id: B4MAQ2
gene_symbol: B4MAQ2
taxon:
  id: NCBITaxon:7244
  label: Drosophila virilis
status: COMPLETE
description: >-
  The Exportin-5 subfamily assignment supports nucleocytoplasmic shuttling and the existing cytoplasmic
  annotation.
source_documents:
  - genes/DROVI/B4MAQ2/B4MAQ2-uniprot.txt
  - genes/DROVI/B4MAQ2/B4MAQ2-goa.tsv
  - publications/PMID_39098529.md
  - genes/DROVI/B4MAQ2/B4MAQ2-hypotheses/prediction-cytoplasm-localization/openscientist.md
predictions:
  - source_method: ProtNLM2
    source_version: UniProt 2024_06 pilot
    predicted_term:
      id: GO:0005737
      label: cytoplasm
    predicted_term_type: GO_CC
    review:
      assessment: CNN
      confidence_score: 2
      summary: >-
        The target contains Exportin-5-specific domain and subfamily assignments across a full-length
        transport-receptor architecture. Experiments in Drosophila establish Exportin-5-dependent export
        of nuclear pre-tRNAs to the cytoplasm (PMID:39098529), supporting cytoplasmic residence during
        the conserved transport cycle. This is a family-based localization inference and does not imply
        exclusive cytoplasmic localization. Cytoplasm is already present in cached GOA and UniProt.
      supported_by:
        - reference_id: file:DROVI/B4MAQ2/B4MAQ2-uniprot.txt
          supporting_text: >-
            ID   B4MAQ2_DROVI            Unreviewed;      1238 AA. ... DR   GO; GO:0005737; C:cytoplasm;
            IEA:TreeGrafter. ... DR   InterPro; IPR045478; Exportin-5_C. ... DR   PANTHER; PTHR11223:SF3;
            EXPORTIN-5; 1. ... FT   DOMAIN          34..100 ... FT                   /note="Importin N-terminal"
            ... FT   DOMAIN          114..274 ... FT                   /note="Exportin-1/Importin-beta-like"
            ... FT   DOMAIN          319..1193 ... FT                   /note="Exportin-5 C-terminal"
        - reference_id: PMID:39098529
          supporting_text: >-
            pre-tRNAs before end-processing were present in the cytoplasm
        - reference_id: file:DROVI/B4MAQ2/B4MAQ2-hypotheses/prediction-cytoplasm-localization/openscientist.md
          supporting_text: obligatory cytoplasmic phase in its transport cycle
references:
  - id: file:DROVI/B4MAQ2/B4MAQ2-uniprot.txt
    title: B4MAQ2-uniprot.txt
  - id: PMID:39098529
    title: Exportin-5 binding precedes 5'- and 3'-end processing of tRNA precursors in Drosophila.
  - id: file:DROVI/B4MAQ2/B4MAQ2-hypotheses/prediction-cytoplasm-localization/openscientist.md
    title: Focused OpenScientist report on B4MAQ2 cytoplasm prediction
    reference_review:
      relevance: HIGH
      review_notes: >-
        Substantive computational synthesis directly addressing the cytoplasm prediction. The report's
        Exportin-5 family assignment agrees with the cached UniProt Exportin-5-specific InterPro and
        PANTHER records, and PMID:39098529 independently supports the existence of a cytoplasmic phase
        in the conserved Drosophila pre-tRNA export cycle. The report supports cytoplasmic residence
        during shuttling, not exclusive or constitutive cytoplasmic localization.