwi-piRNA complexes induce H3K9me3/HP1a heterochromatin at targets; the more specific GO:0141006 is also annotated. Supporting Evidence: PMID:23392610 Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. PMID:17875665 These findings implicate a direct interaction between the PIWI-mediated small RNA mechanism and heterochromatin-forming pathways in determining the epigenetic state of the fly genome. |
| GO:0031507 heterochromatin formation | IMP PMID:14752161 Heterochromatic silencing and HP1 localization in Drosophila... | ACCEPT | Summary: Heterochromatin formation. Reason: Piwi-piRNA complexes induce H3K9me3/HP1a heterochromatin at targets; the more specific GO:0141006 is also annotated. Supporting Evidence: PMID:23392610 Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. PMID:17875665 These findings implicate a direct interaction between the PIWI-mediated small RNA mechanism and heterochromatin-forming pathways in determining the epigenetic state of the fly genome. |
| GO:0031507 heterochromatin formation | IMP PMID:16530043 RNAi components are required for nuclear clustering of Polyc... | ACCEPT | Summary: Heterochromatin formation. Reason: Piwi-piRNA complexes induce H3K9me3/HP1a heterochromatin at targets; the more specific GO:0141006 is also annotated. Supporting Evidence: PMID:23392610 Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. PMID:17875665 These findings implicate a direct interaction between the PIWI-mediated small RNA mechanism and heterochromatin-forming pathways in determining the epigenetic state of the fly genome. |
| GO:0031507 heterochromatin formation | IMP PMID:17875665 Drosophila PIWI associates with chromatin and interacts dire... | ACCEPT | Summary: Heterochromatin formation. Reason: Piwi-piRNA complexes induce H3K9me3/HP1a heterochromatin at targets; the more specific GO:0141006 is also annotated. Supporting Evidence: PMID:23392610 Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. PMID:17875665 These findings implicate a direct interaction between the PIWI-mediated small RNA mechanism and heterochromatin-forming pathways in determining the epigenetic state of the fly genome. |
| GO:0032991 protein-containing complex | IDA PMID:29531043 Structural insights into the sequence-specific recognition o... | MARK AS OVER ANNOTATED | Summary: Protein-containing complex (Piwi-Papi; PMID:29531043). Reason: Uninformative root complex term. |
| GO:0034584 piRNA binding | IBA GO_REF:0000033 | ACCEPT | Summary: piRNA binding. Reason: Core MF: Piwi binds antisense, 5-U piRNAs whose 3 ends are 2-O-methylated by Pimet/Hen1. Supporting Evidence: PMID:16882972 These data together imply that Piwi functions in nuclear RNA silencing as Slicer by associating specifically with rasiRNAs originating from repetitive targets. PMID:17346786 Piwi and Aubergine bind RNAs that are predominantly antisense to transposons |
| GO:0034584 piRNA binding | IDA PMID:17346786 Discrete small RNA-generating loci as master regulators of t... | ACCEPT | Summary: piRNA binding. Reason: Core MF: Piwi binds antisense, 5-U piRNAs whose 3 ends are 2-O-methylated by Pimet/Hen1. Supporting Evidence: PMID:16882972 These data together imply that Piwi functions in nuclear RNA silencing as Slicer by associating specifically with rasiRNAs originating from repetitive targets. PMID:17346786 Piwi and Aubergine bind RNAs that are predominantly antisense to transposons |
| GO:0034584 piRNA binding | IDA PMID:17606638 Pimet, the Drosophila homolog of HEN1, mediates 2'-O-methyla... | ACCEPT | Summary: piRNA binding. Reason: Core MF: Piwi binds antisense, 5-U piRNAs whose 3 ends are 2-O-methylated by Pimet/Hen1. Supporting Evidence: PMID:16882972 These data together imply that Piwi functions in nuclear RNA silencing as Slicer by associating specifically with rasiRNAs originating from repetitive targets. PMID:17346786 Piwi and Aubergine bind RNAs that are predominantly antisense to transposons |
| GO:0034584 piRNA binding | IDA PMID:26494711 Silencio/CG9754 connects the Piwi-piRNA complex to the cellu... | ACCEPT | Summary: piRNA binding. Reason: Core MF: Piwi binds antisense, 5-U piRNAs whose 3 ends are 2-O-methylated by Pimet/Hen1. Supporting Evidence: PMID:16882972 These data together imply that Piwi functions in nuclear RNA silencing as Slicer by associating specifically with rasiRNAs originating from repetitive targets. PMID:17346786 Piwi and Aubergine bind RNAs that are predominantly antisense to transposons |
| GO:0034584 piRNA binding | IDA PMID:31123065 Daedalus and Gasz recruit Armitage to mitochondria, bringing... | ACCEPT | Summary: piRNA binding. Reason: Core MF: Piwi binds antisense, 5-U piRNAs whose 3 ends are 2-O-methylated by Pimet/Hen1. Supporting Evidence: PMID:16882972 These data together imply that Piwi functions in nuclear RNA silencing as Slicer by associating specifically with rasiRNAs originating from repetitive targets. PMID:17346786 Piwi and Aubergine bind RNAs that are predominantly antisense to transposons |
| GO:0034584 piRNA binding | IDA PMID:31833223 Armitage determines Piwi-piRISC processing from precursor fo... | ACCEPT | Summary: piRNA binding. Reason: Core MF: Piwi binds antisense, 5-U piRNAs whose 3 ends are 2-O-methylated by Pimet/Hen1. Supporting Evidence: PMID:16882972 These data together imply that Piwi functions in nuclear RNA silencing as Slicer by associating specifically with rasiRNAs originating from repetitive targets. PMID:17346786 Piwi and Aubergine bind RNAs that are predominantly antisense to transposons |
| GO:0034584 piRNA binding | IEA GO_REF:0000117 | ACCEPT | Summary: piRNA binding. Reason: Core MF: Piwi binds antisense, 5-U piRNAs whose 3 ends are 2-O-methylated by Pimet/Hen1. Supporting Evidence: PMID:16882972 These data together imply that Piwi functions in nuclear RNA silencing as Slicer by associating specifically with rasiRNAs originating from repetitive targets. PMID:17346786 Piwi and Aubergine bind RNAs that are predominantly antisense to transposons |
| GO:0034584 piRNA binding | IMP PMID:31833223 Armitage determines Piwi-piRISC processing from precursor fo... | ACCEPT | Summary: piRNA binding. Reason: Core MF: Piwi binds antisense, 5-U piRNAs whose 3 ends are 2-O-methylated by Pimet/Hen1. Supporting Evidence: PMID:16882972 These data together imply that Piwi functions in nuclear RNA silencing as Slicer by associating specifically with rasiRNAs originating from repetitive targets. PMID:17346786 Piwi and Aubergine bind RNAs that are predominantly antisense to transposons |
| GO:0034587 piRNA processing | IBA GO_REF:0000033 | ACCEPT | Summary: piRNA processing. Reason: Piwi loading drives primary (phased) piRNA biogenesis; unloaded Piwi is unstable. Supporting Evidence: PMID:22902557 Formation of mature Piwi-RISC triggers its nuclear import, while failure in piRNA biogenesis results in destabilization of presumably unloaded Piwi |
| GO:0034587 piRNA processing | IEA GO_REF:0000117 | ACCEPT | Summary: piRNA processing. Reason: Piwi loading drives primary (phased) piRNA biogenesis; unloaded Piwi is unstable. Supporting Evidence: PMID:22902557 Formation of mature Piwi-RISC triggers its nuclear import, while failure in piRNA biogenesis results in destabilization of presumably unloaded Piwi |
| GO:0043186 P granule | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: Nuage/polar granule (P granule). Reason: Maternal Piwi is a polar granule component, but Piwi is predominantly nuclear in ovaries; the nuage is not its effector site. Supporting Evidence: PMID:16949822 PIWI is a maternal component of the polar granule |
| GO:0043186 P granule | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: Nuage/polar granule (P granule). Reason: Maternal Piwi is a polar granule component, but Piwi is predominantly nuclear in ovaries; the nuage is not its effector site. Supporting Evidence: PMID:16949822 PIWI is a maternal component of the polar granule |
| GO:0048132 female germ-line stem cell asymmetric division | IMP PMID:9851978 A novel class of evolutionarily conserved genes defined by p... | KEEP AS NON CORE | Summary: Female GSC asymmetric division. Reason: Classic developmental function (Cox 1998), separable from TE silencing. Supporting Evidence: PMID:9851978 it is required for the asymmetric division of GSCs to produce and maintain a daughter GSC |
| GO:0048133 male germ-line stem cell asymmetric division | IMP PMID:9851978 A novel class of evolutionarily conserved genes defined by p... | KEEP AS NON CORE | Summary: Male GSC asymmetric division. Reason: Classic developmental function (Cox 1998). Supporting Evidence: PMID:9851978 it is required for the asymmetric division of GSCs to produce and maintain a daughter GSC |
| GO:0048477 oogenesis | IMP PMID:26808625 Tudor-SN Interacts with Piwi Antagonistically in Regulating ... | KEEP AS NON CORE | Summary: Oogenesis. Reason: piwi mutants have severely underdeveloped ovaries; developmental consequence. Supporting Evidence: PMID:22065765 Hence, null mutant piwi females exhibit two types of abnormalities, overexpression of transposons and severely underdeveloped ovaries. |
| GO:0070725 Yb body | IDA PMID:22902557 The cochaperone shutdown defines a group of biogenesis facto... | ACCEPT | Summary: Yb body. Reason: Piwi-pre-piRISC forms at Yb bodies in ovarian somatic cells before nuclear import. Supporting Evidence: PMID:31833223 Here, we show that Armi is necessary for Piwi-pre-piRISC formation at Yb bodies |
| GO:0070725 Yb body | IDA PMID:31123065 Daedalus and Gasz recruit Armitage to mitochondria, bringing... | ACCEPT | Summary: Yb body. Reason: Piwi-pre-piRISC forms at Yb bodies in ovarian somatic cells before nuclear import. Supporting Evidence: PMID:31833223 Here, we show that Armi is necessary for Piwi-pre-piRISC formation at Yb bodies |
| GO:0070725 Yb body | IMP PMID:31833223 Armitage determines Piwi-piRISC processing from precursor fo... | ACCEPT | Summary: Yb body. Reason: Piwi-pre-piRISC forms at Yb bodies in ovarian somatic cells before nuclear import. Supporting Evidence: PMID:31833223 Here, we show that Armi is necessary for Piwi-pre-piRISC formation at Yb bodies |
| GO:0070725 Yb body | IPI PMID:21863019 A systematic analysis of Drosophila TUDOR domain-containing ... | ACCEPT | Summary: Yb body. Reason: Piwi-pre-piRISC forms at Yb bodies in ovarian somatic cells before nuclear import. Supporting Evidence: PMID:31833223 Here, we show that Armi is necessary for Piwi-pre-piRISC formation at Yb bodies |
| GO:0140990 primary piRNA processing | IMP PMID:22902557 The cochaperone shutdown defines a group of biogenesis facto... | ACCEPT | Summary: Primary piRNA processing. Reason: Piwi is the acceptor of primary piRNAs in the somatic and phased pathway; loading is coupled to Zucchini-dependent processing. Supporting Evidence: PMID:31833223 Here, we show that Armi is necessary for Piwi-pre-piRISC formation at Yb bodies PMID:22902557 Formation of mature Piwi-RISC triggers its nuclear import, while failure in piRNA biogenesis results in destabilization of presumably unloaded Piwi |
| GO:0140990 primary piRNA processing | IMP PMID:26494711 Silencio/CG9754 connects the Piwi-piRNA complex to the cellu... | ACCEPT | Summary: Primary piRNA processing. Reason: Piwi is the acceptor of primary piRNAs in the somatic and phased pathway; loading is coupled to Zucchini-dependent processing. Supporting Evidence: PMID:31833223 Here, we show that Armi is necessary for Piwi-pre-piRISC formation at Yb bodies PMID:22902557 Formation of mature Piwi-RISC triggers its nuclear import, while failure in piRNA biogenesis results in destabilization of presumably unloaded Piwi |
| GO:0140990 primary piRNA processing | IMP PMID:31833223 Armitage determines Piwi-piRISC processing from precursor fo... | ACCEPT | Summary: Primary piRNA processing. Reason: Piwi is the acceptor of primary piRNAs in the somatic and phased pathway; loading is coupled to Zucchini-dependent processing. Supporting Evidence: PMID:31833223 Here, we show that Armi is necessary for Piwi-pre-piRISC formation at Yb bodies PMID:22902557 Formation of mature Piwi-RISC triggers its nuclear import, while failure in piRNA biogenesis results in destabilization of presumably unloaded Piwi |
| GO:0140991 piRNA-mediated gene silencing by mRNA destabilization | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: piRNA-mediated mRNA destabilization. Reason: Rouget et al. implicate piRNA pathway components including piwi in maternal mRNA decay; for Piwi, the main mechanism is transcriptional, so this is non-core. Supporting Evidence: PMID:20953170 Consistent with earlier mentioned defects7, we found that the piwi1 mutant embryos which were able to produce a cuticle had strong head defects |
| GO:0140991 piRNA-mediated gene silencing by mRNA destabilization | IMP PMID:20953170 Maternal mRNA deadenylation and decay by the piRNA pathway i... | KEEP AS NON CORE | Summary: piRNA-mediated mRNA destabilization. Reason: Rouget et al. implicate piRNA pathway components including piwi in maternal mRNA decay; for Piwi, the main mechanism is transcriptional, so this is non-core. Supporting Evidence: PMID:20953170 Consistent with earlier mentioned defects7, we found that the piwi1 mutant embryos which were able to produce a cuticle had strong head defects |
| GO:0141005 transposable element silencing by heterochromatin formation | IMP PMID:26494711 Silencio/CG9754 connects the Piwi-piRNA complex to the cellu... | ACCEPT | Summary: TE silencing by heterochromatin formation. Reason: Core BP. Supporting Evidence: PMID:23392610 Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. |
| GO:0141006 transposable element silencing by piRNA-mediated heterochromatin formation | IMP PMID:22065765 Separation of stem cell maintenance and transposon silencing... | ACCEPT | Summary: Piwi is required for piRNA-guided heterochromatin formation at transposons (PMID:22065765). Reason: Core BP. Nuclear Piwi-piRNA complexes recognize nascent transposon transcripts and recruit the Panoramix/SFiNX-SUMO-Eggless/SetDB1 machinery to deposit H3K9me3. Supporting Evidence: PMID:23392610 Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0141006 transposable element silencing by piRNA-mediated heterochromatin formation | IMP PMID:22160707 Drosophila Piwi functions downstream of piRNA production med... | ACCEPT | Summary: Piwi is required for piRNA-guided heterochromatin formation at transposons (PMID:22160707). Reason: Core BP. Nuclear Piwi-piRNA complexes recognize nascent transposon transcripts and recruit the Panoramix/SFiNX-SUMO-Eggless/SetDB1 machinery to deposit H3K9me3. Supporting Evidence: PMID:23392610 Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0141006 transposable element silencing by piRNA-mediated heterochromatin formation | IMP PMID:23159368 Transcriptional silencing of transposons by Piwi and maelstr... | ACCEPT | Summary: Piwi is required for piRNA-guided heterochromatin formation at transposons (PMID:23159368). Reason: Core BP. Nuclear Piwi-piRNA complexes recognize nascent transposon transcripts and recruit the Panoramix/SFiNX-SUMO-Eggless/SetDB1 machinery to deposit H3K9me3. Supporting Evidence: PMID:23392610 Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0141006 transposable element silencing by piRNA-mediated heterochromatin formation | IMP PMID:26472911 Panoramix enforces piRNA-dependent cotranscriptional silenci... | ACCEPT | Summary: Piwi is required for piRNA-guided heterochromatin formation at transposons (PMID:26472911). Reason: Core BP. Nuclear Piwi-piRNA complexes recognize nascent transposon transcripts and recruit the Panoramix/SFiNX-SUMO-Eggless/SetDB1 machinery to deposit H3K9me3. Supporting Evidence: PMID:23392610 Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0141006 transposable element silencing by piRNA-mediated heterochromatin formation | IMP PMID:26494711 Silencio/CG9754 connects the Piwi-piRNA complex to the cellu... | ACCEPT | Summary: Piwi is required for piRNA-guided heterochromatin formation at transposons (PMID:26494711). Reason: Core BP. Nuclear Piwi-piRNA complexes recognize nascent transposon transcripts and recruit the Panoramix/SFiNX-SUMO-Eggless/SetDB1 machinery to deposit H3K9me3. Supporting Evidence: PMID:23392610 Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0141006 transposable element silencing by piRNA-mediated heterochromatin formation | IMP PMID:28457744 Piwi Is Required during Drosophila Embryogenesis to License ... | ACCEPT | Summary: Piwi is required for piRNA-guided heterochromatin formation at transposons (PMID:28457744). Reason: Core BP. Nuclear Piwi-piRNA complexes recognize nascent transposon transcripts and recruit the Panoramix/SFiNX-SUMO-Eggless/SetDB1 machinery to deposit H3K9me3. Supporting Evidence: PMID:23392610 Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0141006 transposable element silencing by piRNA-mediated heterochromatin formation | IMP PMID:30527661 Maelstrom Represses Canonical Polymerase II Transcription wi... | ACCEPT | Summary: Piwi is required for piRNA-guided heterochromatin formation at transposons (PMID:30527661). Reason: Core BP. Nuclear Piwi-piRNA complexes recognize nascent transposon transcripts and recruit the Panoramix/SFiNX-SUMO-Eggless/SetDB1 machinery to deposit H3K9me3. Supporting Evidence: PMID:23392610 Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0141006 transposable element silencing by piRNA-mediated heterochromatin formation | IMP PMID:32499524 The Mi-2 nucleosome remodeler and the Rpd3 histone deacetyla... | ACCEPT | Summary: Piwi is required for piRNA-guided heterochromatin formation at transposons (PMID:32499524). Reason: Core BP. Nuclear Piwi-piRNA complexes recognize nascent transposon transcripts and recruit the Panoramix/SFiNX-SUMO-Eggless/SetDB1 machinery to deposit H3K9me3. Supporting Evidence: PMID:23392610 Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0141006 transposable element silencing by piRNA-mediated heterochromatin formation | IMP PMID:33538693 Dimerisation of the PICTS complex via LC8/Cut-up drives co-t... | ACCEPT | Summary: Piwi is required for piRNA-guided heterochromatin formation at transposons (PMID:33538693). Reason: Core BP. Nuclear Piwi-piRNA complexes recognize nascent transposon transcripts and recruit the Panoramix/SFiNX-SUMO-Eggless/SetDB1 machinery to deposit H3K9me3. Supporting Evidence: PMID:23392610 Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0141006 transposable element silencing by piRNA-mediated heterochromatin formation | IMP PMID:34236313 Maternally inherited piRNAs direct transient heterochromatin... | ACCEPT | Summary: Piwi is required for piRNA-guided heterochromatin formation at transposons (PMID:34236313). Reason: Core BP. Nuclear Piwi-piRNA complexes recognize nascent transposon transcripts and recruit the Panoramix/SFiNX-SUMO-Eggless/SetDB1 machinery to deposit H3K9me3. Supporting Evidence: PMID:23392610 Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0141006 transposable element silencing by piRNA-mediated heterochromatin formation | IMP PMID:34337769 Piwi-piRNA complexes induce stepwise changes in nuclear arch... | ACCEPT | Summary: Piwi is required for piRNA-guided heterochromatin formation at transposons (PMID:34337769). Reason: Core BP. Nuclear Piwi-piRNA complexes recognize nascent transposon transcripts and recruit the Panoramix/SFiNX-SUMO-Eggless/SetDB1 machinery to deposit H3K9me3. Supporting Evidence: PMID:23392610 Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0141006 transposable element silencing by piRNA-mediated heterochromatin formation | IMP PMID:35173350 Panoramix SUMOylation on chromatin connects the piRNA pathwa... | ACCEPT | Summary: Piwi is required for piRNA-guided heterochromatin formation at transposons (PMID:35173350). Reason: Core BP. Nuclear Piwi-piRNA complexes recognize nascent transposon transcripts and recruit the Panoramix/SFiNX-SUMO-Eggless/SetDB1 machinery to deposit H3K9me3. Supporting Evidence: PMID:23392610 Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
| GO:0141006 transposable element silencing by piRNA-mediated heterochromatin formation | IMP PMID:38308815 Direct interaction of Su(var)2-10 via the SIM-binding site o... | ACCEPT | Summary: Piwi is required for piRNA-guided heterochromatin formation at transposons (PMID:38308815). Reason: Core BP. Nuclear Piwi-piRNA complexes recognize nascent transposon transcripts and recruit the Panoramix/SFiNX-SUMO-Eggless/SetDB1 machinery to deposit H3K9me3. Supporting Evidence: PMID:23392610 Our results indicate that Piwi identifies targets complementary to the associated piRNA and induces transcriptional repression by establishing a repressive chromatin state when correct targets are found. PMID:26472911 In Drosophila ovaries, the nuclear Piwi protein is required for transcriptional silencing of transposons, though the precise mechanisms by which this occurs are unknown. |
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Download this section (compressed HTML)Q: How does Piwi-piRISC engagement of a nascent transcript signal to Asterix/Panoramix, given that Piwi slicer activity is dispensable?
Q: Is the GSC-maintenance function of Piwi piRNA-dependent, given that it does not require nuclear localization?
Experiment: Cryo-EM of Piwi-piRNA-target RNA complexes with Asterix, combined with surface mutants tested for silencing in OSCs.
Hypothesis: A target-engaged conformation of Piwi exposes a surface recognized by Asterix/Panoramix.
Type: Structure-function analysis
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