ProtNLM2 External predictions
View prediction review YAML · A0A6I8W8A2-protnlm-predictions-review.yaml · Review status: COMPLETE
The 169-residue RCC1-like sequence (LOC117183218 isoform X3) does not contain a ligase catalytic module, and no product of the gene has a HECT domain.
Source documents: genes/DROPS/A0A6I8W8A2/A0A6I8W8A2-uniprot.txt · genes/DROPS/A0A6I8W8A2/A0A6I8W8A2-goa.tsv · genes/DROPS/A0A6I8W8A2/A0A6I8W8A2-hypotheses/prediction-ligase-activity/openscientist.md
Review score: 2 = concordant with evidence; 1 = uncertain; 0 = discordant with evidence. This is an assessment score, not a model probability.
NPI — Nonparalog incorrect Review score: 0/2
Prediction method: ProtNLM2 · Version: UniProt 2024_06 pilot
DOMAIN ARCHITECTURE MISMATCH
Review rationale: The deposited sequence is a short RCC1-repeat protein with RCC1/BLIP-II fold assignments and no HECT or other ligase catalytic module. An RCC1 region from a larger HERC-like protein does not carry the catalytic activity of that protein's separate HECT domain. The prediction of intrinsic ligase activity is therefore incompatible with the available domain architecture. Sequence comparison of all products of LOC117183218 (the longest is 282 aa) finds no HECT domain in any of them, so the refutation holds at the gene level; the gene has no GO annotations, and the gene review records no established molecular function.
Supporting Evidence:
- file:DROPS/A0A6I8W8A2/A0A6I8W8A2-uniprot.txt: "ID A0A6I8W8A2_DROPS Unreviewed; 169 AA. ... DR InterPro; IPR009091; RCC1/BLIP-II. ... DR InterPro; IPR000408; Reg_chr_condens."
- file:DROPS/A0A6I8W8A2/A0A6I8W8A2-hypotheses/prediction-ligase-activity/openscientist.md: "none is a ligase catalytic signature"
- file:DROPS/A0A6I8W8A2/A0A6I8W8A2-bioinformatics/RESULTS.md: "No product of the gene has a HECT domain (the longest is 282 aa; a HECT domain alone is ~350 aa)."