| Component/gene | PTS subunit/domain name | Localization/topology | Substrate scope | Additional roles | Key supporting citations with years/DOIs/URLs |
|---|---|---|---|---|---|
| **manX** | **EIIAB\_Man** (cytoplasmic phosphotransferase component) | Cytoplasmic/hydrophilic subunit; carries the phosphorylation sites used in PTS transfer to incoming sugar; identified as the cytoplasmic component of ManXYZ (pqac-00000009, pqac-00000010) | Mannose; also contributes to uptake/phosphorylation of glucose and glucose analogs via the mannose-family PTS, including α-methylglucoside and 2-deoxyglucose (pqac-00000008, pqac-00000010, pqac-00000012) | Not the membrane pore itself; phage λ penetration role is assigned mainly to the ManY/ManZ membrane subcomplex rather than ManX (pqac-00000009) | Huber & Erni 1996, *Eur J Biochem* 239:810-817, doi:10.1111/j.1432-1033.1996.0810u.x, https://doi.org/10.1111/j.1432-1033.1996.0810u.x (pqac-00000008); Rice & Vanderpool 2011, *Nucleic Acids Res* 39:3806-3819, doi:10.1093/nar/gkq1219, https://doi.org/10.1093/nar/gkq1219 (pqac-00000010) |
| **manY** | **EIIC\_Man** | Inner-membrane subunit; predicted to span the membrane **6 times**; both termini cytoplasmic in the topology model; forms a tight transmembrane complex with ManZ (pqac-00000008, pqac-00000009) | Broad mannose-family PTS specificity: mannose plus glucose/related hexoses; EIIMan also supports transport of glucose and analogs in *E. coli* physiology/reviews (pqac-00000008, pqac-00000010, pqac-00000012, pqac-00000014) | Together with ManZ, forms the membrane subcomplex sufficient for **bacteriophage λ DNA penetration** across the inner membrane (pqac-00000008, pqac-00000009) | Huber & Erni 1996, doi:10.1111/j.1432-1033.1996.0810u.x, https://doi.org/10.1111/j.1432-1033.1996.0810u.x (pqac-00000008); Esquinas-Rychen & Erni 2001, inner-membrane facilitation of λ DNA injection (URL/DOI not available in context) (pqac-00000009) |
| **manZ** | **EIID\_Man / IID-Man / EII-D** | Inner-membrane subunit of the ManXYZ complex; topology studies describe ManZ/IID as membrane-anchored by a **single C-terminal TM helix** with ~250 aa projecting into the cytoplasm; older evidence also describes IID as a membrane protein with **1 TM span** that complexes with ManY (pqac-00000001, pqac-00000008, pqac-00000009) | Part of the mannose-family PTS for coupled transport/phosphorylation of mannose and other related hexoses; through ManXYZ/EIIMan, also supports uptake of glucose and glucose analogs such as α-methylglucoside and 2-deoxyglucose (pqac-00000008, pqac-00000010, pqac-00000012, pqac-00000014) | **Primary additional role:** with ManY, constitutes the inner-membrane complex required/sufficient for **phage λ DNA injection**; the manXYZ locus is also linked to older **ptsM/pel** nomenclature for λ penetration phenotypes (pqac-00000000, pqac-00000008, pqac-00000009) | Huber & Erni 1996, *Eur J Biochem* 239:810-817, doi:10.1111/j.1432-1033.1996.0810u.x, https://doi.org/10.1111/j.1432-1033.1996.0810u.x (pqac-00000001, pqac-00000008); Esquinas-Rychen & Erni 2001, λ DNA injection study (URL/DOI not available in context) (pqac-00000000, pqac-00000009) |


*Table: This table summarizes the E. coli K-12 mannose-family PTS components with emphasis on ManZ (EIID-Man), including subunit assignment, membrane topology, substrate scope, and the additional role of the ManY/ManZ complex in phage λ DNA injection. It is useful as a concise evidence-backed annotation aid for functional and localization interpretation.*